Table Of ContentMET AL IONS IN
BIOLOGICAL SYSTEMS
Studies of Some Biochemical and
Environmental Problems
ADV ANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
Nathan Back Chairman, Department 0/ Biochemical Pharmacology, School 0/ Pharmacy,
State University of New York, Buffalo, New York
N. R. Di Luzio Chairman, Department 0/ Physiology,
Tulane University School 0/ Medicine, New Orleans, Louisiana
Alfred Gellhorn University 0/ Pennsylvania Medical School, Philadelphia, Pennsylvania
Bernard Halpern College de France,
Director of the Institute 0/ Immuno·Biology, Paris, France
Ephraim Katchalski Department of Biophysics, The Weizmann Institute of Science,
Rehovoth, Israel
David Kritchevsky Wistar Institute, Philadelphia, Pennsylvania
Abel Lajtha New York State Research Institute for Neurochemistry and Drug Addiction,
Ward's Island, New York
Rodolfo Paoletti Institute 0/ Pharmacology and Pharmacognosy,
University of Milan, Milan, Italy
Volume 1
THE RETICULOENDOTHELIAL SYSTEM AND ATHEROSCLEROSIS
Edited by N. R. Di Luzio and R. Paoletti' 1967
Volume 2
PHARMACOLOGY OF HORMONAL POLYPEPTIDES AND PROTEINS
Edited by N. Back, L. Martini, and R. Paoletti' 1968
Volume 3
GERM·FREE BIOLOGY: Experimental and Clinical Aspects
Edited by E. A. Mirand and N. Back' 1969
Volume 4
DRUGS AFFECTING LIPID METABOLISM
Edited by W. L. Holmes, L. A. Carlson, and R. Paoletti' 1969
VolumeS
LYMPHATIC TISSUE AND GERMINAL CENTERS IN IMMUNE RESPONSE
Edited by L. Fiore·Donati and M. G. Hanna, Jr. • 1969
Volume 6
RED CELL METABOLISM AND FUNCTION
Edited by George J. Brewer' 1970
Volume 7
SURFACE CHEMISTRY OF BIOLOGICAL SYSTEMS
Edited by Martin Blank' 1970
Volume 8
BRADYKININ AND RELATED KININS: Cardiovascular, Biochemical, and
Neural Actions
Edited by F. Sicuteri, M. Rocha e Silva, and N. Back' 1970
Volume 9
SHOCK: Biochemical, Pharmacological, and Clinical Aspects
Edited by A. Bertelli and N. Back' 1970
Volume 10
THE HUMAN TESTIS
Edited by E. Rosemberg and C. A. Paulsen' 1970
Volume 11
MUSCLE METABOLISM DURING EXERCISE
Edited by B. Pemow and B. Saltin • 1971
Volume 12
MORPHOLOGICAL AND FUNCTIONAL ASPECTS OF IMMUNITY
Edited by K. Lindahl·Kiessling, G. AIm, and M. G. Hanna, Jr. • 1971
Volume 13
CHEMISTRY AND BRAIN DEVELOPMENT
Edited by R. Paoletti and A. N. Davison' 1971
Volume 14
MEMBRANE·BOUND ENZYMES
Edited by G. Porcellati and F. di leso • 1971
Volume 15
THE RETICULOENDOTHELIAL SYSTEM AND IMMUNE PHENOMENA
Edited by N. R. Di Luzio and K. Flemming '1971
Volume 16A
THE ARTERY AND THE PROCESS OF ARTERIOSCLEROSIS: Pathogenesis
Edited by Stewart Wolf· 1971
Volume 16B
THE ARTERY AND THE PROCESS OF ARTERIOSCLEROSIS: Measurement and
Modification
Edited by Stewart Wolf, 1971
Volume 17
CONTROL OF RENIN SECRETION
Edited by Tatiana A. Assaykeen • 1972
Volume 18
THE DYNAMICS OF MERISTEM CELL POPULATIONS
Edited by Morton W. Miller and Charles C. Kuehnert· 1972
Volume 19
SPHINGOLIPIDS, SPHINGOLIPIDOSES AND ALLIED DISORDERS
Edited by Bruno W. Volk and Stanley M. Aronson' 1972
Volume 20
DRUG ABUSE: Nonmedical Use of Dependence·Producing Drugs
Edited by Simon Btesh • 1972
Volume 21
VA SOPEPTIDES: Chemistry, Pharmacology, and Pathophysiology
Edited by N. Back and F. Sicuteri • 1972
Volume 22
COMPARATIVE PATHOPHYSIOLOGY OF CIRCULATORY DISTURBANCES
Edited by Colin M. Bloor' 1972
Volume 23
THE FUNDAMENTAL MECHANISMS OF SHOCK
Edited by Lerner B. Hinshaw and Barbara G. Cox' 1972
Volume 24
THE VISUAL SYSTEM: Neurophysiology, Biophysics, and Their Clinical Applications
Edited by G. B. Arden' 1972
Volume 25
GLYCOLIPIDS, GLYCOPROTEINS, AND MUCOPOLYSACCHARIDES
OF THE NERVOUS SYSTEM
Edited by Vittorio Zambotti, Guido Tettamanti, and Mariagrazia Arrigoni' 1972
Volume 26
PHARMACOLOGICAL CONTROL OF LIPID METABOLISM
Edited by William L. Holmes, Rodolfo Paoletti,
and David Kritchevsky • 1972
Volume 27
DRUGS AND FETAL DEVELOPMENT
Edited by M. A. Klingberg, A. Abramovici, and.T. Chemke • 1973
Volume 28
HEMOGLOBIN AND RED CELL STRUCTURE AND FUNCTION
Edited by George l. Brewer' 1972
Volume 29
MICROENVIRONMENTAL ASPECTS OF IMMUNITY
Edited by Branislav D. Jankovic and Katarina Isakovic ·1972
Volume 30
HUMAN DEVELOPMENT AND THE THYROID GLAND: Relation to Endemic Cretinism
Edited by J. B. Stanbury and R. L. Kroc • 1972
Volume 31
IMMUNITY IN VIRAL AND RICKETTSIAL DISEASES
Edited by A. Kohn and M. A. Klingberg· 1973
Volume 32
FUNCTIONAL AND STRUCTURAL PROTEINS OF THE NERVOUS SYSTEM
Edited by A. N. Davison, P. Mandel, and I. G. Morgan· 1972
Volume 33
NEUROHUMORAL AND METABOLIC ASPECTS OF INJURY
Edited by A. G. B. Kovach, H. B. Stoner, and J. J. Spitzer· 1972
Volume 34
PLATELET FUNCTION AND THROMBOSIS: A Review of Methods
Edited by P. M. Mannucd and S. Gorini • 1972
Volume 35
ALCOHOL INTOXIC~TION AND WITHDRAWAL: Experimental Studies
Edited by Milton M. Gross· 1973
Volume 36
RECEPTORS FOR REPRODUCTIVE HORMONES
Edited by Bert W. O'Malley and Anthony R. Means· 1973
Volume 37A
OXYGEN TRANSPORT TO TISSUE: Instrumentation, Methods, and Physiology
Edited by Haim I. Bicher and Duane F. Bruley· 1973
Volume 37B
OXYGEN TRANSPORT TO TISSUE: Pharmacology, Mathematical Studies, and Neonatology
Edited by Duane F. Bruley and Haim I. Bieher • 1973
Volume 38
HUMAN HYPERLIPOPROTEINEMIAS: Principles and Methods
Edited by R. Fumagalli, G. Ricci, and S. Gorini • 1973
Volume 39
CURHENT TOPICS IN CORONARY HESEARCH
Edited by Colin M. Bloor and Ray A. Olsson· 1973
Volume 40
METAL IONS IN BIOLOGICAL SYSTEMS: Studies of Some Biochemical and Environmental Problems
Edited by Sanat K. Dhar • 1973
Volume 41A
PURINE METABOLISM IN MAN: Enzymes and Metabolic Pathways
Edited by O.Sperling, A. De Vries, and J. B. Wyngaarden • 1973
Volume 4lB
PURINE METABOLISM IN MAN: Biochemistry and Pharmacology of Uric Acid Metabolism
Edited by O. Sperling, A. De Vries, and J. B. Wyngaarden. 1973
METAL IONS IN
BIOLOGICAL SYSTEMS
Studies of Some Biochemical and
Environmental Problems
Edited by
Sanat K. Dhar
De Paul University
Chicago, Illinois
PLENUM PRESS • NEW YORK. LONDON
Library of Congress Cataloging in Publication Data
Main entry under title:
Metal ions in biological systems: studies of some biochemical and environmental
problems.
(Advances in experimental medicine and biology, v. 40)
Proceedings of a conference on the role of metal ions in biological systems
held Nov. 20·21, 1972, at Argonne National Laboratory, sponsored by the Central
States Universities, Inc., the Center for Educational Affairs, Argonne National
Laboratory, and the U. S. Atomic Energy Commission.
Includes bibliographies.
1. Metals in the body-Congresses. 2. Metal ions-Congresses. 3. Pollution
Congresses. I. Dhar, San at K., ed. II. Central States Universities, inc. III. United
States. Argonne National Laboratory, Lemont, Ill. Center for Educational Affairs.
IV. United States. Atomic Energy Commission. [DNLM: 1. Biochemistry-Con.
gresses. 2. Metals-Congresses. WI AD559 v. 40 1972 I QV290 M584 1972]
QP531.M47 612' .0152 73·15981
ISBN-13: 978-1-4684-3242-8 e-ISBN-13: 978-1-4684-3240-4
DOl: 10.1007/978-1-4684-3240-4
Conference Committee: S. S. Danyluk, S. K. Dhar (Chairman), L. W. Dini,
A. B. Edmundson, L. I. Katzin, A. Lindenbaum, J. J. Nisbet, D. O. Van Ostenburg,
J. Shen·Miller, M. Schiffer.
Sponsors: Central States Universities, Inc., 9700 South Cass Avenue, Argonne,
Illinois; Argonne Center for Educational Affairs, Argonne National Laboratory,
Argonne, Illinois; U.S. Atomic Energy Commission.
Proceedings of a conference on the Role of Metal Ions in
Biological Systems held November 20 and 21, 1972, at
Argonne National Laboratory.
© 1973 Plenum Press, New York
A Division of Plenum Publishing Corporation
227 West 17th Street, New York, N.Y.I0011
Softcover reprint of the hardcover I 5t edition 1973
United Kingdom edition published by Plenum Press, London
A Division of Plenum Publishing Company, Ltd.
Davis House (4th Floor), 8, Scrubs Lane, Harlesden, London, NW 10 6 SE, England
All rights reserved
No part of this publication may be reproduced in any form without
written permission from the publisher
PREFACE
The articles published in this volume are based on the
papers del ivered at a conference on the Role of Metal Ions in
Biological Systems held November 20 and 21, 1972, at Argonne
National Laboratory. The purpose of the conference was to
present to an interdiscipl inary audience of physical scientists
some recent developments illustrating the chemical and environ
mental participation of the heavy metal ions in the biological
system. The invited speakers at the conference are special ists
in the fields they describe, and the articles presented here
are at a level of interest to readers with backgrounds in
physical sciences who are not necessari Iy doing research in the
areas described. The articles are referenced through 1972, and
in some cases early 1973, and thus should also be of value to
research workers. It is hoped that the book wi II be of particular
interest to chemists, biologists, workers in the fields of en
vironmental science and publ ic health, as wei I as graduate and
senior undergraduate students in these discipl ines.
The conference was sponsored by the Central States
Universities, Inc., a consortium of sixteen midwestern univer
sities, the Center for Educational Affairs, Argonne National
Laboratory, and the United States Atomic Energy Commission. It
is my pleasure to thank the members of the conference committee
for their ideas and active help in organizing the conference.
I am also indebted to many of my col leagues at various CSUI in
stitutions for their valuable suggestions towards the implemen
tation of the conference. Special thanks are due to Mrs. Dorothy
Carlson and her associates at Argonne National Laboratory for
the i r flaw Ie ss arrangements. I wou I d a I so like to thank Mrs.
Susan O'Brien for her ski I Iful typing of the photocopy of the
vi i
vii i PREFACE
manuscript. Finally, I wish to thank my fami Iy for their wi I ling
sacrifice during the period the conference and assembl ing this
volume took much of my time that was their due.
Sanat K. Dhar
LIST OF CONTRIBUTORS
N. A. Berger, Laboratory of Molecular Aging, National Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
B. G. Blaylock, Environmental Sciences Division, Oak Ridge National
Laboratory, Oak Ridge, Tennessee
J. J. Butzow, Laboratory of Molecular Aging, National Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
P. Clark, Laboratory of Molecular Aging, National Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
G. L. Eichhorn, Laboratory of Molecular Aging, National Institutes
of Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
Karl D. Hardman, Division of Biological and Medical Research, Argonne
National Laboratory. Argonne, I I I inois
Rolf Hartung, Department of Environmental and Industrial Health, The
University of Michigan, Ann Arbor, Michigan
Paul R. Harrison, Department of Environmental Control, City of
Chicago, Chicago, I I I inois
J. Heim, Laboratory of Molecular Aging, National Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
ix
x LIST OF CONTRIBUTORS
Arthur Lindenbaum, Division of Biological and Medical Research,
Argonne National Laboratory, Argonne, I I I inois
A. S. Mi Idvan, The Institute for Cancer Research, Fox Chase, Phi la
delphia, Pennsylvania
J. B. Nei lands, Department of Biochemistry, University of California,
Berkeley, California
J. Pitha, Laboratory of Molecular Aging, National Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
G. H. Reed, Department of Biophysics and Physical Biochemistry,
Johnson Research Foundation, University of Pennsylvania School
of Medicine, Phi ladelphia, Pennsylvania
C. Richardson, Laboratory of Molecular Aging, National Institutes
of Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore,
r~aryland
J. M. Rifkind, Laboratory of Molecular Aging, National Institutes
of Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
Jack Schubert, Department of Radiation Health, Graduate School of
Publ ic Health, University of Pittsburgh, Pittsburgh, Pennsylvania
Michael C. Scrutton, Department of Biochemistry, Temple University
School of Medicine, Phi ladelphia, Pennsylvania
Y. Shin, Laboratory of Molecular Aging, National .Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
E. Tarien, Laboratory of Molecular Aging, National Institutes of
Health, Gerontology Research Center, Baltimore City Hospitals,
Baltimore, Maryland
Bert L. Val lee, Biophysics Research Laboratory, Department of Bio
logical Chemistry, Harvard Medical School, and the Division of
Medical Biology, Peter Bent Brigham Hospital, Boston,
Massachusetts
