Methods in Protein Sequence Analysis: Proceedings of the 7th International Conference, Berlin, July 3–8, 1988

607 Pages·1989·16.496 MB·English

by  P. E. Hare (auth.), Professor Dr. Brigitte Wittmann-Liebold (eds.)

Brigitte Wittmann-Liebold (Ed.)
Methods in Protein
Sequence Analysis
Proceedings of the 7th International Conference,
Berlin, July 3-8, 1988
With 201 Figures
Springer-Verlag
Berlin Heidelberg New York
London Paris Tokyo
Professor Dr. BRIGITI'E WlTIMANN-L!EBOLD
Max-Planck-Institut fUr Molekulare Genetik
Abteilung Wittmann
Ihnestrasse 73
0-1000 Berlin 33 (Dahlem)
ISBN-13:978-3-642-73836-4 e-ISBN-13:978-3-642-73834-0
DOl: 10.1007/978-3-642-73834-0
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© Springer-Verlag Berlin Heidelberg 1989
Softcover reprint of the hardcover 1st edition 1989
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213113130-543210 - Printed on acid-free paper
Preface
"Methods in Protein Sequence Analysis - 1988" - contains
selected contributions on modern protein- analytical techniques
as presented by speakers at the Seventh International Conference
on "Methods in Protein Sequence Analysis", held from July 3rd to
July 8th, 1988 in Berlin.
The book contains information on new methodologies for
sensitive amino acid analysis, N- and C-terminal sequence
analysis, and protein and peptide purification. In addition
recent mass spectrometric approaches are described, as an alter
native technique to the common stepwise degradative sequence
analysis of polypeptides by the Edman method. The book presents
new possibilities in the design of sequencers and sophisticated
equipment for the structural analysis of peptides and proteins.
It describes practical approaches for the investigation of
protein domains and protein complexes, and contains review
chapters on the crystallization of cell organelles as well as on
recent theoretical aspects of protein folding mechanisms. The
nature of protein folding is not yet understood, but further
advances in this area would greatly enhance our present knowledge
of protein structure and function. Further, the book gives
examples of the application of gene technology to protein
characterization and to the design of new proteins. This enables
new studies on the structure and function of proteins to be made,
and opens up efficient approaches to the design of drugs.
A conference on modern methods in protein chemistry has
to cover a broad spectrum of current techniques in this field. In
consequence, not all of the methods can be comprehensively
covered, but rather highlights in the development of new
VI
procedures and instrumentation were selected. The interest in
this conference was overwhelming. and this was reflected by the
attendance of more than 500 participants. This demonstrates that
the remarkable progress in the field of molecular biology is
being paralled by a rapidly growing worldwide interest in methods
for highly sensitive protein structural analysis.
The editor wishes to express special thanks to Professor
Volker A. Erdmann as treasurer of the conference. and to
Professor Ferdinand Hucho who supervised the poster session and
the exhibition on "Analysis and Synthesis of Biopolymers"
organized by Ch. Scheffen and H. Orschel from Catalyst. Berlin.
Furthermore. the editor gratefully acknowledges the helpful
suggestions of the various members of the organizing committee in
putting together the scientific programme. and is indebted to
Hannelore Erdmann for her valuable help in the organization of
the conference. and to Inge Bauer. Maryse Kopke. Julie Ludwig and
uta Mertingk for secretarial work.
October. 1988 Brigitte Tittmann-Liebold
Committee Members
P. ARGOS, Heidelberg, FRG
M.Z. ATASSI, Houston, TX, U.S.A.
K. BIEUANN, Cambridge, MA, U.S.A.
A.S. BHOWN, Birmingham, AL, U.S.A.
W. BRANDT, CapeTown, SA
G. BUSE, Aachen, FRG
J.D. CAPRA, Dallas, TX, U.S.A.
J.Y. CHANG, Basel, Switzerland
M.-A. COLETTI-PREVIERO, Montpellier, France
G. ETZOLD, Berlin, DDR
M. ELZINGA, Brookhaven, NY, U.S.A.
I. FONTANA, Padua, Italy
G. FRANK, Zurich, Switzerland
R.W. FRANK, Heidelberg, FRG
A. HENSCHEN, Munchen, FRG
M.A. HERMODSON, W. Lafayette, IN, U.S.A.
L.E. HOOD, Pasadena, CA, U.S.A.
F. HUCHO, Berlin, FRG
M.W. HUNKAPILLER, Foster City, CA, U.S.A.
K. IMAHORI, Tokyo, Japan
A.S. INGLIS, Parkville, Victoria, Australia
H. J6RNVALL, Stockholm, Sweden
P. JOLLtS, Paris, France
R.A. LAURSEN, Boston, MA, U.S.A.
W. KACHLEIDT, Munchen, FRG
B. MELOUN, Praha, CSSR
E. MtNnEZ, Madrid, Spain
I. MOLNAR, Berlin, FRG
K. MURAMOTO, Iwate, Japan
H. NEURATH, Seattle, WA, U.S.A.
K. NAGANO, Tokyo, Japan
Y.A. OVCHINNIKOV, Moscow, USSR
R.N. PERHAM, Cambridge, UK
H. PONSTINGL, Heidelberg, FRG
L. PHILIPSON, Heidelberg, FRG
Y. SHIMONISHI, Osaka, Japan
J. SPIESS, San Diego, CA, U.S.A./ Gottingen, FRG
H. TSCHESCHE, Bielefeld, FRG
K.A. WALSH, Seattle, WA, U.S.A.
H.G. WITTMANN, Berlin, FRG
J. VANDEKERCKHOVE, Gent, Belgium
C.Y. YANG, Houston, TX, U.S.A.
A. YONATH, Rehovot, Israel/ Hamburg, FRG
Acknowledgements
The organization committee greatly appreciates the support of
the Seventh International Conference on "Methods in Protein
Sequence Analysis" by the following societies and companies:
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V.
SENAT DER STADT BERLIN
FREIE UNIVERSITAT BERLIN
APPLIED BIOSYSTEMS LTD.
Foster City. California
Dr. ING. HERBERT KNAUER GMBH
Wissenschaftliche Geratebau. Berlin-Zehlendorf
E. MERCK
Biochemica. Darmstadt
SCHERING AKTIENGESELLSCHAFT
Berlin-Wedding
BOEHRINGER MANNHEIM GMBH
Mannheim
HOECHST AG
Pharma Forschung Biochemie. Frankfurt
BAYER AG
Institut fur Biochemie. Wuppertal
Parallel to the Conference the following Instrument Exhibition
and Demonstration was organized:
"ANALYSIS AND SYNTHESIS OF BIOPOLYMERS"
which was supported by the following companies:
ABIMED Analysen-Technik. Langenfeld
AMERSHAM BUCHLER. Braunschweig
APPLIED BIOSYSTEMS LTD .. Foster City. CA. U.S.A.
APPLIED PROTEIN TECHNOLOGIES.- INC.
Cambridge. MA. U.S.A.
x
BECKMAN INSTRUMENTS GmbH. Division Bioanalytik Munchen
BIO.ION NORDIC AB. Uppsala. Sweden
BIOTECHNOLOGY COMPUTER SYSTEMS LTD .. London. U.K.
CIBA CORNING. Sudbury. Suffolk. U.K.
ERC
Gesellschaft fur den Vertrieb Wissenschaftlicher Gerate
Alteglofsheim/ Regensburg
Far.italia Carlo Erba
R. S. Biotecnologie/Chimica dei Peptidi. Milano. Italy
H. HOLZEL GMBH. Dorfen/Munchen
JAYTEE BIOSCIENCES LTD .. Kent. U.K.
KONTRON INSTRUMENTS GMBH. Munchen
LANGE & SPRINGER. Berlin
LATEK
Labortechnik-Gerate GmbH & Co .. Eppelheim
E. MERCK. Biochemica. Darmstadt
MILLIGEN. Division of Millipore. Bedford. MA. U.S.A.
Dr. I. MOLNAR. Berlin
M-SCAN. Ascot. U.K.
SHIKADZU EUROPA GMBH. Duisburg
VYDAC
The Separation Group. Hesperia. California. U.S.A.
VG INSTRUMENTS GMBH. Wiesbaden
Contents
Chapter 1
A Critical Evaluation of Sensitive Amino Acid Analysis
1.1 Detection Limits in Amino Acid Analysis: An Overview
P.E. HARE .......................................... . 2
1.2 Amino Acid Analysis at High Sensitivity: A Critical
Review of the Communications
G.J. HUGHES and S. FRUTIGER......................... 10
1.3 A Comparison of 9-Fluorenylmethylchloroformate
(FMOC-Cl) Amino Acid Analysis with Ninhydrin and
Phenylthiocarbamyl (PTC) Chemistries
ALAN J. SMITH. J.M. PRESLEY and W. McINTIRE......... 14
1.4 Localization of Endogenous Phosphoserine Residues in
the Primary Structure of Proteins
H.E. MEYER. C.C. KUHN. G.F. MEYER. K. SWIDEREK.
C. WEBER. E. HOFFMANN-POSORSKE. H. KORTE and
L . M. G. HE I LMEYER . Jr................................ 19
1.5 Quantitative N-terminal Analysis of Polypeptides
J . Y. CHANG.......................................... 23
Chapter 2
New Approaches for the Chemistry of the Edman Degradation
2.1 Study on New Edman-type Reagents
S.-W. JIN. S.-Z. XU. X.-L. ZHANG ~nd T.-B. TANG..... 34
XII
2.2 Protein Microsequence Analysis with Dansylamino-PITC
H. HIRANO and B. WITTMANN-LIEBOLD................... 42
2.3 Manual Gas Phase Isothiocyanate Degradation
W.F. BRANDT and G. FRANK............................ 52
Chapter 3
New Instrumentation in Sequence Analysis and Synthesis of
Biopolymers
3.1 A Second Generation Solid-phase Protein Sequencer:
The Prosequencer™
R.A. LAURSEN, J.D. DIXON, S.-P. LIANG, D.M. NGUYEN,
T. KELCOURSE, L. UDELL and D. PAPPIN................ 61
3.2 Development of Membrane Supports for the Solid-phase
Sequence Analysis of Proteins and Peptides
J.M. COULL, J.D. DIXON, R.A. LAURSEN. H. KOSTER and
D. PAPPIN........................................... 69
3.3 Accelerated High Sensitivity Sequence Analysis of
Proteins and Peptides Immobilized on Chemically
modified Glass Fiber Discs
R.H. AEBERSOLD, H. NIKA, G.D. PIPES, R.E.H. WETTENHALL
S.M. CLARK, L.E. HOOD and S.B.H. KENT............... 79
3.4 A New Modular Sequencer
STEFAN FISCHER, F. REIMANN and B. WITTMANN-LIEBOLD.. 98
3.5 An Improved Injection System for On-line High
Sensitivity Phenylthiohydantoin Amino Acid Analysis
G.S. BEGG and R.J. SIMPSON.......................... 108
3.6 Design and Performance Features of a Simplified Protein
Sequencer with an On-Board Isocratic HPLC System
R.J. MATTALIANO, K.A. YAMADA, J.K. HUGHES and
P.-M. YUAN.......................................... 112