Table Of ContentMETHODS IN ENZYMOLOGY
Editors-in-Chief
JOHN N. ABELSON AND MELVIN I. SIMON
Division of Biology
California Institute of Technology
Pasadena, California
Founding Editors
SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
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First edition 2012
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CONTRIBUTORS
Volker Andresen
Rudolf-Virchow Center for Experimental Biomedicine and Department of
Dermatology, Venerology and Allergology, University of Wu¨rzburg, Wu¨rzburg,
Germany; and LaVision BioTec GmbH, Bielefeld, Germany
Michael C. Ashby
MRC Centre for Synaptic Plasticity, School of Biochemistry, Medical Sciences
Building and School of Physiology and Pharmacology, University of Bristol,
Bristol, United Kingdom
Gert-Jan Bakker
Microscopical Imaging of the Cell, Department of Cell Biology, Nijmegen Center
for Molecular Life Science, Radboud University Nijmegen Medical Centre,
Nijmegen, The Netherlands
Graeme Ball
Department of Biochemistry, The University of Oxford, Oxford, United
Kingdom
Marin Barisic
Chromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular
e Celular, Universidade do Porto, Porto, Portugal
Kiah Barton
Department of Molecular and Cellular Biology, Laboratory of Plant Development
and Interactions, University of Guelph, Guelph, Canada
Andre´ E.P. Bastos
Centro de Qu´ımica e Bioqu´ımica, Faculdade de Cieˆncias da Universidade de
Lisboa, Lisboa, Portugal
Galina V. Beznoussenko
Istituto FIRC di Oncologia Molecolare, Milan, Italy
Paola Borri
School of Biosciences, Cardiff University, Cardiff, United Kingdom
Stanley W. Botchway
Research Complex at Harwell, Central Laser Facility, STFC, Rutherford Appleton
Laboratory, Harwell-Oxford, Didcot, Oxford, Oxfordshire, United Kingdom
xi
xii Contributors
Jonathan M. Crane
Departments of Medicine and Physiology, University of California, San Francisco,
San Francisco, California, USA
Michael W. Davidson
National High Magnetic Field Laboratory and Department of Biological Science,
The Florida State University, Tallahassee, Florida, USA
Ilan Davis
Department of Biochemistry, The University of Oxford, Oxford, United
Kingdom
Richard N. Day
Department of Cellular and Integrative Physiology, Indiana University School of
Medicine, Indianapolis, Indiana, USA
Rodrigo F.M. de Almeida
Centro de Qu´ımica e Bioqu´ımica, Faculdade de Cieˆncias da Universidade de
Lisboa, Lisboa, Portugal
Oleh Dzyubachyk
Biomedical Imaging Group Rotterdam, Departments of Medical Informatics and
Radiology, Erasmus MC—University Medical Center Rotterdam, Rotterdam,
The Netherlands
Ning Fang
Ames Laboratory, U.S. Department of Energy and Department of Chemistry, Iowa
State University, Ames, Iowa, USA
Peter Friedl
Microscopical Imaging of the Cell, Department of Cell Biology, Nijmegen Center
for Molecular Life Science, Radboud University Nijmegen Medical Centre,
Nijmegen, The Netherlands; Rudolf-Virchow Center for Experimental Biomedi-
cine and Department of Dermatology, Venerology and Allergology, University of
Wu¨rzburg, Wu¨rzburg, Germany
Katharina Gaus
Centre for Vascular Research, University of New South Wales, Sydney, Australia
Yves Goldberg
MRC Centre for Synaptic Plasticity, School of Biochemistry, Medical Sciences
Building, University of Bristol, Bristol, United Kingdom
Inmaculada M. Gonza´lez-Gonza´lez
MRC Centre for Synaptic Plasticity, School of Biochemistry, Medical Sciences
Building, University of Bristol, Bristol, United Kingdom
Contributors xiii
Sarah Griffiths
Department of Molecular and Cellular Biology, Laboratory of Plant Development
and Interactions, University of Guelph, Guelph, Canada
Nicholas A. Hamilton
Division of Genomics Computational Biology, Institute for Molecular Bioscience,
The University of Queensland, St. Lucia, Brisbane, Queensland, Australia
Russell S. Hamilton
Department of Biochemistry, The University of Oxford, Oxford, United
Kingdom
Nicole M. Hays
Department of Cellular and Integrative Physiology, Indiana University School of
Medicine, Indianapolis, Indiana, USA
Jeremy M. Henley
MRC Centre for Synaptic Plasticity, School of Biochemistry, Medical Sciences
Building, University of Bristol, Bristol, United Kingdom
Katie J. Herbst-Robinson
Department of Pharmacology and Molecular Sciences, The Johns Hopkins
University School of Medicine, Baltimore, Maryland, USA
Robert M. Hoffman
AntiCancer, Inc. and Department of Surgery, University of California San Diego,
San Diego, California, USA
Fre´de´ric Jaskolski
MRC Centre for Synaptic Plasticity, School of Biochemistry, Medical Sciences
Building, University of Bristol, Bristol, United Kingdom
Satoshi Kametaka
Department of Anatomy and Histology, Fukushima Medical University School of
Medicine, Fukushima, Japan
Kenichi Kawano
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto,
Japan
Yong-Eun Koo Lee
Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA
Raoul Kopelman
Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA
Wolfgang Langbein
School of Physics and Astronomy, Cardiff University, Cardiff, United Kingdom
Astrid Magenau
Centre for Vascular Research, University of New South Wales, Sydney, Australia
xiv Contributors
Helder Maiato
Chromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular
e Celular and Department of Experimental Biology, Faculdade de Medicina,
Universidade do Porto, Porto, Portugal
Jaideep Mathur
Department of Molecular and Cellular Biology, Laboratory of Plant Development
and Interactions, University of Guelph, Guelph, Canada
Katsumi Matsuzaki
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto,
Japan
Erik Meijering
Biomedical Imaging Group Rotterdam, Departments of Medical Informatics and
Radiology, Erasmus MC—University Medical Center Rotterdam, Rotterdam,
The Netherlands
Alexander A. Mironov
Istituto FIRC di Oncologia Molecolare, Milan, Italy
Lyndsay Murrow
Department of Pathology, University of California, San Francisco, San Francisco,
California, USA
Michaela Nebel
Analytische Chemie, Elektroanalytik & Sensorik, Ruhr-Universita¨t Bochum,
Bochum, Germany
Peter O’Neill
MRC/CRUK Gray Institute for Radiation Oncology and Biology, University of
Oxford, Oxford, Oxfordshire, United Kingdom
Kaoru Omae
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto,
Japan
Dylan M. Owen
Centre for Vascular Research, University of New South Wales, Sydney, Australia
Anthony W. Parker
Research Complex at Harwell, Central Laser Facility, STFC, Rutherford Appleton
Laboratory, Harwell-Oxford, Didcot, Oxford, Oxfordshire, United Kingdom
Richard M. Parton
Department of Biochemistry, The University of Oxford, Oxford, United
Kingdom
Contributors xv
Hayley Pemble
Department of Cell & Tissue Biology, University of California, San Francisco,
San Francisco, California, USA
Anto´nio J. Pereira
Chromosome Instability & Dynamics Laboratory, Instituto de Biologia Molecular
e Celular, Universidade do Porto, Porto, Portugal
Ammasi Periasamy
W.M. Keck Center for Cellular Imaging; Departments of Biology and Biomedical
Engineering, University of Virginia, Charlottesville, Virginia, USA
Iestyn Pope
School of Biosciences, Cardiff University, Cardiff, United Kingdom
Pamela Reynolds
MRC/CRUK Gray Institute for Radiation Oncology and Biology, University of
Oxford, Oxford, Oxfordshire, United Kingdom
Andrea Rossi
Departments of Medicine and Physiology, University of California, San Francisco,
San Francisco, California, USA
Martin H. Schattat
Department of Molecular and Cellular Biology, Laboratory of Plant Development
and Interactions, University of Guelph, Guelph, Canada
Wolfgang Schuhmann
Analytische Chemie, Elektroanalytik & Sensorik, Ruhr-Universita¨t Bochum,
Bochum, Germany
Albert Schulte
Biochemistry-Electrochemistry Research Unit, Schools of Chemistry and Bio-
chemistry, Institute of Science, Suranaree University of Technology, Nakhon
Ratchasima, Thailand
Silvia Scolari
Centro de Qu´ımica e Bioqu´ımica, Faculdade de Cieˆncias da Universidade de
Lisboa, Lisboa, Portugal
Ihor Smal
Biomedical Imaging Group Rotterdam, Departments of Medical Informatics and
Radiology, Erasmus MC—University Medical Center Rotterdam, Rotterdam,
The Netherlands
Martin Sto¨ckl
Nanobiophysics, MESAþ Institute for Nanotechnology, University of Twente,
Enschede, The Netherlands
xvi Contributors
Samantha Stehbens
Department of Cell & Tissue Biology, University of California, San Francisco,
San Francisco, California, USA
Yuansheng Sun
W.M. Keck Center for Cellular Imaging; Departments of Biology and Biomedical
Engineering, University of Virginia, Charlottesville, Virginia, USA
A.S. Verkman
Departments of Medicine and Physiology, University of California, San Francisco,
San Francisco, California, USA
Satoshi Waguri
Department of Anatomy and Histology, Fukushima Medical University School of
Medicine, Fukushima, Japan
Gufeng Wang
Department of Chemistry, North Carolina State University, Raleigh, North
Carolina, USA
Peter Watson
School of Biosciences, Cardiff University, Cardiff, United Kingdom
David Williamson
Centre for Vascular Research, University of New South Wales, Sydney, Australia
Torsten Wittmann
Department of Cell & Tissue Biology, University of California, San Francisco,
San Francisco, California, USA
Yoshiaki Yano
Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto,
Japan
Jin Zhang
Department of Pharmacology and Molecular Sciences and The Solomon
H. Snyder Departments of Neuroscience and Oncology, The Johns Hopkins
University School of Medicine, Baltimore, Maryland, USA
Xin Zhou
Department of Pharmacology and Molecular Sciences, The Johns Hopkins
University School of Medicine, Baltimore, Maryland, USA
PREFACE
Going back to the dawn of light microscopy, imaging techniques have
provided the opportunity for developing models of cellular function.
Over the past 40 years, the availability of technology for high-resolution
imaging and for evaluation of those images in live cells has extended our
reach and, accordingly, our ability to understand cell function. Given the
large number of choices in equipment and approaches, sorting out the best
approach can be challenging, even to seasoned investigators.
The present volumes provide descriptions of methods used to image
living cells, with particular reference to the technical approaches and
reagents needed and approaches to selecting the best techniques. The
authors explain how these methods are able to provide important biological
insights in normal and pathological cells.
Authors were selected based on research contributions in the area about
which they have written and based on their ability to describe their meth-
odological contribution in a clear and reproducible way. They have been
encouraged to make use of graphics, comparisons to other methods, and to
provide tricks and approaches not revealed in prior publications that make it
possible to adapt methods to other systems.
The editor wants to express appreciation to the contributors for
providing their contributions in a timely fashion, to the senior editors for
guidance, and to the staff at Academic Press for helpful input.
P. MICHAEL CONN
xvii
METHODS IN ENZYMOLOGY
VOLUME I. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME II. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME III. Preparation and Assay of Substrates
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME V. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME VII. Cumulative Subject Index
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD AND VICTOR GINSBURG
VOLUME IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited by RONALD W. ESTABROOK AND MAYNARD E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
VOLUME XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
VOLUME XIII. Citric Acid Cycle
Edited by J. M. LOWENSTEIN
VOLUME XIV. Lipids
Edited by J. M. LOWENSTEIN
VOLUME XV. Steroids and Terpenoids
Edited by RAYMOND B. CLAYTON
xix
