Mass Spectrometry–Based Glycoproteomics and Its Clinic Application

259 Pages·2021·8.381 MB·English

by  Haojie Lu

Mass Spectrometry–Based
Glycoproteomics and
Its Clinic Application

Mass Spectrometry–Based
Glycoproteomics and
Its Clinic Application
Edited by
Haojie Lu
First edition published 2022
by CRC Press
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Library of Congress Cataloging‑in‑Publication Data
Names: Lu, Haojie, 1974- editor.
Title: Mass spectrometry based glycoproteomics and its clinic application / edited
by Haojie Lu.
Description: First edition. | Boca Raton, FL : CRC Press, 2021. | Includes bibliographical
references and index. | Summary: “This book demonstrates the progress that has been
achieved in Mass spectrometry based glycoproteomic analysis, including a wide range
of analytical methodologies and strategies involved in selective enrichment, qualitative
analysis, quantitative analysis and data analy.sis, together with their clinical applications.
The book will serve as a valuable resource to aid appropriate techniques and their
applications for students, postdocs, and researchers working in proteomics, glycoscience,
analytical chemistry, biochemistry and clinical medicine”-- Provided by publisher.
Identifiers: LCCN 2021001208 (print) | LCCN 2021001209 (ebook) | ISBN
9781032028613 (hardcover) | ISBN 9781003185833 (ebook)
Subjects: LCSH: Glycoproteins--Analysis. | Mass spectrometry--Methodology.
Classification: LCC QP552.G59 M35 2021 (print) | LCC QP552.G59 (ebook) |
DDC 572/.68--dc23
LC record available at https://lccn.loc.gov/2021001208
LC ebook record available at https://lccn.loc.gov/2021001209
ISBN: 978-1-032-02861-3 (hbk)
ISBN: 978-1-032-02923-8 (pbk)
ISBN: 978-1-003-18583-3 (ebk)
DOI: 10.1201/9781003185833
Typeset in Minion
by KnowledgeWorks Global Ltd.
Contents
Editor, vii
Contributors, ix
Chapter 1 ◾ S elective Enrichment Methods for N-Glycosite
Containing Peptides in N-Glycoproteomics 1
Guoli WanG, YinG ZhanG, and haojie lu
Chapter 2 ◾ Q uantitative Methods for N-Glycosite
Containing Peptides in N-Glycoproteomics 29
ZhenYu Sun, YinG ZhanG, and haojie lu
Chapter 3 ◾ Q ualitative and Quantitative Methods
for N-Glycans in N-Glycomics 55
ShifanG ren and haojie lu
Chapter 4 ◾ Q ualitative and Quantitative Analytical
Methods for Intact Glycopeptides 91
Weiqian Cao and penGYuan YanG
Chapter 5 ◾ A nalytical Software and Databases
in N-Glycoproteomics 131
SuidenG qin and Zhixin tian
Chapter 6 ◾ C linical Applications 167
CaiYun fanG and haojie lu
INDEX, 237
v

Editor
Haojie Lu, PhD, is a Professor at Fudan University since 2003. He earned
his PhD from Lanzhou Institute of Chemical Physics, Chinese Academy
of Sciences in 2001. He specializes in proteomics based on mass spectrom-
etry with particular emphasis on novel technologies for separation and
identification of low-abundant proteins and post-translationally modified
proteins (including glycosylation), as well as relative and absolute quan-
tification methods for proteomics. Dr. Lu’s awards include 2009 Winner
of Young Chemist Award from China Chemical Society; 2009 University
Distinguished Professor of Shanghai (Eastern Scholar); 2010 supported by
The National Science Fund for Distinguished Young Scholars; and 2013
he was awarded the Eastern Scholar tracking program. He has authored
articles published in national and international journals.
vii

Contributors
Weiqian Cao Suideng Qin
The Fifth People’s Hospital, Fudan School of Chemical Science
University, and The Shanghai & Engineering
Key Laboratory of Medical Shanghai Key Laboratory of
Epigenetics Chemical Assessment and
The International Co-laboratory Sustainability
of Medical Epigenetics and Tongji University
Metabolism Shanghai, China
Ministry of Science and Technology
Institutes of Biomedical Sciences Shifang Ren
NHC Key Laboratory of NHC Key Laboratory of
Glycoconjugates Research Glycoconjugates Research
Fudan University Department of Biochemistry
Shanghai, China and Molecular Biology
School of Basic Medical Sciences
Caiyun Fang Fudan University
Department of Chemistry Shanghai, China
Fudan University
Shanghai, China Zhenyu Sun
Institutes of Biomedical Sciences
Haojie Lu Fudan University
Department of Chemistry Shanghai, China
Institutes of Biomedical Sciences
NHC Key Laboratory of
Glycoconjugates Research, and
Shanghai Cancer Center
Fudan University
Shanghai, China
ix