l. swathi 2013

158 Pages·2013·3.5 MB·English

GENETIC DIVERSITY ANALYSIS USING
MORPHO-PHYSIOLOGICAL AND
MOLECULAR MARKERS FOR BREEDING
YIELD AND DROUGHT TOLERANCE IN
MUNGBEAN (Vigna radiata (L.) Wilczek.)

L. SWATHI

B.Sc. (Ag.)

MASTER OF SCIENCE IN AGRICULTURE

(GENETICS AND PLANT BREEDING)

2013
GENETIC DIVERSITY ANALYSIS USING
MORPHO-PHYSIOLOGICAL AND
MOLECULAR MARKERS FOR BREEDING
YIELD AND DROUGHT TOLERANCE IN
MUNGBEAN (Vigna radiata (L.) Wilczek.)

BY

L. SWATHI

B.Sc. (Ag.)

THESIS SUBMITTED TO THE
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE AWARD OF THE DEGREE OF

MASTER OF SCIENCE IN AGRICULTURE
(GENETICS AND PLANT BREEDING)

CHAIRPERSON: Dr. D. MOHAN REDDY

DEPARTMENT OF GENETICS AND PLANT BREEDING
SRI VENKATESWARA AGRICULTURAL COLLEGE, TIRUPATI
ACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY
RAJENDRANAGAR, HYDERABAD – 500 030

2013
DECLARATION

I, L. SWATHI, hereby declare that the thesis entitled “GENETIC
DIVERSITY ANALYSIS USING MORPHO-PHYSIOLOGICAL AND
MOLECULAR MARKERS FOR BREEDING YIELD AND DROUGHT
TOLERANCE IN MUNGBEAN (Vigna radiata (L.) Wilczek)” submitted
to the Acharya N.G. Ranga Agricultural University for the award of
degree of Master of Science in Agriculture is the result of original research
work done by me. I also declare that no material contained in the thesis has
been published earlier in any manner.

Place : (L. SWATHI)
I.D. No.: TAM/2011-18
Date :

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Certificate

L. SWATHI has satisfactorily prosecuted the course of research and
that thesis entitled “GENETIC DIVERSITY ANALYSIS USING
MORPHO-PHYSIOLOGICAL AND MOLECULAR MARKERS FOR
BREEDING YIELD AND DROUGHT TOLERANCE IN MUNGBEAN
(Vigna radiata (L.) Wilczek)” submitted is the result of original research
work and is of sufficiently high standard to warrant its presentation to the
examination. I also certify that neither the thesis nor its part thereof has been
previously submitted by her for a degree of any university.

Date : (Dr. D. MOHAN REDDY)
Chairperson
Associate Professor
Dept. of Genetics and Plant Breeding
S.V. Agricultural College
Tirupati – 517 502, A.P.
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CERTIFICATE
This is to certify that the thesis entitled “GENETIC DIVERSITY
ANALYSIS USING MORPHO-PHYSIOLOGICAL AND MOLECULAR
MARKERS FOR BREEDING YIELD AND DROUGHT TOLERANCE
IN MUNGBEAN (Vigna radiata (L.) Wilczek)” submitted in partial
fulfilment of the requirements for the award of degree of MASTER OF
SCIENCE IN AGRICULTURE to the Acharya N.G. Ranga Agricultural
University, Hyderabad, is a record of the bonafide research work carried out
by L. SWATHI under our guidance and supervision.
No part of the thesis has been submitted by the student for any other
degree or diploma. The published part and all assistance and help received
during the course of the investigations have been duly acknowledged by the
author of the thesis.

Thesis approved by the Student’s Advisory Committee:

Chairperson : Dr. D. MOHAN REDDY ______________
Associate Professor
Dept. of Genetics and Plant Breeding
S.V. Agricultural College
TIRUPATI – 517 502, A.P.

Member : Dr. K. HARIPRASAD REDDY ______________
Professor and Head
Dept. of Genetics and Plant Breeding
S.V. Agricultural College
Tirupati – 517 502, A.P.

Member : Dr. V. RAJARAJESWARI ______________
Professor and Head
Dept. of Plant Physiology
S.V. Agricultural College
Tirupati – 517 502, A.P.

Date of final viva-voce:
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ACKNOWLEDGEMENTS
It is the lavish and boundless blessings of the almighty Lord Venkateshwara
that I have been able to complete my studies successfully hitherto and present
this humble piece of work, for which I am eternally indebted.
I am pleased to place my profound etiquette to Dr. D. Mohan Reddy,
Associate Professor, Department of Genetics and Plant Breeding, S.V. Agricultural
College, Tirupati and esteemed chairman of my Advisory committee for his
learned counsel, unstinted attention, arduous and meticulous guidance on the
work in all stages. His keen interest, patient hearing and constructive criticism
have installed in me the spirit of confidence to successfully complete the task.
I humbly place on record my respect and gratitude to Dr. K. Hariprasad
Reddy, Professor and Head, Department of Genetics and Plant Breeding,
S.V. Agricultural College, Tirupati for his amenable and stupendous guidance
and encouragement and valuable suggestions during the period of my study.
I sincerely accentuate my everlasting gratitude to the revered member of
my Advisory Committee Dr. V. Rajarajeshwari, Professor and Head, Department
of Plant Physiology, S.V. Agricultural College, Tirupati for her valuable
guidance and cooperation throughout my period of thesis.
It’s an immense pleasure to express great, heartfelt respect to
Dr. M. Reddy Sekhar, Professor, Dr. M. Shanthi Priya, Assistant Professor
Department of Genetics and Plant Breeding, S.V. Agricultural College,
Tirupati for their help and guidance during the period of my study which
enabled the successful completion of my work.
Its gives me great pleasure to express my deep sense of gratitude to
Dr. P. Sudhakar, Senior Scientist, Department of Plant Physiology, Dr. P. Latha,
Scientist, Department of Plant Physiology and Dr. B.V. Bhaskara Reddy, Senior
Scientist, Department of Plant Pathology, Institute of Frontier Technology, RARS,
Tirupati for their help and guidance for successful completion of my investigation.
With profound respect, I extend my gratitude to Dr. N.V. Naidu,
Associate Dean for extending necessary facilities for the investigation.
I express my heartfelt thanks to Mr. Lokanadam, Farm Manager and
Mr. Mahendra, A.E.O for their timely co-operation in the farming operations.
My deepest and propound gratitude would aptly sum up my feelings
towards my beloved teachers Dr. M. Lal Ahamed, Dr. P. Anil Kumar,
Dr. Raghunadh Reddy and Dr. Krishna Prasad Ji who have guided me all the
way to reach up to this level.
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I allocate my highest respect and whole hearted regards from my inner
core of my heart to my ever loved Parents, Smt. Lakshmi and Sri. L. Avathara
Murthy, who have given me life and taught the concepts of life and their
dedicated efforts to educate me to this level and without whose valuable moral
support, the thesis would not have seen the light of the day. They are ineffable
and to their benevolent feet of, I am dedicating this thesis with great pleasure.
With boundless affection, I owe an encompassing debt to my beloved
brother L. Vasanth Naidu for his constant encouragement throughout my
career. He has been fountain of inspiration throughout my life without whose
help in every walk of life this work would not have been possible.
No words are enough to express the affection to my special friends,
Kapil, Gowtham, Yasar, Susmita, Vineela, Surekha, Sunitha and Anitha for
their help, constant encouragement and companionship in my personal and
professional life for moral support during research work.
I express my heartfelt gratitude and thanks to my seniors, Rama,
Nagraj, Govardan, Saida Naik, Narasimhulu, Deepa, A.B.M Sireesha and
Haritha for their valuable guidance and encouragement.
It is a pleasure to acknowledge the affection and inspiration rendered by
my classmates, Chandana, Revi, Praveen, Santosh, Rajesh, Sunil, Srihari,
Nazeer, Siva, Manasa and my juniors Sai Rekha, Shreya, Kiranmay, Shilpa,
Mallikarjuna, Paramesh, Imtiaz and Bhaskar.
Heartful thanks are due to my friends, Sruthi, Sujatha, Ramya,
Kundana, Purushotam, Sharif and Rajesh for their timely help during my
investigation.
I acknowledge the help of Munikrishna and Naidu, non-teaching staff
of Department of Genetics and Plant Breeding, S.V. Agricultural College,
Tirupati.
I humbly thank the authorities of Acharya N.G. Ranga Agricultural
University, Hyderabad for providing me the opportunity in getting admission
into Post graduation and also for all the facilities extended during the period of
my post-graduation programme.
Finally I express my special thanks to Venkat for his timely help in
printing and binding work of my thesis.
Brain may forgotten to mention, but heart will remember and
Everyone is not mentioned, but no one is forgotten.

Swathi…
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CONTENTS
Chapter No. Title Page No.
I INTRODUCTION 1 – 4
II REVIEW OF LITERATURE 5 – 33
III MATERIAL AND METHODS 34 – 62
IV RESULTS AND DISCUSSION 63 – 123
V SUMMARY AND CONCLUSIONS 124 – 127
LITERATURE CITED 128 – 136

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LIST OF TABLES

Table Page
Title
No. No.
3.1 Salient features of mungbean genotypes 35-36
3.2 List of primers 61
4.1 Analysis of variance for fifteen quantitative characters in 64
mungbean
4.2 Mean performance of thirty one genotypes of mungbean 66
for fifteen quantitative characters
4.3 Mean coefficient of variability, heritability (broad sense), 73
genetic advance as per cent of mean for fifteen characters
in thirty one mungbean genotypes
4.4 Analysis of variance for dispersion in thirty one 78
genotypes of mungbean
4.5 Cluster composition of thirty one mungbean genotypes 79
(Tocher’s method)
4.6 Intra cluster (diagonal) and inter-cluster distances for 82
seven clusters in mungbean
4.7 Mean performance of the clusters with respect to 83
different characters
4.8 Contribution of different quantitative characters to 86
diversity in mungbean
4.9 Phenotypic (r ) and genotypic (r ) correlation coefficients 91
p g
among fifteen characters in thirty one genotypes of
mungbean
4.10 Phenotypic (p) and genotypic (g) path coefficients 100
among grain yield per plant and other yield components
in mungbean
4.11 Analysis of variance for various treatments of PEG in 31 107
mungbean genotypes under laboratory
4.12 Mean performance of 31 mungbean genotypes at various 109
concentrations of PEG
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Table Page
Title
No. No.
4.13 Non-parametric selection of mungbean genotypes based 115
on PEG screening and yield
4.14 Characteristics of amplification products obtained from 117
twelve RAPD primers
4.15 Average similarity coefficients of 12 mungbean 121
genotypes

LIST OF ILLUSTRATIONS

Figure Page
Title
No. No.
4.1 Dendrogram of thirty one mungbean genotypes obtained 80
through Tocher’s method of classification
4.2 Intra-cluster (D) and inter-cluster distance (D2) among 84
seven clusters of mungbean
4.3 Dendrogram showing various genetic relationship among 122
12 mungbean genotypes

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