Immunochemical Techniques Part I: Hybridoma Technology and Monoclonal Antibodies

1008 Pages·1986·96.33 MB·English

by  John J. Langone

Contributors to Volume 121
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PAUL G. ABRAMS (10), NeoRx Corporation, Diseases, Centers for Disease Control,
Seattle, Washington 91189 Atlanta, Georgia 30333
W. STEVEN ADAIR (42), Department of Biol- TREBOR J. BJERCKE (54), Department of
ogy, Washington University, St. Louis, Medicine, Baylor College of Medicine,
Missouri 63130 Houston, Texas 03077
GUIDO ANTON! (16), Sclavo Research Cen- CLARE BLESSlNGER (41), Department of
ter, 53100 Siena, Italy Rheumatic Diseases, Hospital for Joint
DORETTA ARMELLINI (16), Sclavo Research Diseases, Mount Sinai Medical School,
Center, 53100 Siena, Italy New York, New York 30001
LEONIE K. ASHMAN (48), Department of TIMOTHY BLOCK (73), Department of Mi-
Microbiology and Immunology, The Uni- crobiology, Jefferson Medical College,
versity of Adelaide, Adelaide 5000, Aus- Philadelphia, Pennsylvania 19107
tralia ALBERT BOEY~ (30), Department of Micro-
M. ZOUHAIR ATASSI (8), Marrs McLean De- biology and Hygiene, Vrije Universiteit
partment of Biochemistry, Baylor College Brussel, B-1090 Brussels, Belgium
of Medicine, Houston, Texas 03077 D. J. BOLT (40), Reproduction Laboratory,
ROBERT C. ATKINS (78), Department of Ne- Animal Science Institute, Agricultural
phrology, Prince Henry's Hospital, Mel- Research Service, Beltsville Agricultural
bourne, Victoria 3004, Australia Research Center, United States Depart-
ment of Agriculture, Beltsville, Maryland
M. t. BANQUERIGO (28), The Johns
50702
Hopkins Oncology Center, The Johns
Hopkins University School of Medicine, BARBARA D. BOSS (3), Developmental Neu-
Baltimore, Maryland 50212 robiology Laboratory, The Salk Institute
FRANK BATTYE (71), Flow Cytometry Labo- for Biological Studies and Clayton Foun-
ratory, The Walter and Eliza Hall Insti- dation for Research-California Division,
tute of Medical Research, The Royal Mel- San Diego, California 6129-83129
bourne Hospital, Parkville, Victoria 3050, JOHN M. BOSWORTH, JR. (51), Department
Australia of Pediatrics, Uniformed Services Uni-
HERV,~ BAZIN (19, 59, 61, 63), Experimental versity of the Health Sciences, F. Edward
Immunology Unit, Faculty of Medicine, H(bert School of Medicine, Bethesda,
University of Louvain, B-1200 Brussels, Maryland 9974-41802
Belgium MARK BOTHWELL (73), Department of
ROSALIE BER (35), Rappaport Family Insti- Physiology and Biophysics, University of
tute for Research in the Medical Sci- Washington, School of Medicine, Seattle,
ences, Faculty of Medicine, Technion-ls- Washington 59189
rael Institute of Technology, Haifa ,69013 CLAUDE BOUCHEIX (55), INSERM U.253,
Israel H6pital Paul Brousse, 94800 ViUejuif,
WILLIAM F. BIBB (38), Division of Bacterial France
xiii
xiv CONTRIBUTORS TO VOLUME 121
REGID)~R W. BRAUN (70), Institutfiir Virus- versity School of Medicine, Baltimore,
forschung, Deutsches Krebsforschungs- Maryland 50212
zentrum, D-6900 Heidelberg, Federal Re- BRIAN R. CLARK (43), Division of Pediat-
public of Germany rics, City of Hope National Medical Cen-
SAMOHT BRODIN (25), Department of -uT ter, Duarte, California 01019
mor Immunology, The Wallenberg Labo- M. R. CLARK (52), Department of Pathol-
ratory, University of Lund, S-220 07 ogy, University of Cambridge, Cam-
Lund, Sweden
bridge 2BC 1QP, England
YHTOMIT L. SKOORB (58), Chromatography KCIRTAP H. CLEVELAND (50), Department
Business Unit, Bio-Rad Laboratories, of Ophthalmology, University of Califor-
Richmond, California 10849 nia, San Diego, Veterans Administration
MCKAY BROWN (29), Department of Micro- Medical Center, San Diego, California
biology and Immunology, Oregon Health 16129
Sciences University, Portland, Oregon W. Louis CLEVELAND (9), Department of
10279
Microbiology, Columbia University, New
CLAUDINE BRUCK (56), Department of Pa- York, New York 23001
thology, Harvard Medical School, Bos-
DAVID REHCLOC (76), Laboratory of Tumor
ton, Massachusetts ,51120 Department of Immunology and Biology, National Insti-
Medicine, New England Medical Center, tutes of Health, National Cancer Insti-
Boston, Massachusetts 02110, and La-
tute, Bethesda, Maryland 29802
boratoire de Chimie Biologique, Depart-
NASUS P. C. COLE (ll, 12), Department of
ment de Biologie Moleculaire, Universite
Microbiology and Immunology, Queen's
Libre de Bruxelles, B-1640 Rhode-St-Gen-
University, Kingston, Ontario K7L 3N6,
ese, Belgium
Canada, and Ontario Cancer Treatment
ABIGAIL BUENAFE (29), Department of Mi- and Research Foundation, Kingston Re-
crobiology and Immunology, Oregon gional Centre, Kingston, Ontario K7L
Health Sciences University, Portland, -rO 2V7, Canada
egon 10279
YRRAB S. COLLER (37), Division of Hema-
PAUL A. BUNN, JR. (65), Division of Medi- tology, State University of New York at
cal Oncology, University of Colorado Stony Brook, Stony Brook, New York
Health Sciences Center, Denver, Colo- 49711
rado 26208
YRALIH A. COLLER (37), Harvard~Radcliffe
TTOCS W. BURCHIEL (57), College of Phar- College, Cambridge, Massachusetts
macy, The University of New Mexico, Al- 83120
buquerque, New Mexico 13178
DAWN E. COLWELL (5), Department of Mi-
JENG-CHYH CHEN (58), Chromatography crobiology, University of Alabama at Bir-
Business Unit, Bio-Rad Laboratories, mingham, Birmingham, Alabama 49253
Richmond, California 10849
EVERLY CONWAY ED MACARIO (49),
MAURIZIO CIANFRIGLIA (16), Laboratorio Wadsworth Center for Laboratories and
di Biologia Cellulare, Istituto Superiore di Research, New York State Department of
Sanita', 16100 Roma, Italy Health, Albany, New York 10221
DANIEL R. ACCOIC (54), Laboratorio de Re- ESIO3(NARF TNOMROC (19, 59, 61), Experi-
nOiccudorp y Lactancia ( LARLAC), Con- mental Immunology Unit, Faculty of
sejo Nacional de Investigaciones Cientifi- Medicine, University of Louvain, B-1200
cas y Tdcnicas (CONICET), Mendoza Brussels, Belgium
5500, Argentina
TREBOR S. NAMSSIRC (15), Department of
CURT I. CIVIN (28), The Johns Hopkins -nO Anatomy, Medical College of Ohio,
cology Center, The Johns Hopkins Uni- Toledo, Ohio 99634
CONTRIBUTORS TO VOLUME 121 XV
OLRAC M. CROCE (11), The Wistar Institute tutes of Health, National Cancer Insti-
of Anatomy and Biology, Philadelphia, tute, Bethesda, Maryland 29802
Pennsylvania 40191 PETER L. EY (48), Department of Microbiol-
A. C. CUELLO (17), Department of Pharma- ogy and Immunology, The University of
cology and Therapeutics, Faculty of Med- Adelaide, Adelaide 5000, Australia
icine, McGill University, Montreal, Que- S. FAZEKAS ED ST.GROTH (33), Basel Insti-
bec H3G 1 ,6Y Canada tute for Immunology, CH-4005 Basel,
JOHN M. DAVIS (27), Division of Immunol- Switzerland
ogy, Department of Pathology, Univer- K. A. FOON (10), Division of Hematology
sity of Cambridge, Addenbrooke' s Hospi- and Oncology, Simpson Memorial Re-
tal, Cambridge CB2 2QQ, England search Institute, University of Michigan,
C. J. DEAN (6), Section of Tumour Immu- Ann Arbor, Michigan 48109
nology, Institute of Cancer Research, STEVEN K. H. FOUNG (13), Department of
Surrey SM2 5PX, England Pathology, Stanford University School of
LIEVE DE CLERCQ (19, 59, 61), Experimen- Medicine, Stanford University Medical
tal Immunology Unit, Faculty of Medi- Center, Stanford, California 50349
cine, University of Louvain, B-1200 Brus- KE~ITNARF FRAN~K (23, 60), Department of
sels, Belgium Membrane Biochemistry, Institute of Mo-
JEFFREY A. DREBIN (56), Department of Pa- lecular Genetics, Czechoslovak Academy
thology, Harvard Medical School, Bos- of Sciences, CS-142 20 Prague 4, Czecho-
ton, Massachusetts 02115, and Depart- slovakia
ment of Medicine, New England Medical NAI H. FRAZER (71), Division oflmmunol-
Center, Boston, Massachusetts 02110 ogy, Department of Medicine, University
KENDRA B. EAGER (7), The Wistar Institute of Queensland, Princess Alexandra Hos-
of Anatomy and Biology, Philadelphia, pital, Brisbane, Queensland 4102, Aus-
Pennsylvania 40191 tralia
WILLIAM C. EBY (74), Department of Pa- J. GHEUENS (39), Laboratory of Neuropa-
thology and Microbiology, Loma Linda thology, Born-Bunge Foundation, Un-
University, Loma Linda, California 05329 iversitaire Instelling Antwerpen, B-2610
PAUL H. EHRLICH (66), Monoclonal Anti- Wilrijk, Belgium
body Department, Sandoz Research Insti- SAMOHT J. GILL Ill (36), Department of Pa-
tute, Sandoz, Inc., East Hanover, New thology, University of Pittsburgh, School
Jersey 07936, and Department of Obstet- of Medicine, Pittsburgh, Pennsylvania
rics and Gynecology, University of Medi- 16251
cine and Dentistry of New Jersey, Pisca- STEPHEN GINN (15), Department of Anat-
taway, New Jersey 08854 omy, Medical College of Ohio, Toledo,
EDGAR G. ENGLEMAN (13), Department of Ohio 43699
Pathology, Stanford University School of KRAM C YSSALG (50), Department of Medi-
Medicine, Stanford University Medical cine, University of California San Diego
Center, Stanford, California 50349 Cancer Center, University of California,
DRANREB F. ERLANGER (9), Department of San Diego, La Jolla, California 39029
Microbiology, Columbia University, New CORINNE GLINEUR (56), Laboratoire de
York, New York 23001 Chimie Biologique, Department de Biolo-
DANIEL P. ESKINAZI (74), Division of Re- gie Moleculaire, Universite Libre de
search Grants, National Institutes of Bruxelles, B-1640 Rhode-St-Genese,
Health, Bethesda, Maryland 29802 Belgium
JOSE ESTEBAN (76), Laboratory of Tumor L. MICHAEL GLODE (67), Division of Medi-
Immunology and Biology, National Insti- cal Oncology, Department of Medicine,
xvi CONTRIBUTORS TO VOLUME 121
University of Colorado Health Sciences State Department of Health, Albany,
Center, Denver, Colorado 26208 New York 10221
DRAHCIR A. YBSDLOG (21, 40, 75), Depart- ROTCEH SANILAS-ZERAUJ (58), Chromatog-
ment of Biology, Amherst College, raphy Business Unit, Bio-Rad Laborato-
Amherst, Massachusetts 20010 ries, Richmond, California 10849
F. CARL GRUMET (13), Department of Pa- OIHSOT KAMIYA (77), Division of Cellular
thology, Stanford University School of Immunology, Cell-Engineering Research
Medicine, Stanford University Medical Center, Osaka University, Suita, Osaka
Center, Stanford, California 50349 565, Japan
TREBLA J. GUIDRY (21), Milk Secretion and H. W. D. KATINGER (32), Institute of Ap-
Mastitis Laboratory, Animal Science In- plied Microbiology, University of Agricul-
stitute, Agricultural Research Service, ture, A-I190 Vienna, Austria
United States Department of Agriculture, BENNETT KAUFMAN (75), Department of
Beltsville, Maryland 50702
Biologics Research, Walter Reed Army
L. A. GYURE (6), Section of Tumour Immu- Institute of Research, Walter Reed Army
nology, Institute of Cancer Research, Medical Center, Washington, D.C.
Surrey SM2 5PX, England 0015-70302
J. G. HALL (6), Section of Tumour Immu- IKUYOMOT OTOMAWAK (22), Department of
nology, Institute of Cancer Research, Biochemistry, Okayama University Den-
Surrey SM2 5PX, England tal School, Okayama City 700, Japan
WAYNE W. KCOCNAH (78), Department of REGOR H. KENNETT (7), Department of Hu-
Pathology, Brigham and Women's Hospi- man Genetics, School of Medicine, Uni-
tal and Harvard Medical School, Boston, versity of Pennsylvania, Philadelphia,
Massachusetts 51120 Pennsylvania 40191
IKUYIHSOY HASHIMOTO (77), Department JAN KOVA~ (23), Department of Membrane
of Hygienic Chemistry, Pharmaceutical Biochemistry, Institute of Molecular -eG
Institute, ukohS~T University, Aobayama, netics, Czechoslovak Academy of Sci-
Sendai 980, Japan ences, CS-142 20 Prague ,4 Czechoslo-
DRAHCIR HAWKES (46), Department of Bio- vakia
chemistry and Laboratory of Nearobiol- DANUTA ROBZOK (11, 12), ehT Wistar Insti-
ogy, Laval University, Quebec City, Que- tute of Anatomy and Biology, Philadel-
bec, Canada phia, Pennsylvania 40191
SALOHCIN J. DAARNEGOOH (34), Depart- AICIRTAP KRIEF (55), INSERM U.253, Hf-
ment of Biochemistry, La Trobe Univer- pital Paul Brousse, 94800 Villejuif ,
sity, Bundoora, Victoria 3083, Australia France
KENNETH W. HUNTER, JR. (51), Depart- GUNTHER LEM~tK (70), Abteilung Virusfors-
ments of Pediatrics and Preventive Medi- chung, Zentrum der Hygiene, D-6000
cine~Biometrics, Uniformed Services Frankfurt am Main, Federal Republic of
University of the Health Sciences, F. Germany
Edward H~bert School of Medicine, Be- UDO KUMMER (64), Department of Immu-
thesda, Maryland 9974-41802 nology, Institute of Hematology, Gesell-
SEMAJ P. JAKWAY (45), Department of Mi- schaft fiir Strahlen- und Umweltfors-
crobiology and Immunology and the chung m.b.H., D-8000 Munich 2, Federal
George W. Hooper Foundation, Univer- Republic of Germany
sity of California, San Francisco, San SAMOHT J. KUNICKI (67), Platelet Research
Francisco, California 22149 Laboratory, The Blood Center of South-
TREBOR J. JOVELL (49), Wadsworth Center eastern Wisconsin, Milwaukee, Wiscon-
for Laboratories and Research, New York sin 33235
CONTRIBUTORS TO VOLUME 121 xvii
HEINZ W. KUNZ (36), Department of Pa- thology, Institute of Psychiatry, London
thology, University of Pittsburgh, School SES 8AF, England
of Medicine, Pittsburgh, Pennsylvania KCIRTAP MANOUVRIEZ (59), Experimental
16251 Immunology Unit, Faculty of Medicine,
ATIREHGRAM LAFATA (16), Sclavo Re- University of Louvain, B-1200 Brussels.
search Center, 53100 Siena, Italy Belgium
EDMUNDO LAMOYi (62), Laboratory of Im- J. KOORBRAM (72), Department oflmmuno-
munology, National Institute of Allergy biology, School of Medicine, University
and Infectious Diseases, National Insti- of Auckland, Auckland, New Zealand
tutes of Health, Bethesda, Maryland OMISSAM MARIANI (16), Sclavo Research
29802 Center, 53100 Siena, Italy
RICHARD D. LANE (15), Department of ASILANNA ENOSSAM (16), Sclavo Research
Anatomy, Medical College of Ohio, Center, 53100 Siena, Italy
Toledo, Ohio 43699
SUZANNE M. MICHALEK (5), Department of
JOHN J. LANGONE (81), Department of Med- Microbiology, University of Alabama at
icine, Baylor College of Medicine, Hous- Birmingham, Birmingham, Alabama
ton, Texas 03077 49253
P. M. LANSDORP (80), Terry Fox Labora- K, F. MILLER (40), Reproduction Labora-
tory, British Columbia Cancer Research tory, Animal Science Institute, Agricul-
Centre, Vancouver, British Columbia tural Research Service, Beltsville Agricul-
VSZ lL3, Canada tural Research Center, United States
EROT LINDMO (65), Department of Biophys- Department of Agriculture, Beltsville,
ics, Norsk Hydro's Institute for Cancer Maryland 50702
Research, Oslo 3, Norway NOTSGNIK MILLS (69), Division of Immu-
DANIEL REKCOL (47), Laboratory oflmmu- nology, National Institute for Medical
nogenetics, CNRS, CSEAL, and Univer- Research, London NW7 1AA, England
sity of Orleans, 45067 Orleans Cedex 2, C. MILSTEIN (17), Medical Research Coun-
France cil, Laboratory of Molecular Biology,
OTREBLA J. L. MACARIO (49), Wadsworth Cambridge CB2 2QH, England
Center for Laboratories and Research, DUOSSAM MIRSHAHI (55), Service de M~de-
New York State Department of Health, cine Interne et Oncologie, HOtel Dieu,
Albany, New York 10221 40057 Paris, France
NOD B. MCCLURE (22), Division of Molecu- ARDNERIHD N. MISRA (36), Department of
lar and Cellular Biology, Eli Lilly and Pathology, University of Pittsburgh,
Company, Indianapolis, Indiana 46285 School of Medicine, Pittsburgh, Pennsyl-
D. E. MCFARLIN (39), Neuroimmunology vania 16251
Branch, National Institutes of Neurologi- GIUSEPPE A. MOLINARO (74), Department
cal and Communicative Disorders and of Pathology and Microbiology, Loma
Stroke, Bethesda, Maryland 50202 Linda University, Loma Linda, California
YRREJ R. MCGHEE (5), Department of Mi- 05329
crobiology, University of Alabama at Bir- AICIRTAP K. A. MONGINI (41), Department
mingham, Birmingham, Alabama 35294 of Rheumatic Diseases, Hospital for Joint
NAI R. MACKAY (71), Clinical Research Diseases, Mount Sinai Medical School,
Unit, The Walter and Eliza Hall Institute New York, New York 30001
of Medical Research, The Royal Mel- TREBOR R. YREMOGTNOM (67), Hemostasis
bourne Hospital, Parkville, Victoria ,0503 Research Laboratory, The Blood Center
Australia of Southeastern Wisconsin, Milwaukee,
DAVID MALE (53), Department of Neuropa- Wisconsin 33235
xviii CONTRIBUTORS TO VOLUME 121
ESIO~tNARF XENROM (76), Laboratory of -uT RIvo PRESENTINI (16), Sclavo Research
mor Immunology and Biology, National Center, 53100 Siena, Italy
Institutes of Health, National Cancer In- HTERAG ECYRP (53), Department of Immu-
stitute, Bethesda, Maryland 29802 nology, Middlesex Hospital Medical
K. HCABSOM (32), Pure and Applied Bio- School, London W1P 9PG, England
chemistry, University of Lund, S-221 0O JANET H. RANSOM (24), Bionetics Re-
Lund, Sweden search, Inc., Rockville, Maryland 05802
EVEIVENEG MOTTA (47), Laboratory oflm- REHPOTSIRHC L. READING (2), Depart-
munogenetics, CNRS, CSEAL, and Uni- ments of Tumor Biology and Hematology,
versity of Orleans, 45067 Orleans Cedex ehT University of Texas System Cancer
2, France Center, M. D. Anderson Hospital & -uT
mor Institute, Houston, Texas 03077
WILLIAM R. MOYLE (66), Department of
Obstetrics and Gynecology, University of NIVRAM B. GREBNETTIR (29), Department
Medicine and Dentistry of New Jersey, of Microbiology and Immunology, Ore-
Piscataway, New Jersey 45880 gon Health Sciences University, Port-
land, Oregon 10279
GARTH L. NICOLSON (68), Department of
Tumor Biology, The University of Texas JOHN C. RODER (11, 12), Division of Molec-
System Cancer Center, M. D. Anderson ular Immunology and Neurobiology,
Hospital & Tumor Institute, Houston, Mount Sinai Medical Research Institute,
Texas 03077 Toronto, Ontario M5G 1X5, Canada
K. NILSSON (32), Pure and Applied Bio- CLAUDE ROSENFELD (55), INSERM ,352.U
chemistry, University of Lund, S-221 0O H6pital Paul Brousse, 94800 Villejuif,
Lund, Sweden France
SAAD A. NOEMAN (36), Department of Bio- YERFFEJ L. ROSSlO (10), Program Re-
chemistry (Immunology), Faculty of sources, Inc., National Cancer Institute,
Medicine, University of Tanta, Tanta, Frederick Cancer Research Facility,
Egypt Frederick, Maryland 10712
TRANNEL NOSSLO (25), Department of Med- J. XUAESSUOR (63), Unit( 904 du CNRS, In-
icine A, State University Hospital, -oC stitut de Recherches sur le Cancer, 54095
penhagen 2100, Denmark Lille Cedex, France
LARS 0STBERG (18), Pharmaceutical Divi- R. TSOVERP-XUAESSUOR (63), Unit( 904 du
sion, Sandoz Research Institute, Sandoz, CNRS, lnstitut de Recherches sur el
Inc., East Hanover, New Jersey 63970 Cancer, 59045 Lille Cedex, France
GARY S. OTT (58), Chromatography Busi- OCSICNARF SANCHEZ-MADRID (20), Servi-
ness Unit, Bio-Rad Laboratories, Rich- cio de lnmunologia, Hospital de al Prin-
mond, California 10849 cesa, 28006 Madrid, Spain
JEAN-YVES TORREP (55), Service de M(de- NODROG H. SATO (22), W. Alton Jones Cell
cine Interne et Oncologie, H6tel Dieu, Science Center, Lake Placid, New York
40057 Paris, France 64921
YELREVEB L. PIKE (31), Cellular Immunol- J. DENRY SATO (22), .W Alton Jones Cell
ogy Unit, The Walter and Eliza Hall Insti- Science Center, Lake Placid, New York
tute of Medical Research, ehT Royal Mel- 64921
bourne Hospital, Parkville, Victoria ,0503 W. RERIEHCS (32), Sandoz Forschungsinsti-
Australia tut Gmblt, A-1235 Vienna, Austria
DANIEL ELLETETROP (56), Laboratoire de JOHN E. SHIVELY (43), Division of Immu-
Microbiologie, Faculte des Sciences nology, Beckman Research Institute of
Agronomiques, B-5800 Gembloux, Bel- the City of Hope, Duarte, California
gium 01019
CONTRIBUTORS TO VOLUME 121 XiX
MICHAEL E. SIERZEGA (11), The Wistarln- TIMOTHY V. UPDYKE (68), Department of
stitute of Anatomy and Biology, Philadel- Tumor Biology, The University of Texas
phia, Pennsylvania 40191 System Cancer Center, M. D. Anderson
HANS OLov SJOGREN (25), Department of Hospital & Tumor Institute, Houston,
Tumor Immunology, ehT Wallenberg Texas 03077
Laboratory, University of Lund, S-220 07 ACINOREV NAV HEYNINGEN (44), Medical
Lund, Sweden Research Council Clinical and Population
M. SPITZ (4), Department of Immunology, Cytogenetics Unit, Edinburgh EH4 2XU,
National Institute for Biological Stan- Scotland
dards and Control, Hampstead, London P. NAV MOURIK (14), Central Laboratory of
NW3 6RB, England the Netherlands Red Cross Blood Trans-
YHTOMIT A. SPRINGER (20), Laboratory of fusion Service, 1006 AD Amsterdam, ehT
Membrane Immunochemistry, Dana-Far- Netherlands
bet Cancer Institute, Harvard Medical
HELEN VAN VUNAKIS (81), Department of
School, Boston, Massachusetts 51120
Biochemistry, Brandeis University, Wal-
S. NARAMUKIRS (21), Department of Veteri- tham, Massachusetts 45220
nary Science, University of Nebraska,
CHRISTOPH WAGENER (43), Abteilung
Lincoln, Nebraska 5090-38586
Klinische Chemie, Universitiit Pathobio-
YRRAL H. STANKER (58), Biomedical Sci- chemie der Mechizinischen, Fakult~it der
ences Division, Lawrence Livermore Na- RWTH Aachen, D-5100 Aachen, Federal
tional Laboratory, University of -rofilaC Republic of Germany
nia, Livermore, California 05549
DAVID E. WELLS (38), Department of Mi-
H. C. NOSNEVETS (10), Biological Response
crobiology, University of Alabama at Bir-
Modifiers Program, National Cancer In-
mingham, Birmingham, Alabama 49253
stitute, Frederick Cancer Research Facil-
ity, Frederick, Maryland 10712 REGINE J. TDUOWRETSEW (l), Laboratory
for Electron Microscopy, University of
J. M. STYLES (6), Section of Turnout Immu-
Leiden, 3332 AA Leiden, ehT Netherlands
nology, Institute of Cancer Research,
Surrey SM2 5PX, England PAMELA L. WITTE (35), Immunobiology
MINORU SUGAWARA (77), Division of Bio- and Cancer Research Program, Okla-
chemistry, Japan Roche Company Insti- homa Medical Research Foundation,
tute, Kajiwara, Kamakura 247, Japan Oklahoma City, Oklahoma 40137
M. R. SURESH (17), Summa Biomedical A. W. WOGNUM (80), Central Laboratory of
Canada Ltd. and Faculty of Pharmacy, the Netherlands Red Cross Blood Trans-
University of Alberta, Edmonton G6T fusion Service and Laboratory for Experi-
2N8, Canada mental and Clinical Immunology, Univer-
SHINYA SUZUKI (77), Department of Hy- sity of Amsterdam, 1006 AD Amsterdam,
gienic Chemistry, Pharmaceutical Insti- The Netherlands
tute, T6hoku University, Aobayama, Sen- REHPOTSIRHC J. WRAIGHT (34), Department
dai 980, Japan of Biochemistry, La Trobe University,
BRYAN M. TURNER (79), Department of Bundoora, Victoria 3083, Australia
Anatomy, The University of Birmingham, W. P. REKAMELUEZ (14, 80), Central Labo-
Medical School, Birmingham 51B 2TJ, ratory of the Netherlands Red Cross
England Blood Transfusion Service and Labora-
P. A. UNDERWOOD (26), CS1RO, Division tory for Experimental and Clinical Immu-
of Molecular Biology, North Ryde, New nology, University of Amsterdam, 6001
South Wales 2113, Australia AD Amsterdam, The Netherlands
Preface
Since hybridoma technology for the purpose of eliciting monoclonal
antibodies of predefined specificity was introduced by K6hler and Mil-
stein in 1975, it has become a mainstay in most laboratories that utilize
immunochemical techniques to study problems in basic, applied, or clini-
cal research. In simplest terms, the steps leading to the production of
hybridomas and their monoclonal antibody products suitable for a specific
purpose remain the same: immunization, cell fusion, cell growth and clon-
ing, propagation of stable hybridomas, and screening for antibody speci-
ficity and functional activity. The process is labor intensive and several
months may be required before appropriate cell lines are obtained and
monoclonal antibodies are fully characterized. It is not surprising that the
widespread use of monoclonal antibodies has led to the rapid develop-
ment of new techniques to improve the efficiency, sensitivity, and sim-
plicity of the individual steps.
This volume is divided into three sections that cover the various as-
pects of producing and maintaining hybridomas as well as of detecting,
characterizing, and utilizing monoclonal antibodies. It is not possible to
cover every development in methodology. Therefore, we have included
established techniques as well as selected newly developed approaches
that we feel would be especially useful because of their general applicabil-
ity. We have also included a list of articles from other volumes of Meth-
ods in Enzymology which are relevant to topics covered in this volume.
We have cross-referenced these articles for the convenience of the reader
and to avoid unwarranted duplication. As the field of hybridoma technol-
ogy undergoes further development, publication of supplemental volumes
will be considered.
We are grateful for the sound advice and constructive suggestions
from Nathan Kaplan and Sidney Colowick during the preparation of this
volume. The recent passing of Sidney Colowick has saddened his many
friends and colleagues. He will always be remembered for his significant
scientific contributions, his wise counsel, and his gentle humor.
JOHN J. LANGONE
HELEN VAN VUNAKIS
ixx
METHODS IN ENZYMOLOGY
EDITED YB
Sidney P. Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
iiixx
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick and Nathan O. Kaplan
VOLUME VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GINSBURG
etardyhobraC
VOLUME IX. Metabolism
Edited by WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited by RONALD W. AND MAYNARD ESTABROOK E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
VOLUME XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE NAMSSORG DNA KIVIE EVADLOM
VOLUME XIII. Citric Acid Cycle
Edited by J. M. LOWENSTEIN
VOLUME XIV. Lipids
Edited by J. M. LOWENSTEIN
VOLUME XV. Steroids and Terpenoids
Edited by RAYMOND B. NOTYALC
VOLUME XVI. Fast Reactions
Edited by KENNETH KUSTIN
VOLUME XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR AND CELIA WHITE TABOR
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