Immunochemical Techniques Part F: Conventional Antibodies, Fc Receptors, and Cytotoxicity

466 Pages·1983·11.7 MB·English

by  John J. Langone

Contributors to Volume 93
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
AERDNA C. ADAMS (15), Department of Mi- diatrics, University of Florida, Gaines-
crobiology, University of Notre Dame, ville, Florida 01623
Notre Dame, Indiana 65564 MURAT I. GHOSE (20), Department of Pa-
EUQINOM LEGAB (10), Laboratoire de Bio- thology, Dalhousie University, Halifax,
chimie des Antigdnes de Membrane, Nova Scotia B3H 4H7, Canada
931U Hopital Henri Mondor, 94010 Cre- HTEBAZXLE A. GRXMM (19), Department of
teil, France Health and Human Services, National In-
S. BEN-EFRAIM (3), Department of Human stitutes of Health, Bethesda, Maryland
Microbiology, Sackler School of Medi- 50202
cine, Tel-Aviv University, Tel-Aviv ,87996 HPESOJ HCIVOMIAH (9), Department of Hu-
Israel man Microbiology, Sackler School of
TIRAHCUS BHAKDI (24), The Institute of Medicine, Tel-Aviv University, TeI-Aviv
Medical Microbiology, University of ,87996 Israel
Giessen, D-6300 Giessen, Federal Repub- PAUL J. HIGGINS (5), Laboratory of Investi-
cil of Germany gative Cytology, Memorial Sloan-Ket-
A. HUNTLEY BLAIR (20), Department of tering Cancer Center, New York, New
Biochemistry, Dalhousie University, Hal- York 12001
ifax, Nova Scotia B3H 4H7, Canada TREBOR S. KERNEL (8), Department of Pa-
NIMAJNEB BONAVIDA (19), Department of thology, Cancer Research Laboratories,
Microbiology and Immunology, UCLA Queen's University, Kingston, Ontario
School of Medicine, University of -rofilaC K7L 3N6, Canada
nia, Los Angeles, California 42009 HILLEL S. KOREN (16), Department of Mi-
SAMOHT P. BRADLEY (19), Department of crobiology and Immunology, Duke Uni-
Microbiology and Immunology, UCLA versity Medical Center, Durham, North
School of Medicine, University of Cal- Carolina 01772
,ainrofi Los Angeles, California K. A. KCILORK (21), Department of Micro-
42009 biology, University of Texas Health Sci-
ENAITSIRHC CHARRIAUT (10), Laboratoire ences Center at San Antonio, San Anto-
de Biochimie des Antig~nes de Mem- nio, Texas 48287
brane, U139 Hopital Henri Mondor, YNOHTNA ,IKCYZCLUK JR. (12), Division of
01049 Creteil, France Allergy and Immunology, Washington
SELRAHC DELISl (7), Laboratory of Mathe- University School of Medicine, St. Louis,
matical Biology, National Institutes of Missouri 01136
Health, Bethesda, Maryland 50202 AJAMDAP N. KULKARNI (20), Department
ECURB E. TTO1LLE (8), Department of Pa- of Pathology, Dalhousie University, Hali-
thology, Cancer Research Laboratories, fax, Nova Scotia B3H 4H7, Canada
Queen's University, Kingston, Ontario JoE O. MINTA (23), Departments of Pathol-
K7L 3N6, Canada ogy, Medicine and the Rheumatic Dis-
DNOMYAR FRADE (10), Laboratoire de Bio- ease Unit, University of Toronto, -oT
chimie des Antig~nes de Membrane, ronto, Ontario M5S IA8, Canada
931/ Hopital Henri Mondor, 94010 Cre- NHOJ E. T. MOEN (17), Ministry of Housing,
teil, France Physical Planning and Environment, 0622
NAIRDA P. GEE (22, 23), Department of Pe- AK Leidschendam, ehT Netherlands
vii
viii CONTRIBUTORS TO VOL. 93
MARION MUHLY (24), The Institute of Medi- chemistry, The University of Texas Sys-
cal Microbiology, University of Giessen, tem Cancer Center, Houston, Texas
0036-D Giessen, Federal Republic of -reG 03077
many GEORGE R. SIBER (4), Division of Infectious
ELIZABETH O. PEPYS (11), Immunological Diseases, Sidney Farber Cancer Institute,
Medicine Unit, Department of Medicine, Boston, Massachusetts 51120
Royal Postgraduate Medical School, MORTON B. SIGEL (1), Whittier Institute for
Hammersmith Hospital, London 21W Diabetes and Endocrinology, La Jolla,
OHS, England California 73029
M. B. PEPYS (11), Immunological Medicine Y. N. SINHA (1), Whittier Institute for Dia-
Unit, Department of Medicine, Royal betes and Endocrinology, La Jolla, Cali-
Postgraduate Medical School, Hammer- fornia 73029
smith Hospital, London 21W OHS, Eng-
TSUNEO SUZUKI (14), Department of Micro-
land
biology, University of Kansas Medical
BERNARD J. RANSIL (4), Department of Health and Science Center, Kansas City,
Medicine, The Thorndike Laboratory and Kansas 30166
the Charles A. Dana Biomedical Re-
AMAR S. TUNG (2), Department of Immu-
search Institute, Harvard Medical
nology, Merck Sharp and Dohme Re-
School, Boston, Massachusetts 51220
search Laboratories, Rahway, New Jer-
E. H. RELYVELD (3), Department of Bacte- sey 56070
rial Vaccines, Institut Pasteur, 92380
J. W. UrIR (21), Department of Microbiol-
Garches, France
ogy, University of Texas Health Sciences
MARGIT ROTH (24), The Institute of Medical Center at Dallas, Dallas, Texas 53257
Microbiology, University of Giessen,
W. P. VANDERLAAN (1), Whittier Institute
0036-D Giessen, Federal Republic of -reG
for Diabetes and Endocrinology, La
many
Jolla, California 73029
SEYMOUR I. SCHLAGER (15, 18), Depart-
E. S. VITETTA (21), Department of Microbi-
ment of Microbiology, University of No-
tre Dame, Notre Dame, Indiana 65564 ology, University of Texas Health Sci-
ences Center at Dallas, Dallas, Texas
MAX SCHLAMOWITZ (13), Department of
53257
Biochemistry, ehT University of Texas
System Cancer Center, Houston, Texas SVEN O. WARNAAR (17), Department of Pa-
03077 thology, State University of Leiden, 2300
AL Leiden, ehT Netherlands
ALAIN B. SCHREIBER (9), Department of
Membrane Biology, Syntex Research, RIA NAV NIEUWMEGEN (6), Medical Fac-
Palo Alto, California 40349 ulty, Free University, 1007 MC Amster-
CARMEN A. SEPULVEDA (18), Department dam, ehT Netherlands
of Microbiology, University of Notre NIco NAV ROOUEN (6), Medical Faculty,
Dame, Notre Dame, Indiana 65564 Free University, 1007 MC Amsterdam,
ANITA R. SHAW (13), Department of -oiB ehT Netherlands
Preface
Antibodies clearly are extremely useful reagents in several research
areas, and many of the papers in the preceding volumes of Immunochemi-
cal Techniques have focused on their use in biochemical, immunological,
and clinical problems.
This volume includes papers dealing with the production of conven-
tional antibodies and vaccines, methods for the detection and isolation of
Fc receptors, and cytotoxicity assays (antibody- and cell-mediated). Im-
munochemical aspects of the classic and alternative complement systems
also are covered. They supplement several papers in Volume 08 that
covered isolation and biochemical properties of individual complement
components. Included also is a comprehensive treatment of the synthesis
and use of antibody-linked cytotoxic agents. Although there is a relatively
long history of such reagents prepared with conventional antibodies, the
principles also can be applied with monoclonal antibodies. These offer the
advantages of homogeneity and relatively strict and well-defined speci-
ficity.
Parts A-F of Immunochemical Techniques have dealt primarily with
the humoral immune system. Volumes emphasizing cellular aspects are in
the planning stage.
NHOJ J. ENOGNAL
NELEH NAV SIKANUV
xi
METHODS IN ENZYMOLOGY
EDITED BY
Sidney P. Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
ix
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick Nathan O. Kaplan
EMULOV VIII. Complex Carbohydrates
Edited yb HTEBAZILE F. DLEFUEN DNA ROTCIV GRUBSNIG
EMULOV IX. Carbohydrate Metabolism
Edited yb SILLLW A. DOOW
EMULOV X. Oxidation and Phosphorylation
Edited yb RONALD W. ESTABROOK AND MAYNARD E. PULLMAN
VOLUME XI. emyznE Structure
Edited by C. H. W. SRIH
EMULOV XII. Nucleic Acids (Parts A and )B
Edited by LAWRENCE GROSSMAN AND KIVlE MOLDAVE
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited yb J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited yb HTENNEK NITSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and )B
Edited by HERBERT TABOR AND CELIA WHITE TABOR
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DONALD B. MCCORMICK AND LEMUEL n. WRIGHT
VOLUME XlX. Proteolytic Enzymes
Edited yb GERTRUDE E. PERLMANN AND LASZLO LORAND
iiix
xiv METHODS IN YGOLOMYZNE
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part )B
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited yb ROTCIV GRUBSNIG
EMULOV XXlX. Nucleic Acids and Protein Synthesis (Part E)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVAOLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXlI. Biomembranes (Part B)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXlII. Cumulative Subject Index Volumes I-XXX
Edited yb AHTRAM G. SINNED DNA DRAWDE A. SINNED
METHODS IN ENZYMOLOGY XV
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited yb WILLIAM B. YBOKAJ DNA MEIR WILCHEK
EMULOV XXXV. Lipids (Part B)
Edited by JOHN M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited yb JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part )B
Edited yb W. A. WOOD
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited yb W. A. Wood
EMULOV XLIII. Antibiotics
Edited yb JOHN H. HASH
EMULOV XLIV. Immobilized Enzymes
Edited yb KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited yb LASZLO LORAND
EMULOV XLVI. Affinity Labeling
Edited yb WILLIAM B. YBOKAJ DNA MEIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited yb C. H. W. HIRS DNA SERGE N. TIMASHEFF
xvi
METHODS IN ENZYMOLOGY
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
EMULOV L. Complex Carbohydrates (Part C)
Edited by VICTOR GRUBSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. HOEFEE DNA MARY ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLEISCHER DNA LESTER REKCAP
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by SIDNEY FLEISCHER DNA LESTER REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by FLEISCHER SIDNEY DNA LESTER REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by MARLENE A. DELUCA
EMULOV LVIII. Cell Culture
Edited by WILLIAM I. YBOKAJ DNA ARI NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by KIVIE MOLDAVE DNA LAWRENCE NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVlE MOLDAVE DNA LAWRENCE NAMSSORG
EMULOV 61. Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA SERGE N. EFEHSAMIT
xvii
METHODS IN ENZYMOLOGY
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV .36 Enzyme Kinetics and Mechanism (Part A: Initial Rate and
Inhibitor Methods)
Edited yb LEINAD L. HCIRUP
EMULOV .46 Enzyme Kinetics and Mechanism (Part :B Isotopic Probes
and Complex Enzyme Systems)
Edited yb LEINAD L. HCIRUP
EMULOV .56 Nucleic Acids (Part I)
Edited yb ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited yb DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited yb DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV .86 Recombinant DNA
Edited yb YAR Wu
EMULOV .96 Photosynthesis and Nitrogen Fixation (Part )C
Edited yb YNOHTNA NAS ORTEIP
EMULOV .07 Immunochemical Techniques (Part A)
Edited yb NELEH NAV SIKANUV DNA NHOJ J. ENOGNAL
EMULOV .17 Lipids (Part C)
Edited yb NHOJ M. NIETSNEWOL
EMULOV .27 Lipids (Part D)
Edited by NHOJ M. NIETSNEWOL
EMULOV .37 Immunochemical Techniques (Part )B
Edited yb NHOJ J. ENOGNAL DNA NELEH NAV SIKANUV
EMULOV .47 Immunochemical Techniques (Part C)
Edited by NHOJ J. ENOGNAL DNA NELEH NAV SIKANUV
xviii SDOHTEM NI YGOLOMYZNE
EMULOV 75. Cumulative Subject Index Volumes XXXI, XXXII, and
XXXLIV-LX
Edited by EDWARD A. DENNIS DNA AHTRAM G. SINNED
EMULOV 76. Hemoglobins
Edited by ERALDO ANTONINI, LUIGI ROSSI-BERNARDI, AND EMILIA
CHIANCONE
EMULOV 77. Detoxication and Drug Metabolism
Edited by WILLIAM B. YBOKAJ
EMULOV 78. Interferons (Part A)
Edited by SIDNEY AKTSEP
EMULOV 79. Interferons (Part B)
Edited by SIDNEY AKTSEP
EMULOV 80. Proteolytic Enzymes (Part )C
Edited by LASZLO DNAROL
EMULOV 81. Biomembranes (Part H: Visual Pigments and Purple Mem-
branes, I)
Edited by LESTER REKCAP
EMULOV 82. Structural and Contractile Proteins (Part A: Extracellular
Matrix)
Edited by LEON W. CUNNINGHAM AND DIXIE W. FREDERIKSEN
EMULOV 83. Complex Carbohydrates (Part D)
Edited yb ROTCIV GRUBSNIG
EMULOV 84. Immunochemical Techniques (Part D: Selected Immunoas-
says)
Edited by JOHN J. LANGONE AND HELEN VAN VUNAKIS
EMULOV 85. Structural and Contractile Proteins (Part B: The Contractile
Apparatus and the Cytoskeleton)
Edited by DIXIE W. NESKIREDERF DNA LEON W. MAHGNINNUC
EMULOV 86. Prostaglandins and Arachidonate Metabolites
Edited by WILLIAM E. M. LANDS DNA WILLIAM L. HTIMS