Table Of ContentM E T H O D S I N P H A R M A C O L O G Y A N D T O X I C O L O G Y
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Methods in Biological Oxidative Stress
METHODS IN PHARMACOLOGY AND TOXICOLOGY
Mannfred A. Hollinger, PhD SERIES EDITOR
Methods in Biological Oxidative Stress
edited by Kenneth Hensley and Robert A. Floyd, 2003
Apoptosis Methods in Pharmacology and Toxicology: Approaches to
Measurement and Quantification
edited by Myrtle A. Davis, 2002
Ion Channel Localization: Methods and Protocols
edited by Anatoli N. Lopatin and Colin G. Nichols, 2001
METHODS IN PHARMACOLOGY AND TOXICOLOGY
Methods in Biological
Oxidative Stress
Edited by
Kenneth Hensley
Robert A. Floyd
Free Radical Biology and Aging Research Program
Oklahoma Medical Research Foundation
Oklahoma City, OK
Humana Press Totowa, New Jersey
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Library of Congress Cataloging-in-Publication Data
Methods in biological oxidative stress / edited by Kenneth Hensley, Robert A. Floyd.
p. cm.
Includes bibliographical references and index.
ISBN 0-89603-815-7 (alk. paper); E-ISBN 1-59259-424-7
1 . Active oxygen in the body--Laboratory manuals. 2. Oxidation, Physiological--Laboratory
manuals. 3. Stress (Physiology)--Laboratory manuals. I. Hensley, Kenneth. II. Floyd, Robert A.,
1940-
RB170 .M48 2003
616.07--dc21
2002033397
Foreword
Oxidative damage appears to play a central role in the development of
a wide range of tissue pathology, including neurodegenerative disease, drug
side-effects, xenobiotic toxicity, carcinogenesis, and the aging process,
to name just a few.
Because of the centrality of oxidative processes to normal and abnormal
tissue function, it has become imperative to develop appropriate analytical
techniques to facilitate the quantitation of significant reactants. Without
advances in methodology, corresponding advances in our knowledge of
underlying biochemical events will be necessarily limited.
Drs. Hensley and Floyd have done an outstanding job of assembling the
work of world-class experts into Methods in Biological Oxidative Stress.
The contributors have presented concise, yet thorough, descriptions of the
state-of-the-art methods that any investigator working in the field needs to
access.
Mannfred A. Hollinger
v
Preface
Free radicals and reactive oxidizing agents were once ignored as
biochemical entities not worth close scrutiny, but are now recognized as
causes or contributing factors in dozens, if not hundreds, of disease states. In
addition, free radical metabolisms of xenobiotics have become increasingly
important to pharmacologists. Accordingly, the need has arisen to accurately
quantify reactive oxygen species and their byproducts.
Methods in Biological Oxidative Stress is practical in scope, providing
the details of up-to-date techniques for measuring oxidative stress and
detecting oxidizing agents both in vitro and in vivo. The contributors are
recognized experts in the field of oxidative stress who have developed novel
strategies for studying biological oxidations.
The chapters of Methods in Biological Oxidative Stress cover widely used
standard laboratory techniques, often developed by the authors, as well as
HPLC–electrochemical measurement of protein oxidation products, particularly
nitrotyrosine and dityrosine, and HPLC–electrochemical detection of DNA
oxidation products. Additionally, recently developed techniques are
presented to measure lipid oxidation and nitration products such as 5-NO -
2
γ-tocopherol and isoprostanes, using HPLC-electrochemical/photodiode
array methods and mass spectrometry as well as electron paramagnetic
resonance (EPR) techniques.
In scope, presentation, and authority therefore, Methods in Biological
Oxidative Stress was designed to be an invaluable manual for clinical
laboratories and teaching institutions now conducting routine measurements
of biological oxidants and biological oxidative stress or implementing new
programs in this vital area of research. As a reference work, this collection
of techniques and methods will prove useful for many years to come.
Kenneth Hensley
Robert A. Floyd
vii
Contents
Foreword.............................................................................................v
Preface.............................................................................................. vii
Contributors...................................................................................xiii
PART I LIPIDS
1 Measurement of Fat-Soluble Vitamins and Antioxidants
by HPLC With Electrochemical Array Detection
Paul H. Gamache, Paul A. Ullucci, Joe A. Archangelo,
and Ian N. Acworth...................................................................... 3
2 Analysis of Aldehydic Markers of Lipid Peroxidation in
Biological Tissues by HPLC With Fluorescence Detection
Mark A. Lovell and William R. Markesbery............................. 17
3 Measurement of Isofurans by Gas Chromatography–
Mass Spectrometry/Negative Ion Chemical Ionization
Joshua P. Fessel and L. Jackson Roberts, II............................... 23
4 Analysis of F -Isoprostanes by Gas Chromatography–Mass
2
Spectrometry/Negative Ion Chemical Ionization
L. Jackson Roberts, II and Jason D. Morrow............................. 33
5 Measurement of F -Neuroprostanes by Gas Chromatography–
4
Mass Spectrometry/Negative Ion Chemical Ionization
Nathalie Bernoud-Hubac and L. Jackson Roberts, II .............. 41
6 Immunoassays for Lipid Peroxidation End Products:
One-Hour ELISA for Protein-Bound Acrolein and HNE
Kimihiko Satoh and Koji Uchida ................................................ 49
7 Fluorometric and Colorimetric Assessment of Thiobarbituric
Acid-Reactive Lipid Aldehydes in Biological Matrices
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd.... 57
8 HPLC With Electrochemical and Photodiode Array
Detection Analysis of Tocopherol Oxidation
and Nitration Products in Human Plasma
Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd.... 67
ix
