Impact of seed priming on antioxidant metabolism and yield of rice under water stress

262 Pages·2012·8.61 MB·English

Impact of seed priming on antioxidant
metabolism and yield of rice under
water stress

THESIS SUBMITTED IN PARTIAL FULFILMENT OF THE
REQUIREMENTS FOR THE DEGREE OF
Doctor of Philosophy
in
Plant Physiology

By
Bhawna Pant

Supervisor
Professor (Mrs.) Bandana Bose

DEPARTMENT OF PLANT PHYSIOLOGY
INSTITUTE OF AGRICULTURAL SCIENCES
BANARAS HINDU UNIVERSITY
VARANASI - 221005

I.D.No. PP-11065 2015 Enrolment No. 341674
Copyright © Institute of Agricultural Sciences,
Banaras Hindu University, Varanasi, India, 2015.
All rights reserved.
UNDERTAKING BY THE CANDIDATE
I, Bhawna Pant, I.D. No. PP-11065 and Enrollment number 341674 declares
that I have pursued my research work under the supervision of Prof. (Mrs.) Bandana
Bose from 31-10-2011 to 10-10-2015. I hereby supplicate my thesis entitled “Impact
of seed priming on antioxidant metabolism and yield of rice under water stress”.

Date:
Place: B.H.U., Varanasi (Bhawna Pant)
Annexure – E
[see clause XIII.2(b)(iii)]
CANDIDATES’S DECLARATION
I, Bhawna Pant, PP-11065, Enrolment Number 341674, certify that the work
embodied in this Ph.D. thesis is my own bonafide work carried out by me under the
supervision of Prof. (Mrs.) Bandana Bose for a period of three years eleven months
from 31-10-2011 to 10-10-2015 at Institute of Agricultural Sciences, Banaras Hindu
University. The matter embodied in this Ph.D. thesis has not been submitted for the
award of any other degree/diploma.
I declare that I have faithfully acknowledged, given credit to and referred to
the research workers wherever, their works have been cited in the text and the body of
the thesis. I further certify that I have not willfully lifted up some other’s work, para,
text, data, results, etc. reported in the journals, books, magazines, reports,
dissertations, thesis, etc., or available at web sites and included them in this Ph.D.
thesis and cited as my own work.
Date: ……………..
(Signature of the Candidate)
Place: B.H.U., Varanasi
(Bhawna Pant)

CERTIFICATE FROM SUPERVISOR/CO-SUPERVISOR
This is to certify that the above statement made by the candidate is correct to
the best of our knowledge.
…………………….
[Prof. (Mrs.) Bandana Bose]
(Supervisor)

(Signature of HOD/ Coordinator of the school with seal)
Annexure – F
[see clause XIII.1(c) and XIII.2(b)(iv)]
COURSE / COMPREHENSIVE EXAMINATION
COMPLETION CERTIFICATE
This is to certify that Mrs. Bhawna Pant, PP-11065 with Enrolment Number
341674, a bonafide Research Scholar of this Department, has successfully completed
the course work / Comprehensive Examination requirements which are the part of her
Ph.D. Programme.

Date: (Signature of the Head of the Department)
Place: B.H.U., Varanasi
Annexure – F
[see clause XIII.1(c) and XIII.2(b)(iv)]
PRE-SUBMISSION SEMINAR COMPLETION CERTIFICATE
This is to certify that Mrs. Bhawna Pant, PP-11065 with Enrolment number
341674, a bonafide Research Scholar of this Department, has successfully completed
the Pre-Submission Seminar on 09-09-2015 on the topic “Impact of seed priming on
antioxidant metabolism and yield of rice under water stress” which is a part of her
Ph.D. Programme.

Date: (Signature of the Head of the Department)
Place: B.H.U., Varanasi
Annexure – G
[see clause XIII.2(b)(v)]
COPYRIGHT TRANSFER CERTIFICATE
Title of the thesis : “Impact of seed priming on antioxidant metabolism
and yield of rice under water stress”
Candidate’s Name : Bhawna Pant

COPYRIGHT TRANSFER
The undersigned hereby assigns to the Banaras Hindu University all rights
under copyright that may exist in and for the above thesis submitted for the award of
the Ph.D. degree.

(Signature of the Candidate)

Notice: However, the author may reproduce or authorize others to reproduce
material extracted verbatim from the thesis or derivative of the thesis
for author’s personal use provided that the source and the university’s
copyright notice are indicated.
Impact of seed priming on antioxidant metabolism and yield of rice
under water stress

Thesis submitted in partial fulfillment of the requirements for
the award of the degree of
Doctor of Philosophy
in
Plant Physiology

2015

By
Bhawna Pant

APPROVED BY RESEARCH PROGRAMME COMMITTEE

Chairman Dr. (Mrs.) Bandana Bose
Professor and Head
Department of Plant Physiology

DRC Nominee Dr. Pravin Prakash
Associate Professor
Department of Plant Physiology

Internal Subject Expert Dr. P. Dwivedi
Professor
Department of Plant Physiology

External Subject Expert Dr. R. P. Singh
Professor
Department of Genetics and Plant Breeding

Dr. Avijit Sen
Professor and Head
Department of Agronomy

EXTERNAL EXAMINER
ACKNOWLEDGEMENT

Firstly, I bow my head with utmost reverence before the almighty "Lord
Kashi Vishwanath Ji" whose eternal blessing and invisible help has enable me to
accomplish my Ph.D. successfully.
Indeed, with knee down, I bow my head and offer flowers of reverence to
Bharat Ratna Mahamana Pandit Madan Mohan Malaviya, the founder of the
Banaras Hindu University for his lifetime sacrifice and efforts in establishing such a
great temple of learning for the cause of millions of students like me. In fact,
Mahamana’s life has genuinely inspired me to be a good human being.
I would like to express deep and sincere gratitude to my esteemed supervisor,
Dr. (Mrs.) Bandana Bose, Professor, Department of Plant Physiology, Institute of
Agricultural Science, Banaras Hindu University, for her meticulous guidance,
indelible inspiration, persistent encouragement, ingenious suggestions, mellifluous
nature and indefatigable attitude. Her wide knowledge and logical way of thinking
have been of great value for me.
I extend my indebtedness to Dr. (Mrs.) Bandana Bose, Professor and Head,
Department of Plant Physiology, Institute of Agricultural Science, Banaras Hindu
University, for providing all the necessary facilities, her discerning comments,
valuable suggestions, co-operations and helpful attitude towards me during the
course of investigation.
I wish to express my heartfelt and advent thank to the members of my
Research Programme Committee, Dr. Padmanabh Dwivedi, Professor, Department
of Plant Physiology Dr. Pravin Prakash, Assistant Professor, Department of Plant
Physiology, Dr. R. P. Singh, Professor Department of Genetics and Plant Breeding,
and Dr. A. Sen, Professor, Department of Agronomy, for their valuable suggestions
and liberal help rendered during the course of study and research work.
I deem it my privilege in expressing my gratefulness to my teachers Dr. J.P.
Srivastava, Professor, Dr. A. Hemantaranjan, Professor, Dr. (Mrs.) Vijai P.,
Assistant Professor, Dr. (Mrs.) Savita Jangde, Assistant Professor, Department of
Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University,
Varanasi.
I am also very thankful to Dr. R. P. Singh, Director, Institute of
Agricultural Sciences, for providing the polyhouse of Department of Genetics and
Plant Breeding, for doing all the pot experiments related to this work.
I express my warm full thanks to Dr. P. K. Singh, Professor, Department of
Genetics and Plant Breeding, Institute of Agricultural Sciecnes, in providing seeds,
to complete my research work.
I am also very thankful to all the non-teaching staff members of Department
of Plant Physiology, Institute of Agricultural Sciences, Banaras Hindu University,
Varanasi, for their manifold help throughout my course-cum research programme.
I pay my sweet and heartiest thanks to my generous seniors, Dr. Sananda,
Dr. Brijesh, Dr. Pradeep, Dr. Radha, Dr. Pratap, Dr. Surender, and Dr. Deepmala
who displayed profound sense of fraternity with elderly help and valuable
suggestions to complete this gigantic task.
I acknowledge my heartfelt appreciation to my batchmates, Ankita, Navin,
Preeti, Sunil, Akanksha, Maina, Anil and Dinesh, and also my sincere thanks to all
juniors, Mahesh, Krishna, Anuj, Sushmita, Prasann, Ajay, Sanjay, Asha, Afjal,
Rajesh, Ankita Preeti, Jhanvi, Shivani, Nitish, Vikrant, Saroj, Atar, Arvind,
Ravikant, Akhilesh and Anil for their affection and indispensable help, which made
my study period in the university cheerful.
Friends are always the key player during building of the foundation as they
mould us in a proper shape of personality, I would therefore like to extend my
heartfelt gratitude to my friends Ankita, Navin, Ratul, Kavita, Nutan and
Kanchan whose love and care, mischief and kind support did never let me feel away
of my family & all the golden moments shared with them made my stay at BHU a
memorable one.
Lastly I bow my head before my parents for their unending love, ever-
growing faith and inspiration, moral and spiritual support that prompted me to take
up and complete this course successfully. This would have been a dream without
love, care and support of my family. Their caring eyes and helping hands were
always there to share my ecstasies and sorrows. I am also thankful to my sisters
Smriti, Kanchan, Harshita and brother in laws Dinesh, Manish, Karan for cheering
me up always and their selfless sacrifice and moral support makes me enthusiastic
throughout my educational career, which enabled me to acquire the present
gratification. Dear most Parth, Deetya, and Pehu for their loving support.
I sincerely thank my new family, my mother-in-law, Harsha, which I never
felt as in-laws, for their love, support and motivation given to me and last but not
the least my father-in-law Lt. Shri Bholanath Joshi, whose eternal blessings and
unforgettable memories are always with me.
Last but not least, I must gave special thanks to my husband, Harsh,
without whose love, care, encouragement and patient, I would not have finished