Table Of ContentIMMUNOASSAYS IN
AGRICULTURAL
BIOTECHNOLOGY
IMMUNOASSAYS IN
AGRICULTURAL
BIOTECHNOLOGY
Edited by
GUOMIN SHAN
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10 9 8 7 6 5 4 3 2 1
CONTENTS
FOREWORD VII
PREFACE IX
CONTRIBUTORS XI
CHAPTER1 INTRODUCTION 1
GuominShan
CHAPTER2 PRINCIPLESOFIMMUNOASSAYS 5
ClaudiaSheedyandKerrmY.Yau
CHAPTER3 ANTIBODYENGINEERINGINAGRICULTURALBIOTECHNOLOGY 23
PatrickDoyle,MehdiArbabi-Ghahroudi,ClaudiaSheedy,
KerrmY.Yau,and J.ChristopherHall
CHAPTER4 MICROTITERPLATEELISA 59
MichaelC.Brown
CHAPTER5 LATERALFLOWDEVICES 91
MuraliBandla,RickThompson,andGuominShan
CHAPTER6 IMMUNOASSAYMETHODVALIDATION 115
JeanSchmidtandClaraAlarcon
CHAPTER7 REFERENCEMATERIALSANDCONSIDERATIONS 139
TandaceA.ScholdbergandG.RonaldJenkins
CHAPTER8 AUTOMATIONOFIMMUNOASSAYS 175
MicheleYarnall
CHAPTER9 DATAINTERPRETATIONANDSOURCESOFERROR 189
RodA.HermanandGuominShan
v
vi CONTENTS
CHAPTER10 IMMUNOASSAYAPPLICATIONSINTRAITDISCOVERY,
PRODUCTDEVELOPMENT,ANDREGISTRATION 219
BerylPacker,AndreSilvanovich,andG.DavidGrothaus
CHAPTER11 IMMUNOASSAYAPPLICATIONSINGRAINPRODUCTS
ANDFOODPROCESSING 241
GinaM.ClapperandLuluKurman
CHAPTER12 IMMUNOASSAYAPPLICATIONSINSOILMONITORING 257
GuominShan
CHAPTER13 IMMUNOASSAYAPPLICATIONSINPLANT-BASEDBIOPHARMA 271
ThomasPattersonandGregoryGilles
CHAPTER14 IMMUNOASSAYSINVETERINARYPLANT-MADEVACCINES 289
GiorgioDeGuzman,RobertP.Shepherd,andAmandaM.Walmsley
CHAPTER15 IMMUNOASSAYASAGMDETECTIONMETHODIN
INTERNATIONALTRADE 309
RayShillitoandThomasCurrier
CHAPTER16 FUTUREPERSPECTIVESANDCHALLENGES 325
ZiLucyLiu,Ai-GuoGao,LeslieA.Harrison,KerrmY.Yau,JohnLawry,
andGuominShan
INDEX 341
FOREWORD
Thepowerfulanalyticaltechniqueofimmunoassayhasexperiencedever-expanding
use in a variety of applications and settings since the pioneering work of Yallow
and Berson a half century ago. The application of immunoassays to agricultural
biotechnologyisexpandingandthuscreatesagreatneedforawidevarietyofusersto
understand and implementimmunoassays andrelated technologies.
Thisbookdescribestheapplicationofimmunoassaytechnologyinagricultural
biotechnologyproductdiscovery,researchanddevelopment,manufacturing,quality
control,andnationalandinternationalregulatorycompliance.Oneuniquefeatureof
thisbookisitscontributors,whoareexperiencedpractitionersfrommajoragricultural
biotechcompanies,immunoassayreagentmanufacturers,academicresearchinstitu-
tions,andgovernment agencies.Coveringawidevarietyoftopics, the bookoffers
practical guidance for assay/kit development, assay validation, data analysis and
implementationaswellasdiscussionsofnewtechnologiesandperspectives.Itisan
annotatedwindowintopreviousliteratureaswellasatextprovidingboththeoryand
practicalinstructions.Thisbookwillfindwidespreadutilityamongallthoseinvolved
in thisexciting newfield.
BRUCE HAMMOCK
Distinguished Professor ofEntomology &
CancerResearch Center
University ofCalifornia, Davis
vii
Description:A very broad range of professionals are using immunoassay technology daily to analyze genetically engineered (GE) crops and related areas, and many of these professionals are completely new to this technology. There is a great need for users to have a book containing technical and practical guidance
