Table Of ContentMETHODS IN ENZYMOLOGY
Editors-in-Chief
JOHN N. ABELSON and MELVIN I. SIMON
Division of Biology
California Institute of Technology
Pasadena, California
Founding Editors
SIDNEY P. COLOWICK and NATHAN O. KAPLAN
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CONTRIBUTORS
FernandoAntunes
DepartamentodeQu´ımicaeBioqu´ımicaandCentrodeQu´ımicaeBioqu´ımica,Faculdade
deCieˆncias,UniversidadedeLisboa,Lisboa,Portugal
AnaG.Barata
DivisionofRedoxRegulation,GermanCancerResearchCenter(DKFZ),DKFZ-ZMBH
Alliance,Heidelberg,Germany
VsevolodV.Belousov
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,andNizhny
NovgorodStateMedicalAcademy,NizhnyNovgorod,Russia
DmitryS.Bilan
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,andNizhny
NovgorodStateMedicalAcademy,NizhnyNovgorod,Russia
MartinD.Brand
TheBuckInstituteforResearchonAging,Novato,California,USA
EnriqueCadenas
Pharmacology&PharmaceuticalSciences,SchoolofPharmacy,UniversityofSouthern
California,LosAngeles,California,USA
ChristopherJ.Chang
DepartmentofChemistry;DepartmentofMolecularandCellBiology,Universityof
California,Berkeley,California,andHowardHughesMedicalInstitute,ChevyChase,
Maryland,USA
Lu´ısaCyrne
DepartamentodeQu´ımicaeBioqu´ımicaandCentrodeQu´ımicaeBioqu´ımica,Faculdade
deCieˆncias,UniversidadedeLisboa,Lisboa,Portugal
BryanC.Dickinson
DepartmentofChemistryandChemicalBiology,HarvardUniversity,Cambridge,
Massachusetts,USA
TobiasP.Dick
DivisionofRedoxRegulation,GermanCancerResearchCenter(DKFZ),DKFZ-ZMBH
Alliance,Heidelberg,Germany
JamesEnomoto
DepartmentofBiomedicalEngineering,UniversityofCalifornia,Davis,California,USA
ArkadyF.Fradkov
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,Russia
DouglasGanini
LaboratoryofToxicologyandPharmacology,NationalInstituteofEnvironmentalHealth
Sciences,NIH,ResearchTrianglePark,NorthCarolina,USA
xi
xii Contributors
MarcoGiorgio
DepartmentofExperimentalOncology,EuropeanInstituteofOncology,Milan,Italy
RenataL.S.Goncalves
TheBuckInstituteforResearchonAging,Novato,California,USA
LongweiHe
StateKeyLaboratoryofChemo/BiosensingandChemometrics,CollegeofChemistryand
ChemicalEngineering,HunanUniversity,Changsha,PRChina
MartinHey-Mogensen
TheBuckInstituteforResearchonAging,Novato,California,USA,andCenterforHealthy
Aging,DepartmentofBiomedicalSciences,CopenhagenUniversity,Copenhagen,Denmark
EtsuroIto
KagawaSchoolofPharmaceuticalSciences,TokushimaBunriUniversity,Sanuki,Japan
DeanP.Jones
DivisionofPulmonary,AllergyandCriticalCareMedicine,ClinicalBiomarkers
Laboratory,DepartmentofMedicine,EmoryUniversitySchoolofMedicine,Atlanta,
Georgia,USA
JoyJoseph
DepartmentofBiophysicsandFreeRadicalResearchCenter,MedicalCollegeofWisconsin,
Milwaukee,Wisconsin,USA
BalaramanKalyanaraman
DepartmentofBiophysicsandFreeRadicalResearchCenter,MedicalCollegeofWisconsin,
Milwaukee,Wisconsin,USA
RandalJ.Kaufman
CenterforNeuroscience,Aging,andStemCellResearch,Sanford-BurnhamMedical
ResearchInstitute,LaJolla,California,USA
MelissaL.Kemp
TheWallaceH.CoulterDepartmentofBiomedicalEngineering,GeorgiaInstituteof
TechnologyandEmoryUniversity,Atlanta,Georgia,USA
ArielKniss
TheWallaceH.CoulterDepartmentofBiomedicalEngineering,GeorgiaInstituteof
TechnologyandEmoryUniversity,Atlanta,Georgia,USA
VamsiK.Kodali
CenterforNeuroscience,Aging,andStemCellResearch,Sanford-BurnhamMedical
ResearchInstitute,LaJolla,California,USA
HiromiKodama
KagawaSchoolofPharmaceuticalSciences,TokushimaBunriUniversity,Sanuki,Japan
GuyLandau
CenterforNeuroscience,Aging,andStemCellResearch,Sanford-BurnhamMedical
ResearchInstitute,LaJolla,California,USA
DavidLiebl
InstituteforMolecularBioscience,TheUniversityofQueensland,St.Lucia,Queensland,
Australia
Contributors xiii
VivianS.Lin
DepartmentofChemistry,UniversityofCalifornia,Berkeley,California,USA
WeiyingLin
StateKeyLaboratoryofChemo/BiosensingandChemometrics,CollegeofChemistryand
ChemicalEngineering,HunanUniversity,Changsha,PRChina
HangLu
SchoolofChemical&BiomolecularEngineering,GeorgiaInstituteofTechnology,Atlanta,
Georgia,USA
SergeyLukyanov
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,and
NizhnyNovgorodStateMedicalAcademy,NizhnyNovgorod,Russia
JyotiD.Malhotra
ProteostasisTherapeutics,Cambridge,Massachusetts,USA
*
RalucaMarcu
DepartmentofExperimentalOncology,EuropeanInstituteofOncology,Milan,Italy
H.SusanaMarinho
DepartamentodeQu´ımicaeBioqu´ımicaandCentrodeQu´ımicaeBioqu´ımica,Faculdade
deCieˆncias,UniversidadedeLisboa,Lisboa,Portugal
KseniyaN.Markvicheva
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,Russia
RonaldP.Mason
LaboratoryofToxicologyandPharmacology,NationalInstituteofEnvironmentalHealth
Sciences,NIH,ResearchTrianglePark,NorthCarolina,USA
ZimpleMatharu
DepartmentofBiomedicalEngineering,UniversityofCalifornia,Davis,California,USA
MikhailE.Matlashov
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,Russia
NataliyaM.Mishina
Shemyakin-OvchinnikovInstituteofBioorganicChemistry,RAS,Moscow,and
NizhnyNovgorodStateMedicalAcademy,NizhnyNovgorod,Russia
ToshiakiMiura
GraduateSchoolofPharmaceuticalSciences,HokkaidoUniversity,Sapporo,Japan
MikaMorikawa
KagawaSchoolofPharmaceuticalSciences,TokushimaBunriUniversity,Sanuki,Japan
RyuichiOkada
KagawaSchoolofPharmaceuticalSciences,TokushimaBunriUniversity,Sanuki,Japan
FrancescoPaolucci
DepartmentofChemistry,UniversityofBologna,“G.Ciamician”,Bologna,Italy
*
Presentaddress:MitochondriaandMetabolismCenter,Seattle,Washington,USA
xiv Contributors
IrinaV.Perevoschikova
TheBuckInstituteforResearchonAging,Novato,California,USA
CaseyL.Quinlan
TheBuckInstituteforResearchonAging,Novato,California,USA
StefaniaRapino
DepartmentofExperimentalOncology,EuropeanInstituteofOncology,Milan,Italy
AlexanderRevzin
DepartmentofBiomedicalEngineering,UniversityofCalifornia,Davis,California,USA
CarstenSchultz
EMBLHeidelberg,Heidelberg,Germany
MarinaV.Shirmanova
NizhnyNovgorodStateMedicalAcademy,NizhnyNovgorod,Russia
AdamSikora
DepartmentofBiophysicsandFreeRadicalResearchCenter,MedicalCollegeofWisconsin,
Milwaukee,Wisconsin,USA,andInstituteofAppliedRadiationChemistry,Lodz
UniversityofTechnology,Lodz,Poland
FionaA.Summers
LaboratoryofToxicologyandPharmacology,NationalInstituteofEnvironmentalHealth
Sciences,NIH,ResearchTrianglePark,NorthCarolina,USA
SatoshiWatabe
BLCo.,Ltd.,Numazu,Japan
LinYuan
StateKeyLaboratoryofChemo/BiosensingandChemometrics,CollegeofChemistryand
ChemicalEngineering,HunanUniversity,Changsha,PRChina
ElenaV.Zagaynova
NizhnyNovgorodStateMedicalAcademy,NizhnyNovgorod,Russia
BaozhongZhao
SectionofDermatology,DepartmentofMedicine,UniversityofChicago,Chicago,Illinois,
USA
KaiboZheng
StateKeyLaboratoryofChemo/BiosensingandChemometrics,CollegeofChemistryand
ChemicalEngineering,HunanUniversity,Changsha,PRChina
SasaZhu
StateKeyLaboratoryofChemo/BiosensingandChemometrics,CollegeofChemistryand
ChemicalEngineering,HunanUniversity,Changsha,PRChina
JacekZielonka
DepartmentofBiophysicsandFreeRadicalResearchCenter,MedicalCollegeofWisconsin,
Milwaukee,Wisconsin,USA
PREFACE
The identification of hydrogen peroxide in regulation of cell signaling and
geneexpressionwasasignificantbreakthroughinoxygenbiology.Hydro-
genperoxideisprobablythemostimportantredoxsignalingmoleculethat,
among others, can activate NFkB, Nrf2, and other universal transcription
factors and is involved in the regulation of insulin and MAPK signaling.
These pleiotropic effects of hydrogen peroxide are largely accounted for
bychangesinthethiol/disulfidestatusofthecell,animportantdeterminant
ofthecell’sredoxstatus.Moreover,disruptionofredoxsignalingandcon-
trol recognizes the occurrence of compartmentalized cell redox circuits.
Hydrogen peroxide signaling has been of central importance in cell
researchforsometime,andsomepreviousvolumesofMethodsinEnzymology
havecoveredinpartsomeaspectsofthephysiologicalrolesofhydrogenper-
oxide. However, there have been new developments and techniques that
warrantthesethreevolumesofMethodsinEnzymology,whichweredesigned
tobethepremierplaceforacompendiumofhydrogenperoxidedetection
and delivery methods, microdomain imaging, and determinants of hydro-
genperoxidesteady-statelevels;inaddition,theroleofhydrogenperoxide
incellularprocessesentailingredoxregulationofcellsignalingandtranscrip-
tion was covered by experts in mammalian and plant biochemistry and
physiology.
Inbringingthisvolumetofruition,creditmustbegiventotheexpertsin
various aspects of hydrogen peroxide signaling research, whose thorough
and innovative work is the basis of these three Methods in Enzymology vol-
umes. Special thanks to the Advisory Board Members—Christopher
J. Chang, So Goo Rhee, and Balyanaraman Kalyanaram—who provided
guidanceintheselectionoftopicsandcontributors.Wehopethatthesevol-
umeswouldbeofhelptobothnewandestablishedinvestigatorsinthisfield.
ENRIQUE CADENAS
LESTER PACKER
May 2013
xv
METHODS IN ENZYMOLOGY
VOLUME I. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME II. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME III. Preparation and Assay of Substrates
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME V. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME VI. Preparation and Assay of Enzymes (Continued)
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME VII. Cumulative Subject Index
Edited by SIDNEY P. COLOWICK AND NATHAN O. KAPLAN
VOLUME VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD AND VICTOR GINSBURG
VOLUME IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited by RONALD W. ESTABROOK AND MAYNARD E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
VOLUME XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
VOLUME XIII. Citric Acid Cycle
Edited by J. M. LOWENSTEIN
VOLUME XIV. Lipids
Edited by J. M. LOWENSTEIN
xvii
xviii MethodsinEnzymology
VOLUME XV. Steroids and Terpenoids
Edited by RAYMOND B. CLAYTON
VOLUME XVI. Fast Reactions
Edited by KENNETH KUSTIN
VOLUME XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR AND CELIA WHITE TABOR
VOLUME XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DONALD B. MCCORMICK AND LEMUEL D. WRIGHT
VOLUME XIX. Proteolytic Enzymes
Edited by GERTRUDE E. PERLMANN AND LASZLO LORAND
VOLUME XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE MOLDAVE AND LAWRENCE GROSSMAN
VOLUME XXI. Nucleic Acids (Part D)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
VOLUME XXII. Enzyme Purification and Related Techniques
Edited by WILLIAM B. JAKOBY
VOLUME XXIII. Photosynthesis (Part A)
Edited by ANTHONY SAN PIETRO
VOLUME XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by ANTHONY SAN PIETRO
VOLUME XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS AND SERGE N. TIMASHEFF
VOLUME XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS AND SERGE N. TIMASHEFF
VOLUME XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS AND SERGE N. TIMASHEFF
VOLUME XXVIII. Complex Carbohydrates (Part B)
Edited by VICTOR GINSBURG
VOLUME XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
VOLUME XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE MOLDAVE AND LAWRENCE GROSSMAN
VOLUME XXXI. Biomembranes (Part A)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
MethodsinEnzymology xix
VOLUME XXXII. Biomembranes (Part B)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
VOLUME XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS AND EDWARD A. DENNIS
VOLUME XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. JAKOBY AND MEIR WILCHEK
VOLUME XXXV. Lipids (Part B)
Edited by JOHN M. LOWENSTEIN
VOLUME XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O’MALLEY AND JOEL G. HARDMAN
VOLUME XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O’MALLEY AND JOEL G. HARDMAN
VOLUME XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN AND BERT W. O’MALLEY
VOLUME XXXIX. Hormone Action (Part D: Isolated Cells, Tissues,
and Organ Systems)
Edited by JOEL G. HARDMAN AND BERT W. O’MALLEY
VOLUME XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O’MALLEY AND JOEL G. HARDMAN
VOLUME XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. WOOD
VOLUME XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. WOOD
VOLUME XLIII. Antibiotics
Edited by JOHN H. HASH
VOLUME XLIV. Immobilized Enzymes
Edited by KLAUS MOSBACH
VOLUME XLV. Proteolytic Enzymes (Part B)
Edited by LASZLO LORAND
VOLUME XLVI. Affinity Labeling
Edited by WILLIAM B. JAKOBY AND MEIR WILCHEK
VOLUME XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS AND SERGE N. TIMASHEFF
