Table Of ContentContributors to Volume 226
elcitrA srebmun era ni sesehtnerap gniwollof the seman of .srotubirtnoc
snoitailiffA listed era .tnerruc
DAVID S. AULD (23), Center for Biochem- TERESA B. FREEDMAN (13, 19), Department
ical and Biophysical Sciences and Medi- of Chemistry, Syracuse University, 410-1
cine, Harvard Medical School, Boston, Center for Science and Technology, Syra-
Massachusetts 51120 cuse, New York 44231
JANINA C. AUSTIN (15), Department of SIMON J. GEORGE (9), Department of Ap-
Chemistry, Princeton University, Prince- plied Science, University of California,
ton, New Jersey 44580 Davis, Davis, California 61659
BRUCE A. AVERILL (2), Department of ROBERT A. GOLDBECK (7), Department of
Chemistry, University of Virginia, Chemistry and Biochemistry, University
Charlottesville, Virginia 10922 of California, Santa Cruz, Santa Cruz,
GERALD T. BABCOCK (17), The LASER Lab- California 46059
oratory, and Department of Chemistry, WILLIAM DEW. HORROCKS, JR. (21), De-
Michigan State University, East Lansing, partment of Chemistry, The Pennsylvania
Michigan 42884 State University, University Park, Penn-
JAcQUELINE K. BARTON (25), Division of sylvania 20861
Chemistry and Chemical Engineering, DAVID S. KLIGER (7), Department of Chem-
,03-461 California Institute of Technol- istry and Biochemistry, University of -laC
ogy, Pasadena, California 52119 ifornia, Santa Cruz, Santa Cruz, Califor-
MARK R. CHANCE (5, 6), Department of ain 46059
Physiology and Biophysics, Albert Ein- NENAD M. KOSTI~ (24), Department of
stein College of Medicine, Bronx, New Chemistry, Iowa State University, Ames,
York 16401 Iowa 11005
MYLES R. CHEESMAN (9), Center for Metal- Louis B. LACROIX (1), Department of
loprotein Spectroscopy and Biology, Chemistry, Stanford University, Stan-
School of Chemical Sciences, University ford, California 50349
of East Anglia, Norwich NR4 7T J, United JAMES A. LARRABEE (12), Department of
Kingdom Chemistry and Biochemistry, Science
EEFEI CHEN (6), Department of Chemistry, Center, Middlebury College, Middlebury,
University of California, Santa Cruz, Vermont 35750
Santa Cruz, California 46059 THOMAS M. LOEHR (18), Department of
SUNHEE CHOI (12), Department of Chemis- Chemical and Biological Sciences, Ore-
yrt and Biochemistry, Science Center, gon Graduate Institute of Science and
Middlebury College, Middlebury, Ver- Technology, Beaverton, Oregon 60079
mont 35750 MICHAEL D. LOWERY (1), Department of
ROMAN S. CZERNUSZEWICZ (11), Depart- Chemistry, Stanford University, Stan..
ment of Chemistry, University of Hous- ford, California 50349
ton, Houston, Texas 40277 WOLFGANG MARET (3, 26), Center for Bio-
ROBERT C. DUNN (8), Pacific Northwest chemical and Biophysical Sciences and
Laboratories, Battelle, Richland, Wash- Medicine, Harvard Medical School, Bos-
ington 25399 ton, Massachusetts 511'20
vii
Vlll CONTRIBUTORS TO VOLUME 226
CATHERINE J. MURPHY (25), Division of GIULIETTA SMULEVICH (16), Laboratorio di
Chemistry and Chemical Engineering, Spettroscopia Molecolare, Dipartimento
,03-461 California Institute of Technol- di Chimica, Universita di Firenze, 12105
ogy, Pasadena, California 52119 Florence, Italy
EDWARD I. SOLOMON (1), Department of
LAURENCE A. NAFIE 03, 19), Department
Chemistry, Stanford University, Stan-
of Chemistry, Syracuse University, 410-1
ford, California 50349
Center for Science and Technology, Syra-
cuse, New York 44231 THOMAS G. SPIRO (15, 16), Department of
Chemistry, Princeton University, Prince-
KAZUO NAKAMOTO (11), Department of ton, New Jersey 44580
Chemistry, Marquette University, Mil-
ANDREW J. THOMSON (9), Center for Metal-
waukee, Wisconsin 33235
loprotein Spectroscopy and Biology,
THOMAS V. O'HALLORAN (4), Department School of Chemical Sciences, University
of Chemistry, Northwestern University, of East Anglia, Norwich NR 4 7TJ,
Evanston, Illinois 80206 United Kingdom
LISA M. UTSCHIG (4), Department of Chem-
FREDERICK S. RICHARDSON (22), Depart-
istry, Northwestern University, Evanston,
ment of Chemistry, University of Vir-
Illinois 80206
ginia, Charlottesville, Virginia 10922
BERT L. VALLEE (3), Center for Biochem-
JAMES P. RIEHL (22), Department of Chem- ical and Biophysical Sciences and Medi-
istry, University of Missouri, St. Louis, cine, Harvard Medical School, Boston,
St. Louis, Missouri 12136 Massachusetts 51120
ALISON RODGER (10), Physical Chemistry HAROLD E. VAN WART (14), Institute of
Biochemistry and Cell Biology, Syntex
Laboratory, Oxford University, Oxford
Discovery Research, Paid Alto, Califor-
1(5.0 3QZ, England
nia 30349
KENTON R. ROGERS (15), Department of SONITNATSNOC VAROTSIS (17), ehT LASER
Chemistry, Princeton University, Prince-
Laboratory, and Department of Chemis-
ton, New Jersey 44580 try, Michigan State University, East
DAVID E. ROOT (1), Department of Chemis- Lansing, Michigan 42884
try, Stanford University, Stanford. Cali- JOHN B. VINCENT (2), Department of
fornia 50349 Chemistry, University of Alabama, Tus-
caloosa, Alabama 78453
JOANN SANDERS-LOEHR (18), Department
YANG WANG (14), George R. Harrison,
of Chemical and Biological Sciences, -rO
Spectroscopy Laboratory, Massachusetts
egon Graduate Institute of Science and
Institute of Technology, Cambridge,
Technology, Beaverton, Oregon 60079
Massachusetts 93120
JOHN D. SIMON (8), Department of Chemis- JEFFREY G. WRIGHT (4), Department of
try, University of California, San Diego, Chemistry, Northwestern University,
La Jolla, California 39029 Evanston, Illinois 80206
W. E. SMITH (20), Department of Pure and XIAOLIANG XIE (8), Pacific Northwest Lab-
Applied Chemistry, University of Strath- oratories, Battelle, Richland, Washington
clyde, Glasgow 1G IXL, Scotland 25399
Preface
The scope of metallobiochemistry has greatly expanded in recent
years as ever more powerful techniques have been brought to bear on the
constituent elements that define and characterize the subject. Metallobio-
chemistry, Part A, Volume 851 of Methods in Enzymology, focused on
progress in those areas in which early on there were major impediments to
growth and development. Without the tools to measure metals with suffi-
cient accuracy, precision, and sensitivity this scientific discipline could
not have existed. In addition, unless it were possible to distinguish be-
tween the metals present in a biological sample that belonged there (be-
cause nature intended them to be) from those that merely appeared by
accident (adventitious contamination), such metal analyses would have
been meaningless. Technology overcame these hurdles, and Part A de-
tailed the state of trace element analysis and the various approaches
employed by the metallobiochemist to avoid artifacts and achieve the
inorganic equivalent of microbiological sterility. It is a summary of the
critical methods that have helped place the subject on a solid foundation.
Metallobiochemistry, Part B, Volume 205 of this series, is devoted to
metallothionein and related molecules. It is unusual for a Methods in
Enzymology volume to feature a single molecule, but the surge of interest
in metallothionein and its structural and possibly functional relationship
to DNA-binding proteins suggested that such a volume would be timely
and useful. It also seemed appropriate to stress that not all metal-contain-
ing biological molecules are metalloenzymes or electron-transport pro-
teins.
Parts C and D, Volumes 226 and 227, respectively, return to the theme
of methods that have contributed to and are emerging as important factors
in the advancement of the field. These methods embody concepts that had
their origin about the time of the almost forgotten Sumner-Willstfitter
controversy of the 1920s. Proteins, it was claimed, could hardly serve as
specific biological catalysts if they were little more than nondescript col-
loids. Metal ions would prove to be the real actors on the enzymatic stage.
Authority prevailed until the crystallization of urease seemingly dis-
patched the metal dogma to oblivion. Despite the extended protests of
interest-vested diehards, protein chemistry became inextricably associ-
ated with enzymology and metals fell out of fashion. (Ironically, urease
turned out to be a nickel enzyme.)
Biochemists who had witnessed this metal-induced brouhaha were
understandably reluctant to resurrect the idea that metals might have
something to do with biological catalysis. Anyone wishing to make the
case would have to have persistence along with persuasive and unassail-
ix
X ECAFERP
able analytical data. Only through scrupulous attention to detail was it
possible in those neonatal days of metallobiochemistry to gain the accep-
tance that allowed the field to grow and flourish.
Despite its shortcomings the metal-cum-colloid view of catalysis did
have one rather appealing feature: the metal would have unique proper-
ties among all the atoms of the protein and perhaps these could be ex-
ploited to gain important information. The metal could serve as a beacon
to guide the investigator searching for an active site. It could also be a
signal, either of the detailed steps of catalysis or of any other biological
function with which the metal might be associated. Emission spectros-
copy proved the significance of a metal-derived signal in principle, but
was rather inconsiderate of the protein. Hence, attention shifted along
with wavelength to absorption spectroscopy whereby it became possible
to view the functional heart of a metalloenzyme directly. This window on
the world of metallobiochemistry revealed unprecedented spectral fea-
tures clearly indicative of an unusual coordination environment and likely
characteristic of a catalytic site.
Not all metals lend themselves to absorption spectroscopic investiga-
tion. Zinc, one of nature's most recurrent participants, is notoriously shy
in this regard. Other metals are more expressive and revealing when
viewed by alternative techniques. In these two volumes (226 and 227) we
have assembled a broad representation of the physical and spectroscopic
methods now available that can be useful for examining metals in biologi-
cal systems and for probing their environments in metalloproteins and
metalloenzymes. These approaches, while by no means all-inclusive, ex-
emplify the wide variety of tools and the level of sophistication currently
being applied to extract both the nuances and the general principles of
metallobiochemistry pertinent to these systems.
We are extremely grateful to our contributors for their willingness to
participate in this endeavor. They have made a concerted effort to de-
scribe techniques in ways that would be most beneficial to the reader. The
chapters differ from the more typical ones in this series in that they
identify principles underlying a particular method, the kinds of questions
that can be addressed, and the ways to interpret results. Step-by-step
instructions were not practical in most cases, and generally the objective
has been to provide a sense of what can be accomplished. It required
more description than anticipated for most of the topics, and this necessi-
tated two volumes instead of one. We appreciate the understanding of our
colleagues at Academic Press and we thank them again as well as all the
contributors for making this such a pleasant experience.
JAMES F. RIORDAN
BERT L. VALLEE
METHODS IN ENZYMOLOGY
EMULOV I. Preparation and Assay of Enzymes
Edited yb SIDNEY P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV II. Preparation and Assay of Enzymes
Edited yb SIDNEY P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV III. Preparation and Assay of Substrates
Edited yb SIDNEY P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV IV. Special Techniques for the Enzymologist
Edited yb YENDIS P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV V. Preparation and Assay of Enzymes
Edited yb SIDNEY P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
Edited yb SIDNEY P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VII. Cumulative Subject Index
Edited yb SIDNEY P. KCIWOLOC DNA NAHTAN O. NALPAK
EMULOV VIII. Complex Carbohydrates
Edited yb HTEBAZILE F. NEUFELD DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb WILLIS A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited yb DLANOR W. KOORBATSE DNA DRANYAM E. NAMLLUP
EMULOV XI. Enzyme Structure
Edited yb C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited yb HTENNEK NITSUK
ix
xii
METHODS IN ENZYMOLOGY
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DLANOD B. McCoRMICK DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited yb EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part )C
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb WILLtAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited yb ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited yb SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited yb AHTRAM G. DENNIS DNA DRAWDE a. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part B)
Edited yb JOHN M. NIETSNEWOL
METHODS IN ENZYMOLOGY xiii
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited yb BERT W. O'MALLEY AND JOEL G. HARDMAN
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. HARDMAN AND BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and Organ
Systems)
Edited by JOEL G. HARDMAN AND BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited yb KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited yb OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited yb WILLIAM B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited yb C. H. W. HIRS DNA EGRES N. EEEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HtRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part )C
Edited by ROTCIV GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited yb PATRICIA A. HOFFEE AND MARY ELLEN JONES
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited yb SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited yb SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited yb SIDNEY FLEISCHER AND LESTER PACKER
xiv SDOHTEM IN YGOLOMYZNE
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited yb SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited yb SIDNEY REHCSIELF DNA RETSEL REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited yb ENELRAM A. DELUCA
EMULOV LVIII. Cell Culture
Edited yb WILLIAM B. YBOKAJ DNA ARI NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited yb KIVIE MOLDAVE DNA LAWRENCE NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited yb KIVIE MOEDAVE DNA LAWRENCE NAMSSORG
EMULOV 61. Enzyme Structure (Part H)
Edited yb C. H. W. HIRS DNA EGRES N. EEEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited yb DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
EMULOV 63. Enzyme Kinetics and Mechanism (Part A: Initial Rate and Inhibi-
tor Methods)
Edited by DANIEL L. PURICH
EMULOV .46 Enzyme Kinetics and Mechanism (Part B: Isotopic Probes and
Complex Enzyme Systems)
Edited yb DANIEL L. PURICH
EMULOV .56 Nucleic Acids (Part I)
Edited by LAWRENCE NAMSSORG DNA KIVIE MOLDAVE
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited yb DONALD B. McCoRMICK DNA LEMUEL D. WRIGHT
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited yb DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
VOLUME 68. Recombinant DNA
Edited yb YAR Wu
EMULOV 69. Photosynthesis and Nitrogen Fixation (Part C)
Edited yb YNOHTNA NAS ORTEIP
EMULOV 70. Immunochemical Techniques (Part A)
Edited yb HELEN VAN VUNAKIS DNA NHOJ J. LANGONE
EMULOV 71. Lipids (Part C)
Edited yb JOHN M. NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited yb JOHN M. NIETSNEWOL
EMULOV 73. Immunochemical Techniques (Part )B
Edited yb JOHN J. LANGONE DNA HELEN VAN VUNAKIS
SDOHTEM IN YGOLOMYZNE VX
EMULOV 74. Immunochemical Techniques (Part C)
Edited by JOHN J. LANGONE DNA HELEN NAV SIKANUV
EMULOV 75. Cumulative Subject Index Volumes XXXI, XXXII, XXXIV-LX
Edited yb EDWARD A. DENNIS DNA MARTHA G. DENNIS
EMULOV 76. Hemoglobins
Edited by ERALDO ANTONINI, LUIGI ROSSI-BERNARDI, DNA EMILIA CHIAN-
ENOC
EMULOV 77. Detoxication and Drug Metabolism
Edited yb WILLIAM B. JAKOaY
EMULOV 78. Interferons (Part A)
Edited yb SIDNEY AKTSEP
EMULOV 79. Interferons (Part B)
Edited yb SIDNEY AKTSEP
EMULOV 80. Proteolytic Enzymes (Part C)
Edited yb LASZLO DNAROL
EMULOV .18 Biomembranes (Part H: Visual Pigments and Purple Mem-
branes, I)
Edited by LESTER REKCAP
EMULOV 82. Structural and Contractile Proteins (Part A: ExtraceUular Matrix)
Edited by LEON W. CUNNINGHAM DNA DIXIE W. FREDERIKSEN
EMULOV 83. Complex Carbohydrates (Part D)
Edited yb VICTOR GRUBSNIG
EMULOV 84. Immunochemical Techniques (Part D: Selected Immunoassays)
Edited yb JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV 85. Structural and Contractile Proteins (Part B: The Contractile Appa-
ratus and the Cytoskeleton)
Edited yb DIXIE W. FREDERIKSEN DNA LEON W. CUNNINGHAM
EMULOV 86. Prostaglandins and Arachidonate Metabolites
Edited yb WILLIAM E. M. LANDS DNA WILLIAM L. SMITH
EMULOV 87. Enzyme Kinetics and Mechanism (Part C: Intermediates, Stereo-
chemistry, and Rate Studies)
Edited by DANIEL L. PURICH
EMULOV .88 Biomembranes (Part I: Visual Pigments and Purple Mem-
branes, II)
Edited by LESTER REKCAP
EMULOV 89. Carbohydrate Metabolism (Part D)
Edited by WILLIS A. WOOD
EMULOV 90. Carbohydrate Metabolism (Part E)
Edited yb WILLIS A. WOOD
EMULOV 91. Enzyme Structure (Part I)
Edited by C. H. W. HIRS DNA SERGE N. EEEHSAMIT
xvi SDOHTEM NI YGOLOMYZNE
EMULOV .29 Immunochemical Techniques (Part E: Monoclonal Antibodies and
General Immunoassay Methods)
Edited yb JOHN J. ENOCNAL DNA HELEN NAV SIKANUV
EMULOV .39 Immunochemical Techniques (Part F: Conventional Antibodies,
Fc Receptors, and Cytotoxicity)
Edited by JOHN J. ENOGNAL DNA HELEN NAV SIKANUV
EMULOV 94. Polyamines
Edited yb TREBREH ROBAT DNA CELIA WHITE ROBAT
EMULOV .59 Cumulative Subject Index Volumes 61-74, 76-80
Edited yb DRAWDE A. DENNIS DNA AHTRAM G. SINNED
EMULOV .69 Biomembranes Part J: Membrane Biogenesis: Assembly and
Targeting (General Methods; Eukaryotes)
Edited yb YENDIS REHCSIELF DNA ACCEB REHCSIELF
EMULOV .79 Biomembranes Part K: Membrane Biogenesis: Assembly and
Targeting (Prokaryotes, Mitochondria, and Chloroplasts)
Edited by SIDNEY REHCSIELF DNA ACCEB REHCSIEEF
EMULOV 98. Biomembranes (Part L: Membrane Biogenesis: Processing and
Recycling)
Edited yb SIDNEY REHCSIELF DNA ACCEB REHCSIELF
EMULOV 99. Hormone Action (Part F: Protein Kinases)
Edited by EIKCAJ D. NIBROC DNA JOEL G. NAMDRAH
EMULOV 100. Recombinant DNA (Part B)
Edited yb YAR Wu, ECNERWAL ,NAMSSORG DNA KIVIE EVADEOM
EMULOV 101. Recombinant DNA (Part )C
Edited yb YAR Wu, ECNERWAL ,NAMSSORG DNA KIVIE EVADLOM
EMULOV 102. Hormone Action (Part G: Calmodulin and Calcium-Binding Pro-
teins)
Edited yb YNOHTNA R. SNAEM DNA BERT W. O'MALLEY
EMULOV 103. Hormone Action (Part H: Neuroendocrine Peptides)
Edited yb P. LEAHCIM NNOC
EMULOV 104. Enzyme Purification and Related Techniques (Part C)
Edited yb WILLIAM B. YBOKAJ
EMULOV 105. Oxygen Radicals in Biological Systems
Edited by RETSEL REKCAP
EMULOV 106. Posttranslational Modifications (Part A)
Edited yb FINN WOLD DNA KIVIE EVADLOM
EMULOV 107. Posttranslational Modifications (Part B)
Edited by FINN WOLD DNA KIVIE EVADEOM
EMULOV 108. Immunochemical Techniques (Part G: Separation and Character-
ization of Lymphoid Cells)
Edited yb INNAVOIG DI ,OTABAS JOHN J. ,ENOGNAL DNA
NELEH NAV SIKANUV
