Immunochemical Techniques Part E: Monoclonal Antibodies and General Immunoassay Methods

681 Pages·1983·18.99 MB·English

by  John J. Langone

Contributors to Volume 92
Article numbers are in parentheses following the names of contributors.
Affdiations listed are current.
N. LEIGH NOSREDNA (16), Molecular Anat- M. CHI~TIEN (23), Protein and Pituitary
omy Program, Division of Biological and Hormone Laboratory, Clinical Research
Medical Research, Argonne National Institute of Montreal, Montreal H2W
Laboratory, Argonne, Illinois 93046 IR7, Canada
GIULIA C. B. ASTALDI (5), lstituto di M. ANGELES SARERTNOC (22), School of
Genetica Biochimica ed Evolutionistica Applied Biology, Georgia Institute of
C.N.R. id Pavia, 27100 Pavia, Italy Technology, Atlanta, Georgia 23303
S1TARTS AVRAMEAS (38), D6partement LEGNA L. DE BLAS (4), Department of Neu-
d'lmmunologie, lnstitut Pasteur, 42757 robiology and Behavior, State University
Paris, Cedex ,51 France of New York at Stony Brook, Stony
Brook, New York 49711
MAILLIW F. BALE* (22), School of Applied
Biology, Georgia Institute of Technology, ENELRAM DELUCA (33), Department of
Atlanta, Georgia 23303 Chemistry, University of California, San
DRAHCIR B. TREKNAB (15, 21), Department Diego, La Jolla, California 39029
of Molecular Immunology, Roswell Park J. Y. DRALLIUOD (13), Institut National de
Memorial Institute, Buffalo, New York al eLBaS et de al Recherche Medicale
36241 (INSERM) U211, Unit( d' enseignement
TREBOR C. RETXAB (44), Department of En- et ed Recherche (UER) Medecine, 53044
docrinology, Royal Prince Alfred Hospi- Nantes, Cedex, France
tal, Sidney, New South Wales 2050, Aus- WEHTTAM J. DOYLE (34), Department of
ailart Chemistry, University of Cincinnati, Cin-
ELSA H. NIETSNEREB (2), Clinical Immunol- cinnati, Ohio 12254
ogy Section, Laboratory of Microbiology
V. B. ELINGS (36), Department of Physics,
and Immunology, National Institute of
University of California, Santa Barbara,
Dental Research, National Institutes of
California 60139
Health, Bethesda, Maryland 50202
J. SREGNIEF (28), Ames- Yissum Product Re-
J. SGGIRB (36), S YVA, Palo Alto, California
search and Development Laboratories,
30349
Jerusalem, Israel
HPESOJ P. BROWN (12), Program ni Tumor
RAMLEH FIEBACH (18), Department of Ex-
Immunology, Fred Hutchinson Cancer
perimental and Clinical Immunology,
Research Center, Seattle, Washington
Central Institute of Cancer Research,
4O189
Academy of Sciences of the G.D.R.,
TREBOR T. BUCKLER (32), Ames Research
DDR-III5 Berlin-Buch, German Demo-
and Development Laboratories, Miles
cratic Republic
Laboratories, Inc., Elkhart, Indiana
51564 PHILIP C. Fox (2), Clinical Immunology
Section, Laboratory of Microbiology and
DRAR~IG BUTT1N (14), Unit( de G~n(tique
Immunology, National Institute of Dental
Somatique, Bdtiment Metchnikoff, lnsti-
Research, National Institutes of Health,
tut Pasteur, 75724 Paris, Cedex ,51
Bethesda, Maryland 50202
France
LEAHCIM CAIS (25, 35), Department of G. M. FULLER (42), Department of Human
Chemistry, Technicon, Israel Institute of Biological Chemistry and Genetics, Divi-
Technology, Haifa 32000, Israel sion of Human Genetics, ehT University
*Deceased.
ix
X CONTRIBUTORS TO VOLUME 92
of Texas Medical Branch, Galveston, mie Analytique, Bdtiment Metchnikoff,
Texas 05577 Institut Pasteur, 75724 Paris, Cedex ,51
France
NAIRDA P. GEE (31), Department of Pediat-
rics, University of Florida, Gainesville, YRNEH S. NALPAK (i), Department of Radi-
ology, Cancer Biology Research Labora-
Florida 01623
tory, Stanford University School of Medi-
V. GHETIE (40), Department of Immunol-
cine, Stanford, California 50349
ogy, Victor Babe¢ Institute, 10267 Bucha-
UWE NETSRAK (18), Department of Experi-
rest, Romania
mental and Clinical Immunology, Central
JEAN-LUC GUI~SDON (38), Ddpartement Institute of Cancer Research, Academy of
d'Immunologie, lnstitut Pasteur, 42757
Sciences of the G.D.R., DDR-I I15
Paris, Cedex ,51 France Berlin-Buch, German Democratic Re-
H. BRIAN HALSALL (34), Department of public
Chemistry, University of Cincinnati, Cin- ASUFENAK KATO (26), Department of -DiB
cinnati, Ohio 12254 chemistry, Institute for Developmental
BRUNO HANSEN (24), Hagedorn Research Research, Aichi Prefectural Colony, Ka-
Laboratory, 2820 Gentofte, Denmark sugai, Aichi ,30-084 Japan
P. H.~RING (20), Research Department, F. NHOJ J. ENOGNAL (31), Department of Med-
Hoffmann-La Roche and Co., Ltd., CH- icine, Baylor College of Medicine, Hous-
0024 Basel, Switzerland ton, Texas 03077
WILLIAM R. HEINEMAN (34), Department ERREIP LEGRAIN (14), Unitd de Gdndtique
of Chemistry, University of Cincinnati, Somatique, Bdtiment Metehnikoff, lnsti-
Cincinnati, Ohio 12254 tat Pasteur, 75724 Paris, Cedex ,51
France
DREGEGNI MORTSLLEH (12), Program ni -uT
• EK~ KRAMNREL (24), Hagedorn Research
mor Immunology, Fred Hutchinson Can-
Laboratory, 2820 Gentofte, Denmark
cer Research Center, and Department of
Microbiology~Immunology, University of ENNASUS LINDE (24), Hagedorn Research
Washington, Seattle, Washington 40189 Laboratory, 2820 Gentofte, Denmark
KARL ERIK MORTSLLEH (12), Program ni Bo NOSSAITTAM (39), Pure and Applied Bio-
Tumor Immunology, Fred Hutchinson chemistry, Chemical Center, University
Cancer Research Center, and Depart- of Lund, S-220 07 Lund, Sweden
ment of Pathology, University of Wash- EGROEG L. MAYERS (21), Department of
ington, Seattle, Washington 40189 Molecular Immunology, Roswell Park
NEVETS H. NNAMRREH (8), Department of Memorial Institute, Buffalo, New York
Pathology, Harvard Medical School, 36241
Boston, Massachusetts 51120 KRAP K. ORREFAZZAM (21), Department of
T. HOFFMAN (13), Division of Biochemistry Molecular Immunology, Roswell Park
and Biophysics, Office of Biologics, FDA, Memorial Institute, Buffalo, New York
National Center for Drugs and Biologics, 36241
Bethesda, Maryland 50202 DLANOR McKAY (10), Cold Spring Harbor
D. INBAR (28), Ames-Yissum Product Re- Laboratory, Cold Spring Harbor, New
search and Development Laboratories, York 42711
Jerusalem, Israel WEHTTAM F. REHCSEM (8), Department of
YI-HER Jou (21), Department of Molecular Pathology, Harvard Medical School,
Immunology, Roswell Park Memorial In- Boston, Massachusetts 51120
stitute, Buffalo, New York 36241 DRAI-IKRUB MlCrIEEL (18), Department of
EUQINIMOD JUY (14), Unitd d'Immunochi- Experimental and Clinical Immunology,
CONTRIBUTORS TO VOLUME 92 xi
Central Institute of Cancer Research, ESOJ M. ATLAREP (29, 30), Instituto de Mi-
Academy of Sciences of the G.D.R., crobiologia, Universidade Federal do Rio
DDR-II15 Berlin-Buch, German Demo- de Janeiro, Rio de Janeiro 21491, Brasil
cratic Republic A. J. PICK (28), Ames-Yissum Product Re-
V. MIGGIANO (3, 20), Research Depart- search and Development Laboratories,
ment, F. Hoffmann-La Roche and Co., Jerusalem, Israel
Ltd., CH-4200 Basel, Switzerland M. A. OTALOZZIP (17), Department of -inilC
I. URAROM (40), Department of Immunol- cal Chemistry, Faculty of Biochemistry,
ogy, Victor Babe~ Institute, 10267 Bucha- University of Buenos Aires, Buenos Aires
rest, Romania ,3111 Argentina
DIVAD L. MoRius (32), Ames Research and DNARAKAM V. IHKRAPANTAR (4), Depart-
Development Laboratories, Miles Labo- ment of Mathematics and Statistics,
ratories, Inc., Elkhart, Indiana 51564 Wright State University, Dayton, Ohio
SEMAJ E. MOSIMANN (4), Laboratory of 53454
Statistical and Mathematical Methodol- D. G. RITCHIE (42), Department of Human
ogy, Division of Computer Research and Biological Chemistry and Genetics, Divi-
Technology, National Institutes of sion of Cell Biology, ehT University of
Health, Bethesda, Maryland 50202 Texas Medical Branch, Galveston, Texas
ROLF MOLLER (43), Tumor Virology Labo- 05577
ratory, Salk Institute, San Diego, Califor- SAMOHT J. SREGOR (19), Department of Mi-
ain 83129 crobiology and lmmunology, Temple Uni-
RETEP J. MUNSON (41), Endocrinology and versity School of Medicine, Philadelphia,
Reproduction Research Branch, National Pennsylvania 40191
Institutes of Child Health and Human De- N. G. SEIDAH (23), Protein and Pituitary
velopment, National Institutes of Health, Hormone Laboratory, Clinical Research
Bethesda, Maryland 50202 Institute of Montreal, Montreal H2W
J. M. NOSREKCIN (42), Laboratory of Mo- 1R7, Canada
lecular Genetics, National Institutes of ETHAN M. HCAVEHS (7), Laboratory of Im-
Health, Bethesda, Maryland 50202 munology, National Institute of Allergy
J. C. NICOLAS (27), INSERM Unitd ,85 and Infectious Diseases, National Insti-
Montpellier ,00143 France tutes of Health, Bethesda, Maryland
D. F. NlCOLI (36), Department of Physics, 50202
University of California, Santa Barbara, A. YAR SIMONS (29), Laboratory Training
California 60139 and Consultation Division, Centers for
NEELHTAK O'DAY (19), School of Veteri- Disease Control, Atlanta, Georgia 33303
nary Medicine, Oregon State University, NEBUER P. NAINAGARIS (2), Clinical Immu-
Corvallis, Oregon 13379 nology Section, Laboratory of Microbiol-
TRANNEL NOSSLO (1), Department of Medi- ogy and Immunology, National Institute
cine A, State University Hospital, Copen- of Dental Research, National Institutes of
hagen 2100, Denmark Health, Bethesda, Maryland 50202
PETER MAHRAP (9), Department of Struc- GNIVRI L. SPAR (22), Department of Radia-
tural Biology, Stanford University School tion Biology and Biophysics, University of
of Medicine, Stanford, California 50349 Rochester, Rochester, New York 72641
YRRET W. NOSRAEP (16), Department of YHTOMIT A. REGNIRPS (11), Laboratory of
Biochemistry and Microbiology, Univer- Membrane Immunochemistry, Sidney
sity of Victoria, Victoria, British Colum- Farber Cancer Institute, Boston, Massa-
aib V8W 2Y2, Canada chusetts 51120
xii
CONTRIBUTORS TO VOLUME 92
TH. STAEHELIN (3, 20), Research Depart- AMAR S. TUNG (6), Department of Immu-
ment, F. Hoffmann-La Roche and Co., nology, Merck Sharp and Dohme Re-
Ltd., CH-4200 Basel, Switzerland search Laboratories, Rahway, New Jer-
C. ,ATS ILH (3, 20), Research Department, F. sey 56070
Hoffmann-La Roche and Co., Ltd., CH- NORAA P. TURKEWlTZ (8), Department of
0024 Basel, Switzerland Pathology, Harvard Medical School,
NYRHTAK C. PUCLLATS (8), Department of Boston, Massachusetts 51120
Pathology, Harvard Medical School, O. B. RENGAW (28), Becton Dickinson Re-
Boston, Massachusetts 51120 search Center, Research Triangle Park,
J. REKCOTS (20), Research Department, F. North Carolina 90772
Hoffmann-La Roche and Co., Ltd., CH- NOJ WANNLUND (33), Analytical Lumines-
0024 Basel, Switzerland cence Laboratory, San Diego, California
NEELHTAC P. NAVILLUS (8), Department of 12129
Pathology, Harvard Medical School, HTENNEK R. REYEMHEW (34), Department
Boston, Massachusetts 51120 of Chemistry, University of Cincinnati,
B. SCAKAT (20), Research Department, F. Cincinnati, Ohio 12254
Hoffmann-La Roche and Co,, Ltd., CH- O. WEISS (28), Ames-Yissum Product Re-
0024 Basel, Switzerland search and Development Laboratories,
Y. TAMIR (28), Ames-Yissum Product Re- Jerusalem, Israel
search and Development Laboratories, TT1RB C. NOSLIW (30), 2238 Lyle Road, Col-
Jerusalem, Israel lege Park, Georgia 30337
B. ENNAUORET (27), INSERM Unitd ,85 LEON WOFSY (37), Department of Microbi-
Montpellier 34100, France ology and Immunology, University of
ROTCIV C. W. TSANG (29, 30), Helminthic California, Berkeley, California 02749
Diseases Branch, Division of Parasitic NEHPETS E. ZWEIG (7), Department of
Diseases, Centers for Disease Control, Pharmacology, Baylor University College
Atlanta, Georgia 33303 of Medicine, Houston, Texas 03077
Preface
Several papers in this volume deal with advances in hybridoma tech-
nology and complement the chapter by Galfr5 and Milstein that appeared
in Volume 73 of this series. Methods to enhance the efficiency of produc-
ing desired fusion products, to simplify the basic procedures involved,
and to assess the specificity and other properties of monoclonal antibod-
ies are discussed. Representative examples illustrate how a battery of
monoclonal antibodies with different specificities can be used to study
various immunochemical and biochemical problems. Other chapters
Cover additional procedures that can be used to label antigens and anti-
bodies, separate antigen-antibody complexes, and process data. Some
papers classified under one heading or contained in another volume of the
series include methods that can be applied in other specific areas. For
example, cytotoxicity tests described in Volume 93 may be used to screen
supernatant fluids in hybridoma experiments if cytotoxic activity is a
desirable property of the monoclonal antibody.
The contents of these volumes continue to reflect our intention of
presenting useful methods as they are developed. Thus, several section
headings may be similar to those in other Immunochemical Techniques
volumes. We believe that such a system makes it easier to follow recent
advances. It can be particularly advantageous to investigators whose ma-
jor interests may lie outside the realm of immunology but who realize that
answers to their scientific problems can often be obtained by the judicious
use of innovative immunochemical techniques.
Again we thank the contributors whose consistently fine efforts make
these volumes possible; Carla Langone for managing the correspondence;
and Nathan Kaplan, Sidney Colowick, and our colleagues at Academic
Press for their continued enthusiastic support and invaluable assistance.
JOHN J. LANGONE
HELEN VAN VUNAKIS
iiix
METHODS IN ENZYMOLOGY
EDITED BY
Sidney P. Colowick and Nathan O. Kaplan
VANDERB1LT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
Ill. Preparation and Assay of Substrates
IV, Special Techniques for the Enzymologist
V, Preparation and Assay of Enzymes
VI, Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Techniques Special
VII. Subject Cumulative Index
VX
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick Nathan O. Kaplan
EMULOV VIII. Complex Carbohydrates
Edited yb ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
EMULOV IX. etardyhobraC Metabolism
Edited yb WILLIS A. WOOD
EMULOV X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. PULLMAN
EMULOV XI. Enzyme Structure
Edited yb C" H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited yb LAWRENCE NAMSSORG DNA KIVIE EVADLOM
EMULOV XIII. Citric Acid Cycle
Edited yb J. M. LOWENSTEIN
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited yb DNOMYAR B. NOTYALC
EMULOV XVI. Fast Reactions
Edited yb KENNETH KUSTIN
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited yb HERBERT TABOR DNA CELIA WHITE ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DONALD B. MCCORMICK DNA LEMUEL D. WRIGHT
EMULOV XXX. cityloetorP semyznE
Edited yb GERTRUDE E. PERLMANN DNA LASZLO LORAND
iivx
xviii METHODS IN ENZYMOLOGY
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. EFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. EEEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by AH'lRAM G. SINNED DNA DRAWDE A. SINNED
METHODS IN ENZYMOLOGY xix
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. YBOKAJ DNA MEIR WILCHEK
VOLUME XXXV. Lipids (Part B)
Edited yb JOHN M. LOWENSTEIN
VOLUME XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
VOLUME XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited yb BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited yb JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited yb JOEL G. HARDMAN DNA BERT W. O'MALLEY
EMULOV XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited yb W. A. WOOD
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited yb W. A. WOOD
EMULOV XLIII. Antibiotics
Edited yb JOHN H. HASH
VOLUME XLIV. Immobilized Enzymes
Edited yb KLAUS HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
Edited yb LORAND LASZLO
EMULOV XLVI. Affinity Labeling
Edited by WILLIAM B. YBOKAJ DNA MEIR WILCHEK
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS DNA SERGE N. TIMASHEFF
XX METHODS IN ENZYMOLOGY
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited yb C. H. W. HIRS DNA EGRES N. EEEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited yb ROTCIV GRUnSNIG
EMULOV LI. Purine and Pyrimidine Nucleotide Metabolism
Edited by AICIRTAP A. HOFFEE DNA YRAM ELLEN SENOJ
EMULOV LII. Biomembranes (Part C: Biological Oxidations)
Edited by REHCSIELF YENDIS DNA LESTER REKCAP
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited yb REHCSIELF YENDIS DNA LESTER REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by REHCSIELF YENDIS DNA LESTER REKCAP
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by REHCSIELF YENDIS DNA LESTER REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by REHCSIELF YENDIS DNA LESTER REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. DELUCA
EMULOV LVII1. Cell Culture
Edited yb WILLIAM B. YBOKAJ DNA ARI H. NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. W. HIRS DNA EGRES N. EFEHSAMIT