Table Of ContentINTEBNATIONAL ENDOCEINOLOGIOAL SYMPOSIA
Immunological properties
of protein hormones
Proceedings of Meeting Organized under the
Auspices of Accademia Nazionale dei Lincei and
of Consiglio Nazionale delle Rieerche
Rome, June 1964
EDITED Β Y
FILIPPO POLVANI and PIERGIORGIO CROSIGNANI
Glinica Ginecologica delV Université,
Gentro di Endocrinologia Ginecologica, Gonsiglio Nazionale delle Rieerche
Milan, Italy
1966
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PREFACE
The immunology of protein hormones is a relatively new
field. The great majority of pertinent studies were performed
within the past ten years. It was not until immunologic methods
of high sensitivity had been developed, namely hemagglutination
and the application of radioactive isotopes as indices of antigen
antibody reaction that it became possible to consistently demonstra
te the antigenic nature of the protein hormones. Prior immuno
logic studies were conflicting, and inconsistent, in fact many
investigators believed that the protein hormones were not antigenic.
It is not surprising that difficulty had been encountered with
measurement and localization of hormones prior to the use of
immunologic methods. Investigators resorted to relatively simple
chemical and histochemical procedures or very drastic physiologic
experiments to obtain such information. The histochemical
methods employed in the past were dependent upon color changes
used and developed by dye chemists which were concerned with
reactions between small and relatively simple molecules. It is
not surprising that such techniques were lacking the specificity
required for the measurement and localization of the large and
complex protein hormones.
The measurement of protein hormones in biologic fluids until
the immunologic techniques were utilized, was therefore dependent
primarily upon biologic assays. These methods were very im
portant and are still essential to validate the immunologic assay
methods. They lack the sensitivity, precision, and reproducibility
of the immunologic methods.
It is not surprising, that with the immunologic methods so
much useful information has been obtained regarding quantitative
relationship of hormones in various biologic fluids. The use of
antisera to produce isolated hormone déficiences is a new and
important approach to the study of the mechanism of hormone
action. More recently studies have been initiated using immuno
logic techniques to localize hormones at the histologic level.
The Accademia JSTazionale dei Lincei and the Consiglio Nazio-
nale delle Rieerche have been of great service to the scientific
viii PREFACE
community by supporting this meeting which allowed the know
ledge that has been acquired in many of these areas to be sum
marized and documented. In particular, thanks are extended
to Professor Oalifano who has been the driving force behind this
meeting. Professors Polvani and Crosignani organized the meeting
and assumed the editorial responsibility necessary for the comple
tion of this volume. Professor Turnage and Mrs. Braun-Drach-
holz have helped in the preparation of this volume for publi
cation.
Finally, there is reason to be optimistic that the compilation
of the data and the ideas which are here presented will help for
mulate concepts and perhaps initiate additional experimentation
in this new and rapidly expanding field of immunoendocrinology.
EDWARD ROBERT ARQUILLA
LIST OF PARTECIPANTS
Honorary President: LUIGI CALIFANO, President of u Comitate di
Biologia e Medicina" del Consiglio Nazionale
delle Rieerche) Aceademico Nazionale deiLincei,
Borne, Italy.
General Chairman : EDWARD EOBERT ARQUILLA, Associate Pro
fessor of Pathology, University of California.
Secretaries : FILIPPO POLVANI, PIER GIORGIO CROSIGNANI,
Clinica Ginecologica delV Université, Milan-,
Centro di Endocrinologia Ginecologica, Consi
glio Nazionale delle Bicerehe., Italy.
* * *
* A. ACERBI, Laboratory di ricerca " Farmitalia Milan, Italy.
D. ANDREANI, Istituto di Patologia Medica delV Université, Borne,
Italy.
* E. R. ARQUILLA, Department of Pathology, University of Califor
nia, U.S.A.
* S. ASSA, Department of Medicine, Bogoff Medical Besearch In
stitute, Petah-Tiquah, Israel.
* G. BALDRATTI, Laboratori di ricerca " Farmitalia ", Milan, Italy.
* W. R. BUTT, Department of Clinical Endocrinology, and Midland
Hospital for Women, Birmingham, Great Britain.
L. CALIFANO, Istituto di Patologia Générale delV Université, Naples,
Italy.
C CASSANO, Istituto di Patologia Medica delV Université, Borne,
Italy.
C. CONTI, Istituto di Patologia Medica delV Université, Borne, Italy.
* P. 6. CROSIGNANI, Clinica Ginecologica dell} Université, Milan,
Italy.
* J. M. CROUGHS, Centraal Laboratorium van de Bloedtransfusie-
dienst van het Nederlandsche Boode Kruis, Amsterdam, The
Netherlands.
χ LIST OF PARTECIPANTS
A. DE BARBIERI, Istituto Sieroterapico Milanese, Milan, Italy.
G. DELLEPIANE, Clinica Ginecologica delV Université, Turin, Italy.
* H. DE RTJYTER, Department of Medicine, University Hospital,
Groningen, The Netherlands.
P. DONINI, Istituto Farmacologico " Serono Borne, Italy.
R. FRASER, Department of Medicine, Postgraduate Medical School,
London, Great Britain.
* C. GEMZELL, Akademiska Siukhuset.
* J. GIRARD, Basler Kinderspital, Basel, Switzerland.
* M. Y. GIRARD, Basler Kinderspital, Basel, Switzerland.
* F. C. GREENWOOD, Imperial Cancer Besearch Foundation, Divi
sion of Chemistry and Biochemistry, London, Great Britain.
* C. N. HALES, Department of Biochemistry, University of Cam
bridge, Great Britain.
* M. HARTOG, Department of Medicine, Postgraduate Medical
School, London, Great Britain.
R. HILL, World Health Organization, Geneva, Switzerland.
W. INGIULLA, Clinica Ginecologica delV Université, Florence, Italy.
F. INGRASSIA, Clinica Ginecologica delV Université, Florence, Italy.
* A. KOWADLO-SILBERGELD, Department of Medicine, Bogoff Me
dical Besearch Institute, Petah-Tiquah, Israel.
* Z. LARON, Pediatric Metabolic and Endocrine Service, Bogoff
Medical Besearch Institute, Petah-Tiquah, Israel.
* R. LEQUINE, Laboratory for Endocrinological Chemistry, Berg-
wegziekenhuis, Botterdam, The Netherlands.
* B. LUNENFELD, Endocrinology Besearch and Development Unit,
Government Hospital, Tel-HaShomer, Israel.
* G. MAGGI, Istituto di Patologia Medica, Pavia, Italy.
* D. MAINGAY, Centraal Labor atorium van de Bloedtransfusiedienst
van het Nederlandsche Boode Kruis, Amsterdam, The Nether
lands.
* C. MASALA, Istituto di Patologia Medica delV Université, Sassari,
Italy.
M. MASSAZZA, Clinica Ginecologica dell} Université, Milan, Italy.
LIST OF PARTECIPANTS
E. MAURIZIO, Clinica Ginecologica delVUniversité, Borne, Italy.
L. MOSCA, Istituto di Anatomia Patologica delV Université, Pavia,
Italy.
L. MOTTA, Istituto di Patologia Medica delV Université, Catania,
Italy.
M. NEGRI, Istituto di Patologia Medica delV Université, Borne, Italy.
Έ. PASETTO, Clinica Ginecologica delV] Université, Borne, Italy.
* A. G. E. PEARSE, Department of Pathology, Postgraduate Medical
School, London, Great Britain.
* G. PELLEGRINI, Istituto di Patologia Medica delV Université,
Pavia, Italy.
* F. POLVANI, Clinica Ginecologica delV Université, Milan, Italy.
* C. H. READ, Department of Pediatrics, University Hospital,
Iowa, U.S.A.
* T. RODARI, Istituto di Patologia Medica, Pavia, Italy.
* U. ROSA, "Sorin", Saluggia, Italy.
V. SCAFFIDI, Istituto di Patologia Medica, Messina, Italy.
* SCHOPMAN W., Laboratory for Endocrinological Chemistry, Berg-
wegziekenhuis, Botterdam, The Netherlands.
* U. SERAFINI, Istituto di Patologia Medica delV Université, Sas
sari, Italy.
R. SIMONETTA, Clinica Ostetrica Ginecologica, Turin, Italy.
* G. SPECCHIA, Istituto di Patologia Medica delV Université, Pavia,
Italy.
* O. SPERLING, Department of Medicine, Bokoff Medical Besearch
Institute, Petah-Tiquah, Israel.
* G. K. SUCHOWSKY, Laboratory di ricerca " Farmitalia ", Milan,
Italy.
A. TESAURO, Clinica Ginecologica delV Université, Naples, Italy.
* G. TORRIGIANI, Istituto di Patologia Medica delV Université,
Sassari, Italy.
* J. L. TOUBER, Centraal Laboratorium van de Bloedtransfusie-
dienst van het Nederlandsche Boode Kruis, Amsterdam, The
Netherlands.
xii LIST OF PARTECIPANTS
L. TRINKA, World Health Organization, Geneva, Switzerland.
* M. VEST, Basler Kinderspital, Basel, Switzerland.
* L. WIDE, Department of Obstetrics and Gynaecology, University
Hospital, Uppsala, Sweden.
V. ZAMBOTTI, Istituto di Ghimica Biologica, Université, Milan,
Italy.
Participants who contributed papers.
OPENING ADDRESS
L. CALIF AN ο
On behalf of the National Research Council I warmly wel
come delegates to this Symposium on the immunology of protein
hormones.
Today immunization phenomena in components peculiar to
the body have extensions undreamt of in the past. They are
increasingly enlarging our knowledge of this subject, to such an
extent that an important branch of pathology—immunopathology—
has already developed. It must also be stated that in replacement
therapy, hormones of exogenous origin are necessarily used, some
of which have similar but not identical composition to that of the
human hormone. They are, therefore, in a position to cause the
onset of immunizing phenomena which, in some cases, annul the
specific effect and in others lead to hypersensitivity and also,
because of the formation of immune complexes, cause even mark
ed damage.
I therefore enthusiastically welcomed the proposal of some
colleagues as to the possibility of holding a Symposium at a high-
scientific level, such as the present, on the immunological proper
ties of protein hormones, and I then formally proposed it to the
Accademia dei Lincei and to the National Research Council, that
is to the two most important cultural bodies in Italy. Both accepted
my proposal and therefore, also in the name of all present, I wish
to express my warmest gratitude to them for making this Sym
posium possible.
The sessions will be held in the pleasant hall of the Farnesina,
rich in history, facing Palazzo Corsini, which is the more traditional
and larger headquarters of the Academy.
I wish to thank the National Research Council, always so
ready to support any initiative aimed at promoting progress and
studies in Italy and also contact with foreign research-workers,
for their kindness in providing the necessary financial aid to carry
out this work.
Warmest and special thanks to all those foreign colleagues
who have come from far-distant countries to attend this Meeting;
2 L. CALIFANO
to Mr. Gianni, the Chancellor of the Academy, Mr. Roglia and
the Secretaries of the Meeting for the organization.
I shall now ask Professors Massazza and Gemzell to come
to the Chairman's table and Prof. Arquilla to open the Meeting
with his introductory speech.
