microbial population dynamics in vermicompost enriched with biofertilizers and their effect on black

167 Pages·2016·5.58 MB·English

MICROBIAL POPULATION
DYNAMICS IN VERMICOMPOST
ENRICHED WITH BIOFERTILIZERS
AND THEIR EFFECT ON
BLACK GRAM

HARISH KUMAR MEHTA
B.Sc. (Ag.)

MASTER OF SCIENCE IN AGRICULTURE
(AGRICULTURAL MICROBIOLOGY)

2012
MICROBIAL POPULATION DYNAMICS IN
VERMICOMPOST ENRICHED WITH
BIOFERTILIZERS AND THEIR EFFECT ON
BLACK GRAM
BY
HARISH KUMAR MEHTA
B.Sc. (Ag.)
THESIS SUBMITTED TO THE ACHARYA N.G. RANGA
AGRICULTURAL UNIVERSITY IN PARTIAL FULFILMENT OF
THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE
(AGRICULTURAL MICROBIOLOGY)

CHAIRPERSON: Dr. R.SUBHASH REDDY

DEPARTMENT OF AGRICULTURAL MICROBIOLOGY
COLLEGE OF AGRICULTURE
RAJENDRANAGAR HYDERABAD-500 030
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
2012
DECLARATION

I, HARISH KUMAR MEHTA, hereby declare that the thesis entitled,
“MICROBIAL POPULATION DYNAMICS IN VERMICOMPOST ENRICHED
WITH BIOFERTILIZERS AND THEIR EFFECT ON BLACK GRAM [Vigna
mungo (L.) Hepper]” submitted to Acharya N.G. Ranga Agricultural University for
the degree of MASTER OF SCIENCE IN AGRICULTURE in the major field of
Agricutural Microbiology, is the result of original research work done by me. I also
declare that no material contained in the thesis or any part thereof has not been
published earlier in any manner.

Date: (HARISH KUMAR MEHTA)

Place: Hyderabad I. D. No. RAM/2010-75
CERTIFICATE

Mr. HARISH KUMAR MEHTA has satisfactorily prosecuted the course of
research and the thesis entitled “MICROBIAL POPULATION DYNAMICS IN
VERMICOMPOST ENRICHED WITH BIOFERTILIZERS AND THEIR
EFFECT ON BLACK GRAM [Vigna mungo (L.) Hepper]” submitted is the result of
original research work and is of sufficiently high standard to warrant its presentation to
the examination. I also certify that neither the thesis nor its part thereof has been
previously submitted by him for a degree of any University.

Date: (Dr. R. SUBHASH REDDY)
Chairperson
CERTIFICATE
This is to certify that the thesis entitled “MICROBIAL POPULATION
DYNAMICS IN VERMICOMPOST ENRICHED WITH BIOFERTILIZERS AND
THEIR EFFECT ON BLACK GRAM [Vigna mungo (L.) Hepper]” submitted in partial
fulfillment of the requirements for the degree of “MASTER OF SCIENCE IN
AGRICUTLURE” of the Acharya N. G. Ranga Agricultural University, Hyderabad, is
a record of the bonafide research work carried out by Mr. HARISH KUMAR MEHTA,
under our guidance and supervision. The subject of the thesis has been approved by the
Student's Advisory Committee.
No part of the thesis has been submitted for any other degree or diploma. The
published part has been fully acknowledged. All assistance and help received during the
course of the investigation have been duly acknowledged by the author of the thesis.

CHAIRMAN
ADVISORY COMMITTEE

Thesis approved by the Student’s Advisory Committee

Chairman : ( Dr. R. SUBHASH REDDY)
Professor and University Head
Agricultural Microbiology and Bioenergy,
_________________
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030

Member : (Dr. S. Sumathi)
Professor and University Head
Interfaculty Department of Biochemistry,
_________________
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030

Member : (Dr. M.Yakadri)
Principal Scientist (Agro) & Head
Weed Science Research Centre
_________________
College of Agriculture, ANGRAU,
Rajendranagar, Hyderabad-500 030

Date of final viva-voce:
ACKNOWLEDGEMENTS
Mathru Devo Bavah
Pithru Devo Bavah
Acharya Devo Bavah
First and foremost, I offer my obeisance to the ‘Almighty’ for his boundless
blessing, which accompanied me in all the endeavors.
I am pleased to place my profound etiquette to Dr. R. SUBHASH REDDY
Professor and University Head Department Of Agricultural Microbiology and
Bioenergy, College of Agriculture, Rajendranagar, Hyderabad and esteemed Chairman
of my Advisory Committee for his learned counsel, unstinted attention, arduous and
meticulous guidance on the work in all stages. His keen interest, patient hearing and
constructive criticism have installed in me the spirit of confidence to successfully
complete the task.
I sincerely extend my profound gratitude and appreciation to the member of my
advisory committee to Dr S. SUMATHI, Professor and University Head, Interfaculty
Department of Biochemistry, College of Agriculture, Rajendranagar, Hyderabad, for
her valuable help and cooperation during the course of my study.
I deem it my privilege in expressing my fidelity to Dr. M.YAKADRI Senior
Scientist (Agro) & Head Weed Science Research Centre, College of Agriculture,
Rajendranagar, Hyderabad and member of my Advisory Committee for his munificent
acquiescence and meticulous reasoning to refine this thesis and most explicitly to
reckon with set standards. Ineffable in my gratitude and sincere thanks to him for his
transcendent suggestions and efforts to embellish the study.
I sincerely extend my profound gratitude and appreciation to Mrs. Reshma
karan, Mrs. Srilatha Agricultural Officer, Ms. Jayamma, Ms. Bindu, Mrs. Sridevi, Mr.
Jaffer, Mr. Venkataiah and workers Mrs. Salamma, Department of Agricultural
Microbiology and Bioenergy, College of Agriculture, Rajendranagar, Hyderabad.
Words are not enough to express my whole-hearted and affectionate gratitude to
my beloved parents Sri. Raghuram Mehta and Smt. Champi Mehta, uncle Sri.
Laxminarayan Mehta and my loving aunt Smt. Ganga Mehta, elder brother Manoj
Mehta and my loving bhabhi Smt.Gaytri Mehta, ever loving brother Rajesh, Hemant
and sisters Hemlata, Kalawati. Vidhya and my nephew Chanchal, Mahima, Harshita
and all my family members and relatives for their unbounding love, unparallel affection
and unstinted encouragement throughout my educational career and without whose
invaluable moral support, the thesis would not have seen the light of the day.
I am greatly beholden and owe a deep sense of honor to my ever loving wife
Rekha for her caring in every aspect either studies or personal life,co-operation,
unparalleled affection, moral support and persistant encouragement of her help to
accomplish my research work successfully.
I express my heartfelt gratitude and thanks to my classmates, Tirupati Rao,
Ganesh, my seniors, Revathi, Supraja, Shanthi, Madhavi, Satish, Sharvani, Naveen,
Manoj and Lakshmi Narayana, my juniors, Sai prasad, Rajshekar, Harikrishna,
Naveen, Rani and Tejashree for their valuable guidance and encouragement.
No words are enough to express the affection to my best friends, Ravi, Manoj,
Kailash, Kenny, Rahul, Suggana, Deepak, Soodarshan, Sonali, Mooni, Pavan,
Suvendhu, Meena, Bhim, Nitesh, Hemant, Vijay for their help, guidance, constant
encouragement and companionship in my personal and professional life and for their
moral support during research work.
I humbly thank the authorities of Indian Council of Agricultural Research,
Acharya N.G. Ranga Agricultural University and Government of Andhra Pradesh for
the financial help in the form of stipend during my study period.
Finally, I wish my humble thanks to one and all who have directly or indirectly
contributed to the conduct of the study.

(HARISH KUMAR MEHTA)
LIST OF CONTENTS

Chapter No. Title Page No.
I INTRODUCTION
II REVIEW OF LITERATURE
III MATERIAL AND METHODS
IV RESULTS AND DISCUSSION
V SUMMARY AND CONCLUSIONS
LITERATURE CITED
APPENDICES
LIST OF TABLES

Table Page
Title
No. No.
3.1 Details of different vermicomposts used in the study.
3.2 Details of treatments used in the enriched vermicompost analysis.
3.3 Physico-chemical properties of soil in the experimental site.
3.4 Microbial population in the soil before sowing of crop.
3.5 Details of treatments used in the field experiment.
Viable population of Bacteria, Moulds, Actinomycetes, Rhizobia and
4.1
Pseudomonas in different vermicomposts.
4.2 Chemical properties of different vermicomposts.
Change in bacterial population in the vermicompost enriched with
4.3
biofertilizers.
Change in population of fungi in the vermicompost enriched with
4.4
biofertilizers.
Change in population of actinomycetes in the vermicompost enriched
4.5
with biofertilizers.
Change in population of Rhizobia in the vermicompost enriched with
4.6
biofertilizers.
Change in population of Pseudomonas in the vermicompost enriched
4.7
with biofertilizers.
Available nitrogen content in the vermicompost enriched with
4.8
biofertilizers.
Available phosphorus content in the vermicompost enriched with
4.9
biofertilizers.
Available potassium content in the vermicompost enriched with
4.10
biofertilizers.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.11
the plant height and root length in black gram.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.12
the LAI and chlorophyll content (SPAD value) in black gram.
Influence of biofertilizers, vermicompost and chemical fertilizers on
the number of nodules, nodule dry weight (peak flowering stage), dry
4.13
matter accumulation and seed protein content (harvest stage) in black
gram.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.14
the yield attributes and yield (kg ha-1) in black gram.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.15 the nitrogen, phosphorus and potassium uptake in black gram at harvest
stage.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.16 the available soil nitrogen, phosphorus and potassium after harvesting
of black gram.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.17 the soil microbial population (CFU g-1 soil) at harvest stage of black
gram.
Influence of biofertilizers, vermicompost and chemical fertilizers on
4.18
the economics of black gram.