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About Isolation of Lysozyme From Egg White
This document describes a method for isolating lysozyme from egg white. Key points: - Lysozyme was found to be readily adsorbed by bentonite clay. 1 gram of bentonite could adsorb the lysozyme from 80-100mL of egg white. - While lysozyme was adsorbed, it could not be eluted using aqueous buffers or other solutions. - A solution of 20% pyridine and 2% acetic acid was able to elute about 30% of the adsorbed lysozyme. Investigation found lysozyme could be efficiently eluted at the proper pH using pyridine solutions.
Detailed Information
| Author: | ['Ipung Miranti Sari'] |
|---|---|
| Publication Year: | 2011 |
| Pages: | 16 |
| Language: | English |
| Format: | |
| Price: | FREE |
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