Table Of ContentProceedings in Life Sciences
European Society for Comparative Physiology and Biochemistry
4th Conference, Bielefeld, September 8-11, 1982
Conference Organization
General Organizers
R. Gilles and H. Langer
Liege, Belgium/Bochum, FRG
Local Organizers
K. Immelman, E. Prove, and S. Sossinka
Bielefeld, FRG
Symposium Organizers
Intestinal Transport
M. Gilles-Baillien
Liege, Belgium
Hormones and Behaviour
J. Bal thazart
Liege, Belgium
Under the Patronage of
The Deutsche Forschungsgemeinschaft
The Department for Scientific Research of the
Bundesland Nordrhein Westfalen
The Paul-Martini-Stiftung der
Medizinisch Pharmazeutischen Studiengesellschaft
The University of Bielefeld
The University of Liege
The European Society for Comparative Physiology and Biochemistry
Intestinal Transport
Fundamental and Comparative Aspects
Edited by
M. Gilles-Baillien and R. Gilles
With 155 Figures
Springer-Verlag
Berlin Heidelberg New York Tokyo 1983
Dr. M. GILLES-BAILLIEN, Scientific Editor
Laboratory of General and
Comparative Biochemistry
University of Liege
17, Place Delcour
4020 Liege, Belgium
Professor Dr. R. GILLES, Coordinating Editor
Laboratory of Animal Physiology
University of Liege
22, Quai Van Beneden
4020 Liege, Belgium
ISBN -13: 978-3-642-69111-9 e-ISBN- 13: 978-3-642-69109-6
001: 10.1007/978-3-642-69109-6
Library of Congress Cataloging in Publication Data. Main entry under title: Intestinal transport. (proceedings
in life sciences) Lectures from a symposium held at a conference of the European Society for Comparative
Physiology and Biochemistry, Bielefeld, FRG, September 8-11, 1982. I. Intestinal absorption-Congresses.
2. Biological transport-Congresses. I. Gilles-Baillien, M. (Michelle), 1939-. II. Gilles, R. II. European
Society for Comparative Physiology and Biochemistry. IV. Series. QP156.I567 1983 599'.0132 83-4826
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Preface
The aim of this symposium was to provide a framework for fruitful discussion
on intestinal transport, not only for advanced scientists but also for younger
people starting in this field of research. Invited lectures, communications and
poster presentations were focused on four central themes, all treating the prop-
erties of the sole intestinal epithelium, deliberately leaving aside problems
dealing with more integrative functions of the whole intestine. The importance
of motility or blood circulation, for instance, is certainly capital in the overall
intestinal function, but these aspects by themselves deserve another meeting.
This volume has compiled the manuscripts of the invited lectures which sub-
stantially comprised the four sessions of the Symposium.
Part 1 is designed to emphasize actual knowledge of the transport of water,
inorganic as well as organic ions and molecules across the isolated intestinal
epithelium. An enormous wave of investigations has emerged from studies per-
formed with "Ussing chambers", which roused interest in studies on absorption
mechanisms and subsequently on secretory processes. This has triggered off a
trend to research on isolated cells as absorption and secretion are the main func-
tion of the different cell types constituting the intestinal epithelium. In this first
session not only the importance of the parallel arrangement of these different
cellular entities is stressed, but also the role played by the paracellular route.
Moreover, though the interference of unstirred layers, of the mucus coating,
and of the basement membrane are illustrated in this first part, there is undeni-
ably a gap in research at this level which has to be filled before the role of these
extracellular compartments in transport processes across the whole epithelium
can be fully assessed.
Part 2 reflects the present major interest in sophisticated studies performed
with vesicles obtained from purified brush-border and basolateral membranes
and designed to elucidate transport or carrier mechanisms at these two levels.
With this material appears growing information on the function of "carriers" at
the molecular level; the use of biochemical as well as biophysical technical
methods allows an approach to the complex relationship between the different
membrane components organized to perform exchanges of matter and energy
across these membranes.
Part 3 deals with some aspects of the regulation and control of intestinal
transport. The enormous amount of information presented by clinical studies
VI Preface
of human pathology on the intestinal function and on the therapeutical solu-
tions envisaged, has been and still is one of the major stimuli to the many pos-
sibilities and means of regulation of intestinal transport. Pioneer work in this
field is presented in the third section.
In part 4 our aim has been to arouse cross-reactions and mutual interest
between fundamentalists and comparatists. Indeed, from the many concepts of
intestinal transport processes which have appeared in the course of animal
evolution, we believe that a more comprehensive knowledge could be acquired.
We hope that this volume will be a valuable tool not only for young or
advanced scientists but also for students whishing to bring their knowledge of
progress and gaps in the field of intestinal transport up to date.
Liege, April 1983 M. GILLES-BAILLIEN
Contents
Introductory Survey
Contributions and Stimulus to Intestinal Transport Studies
K.A. Munday and J.A. Poat. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Part 1. From the Whole Epithelium to Isolated Cells
Routes of Water Flow Across the Intestine and Their Relationship to
Isotonic Transport
R.J. Naftalin and S. Tripathi . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 14
Absorption of Inorganic Ions and Short-Chain Fatty Acids in the Colon
of Mammals
W. v. Engelhardt and G. Rechkemmer . . . . . . . . . . . . . . . . . . . . . . .. 26
Cellular Aspects of Amino-Acid Transport
M.W. Smith, F.V. Sepulveda, and J.Y.F. Paterson. . . . . . . . . . . . . . .. 46
Statistical Analysis of Solute Influx Kinetics
J.W.L. Robinson, G. Van Melle, and S. Johansen. . . . . . . . . . . . . . . .. 64
Intestinal Secretion of Organic Ions
F. Lauterbach. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 76
Coupling Stoichiometry and the Energetic Adequacy Question
G. Kimmich. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 87
Several Compartments Involved in Intestinal Transport
M. Gilles-Baillien. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 103
VllI Contents
Part 2. Brush Border and Basolateral Membranes
Mechanisms of Sodium Transport Across Brush Border and Basolateral
Membranes
E.M. Wright, R.D. Gunther, J.D. Kaunitz, B.R. Stevens, V. Harms,
H.J. Ross, and R.E. Schell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 122
Transport of Inorganic Anions Across the Small Intestinal Brush Border
Membrane
H. Murer, J. Biber, V. Scalera, G. Cassano, B. Stieger, G. Danisi, B. Hild-
mann, G. Burckhardt, and H. Lucke . . . . . . . . . . . . . . . . . . . . . . . .. 133
Mechanisms of Sugar Transport Across the Intestinal Brush Border
Membrane
E. Brot-Laroche and F. Alvarado. . . . . . . . . . . . . . . . . . . . . . . . . .. 147
Mechanism of Active Calcium Transport in Basolateral Plasma Membranes
of Rat Small Intestinal Epithelium
C.H. Van Os and W.EJ.M. Ghijsen . . . . . . . . . . . . . . . . . . . . . . . . .. 170
The small Intestinal Na+, D-Glucose Cotransporter: a Likely Model
G. Semenza . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 184
Protein-Lipid Interactions and Lipid Dynamics in Rat Enterocyte Plasma
Membranes
Th.A. Brasitus .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 188
Part 3. Regulation of Intestinal Transport
Role of Cell Sodium in Regulation of Transepithelial Sodium Transport
K. Tumheim. . ..................................... " 200
Calcium Regulation of Intestinal Na and CI Transport in Rabbit Ileum
D.W. Powell and C.C. Fan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 215
Role of Calcium and Cyclic Nucleotides in the Regulation of Intestinal
Ion Transport
M.C. Rao and M. Field ................................ " 227
Neuro Hormonal Control of Intestinal Transport
L.A. Tumberg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 240
Hormone Regulation of Intestinal Calcium and Phosphate Transport:
Effects of Vitamin D, Parathyroid Hormone (PHI) and Calcitonine (CT)
T. Drueke and B. Lacour. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 249
Contents IX
Part 4. Comparative Aspects of Intestinal Transport
Comparative Aspects of Amino Acid Transport in Guinea Pig, Rabbit and
Rat Small Intestine
B.G. Munck ......................................... 260
Temporal Adaptation and Hormonal Regulation of Sodium Transport in
the Avian Intestine
E. Skadhauge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 284
Effect of Galactose on Intracellular Potential and Sodium Activity in
Urodele Small Intestine. Evidence for Basolateral Electrogenic Sodium
Transport
J.F. White and M.A. Imon. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 295
Transport of Ions and Organic Molecules in the Midgut of some Lepidop·
teran Larvae
S. Nedergaard. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 313
Electrical Phenomena in Fish Intestine
J.A. Groot, H. Albus, R. Bakker, J. Siegenbeek van Heukelom, and
Th. Zuidema. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 321
Intestinal Transport and Osmoregulation in Fishes
B. Lahlou. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 341
Biochemical Adaptation of Trout Intestine Related to Its Ion Transport
Properties. Influence of Dietary Salt and Fatty Acids, and Environmental
Salinity
C. Leray and A. Florentz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 354
Subject Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 369
List of Contributors
You will find the addresses at the beginning of the respective contribution
Albus, H. 321 Melle, G. Van 64
Alvarado, F. 147 Munck, B.G. 260
Biber, J. 133 Munday, K.A. 2
Bakker, R. 321 Murer, H. 133
Brasitus, Th.A. 188 Naftalin, R.J. 14
Brot-Laroche, E. 147 Nedergaard, S. 313
Burckhardt, G. 133 Os, C.H. Van 170
Cassano, G. 133 Paterson, J.Y.F. 46
Danisi, G. 133 Poat, J.A. 2
Driieke, T. 249 Powell,D.W. 215
Engelhardt, W. v. 26 Rao, M.C. 227
Fan, C.C. 215 Rechkemmer, G. 26
Field, M. 227 Robinson, J.W.L. 64
Florentz, A. 354 Ross, H.J. 122
Ghijsen, W.E.J .M. 170 Scalera, V. 133
Gilles-Baillien, M. 103 Schell, R.E. 122
Groot, J.A. 321 Semenza, G. 184
Gunther, R.D. 122 Sepulveda, F.V. 46
Harms, V. 122 Siegenbeek van Heukelom, J. 321
Hildmann, B. 133 Skadhauge, E. 284
Imon, M.A. 295 Smith, M.W. 46
Johansen, S. 64 Stevens, B.R. 122
Kaunitz, J.D. 122 Stieger, B. 133
Kimmich, G. 87 Tripathi, S. 14
Lacour, B. 249 Turnberg, L.A. 240
Lahlou, B. 341 Turnheim, K. 200
Lauterbach, F. 76 White, J.F. 295
Leray, C. 354 Wright, E.M. 295
Liicke, H. 133 Zuidema, Th. 321
