Table Of ContentKININS IV
Part A
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY
Editorial Board:
NATHAN BACK, State University of New York at Buffalo
NICHOLAS R. DI LUZIO, Tulane University School of Medicine
EPHRAIM KATCHALSKI-KATZIR, The Weizmann Institute of Science
DAVID KRITCHEVSKY, Wistar Institute
ABEL LAJTHA, Rockland Research Institute
RODOLFO PAOLETTI, University of Milan
Recent Volumes in this Series
Volume 192
MECHANISMS OF STIMULUS-RESPONSE COUPLING IN PLATELETS
Edited by J. Westwick, M. F. Scully, D. E. MacIntyre, and V. V. Kakkar
Volume 193
METHODS IN PORPHYRIN PHOTOSENSITIZATION
Edited by David Kessel
Volume 194
MYOCARDIAL AND SKELETAL MUSCLE BIOENERGETICS
Edited by Nachman Brautbar
Volume 195A
PURINE AND PYRIMIDINE METABOLISM IN MAN V, Part A: Clinical Aspects
Including Molecular Genetics
Edited by W. L. Nyhan, L. F. Thompson, and R. W. E. Watts
Volume 195B
PURINE AND PYRIMIDINE METABOLISM IN MAN V, Part B: Basic Science Aspects
Edited by W. L. Nyhan, L. F. Thompson, and R. W. E. Watts
Volume 196
STEROID HORMONE RESISTANCE: Mechanisms and Clinical Aspects
Edited by George P. Chrousos, D. Lynn Loriaux, and Mortimer B. Lipsett
Volume 197
BIOLOGICAL REACTIVE INTERMEDIATES III: Mechanisms of Action in
Animal Models and Human Disease
Edited by James J. Kocsis, David J. Jollow, Charlotte M. Witmer,
Judd O. Nelson, and Robert Snyder
Volume 198A
KIN INS IV, Part A
Edited by Lowell M. Greenbaum and Harry S. Margolius
Volume 198B
KININS IV, Part B
Edited by Lowell M. Greenbaum and Harry S. Margolius
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KININS IV
Part A
Editedhy
Lowell M. Greenbaum
Medical College of Georgia
Augusta, Georgia
and
Harry S. Margolius
Medical University of South Carolina
Charleston, South Carolina
PLENUM PRESS • NEW YORK AND LONDON
Library of Congress Cataloging in Publication Data
International Kinin Congress (4th: 1984: Savannah, Ga.)
Kinins IV.
(Advances in experimental medicine and biology; v. 198)
"Proceedings of the Fourth International Kinin Congress, held October 21-25, 1984, in
Savannah, Georgia" - T.p. verso.
Includes bibliographies and index.
1. Kinins-Congresses. 2. Kallikrein-Congresses. I. Greenbaum, Lowell M. II.
Margolius, Harry S. III. Title. IV. Title: Kinins 4. V. Series. [DNLM: 1. Kinins
congresses. WI AD559 v.198/QU 68 1593 1984k]
QP552.K5154 1984 599/.019/24 86-9403
ISBN-13: 978-1-4684-5145-0 e-ISBN-13: 978-1-4684-5143-6
DOl: 10.1007/978-1-4684-5143-6
Proceedings of the Fourth International Kinin Congress,
held October 21-25, 1984, in Savannah, Georgia
© 1986 Plenum Press, New York
Softcover reprint of the hardcover 1s t edition 1986
A Division of Plenum Publishing Corporation
233 Spring Street, New York, N.Y. 10013
All rights reserved
No part of this book may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming,
recording, or otherwise, without written permission from the Publisher
PREFACE
Scientists from 25 countries came together at the Hyatt Regency
Hotel on October 21-25, 1984, for the 4th International Kinin Congress
in the beautiful city of Savannah, Georgia. Many of the delegates
enjoyed southern hospitality for the first time. The friendly city with
its streets lined with the Live Oak Tree (symbol of the Congress), the
balmy weather, and the excellent facilities of the hotel set the stage
for scientific events and exchange that proved so successful. The organ
ization of the meeting was the result of many hours, days and weeks of
effort by many, including from Augusta Drs. James H. Sutherland, John
Catravas, William Davis, Jr., and Hiroshi Okamoto; and from Charleston,
SC, Julie Chao, Ronald Mayfield and Donald Miller. Special thanks go
to Ms. Cher Cornett of the Department of Medical Illustration at the
Medical College of Georgia for her talent in the design of the logo, as
well as the graphics of the program and abstracts. We are indeed
indebted to Dr. Paul Brucker, Director of the Division of Health Communi
cations at the Medical College of Georgia, for his considerable aid in
spending time with us to arrange the programming format and continuity.
Ms. Sandra Usry provided very excellent and devoted secretarial help for
several years while the Congress was in the making. Thanks also to the
secretarial staff of the Department of Pharmacology at MCG, including
Rosiland Simmons and Jennie Doby for their expert assistance during the
meeting. We also wish to thank the many sponsors (NIH, Johnson &
Johnson, Bayer AG and Bayer AG/Miles, KabiVitrum AB, American Heart
Association, Medical College of Georgia Research Institute, Rich's
Travel, Bayer Yakhuin, Ltd., Bristol-Myers Co., Drug Science Foundation
of S.C., Sanwa Kagaku Ltd., Upjohn Co., Hoffmann-La Roche, Inc.,
Boehringer Ingelheim, Ltd., Burroughs Wellcome Co., Ciba Geigy Pharma
ceutical, E. R. Squibb & Sons, Inc., Fujimoto Pharmaceutical Co., Ltd.,
Am. Cyanamid Co., G. D. Searle, E. I. DuPont de Nemours & Co.).
During the early planning of the Congress, we learned of the death
of one of the world's great pharmacologists and discoverer of brady
kinin, Professor Mauricio Rocha e Silva of Brazil. Professor Rocha e
Silva not only coined the name for bradykinin but was a staunch advocate
in his own special way for research allover the world to elucidate the
importance of kin ins and kallikreins in health and in disease. It is
more than fitting that the 4th International Congress of Kinins was
dedicated to his memory and efforts. We were most fortunate that his
collaborator in the discovery of bradykinin and a great scientist in his
own right, Dr. Wilson Beraldo of Belo Horizonte, attended the Congress.
All of us were moved by his sincere tribute, as well as the tribute of
Dr. Mauricio Rocha e Silva, Jr., to Professor Rocha e Silva's contribu
tions to science and society. Since the close of the Congress we
learned of the deaths of three pioneers in kinin research, Dr. John
Pisano (suddenly) of the NIH, Dr. Ulla Hamburg of Finland and Dr. Marion
Webster, formerly of the NIH. Each of these have left lasting impres-
v
sions on us because of their pioneering contributions, their strength
of purpose in kinin research and their love of aiding others in our
discipline. We will miss them. We are privileged that these volumes
contain the last scientific communications of Drs. Pisano and Hamburg.
"Kinin '84 Savannah" had a number of "firsts". It was the first
Kinin Congress to encompass four full days. It was the first Congress
to institute "Frontier Lecturers" which provided us with information
about areas of research which may be vital to our growth but with which
we may not be familiar at this time. We are all indebted to the superb
lecturers which included Dr. Hans Fritz (FRG), Dr. Robert Colman (USA),
Dr. S. Nakanishi (Japan), Dr. John Shine (Australia), Dr. Allen Cuthbert
(England) and Dr. T. Hokfelt (Sweden). This was the first Congress to
utilize the concept of formal "Poster Discussions" so that all investi
gators had a chance to comment on their efforts in addition to the 200
posters on display. We are indebted to the discussion leaders who made
the 14 sessions so meaningful. It was the first of the Kinin Congresses
to have "competing" oral sessions and we wish to thank the Chairman and
Co-chairman of these sessions, as well as the delegates themselves, for
the excellence of the 80 oral presentations. This was the first inter
national meeting at which the T-kinin - T-kininogen sysem was intro
duced, as well as several components of the kallikrein-kinin system in
brain including kinins and kininases. The long sought-after antagonist
to bradykinin as well as the isolation and characterization of the genes
expressing kininogen synthesis was described. It was the first of our
meetings to pay tribute to our pioneers in kinin research and we again
salute the recipients, Drs. Melville Schachter (Canada), Ervin Erdos
(USA), Wilson Beraldo (Brazil), Hector Croxatto (Chile), Tomoji Suzuki
(Japan), and Gert Haberland (FRG). It was the first meeting at which
six young investigators from five countries were nominated as KabiVitrum
Scholars and given special travel awards.
Our social times together also left some unforgettable memories.
All of us will remember the warm friendship and the beauty of the
evening and as we glided down the Savannah River on the riverboat to
shuck oysters under the torchlight and accompanied by the southern jazz
music of Fort Jackson. We were truly a "Kinin Family." The satellite
meeting in Hilton Head and the visitations to Charleston and Augusta
provided additional opportunities for interactions and friendships.
The 4th International Kinin Congress was a stimulating experience
for us and we hope the pages of these volumes will keep the spirit and
science of Kinin '84 Savannah alive for many years to come.
Lowell H. Greenbaum, Ph.D
President, IKC
Augusta, Georgia
Harry S. Margolius, Ph.D, H.D.
Vice President, IKC
Charleston, South Carolina
vi
CONTENTS - PART A
TISSUE KININOGENASES
The Varied Localization and Functional Significance of
Kallikrein-Like Enzymes in Salivary Glands, Pancreas,
Colon, Sex Glands and Spermatozoa, Including
Evidence for the Presence of Nerve Growth
Factor (NGF) in Bull Sperm Acrosome.......................... I
M. Schachter, M.W. Peret, The Late C. Moriwaki,
G.D. Wheeler, R.W. Matthews, J.G. Mehta
and T. Labedz
Kininogenase from Rat Vascular Tissue.............................. 11
H. Nolly, A.G. Scicli, G. Scicli, M.C. Lama,
A.M. Guercio and O.A. Carretero
Intracellular and Intercellular Distributions of Acid
Kininogenases in Spleen...................................... 19
M. Watanabe and K. Yamafuji
Localization of Kallikreins in the Human Parotid and
Gland in the Human Kidney: A Comparative
Study of Immunohistochemistry and Enzyme
Histochemistry............................................... 27
K. Kimura and H. Moriya
Studies on Carbohydrate Structure and Immunological
Properties of Human Urinary Kallikreins...................... 35
H. Moriya, M. Ikekita and K. Kizuki
Initial Observations of a Kallikrein-Like Enzyme
Associated with the Plasma Membranes of
Rat Adipocytes............................................... 41
F. Mulholland, A. Ashford and G.S. Bailey
Bile Acids and the Intestinal Kallikrein-Kinin
System....................................................... 47
I.J. Zeitlin, H.A.R. AI-Dhahir, S. Cook,
A. Currie and K. Donovan
KININOGENS
T-Kinin and T-Kininogen - An Historical Overview................... 55
L.M. Greenbaum
Increased Plasma Level of T-Kininogen in Rats
Treated with Freund s Adjuvant............................... 61
I
A. Barlas, H. Okamoto and L.M. Greenbaum
Isolation and Properties of Two Rat Plasma
T-Kininogens. . • • • . . . . • • • • • • • • • • • • • . • . • • • • • . • . • • • • • • • • • • • • • • • • 69
H. Okamoto and L.M. Greenbaum
Characterization of Kininogen Deficiency of Brown
Norway Rat Mutant Katholiek Strain........................... 77
S. Oh-ishi, I. Hayashi, T. Ino, H. Kato,
S. Iwanaga and T. Nakano
Amino Acid Sequence of the Light Chain of Human Low
Molecular Mass Kininogen..................................... 91
F. Lottspeich, J. Kellermann, A. Henschen,
G. Rauth and W. Muller-Esterl
Limited Proteolysis of HMW Kininogen by Plasma
Kallikrein in Man- Evidence for a Processing
Mechanism Different from the Bovine System................... 97
W. Muller-Esterl, H. Hock, G. Rauth,
J. Kellermann, F. Lottspeich, and A. Henschen
Interaction of Human Low Molecular Weight Kininogen
with Human Mast Cell Tryptase................................ 105
L.B. Schwartz, M. Maier and J. Spragg
Independent Consumption of High and Low Holecular Weight
Kininogens In Vivo........................................... 113
Y. Uchida and M. Katori
Urinary Kininogen: A Possible Regulator of Kinin Formation
in Normal Individuals and Subjects with Essential
Hypertension, End-Stage Renal and Liver Disease.............. 119
M.S. Weinberg, U.M. Trebbin, and R.J. Solomon
Monoclonal Antibodies to Rat Plasma Kininogen...................... 127
G.S. Bedi and N. Back
Hydrolysis of Rat High Molecular Weight Kininogen by
Purified Rat Urinary Kallikrein: Identification
of Bradykinin as the Kinin Formed............................ 137
J.P. Girolami, F. Alhenc-Gelas, M.L. Dos Reis,
J.L. Bascands, J.M. Suc, P. Corvol. and J. Menard
Reduced or Unchanged Cofactor Function of Human High
Molecular Weight Kininogen Induced by Human
Plasma Kallikrein............................................ 147
H.T. Johansen and K. Briseid
Studies on the Antigenic Determinants of Human Low
Molecular Weight Kininogen................................... 155
T. Karkkainen, A.-C. Syvanen and U. Hamberg
Kininogen by the SRI Method in Human Serum During
an Acute Phase Inflammatory Reaction......................... 161
U. Hamberg, T. Karkkainen and T. Tallberg
Kininogen as a Pregnancy-Associated Plasma Protein................. 167
T. Karkkainen and U. Hamberg
viii
Hemodynamics of the Isolated Perfused Rat Kidney
in the Absence and Presence of Kallikrein
Substrate.................................................... 173
M. Maier, Z. Zhegu and B.R. Binder
TISSUE KALLIKREINS, KININS, AND KININASES
Active Kallikrein, Preproka11ikrein, and
Kallikrein-Inhibitor Complex................................. 181
J. Chao, L. Chao, C.M. Woodley, W. Gerald
and H.S. Margo1ius
Characterization of Rat Kallikrein-Like Mu1tigene
Family and Its Expression in the
Submandibular Gland......................................... 189
L. Chao, W. Gerald and J. Chao
Processing of Apo1ipoprotein B-l00 of Human Plasma
Low Density Lipoproteins by Tissue and
Plasma Ka11ikreins........................................... 195
A.D. Cardin, R.L. Jackson, V.H. Donaldson, J. Chao
and H.S. Margo1ius
Transporting Epithelia as Targets for Kinin Effects................ 203
A.W. Cuthbert and L.J. MacVinish
Purification and Partial Characterization of Cat
Colon and Submandibular Gland Ka11ikreins.................... 211
H. Fujimori, P.R. Levison and M. Schachter
Purification and Partial Characterization of Cat
Pancreatic and Urinary Ka11ikreins - Comparison
With Other Cat Tissue Ka11ikreins and
Related Proteases............................................ 219
H. Fujimori, P.R. Levison and M. Schachter
The Liver is the Main Organ to Clear Plasma and
Tissue Kallikreins from Rat Plasma. In Vivo.................. 229
D. Borges, C. Sampaio, P. de la Llosa and J.L. Prado
Receptor-Mediated Clearance of Tissue Ka1likreins
by Rat Liver................................................. 235
D.R. Borges, M. Kouyoumdjian, E.S. Prado
and J.L. Prado
Tissue Kal1ikreins and Related Enzymes:
Characterization by Model 01igopeptides...................... 241
E.S. Prado, L. Juliano, M.S. Araujo-Vie1
and M.A. Juliano
Release of Kallikrein and Tonin from the Rat
Submandibular Gland.......................................... 247
S.R. Maitra, S.F. Rabito and O.A. Carretero
Effect of Sodium Restriction and Corticosteroids on
Glandular Kallikrein in Plasma and in the
Submandibular Gland.......................................... 255
S.Seto, S.F. Rabito. S.R. Maitra, J.N. Wu
and O.A. Carretero
Kallikrein and Kinins Independently Stimulate Renin
Release from Isolated Rat Glomeruli.......................... 265
W.H. Beierwa1tes and O.A. Carretero
Regulation of Parotid Kallikrein Secretion-Role
of the A1pha2- and Beta-Adrenergic System................... 273
A. Rockel, A. Preiss1er, A. Heid1and
Effect of Furosemide on the Rat Submandibular
Gland Kallikrein Secretion................................... 279
O.L. Catanzaro, S.B. Vila, A. Zuccolo
and A.M. Seeber
Individual Reaction Steps in the Release of Kallidin
From Kininogen by Tissue Kallikrein.......................... 283
F. Fiedler, H. Hinz and F. Lottspeich
The Tissue Kallikrein-Kinin System in Human Seminal
Plasma - Biochemical and Functional Aspects.................. 291
E. Fink, W.-B. Schill, F. Fiedler, K. Shimamoto,
F. Krassnigg and J. Frick
New Synthetic Substrate for Kallikrein and
Its Application.............................................. 299
S. Fujii, Y. Hitomi, K. Kimura, M. Ishii,
M. Kurumi and T. Aoyama
Plasma Half-Live and Organ Uptake Ratio of
Radio1abe1ed Glandular Kallikrein in Control
and Nephrectomized Rats...................................... 309
K. Nishimura, T. Iwata and T. Kokubu
Hormonal Regulation of Glandular Kallikrein Activity
and Its Inhibitor in Human Plasma............................ 315
H. Koh, K. Uchida, S. Nambu and M. Ikeda
A Comparative Study of Proka11ikreins and
Kallikreins from Rat Pancreatic Tissue and Juice............. 323
A.A. Jaffa, M. Hussain, Z. Rashid and G.S. Bailey
An Inactive Form of Kallikrein in Human Urine...................... 329
K. Kizuki, Y. Shimamoto, M. Ikekita and H. Moriya
Purification of Inactive Kallikrein from Rat Urine................. 339
M. Takaoka, H. Okamura. T. Iwamoto and S. Morimoto
Release of Tissue Kallikrein from the Isolated
Perfused Kidney.............................................. 347
N. Lauar and K. Bhoola
Analytical Study of Kallikrein and Kallikrein-Like
Esterase Activity in Subfractions from Rat
Kidney Cortex Microsomes and Isolated
Subce 11u1ar Membranes........................................ 355
K.-J. Andersen and J. Ofstad
Endogenous Kallikrein Inhibitor in Rat Kidney
Cortex-Effect of Glucocorticoid Administration............... 361
K. Ito, K. Yamada, S. Yoshida, K. Hasunuma,
Y. Tamura and S. Yoshida
x
