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About Mini Preparation PROTOCOL
This document provides the protocol for a lab involving minipreparation of plasmid DNA from bacterial cultures, restriction digestion of the purified plasmids, and gel electrophoresis to analyze the results. Key steps include inoculating cultures overnight, harvesting and lysing the bacterial cells to isolate plasmid DNA, precipitating and washing the DNA, setting up restriction digests of the purified plasmids using selected enzymes, and running the digestion products on a gel for analysis and comparison to controls.
Detailed Information
| Author: | ['Keith Arora-Williams'] |
|---|---|
| Publication Year: | 2011 |
| Pages: | 3 |
| Language: | English |
| Format: | |
| Price: | FREE |
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