Table Of ContentMECHANISMS IN THE
PATHOGENESIS OF
ENTERIC DISEASES
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
!RUN R. COHEN, The Weizmann Institute of Science
DAVID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, N. S. Kline Institute for Psychiatric Research
RODOLFO PAOLETTI, University of Milan
Recent Volumes in this Series
Volume 407
EICOSANOIDS AND OTHER BIOACTIVE LIPIDS IN CANCER, INFLAMMATION,
AND RADIATION INJURY 3
Edited by Kenneth V. Honn, Lawrence J. Marnell, Santosh Nigam, Robert L. Jones,
and Patrick Y-K Wong
Volume 408
TOWARD ANTI-ADHESION THERAPY FOR MICROBIAL DISEASES
Edited by Itzhak Kahane and ltzhak Ofek
Volume 409
NEW HORIZONS IN ALLERGY IMMUNOTHERAPY
Edited by Alec Sehon, Kent T. HayGlass, and Dietrich Kraft
Volume 410
FRONTIERS IN ARTERIAL CHEMORECEPTION
Edited by Patricio Zapata, Carlos Eyzaguirre, and Robert W. Torrance
Volume 411
OXYGEN TRANSPORT TO TISSUE XVIII
Edited by Edwin M. Nemoto and Joseph C. LaManna
Volume 412
MECHANISMS IN THE PATHOGENESIS OF ENTERIC DISEASES
Edited by Prem S. Paul, David H. Francis, and David A. Benfield
Volume 413
OPTICAL IMAGING OF BRAIN FUNCTION AND METABOLISM II: Physiological
Basis and Comparison to Other Functional Neuroimaging Methods
Edited by Arno Villringer and Ulrich Dirnagl
Volume 414
ENZYMOLOGY AND MOLECULAR BIOLOGY OF CARBONYL METABOLISM 6
Edited by Henry Weiner, Ronald Lindahl, David W. Crabb, and T. Geoffrey Flynn
Volume 415
FOOD PROTEINS AND LIPIDS
Edited by Srinivasan Damodaran
Volume 416
PLATELET-ACTIVATING FACTOR AND RELATED LIPID MEDIATORS 2: Roles in
Health and Disease
Edited by Santosh Nigam, Gert Kunkel, and Stephen M. Prescott
A Continuation Order Plan is available for this series. A continuation order will bring delivery of each new volume
immediately upon publication. Volumes are billed only upon actual shipment. For further information please contact
the publisher.
MECHANISMS IN THE
PATHOGENESIS OF
ENTERIC DISEASES
Edited by
Prem S. Paul
Iowa State University
Ames, Iowa
David H. Francis
South Dakota State University
Brookings, South Dakota
and
David A. Benfield
South Dakota State University
Brookings, South Dakota
SPRINGER SCIENCE+BUSINESS MEDIA, LLC
Llbrary of Congress Cataloglng-ln-Publlcatlon Data
Mechan1s•s in the pathogenesis of enteric d1seases 1 edited by Preo S.
Paul, David H. Francis, and David A. Benfield.
p. c•. -- <Advances 1n experioental oedicine and biology ; v.
412)
"Proceed1ngs of the First International Rushoore Conference on
Mechanisos in the Pathogenesis of Enteric Dlseases. held Septeober
28-30. 1995, In Rapid City, South Dakota"--T.p. verso.
Includes bibliographical references and index.
ISBN 978-1-4899-1830-7 ISBN 978-1-4899-1828-4 (eBook)
DOI 10.1007/978-1-4899-1828-4
1. Infect1on--Pathogenes1s--Congresses. 2. Cooounicable diseases-
-Pathogenes1s--Congresses. I. Paul. Preo S. II. Francis, David H.
III. Benf1eld, David A. IV. International Rushoore Conference on
Mechan1sms 1n the Pathogenesis of Enteric Diseases 11st 1995
Rap 1d C 1t y. S. D. l V. Ser ies.
[DNLM· 1. Gastrointestinal Diseases--physlopathology--congresses.
2. Gastro1ntestinal Diseases--etiology--congresses. Wl AD559 v.412
1997 1 WI 140 M486 1997]
RB153.M43 1997
616.3'307--dc21
DNLM/DLC
for Library of Congress 97-5561
CIP
Proceedings of the First International Rushmore Conference on Mechanisms in the Pathogenesis of Enteric
Diseases, held September 28-30, 1995, in Rapid City, South Dakota
ISBN 978-1-4899-1830-7
© 1997 Springer Science+Business Media New York
Originally published by Plenum Press, New York in 1997
Softcover reprint of the hardcover 1s t edition 1997
http:// www. plenum.com
10987654321
Ali rights reserved
No part ofthis book may be reproduced, stored in a retrieval system, or transmitted in any form or by any
means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written
permission from the Publisher
PREFACE
This book, Mechanisms in the Pathogenesis of Enteric Diseases, is the outcome of
the First International Rushmore Conference on Mechanisms in the Pathogenesis of En
teric Diseases, held in September 1995 at Rapid City, South Dakota. The meeting was or
ganized by members of the North-Central Regional Research Committee "NC-62," a
United States Department of Agriculture-sponsored consortium of swine enteric disease
researchers from land-grant institutions. This conference was conceived as a forum for an
interdisciplinary discussion of mechanisms of infectious diseases. It was intended that
such a discussion would stimulate cross-fostering of ideas and nurture synergistic collabo
rations among scientists working on enteric diseases of humans and animals_. In atten
dance, there were more than 140 participants from the United States and 12 foreign
countries representing all of the world's continents. Participants brought expertise from
many disciplines in both human and veterinary medicine. Multiple perspectives and an in
formal atmosphere provided an environment for lively and thought-provoking discussions.
Conference topics included Pathobiology of Gastroenteric Diseases, Mechanisms of Iden
tity and Interaction between Host and Pathogen, Effector Mechanisms in the Pathogenesis
of Enteric Diseases, Regulation of Pathogenic Activity in Enteric Diseases, and Novel Ap
proaches to Prevention and Therapy of Enteric Diseases.
Ten internationally renowned scientists gave keynote presentations in addition to 30
oral presentations and 39 poster presentations. The keynote speakers were Drs. Harley
Moon, USDA Plum Island Animal Disease Center, Greenport, New York, USA, presently
at Iowa State University; Robert Argenzio, College of Veterinary Medicine, North Caro
lina State University, Raleigh, North Carolina, USA; Kathryn Holmes, University of Colo
rado Health Sciences Center, Denver, Colorado, USA; Michele Mouricout, Faculty of the
Sciences, University of Limoges, Limoges, France; Harry Greenberg, Stanford University
School of Medicine, Stanford, California, USA; Kirsten Sandvig, Institute for Cancer Re
search, The Norwegian Radium Hospital, Oslo, Norway; James Kaper, University of
Maryland School of Medicine, Baltimore, Maryland, USA; Brett Finlay, University of
British Columbia, Vancouver, Canada (Dr. Finlay's speech was given by Murry Stein of
his laboratory); Dr. Charles Elson, Department of Medicine, University of Alabama at
Birmingham, Birmingham, Alabama, USA; and Dr. Mary Estes, Division of Molecular
Virology, Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
The reviews prepared by the keynote speakers provided latest information on the mecha
nisms of pathogenesis of enteric diseases and will serve as authoritative references for re
searchers working on enteric diseases.
Members of the conference organizing committee were Drs. David Francis and
David Benfield, South Dakota State University; Lynn Joens, University of Arizona, Rod-
v
vi Preface
ney Moxley, University of Nebraska; Mike Murtaugh, University of Minnesota; Prem
Paul and Mike Wannemuehler, Iowa State University; and Linda Saif, Ohio State Univer
sity. Drs. Francis and Benfield were the conference hosts.
The results of a survey of conference participants indicated a high degree of satis
faction with the meeting. Participants expressed enthusiastic support for a second meeting
on the same subject. Hence, the organizing committee is pleased to announce the Second
International Rushmore Conference on Mechanisms in the Pathogenesis of Enteric Dis
eases to be held in Rapid City, South Dakota, USA, September 30 through October 3,
1998. All enteric disease researchers and interested persons are invited to attend the con
ference. The conference organizing committee expresses sincere thanks to all who gave
presentations at the First International Rushmore Conference, those who joined in discus
sions, and participants who provided an enthusiastic environment. The organizing com
mittee expresses special gratitude to organizations whose financial support made the
conference possible.
Financial contributions were from the following sources: USDA NRICGP (confer
ence support grant); NSF EPSCoR of South Dakota, USA; Sayer-Agriculture Division,
Shawnee Mission, Kansas, USA; Pfizer-Central Research Division, Lincoln, Nebraska,
USA; Solvay Animal Health, Mendota Heights, Minnesota, USA; Boehringer Ingelheim
Animal Health, Saint Joseph, Missouri, USA; Grand Laboratories, Inc., Freeman, South
Dakota, USA; Ambico, Inc., Dallas Center, Iowa, USA; Eli Lilly and Company Founda
tion, Indianapolis, Indiana, USA; NOBL Laboratories, Inc., Sioux Center, Iowa, USA;
Oxford Veterinary Labs, Inc., Worthington, Minnesota; Rural Technologies, Inc., Brook
ings, South Dakota, USA; and the College of Agriculture and Biological Sciences, the Ag
ricultural Experiment Station, and the Department of Veterinary Science, South Dakota
State University, USA.
Prem S. Paul
David Francis
David A. Benfield
CONTENTS
I. Comparative Histopathology of Intestinal Infections
Harley W. Moon
2. Neuro-Immune Pathobiology of Infectious Enteric Disease 21
Robert A. Argenzio
3. Application oflntestinal Xenografts to the Study of Enteropathogenic Infectious
Disease . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Howard B. Gelberg, Joseph D. Thulin, and Mark S. Kuhlenschmidt
4. An Overview of Immunological and Genetic Methods for Detecting Swine
Coronaviruses, Transmissible Gastroenteritis Virus, and Porcine
Respiratory Coronavirus in Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Theerapol Sirinarumitr, Prem S. Paul, Patrick G. Halbur, and John P. Kluge
5. Pathogenesis of0157:H7 Escherichia coli Infection in Neonatal Calves 47
Evelyn A. Dean-Nystrom, BradT. Bosworth, and Harley W. Moon
6. Variation in Virulence in the Gnotobiotic Pig Model of0157:H7 Escherichia
coli Strains of Bovine and Human Origin . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Diane R. Baker, Rodney A. Moxley, and David H. Francis
7. Attaching and Effacing E. coli: Microscopic and Ultrastructural Observations of
Intestinal Infections in Pigs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
R. J. Higgins, G. R. Pearson, and C. Wray
8. Dynamics of Clostridium dif.ficile Infection: Control Using Diet 63
Peter B. Ward and Graeme P. Young
9. Detection and Differentiation of 3 K88 Serogroups Using Polymerase Chain
Reaction Techniques: K88 Serogroup Detection and Differentiation 77
M.A. Franklin and A. G. Mathew
10. Specific Identification of Escherichia coli 0157: H7 Using a Multiplex PCR
Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Y. P. 1. Gannon, S. D'Souza, T. Graham, and R. K. King
vii
viii Contents
II. Variation in Manifestation of E. coli H7 Antigen . . . . . . . . . . . . . . . . . . . . . . . . . . 83
C. W. Bailey and C. A. Carson
12. Verotoxigenic Escherichia coli in Slaughter Cattle and Ground Beef in South
Dakota . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
Christine J. Reitsma, David R. Henning, Christopher Chase, and
David J. Hurley
13. Immunoglobulin Response to Salmonella enteritidis Outer Membrane Proteins:
Use for Evaluating Infectious Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
Carol W. Maddox, Suzanne E. Baker, Patricia A. Dunn, and
Anthony E. Castro
14. Sequence Analysis ofVP7 Gene of a Bovine Rotavirus with G6 Subtype 93
Srinivas Mummidi, Prem Paul, and Robert Holland
15. Detection of the Fimbria! Gene F18 (F107) from Swine Enteritis Escherichia
coli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Linda Schultz, William Fales, Carol Maddox, and James Turk
16. A Chick Model for the Study of" Attaching and Effacing Escherichia coli''
Infection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
M. Sueyoshi, M. Nakazawa, and S. Tanaka
17. Immunological Cross Reactivity ofEAEA (Intimin) from E. coli that Cause
Attaching and Effacing Lesions in Humans and Rabbits . . . . . . . . . . . . . . . I 03
Tonia S. Agin and Marcia K. Wolf
18. Characterization of the EAEA Protein of Attaching and Effacing Escherichia
coli 045 from Pigs Using Monoclonal Antibodies . . . . . . . . . . . . . . . . . . . 105
Chengru Zhu and John M. Fairbrother
19. Interactions between the Enteric Pathogen and the Host: An Assortment of
Bacterial Lectins and a Set ofGlycoconjugate Receptors . . . . . . . . . . . . . . 109
Michele Mouricout
20. Virus-Receptor Interactions in the Enteric Tract: Virus-Receptor Interactions . . . 125
K. V. Holmes, D. B. Tresnan, and B. D. Zelus
21. Characterization of a Porcine Enterocyte Receptor for Group A Rota virus 135
Mark S. Kuhlenschmidt, Mark D. Rolsma, Theresa B. Kuhlenschmidt, and
Howard B. Gelberg
22. A 50 kDa Membrane Protein from Bovine Kidney Cells Is a Putative Receptor
for Bovine Viral Diarrhea Virus (BVDV) . . . . . . . . . . . . . . . . . . . . . . . . . . . 145
H. C. Minocha, W. Xue, and J. R. Reddy
23. Fimbria! Adhesins of Salmonella typhimurium: Role in Bacterial Interactions
with Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
A. J. Baumler, R. M. Tsolis, and F. Heffron
Contents ix
24. Phenotypic and Genotypic Profiles of Human, Canine, and Porcine Spirochetes
Associated with Colonic Spirochetosis Correlates with in Vivo Brush
Border Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
N. Muniappa and G. E. Duhamel
25. A Three-Receptor Model for the Interaction of the K88 Fimbria! Adhesin
Variants of Escherichia coli with Porcine Intestinal Epithelial Cells 167
Alan K. Erickson, Lloyd 0. Billey, Geetha Srinivas, Diane R. Baker, and
David H. Francis
26. Fimbria! Colonisation Factors FI8AB and FI8AC of Escherichia coli Isolated
from Pigs with Postweaning Diarrhea and Edema Disease . . . . . . . . . . . . . 175
H. Imberechts, H. U. Bertschinger, B. Nagy, P. Deprez, and P. Pohl
27. Plasminogen Receptors: Turning Salmonella and Escherichia coli into
Proteolytic Organisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
T. K. Korhonen, K. Liihteenmaki, M. Kukkonen, R. Pouttu, U. Hynonen,
K. Savolainen, B. Westerlund-Wikstrom, and R. Virkola
28. Evaluation of DNA "Fingerprinting" for Predicting the Potential of E. coli
0157:H7 Isolates to Cause Hemolytic Uremic Syndrome (HUS) . . . . . . . . 193
Kelly K. McAdoo and C. A. Carson
29. Fermentation and Growth Response of a Primary Poultry Isolate of Salmonella
typhimurium Grown under Strict Anaerobic Conditions in Continuous
Culture and Amino Acid-Limited Batch Culture . . . . . . . . . . . . . . . . . . . . . 20 I
K. G. Maciorowski, D. J. Nisbet, S.D. Ha, D. E. Corrier, J. R. DeLoach, and
S.C. Ricke
30. Distribution ofK88-Adhesive and Non-Adhesive Phenotypes among Four
Popular Breeds of Pigs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209
Diane R. Baker, Lloyd 0. Billey, and David H. Francis
3 I. Elucidating the Cell Entry Mechanisms of Porcine Rota viruses . . . . . . . . . . . . . . 213
Rafael A. Garduno, Andrew Brevik, and David A. Benfield
32. Adherence and Invasion of Aeromonas caviae to Monolayer Cells: Adherence
and Invasion of Aeromonas caviae . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217
Jonathan Shaw, Jonathan Thomley, and Adrian Eley
33. FI07-Binding Immunoassay Detects Porcine Intestinal Receptors for FI07
Fimbriae of Escherichia coli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221
Margrit Stamm, Evelyn A. Dean-Nystrom, and BradT. Bosworth
34. Intracellular Transport and Processing of Protein Toxins Produced by Enteric
Bacteria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225
K. Sandvig, 0. Garred, and B. van Deurs
35. Murine Model ofRotavirus Infection................................... 233
Ningguo Feng, Manuel A. Franco, and Harry B. Greenberg
