Introduction ABS ACP ACTM AHDL ALB ALC ALP ALT AMON AMPH AMY AST BARB BENZ BUN ...

153 Pages·2001·0.44 MB·English

Introduction IGA
ABS IGG
ACP IGM
ACTM IRN
AHDL LA
ALB LDH
ALC LIP
ALP METH
ALT MG (old)
AMON MG (Revised)
AMPH OPI
AMY PALB
AST PCHE
BARB PCP
BENZ PHNO
BUN PHOS
C3 PTN
C4 RCRP
CA RIMS
CHOL SAL
CK T4
CKMB TBIL
COC THC
CRBM THEO (old)*
CREA THEO (revised)*
CRP TIBC
DABS TOBR (revised)*
DBIL TP
DGNA (non-HM) TRIG
DGTX (non-HM) TRNF
GENT TU
GGT UCFP
GLU URCA
HABS VALP
HDL VANC
IBCT
*Dependent on software revision
D-00987 December 2000 Dimension® Chemistry
Company Confidential Introduction-1
Introduction
This Chemistry Troubleshooting Guide provides information to assist you in troubleshooting all
Dimension® clinical chemistry systems.
This guide is for use by trained Dade Behring personnel only. Dade Behring Inc. does not authorize
any other use.
In order to troubleshoot method chemistry problems on a Dimension® system, you should be
familiar with the steps involved in method processing on the instrument. These are:
• Cuvette formation, one or two cuvettes per method
• Reagent or sample probe wash
• Reagent delivery to the cuvette, with or without mix
• Sample delivery to the cuvette, with mix
• Photometer read with primary and secondary optical filters
• Photometric reads captured in the filter data files
Filter data provides valuable information. You can often determine the step that caused an error by
comparing good and erroneous filter data.
The method processing steps in this guide are taken from the current method database, method insert
sheets and Dimension® service manuals.
How to Use this Guide
This guide is presented in sections, one section per method. The first item in each section is a table
describing method processing events in a condensed format. When using these tables, keep the
following in mind:
• The initial “cuvette QC” or “air blank” photometer reads are not listed as the first event for
every method because they are the same in every case.
• Reagent arm 1 (R1ARM) and reagent arm 2 (R2ARM) deliver reagent components (R1, R2,
R3 or R4) to the cuvette. The components are listed in the order they are delivered, with the
water chase (H2O) listed last.
• The PHOT READ measures the cuvette’s absorbance with primary and secondary optical
filters.
• A method may use one or two cuvettes. Activities for both cuvettes are described.
• Reagent addition with ultrasonic mix is labeled YES (Y). If there is no mix, it is labeled
NO (N).
• The sample probe adds sample with water chase (H O) volume to the cuvette, which is then
2
ultrasonically mixed.
• Type ‘a’ methods have one R1ARM delivery (single or double dip) to cuvette.
• Type ‘b’ methods have one R1ARM delivery and one R2ARM delivery to cuvette.
D-00987 December 2000 Dimension® Chemistry
Introduction-2 Company Confidential
• Type ‘c’ methods have one R1ARM delivery and multiple R2ARM deliveries or two cuvettes.
• For troubleshooting “abnormal assay” error flags, refer to the Result Monitor Troubleshooting
Guide.
• For ES, substitute R1 ARM and R2 ARM with “reagent”.
Dimension® Chemistry D-00987 December 2000
Company Confidential ABS-1
ABS (System Check for AR/ARx/RxL/XL Only)
Method Chemistry
(SABS)
Event # Event Delivery Volumes/Filters Mix
1 R1ARM (R1) 0 µL (H O) 345 µL Y
2
2 SAMPLE (C0 S04) 5 µL (H O) 50 µL Y
2 2
3 PHOT READ 510/405 nm
Total Cuvette Volume: 400 µL
First cuvette (R1BS)
Event # Event Delivery Volumes/Filters Mix
1 R1ARM (R1) 60 µL (H2O) 420 µL Y
2 PHOT READ 510/405 nm
Total Cuvette Volume: 480 µL
Second cuvette (R2BS/PQC)
Event # Event Delivery Volumes/Filters Mix
1 PHOT READ All Wavelengths
2 PHOT READ All Wavelengths
3 R2ARM (R2) 60 µL (H O) 420 µL Y
2
4 PHOT READ 510/405 nm
Total Cuvette Volume: 480 µL
Method Specifics
• Endpoint, bichromatic, 510/405 nm
• Type ‘c’ method
• Flex® type = 8 well, all liquid reagents
• R1BS and R2BS test the precision of 60-uL reagent delivery
• There is no "three cuvette" method, so a system check schedules 5 "SABS" assays (first 5
cuvettes). It then schedules the "ABS" method, which is essentially a "two cuvette" method
which alternates between "R1BS" and the "R2BS" assay. Two blank reads on the "R2BS"
cuvette prior to any fluid deliveries are used for the PQC test for all ten wavelengths. As with
the original system check, only the highest PQC result of the 5 cuvettes read will be printed on
the report slip. This results in 5 "SABS" cuvettes in a row, followed by 10 "reagent" test
cuvettes, alternating R1BS and R2BS respectively. This only holds true if there are no dirty
windows or bad cuvettes (all scheduled windows are used - none skipped)
D-00987 December 2000 Dimension® Chemistry
ABS-2 Company Confidential
• Put up: 30 tests per Flex® 5/5/5/5/5/5
cobalt sulfate
• ABS Flex® wells 1, 2, 4 & 5 contain 636 uL cobalt sulfate. Wells 3 & 6 contain 986 uL cobalt
sulfate. The additional volume is used by R3 of RMS module (RIMS).
Troubleshooting
ABS Troubleshooting
In addition to the following troubleshooting tips on “System Check,” refer to the Dimension® RxL
Operators Guide, Section 5 for basic information
“System Check” tests are chemistry free performance checks of critical fluid movement and
photometric electromechanical instrument components. Four subsystems are checked on all
instruments except for RxL’s equipped with either a HM and/or RMS Module both of which require
additional subsystem tests by the System Check routine. These tests are identified and defined as
follows:
• Photometer (a.k.a. PQC): Photometer drift QC stability test.
Specs: Wavelength drift limited to – 1.5 mAU for all wavelengths except for the 293 nm
wavelength whose limit is +/- 2.5 mAU.
• Reagent #1 (a.k.a. R1BS): Checks accuracy and precision of the sample pumps.
Specs: Assay value listed on the end flap of the ABS cartons – 12 mAU.
• Reagent #2 (a.k.a. R2BS): Checks accuracy and precision of the sample pumps.
Specs: Assay value listed on the end flap of the ABS cartons – 12 mAU.
• Sampler (a.k.a. SABS): Checks accuracy and precision of the sample pumps.
Specs: Mean = 10% of the assay value listen on the end flap of the ABS carton – 2 mAU
Keyboard System Check Map, useful for scheduling independent SC tests:
• DABS: <SHIFT + P9>
• RIMS: <SHIFT + P10>
• HABS: <CTRL + F1>
• R1BS: < CTRL + F2>
• SABS: < CTRL + F3>
• R2BS: < CTRL + F4>
• PQC: < CTRL + F5>
• W1BS: <ALT + GGT> with HM Module.
• W2BS: <ALT + GLU> with HM Module.

Troubleshoot first any Photometer System Check failures before troubleshooting any other
coincidental System Check problems. Photometric performance is primary to all other System Check
tests and must be performing normally before correcting other problems.
Note: Typically System Check performance will be better than specification by a factor of two or
better. Atypical performance near specification limits is not desirable and should not be tolerated as
it usually indicates an incipient problem.
Dimension® Chemistry D-00987 December 2000
Company Confidential ABS-3
PQC Troubleshooting
PQC problems result from the variation between two blank reads on a specific cuvette. The reads
take place approximately 43 seconds apart, and generally indicate how well the photometer can re-
position itself on that specific cuvette.
• Photometer drive belt tension too loose.
• Belt tension too high
• Photometer belt toothed ring loose on photometer casting.
• Photometer belt holding set curves from pulleys (reposition belt to fix)
• Defective, aged or incorrectly installed source lamp.
• Defective of dirty optical filter(s)
• Photometer misalignment
• Cuvette wheel misalignment
• Dirty cuvette windows
• "D" washer interference with log amp
• Log amp mounted too high
• Thermal chamber insulation interference
• Film tension-pulling cuvettes
• Cable interference and tension
• Photometer bearing needing lubrication
• Loose optics in photometer (need additional wavey washer)
• Photometer alignment off by one count (software).
D-00987 December 2000 Dimension® Chemistry
ABS-4 Company Confidential
R1BS/R2BS Troubleshooting
Accuracy
• Cuvette wheel/windows, log amp.
• Wrong coefficients
• C-Term correction required (see Service Bulletin DXL-66/ RXL-48)
• Incorrect test solution
• Wrong Bottle Value entered for ABS Lot
Precision
• Fluidics, cuvette quality, ultrasonics.
• Loose tubing or tubing fittings
• Defective syringes
• Crimped or pinched tubing
• Defective cuvettes
• Overflowing wash drains
• Worn out reagent probe tip
• Defective pump panel valve(s)
• Poor Ultrasonic mix
SABS Troubleshooting
• R1ARM delivers 345 µL of water to cuvette. Accuracy and precision problems follow same
logic as R2BS and R1BS causes.
Accuracy
• Wrong ABS sample
• Evaporated sample being reused
Precision
• Misaligned sample probe
• Worn Sample probe
• Loss of chase water or sample to sample drain
For C0 adjustment procedures, refer to the following service bulletins:
• DAR–104
• DXL–66/RxL–48
Dimension® Chemistry D-00987 December 2000
Company Confidential ACP-1
ACP
Method Chemistry
First cuvette (test)
Event # Event Delivery Volumes/Filters Mix
1 R1ARM (R1) 280 µL N
2 SAMPLE 24 µL, (H O) 40 µL Y
2
3 R2ARM (R2) 36 µL, (H O) 20 µL Y
2
4 PHOT READ 600/700 nm
Total Cuvette Volume: 400 µL
Second cuvette (blank)
Event # Event Delivery Volumes/Filters Mix
1 R1ARM (R2) 36 µL, (R1) 280 µL, (H O) 20 µL Y
2
2 SAMPLE 24 µL, (H O) 40 µL Y
2
3 PHOT READ 600/700 nm
Total Cuvette Volume: 400 µL
Method Specifics
• Endpoint, bichromatic 600/700 nm
• Type ‘c’ method
• Flex® type = 6 well
• Two cuvette method. 36 µL of R2 delivered to cuvette #1 at position 38 (very late). Every
assay causes the R2ARM to go out to position 38.
• No reagent hydration QC.
D-00987 December 2000 Dimension® Chemistry
ACP-2 Company Confidential
Troubleshooting
• Result monitor limits: High Low
A 1.25 0.8
B 1.25 0.8
• Result monitor A calculation: endpoint, air blanked, 510/700 nm
• Result monitor B calculation: endpoint, air blanked, 510/700 nm
• Sensitive to R2 probe-to-cuvette alignment and R2 probe mix.
• ACP is extremely unstable. Serum samples should be run immediately after collection if
possible. If this is not possible, the sample must be stabilized with an acid. Refer to the insert
sheet for further detail. QC product should be handled the same way, otherwise, you will get
low results. Acid tablets are available through SIGMA. One tablet to 1 mL of serum.
• Long R2 delivery time and long PHOT READ times. Instrument throughput will be reduced
when R2 delivery time is greater than 244 seconds and photometry read times are greater than
454 seconds.
Dimension® Chemistry D-00987 December 2000
Company Confidential ACTM-1
ACTM
Method Chemistry
First cuvette (blank)
Event # Event Delivery Volumes/Filters Mix
1 R1ARM (R1) 0 µL (H O) 150 µL N
2
2 SAMPLE 4 µL, (H O), 16 µL Y
2
3 R2ARM (R2) 80 µL, (R3) 80 µL, (H O) 100 µL Y
2
4 PHOT READ 600/700 nm
Total Cuvette Volume: 430 µL
Second cuvette (test)
Event # Event Delivery Volumes/Filters Mix
1 R1ARM (R1) 80 µL, (H O) 70 µL N
2
2 SAMPLE 4 µL (H O) 16 µL Y
2
3 R2ARM (R2) 80 µL, (R3) 80 µL, (H O) 100 µL Y
2
4 PHOT READ 600/700 nm
Total Cuvette Volume: 430µL
Method Specifics
• Endpoint, bichromatic 600/700 nm
• Type ‘c’ method
• Flex® type = 6 well, all liquid reagents
• Calibration type: linear
• Five unit negative bias versus aca® and TDx® due to calibrator differences
• Excellent precision performance (%CV) as compared to TDx®
• Results of approximate 30ug/mL can be obtained if there is no sample in cup, sample is not
delivered to the first cuvette (fibrin clot) or if water is tested for ACTM.
D-00987 December 2000 Dimension® Chemistry