Hydrocarbons and Methylotrophy

563 Pages·1990·9.441 MB·English

by  Mary E. Lidstrom (Eds.)

Contributors to Volume 188
elcitrA numbers are in sesehtnerap gniwollof the names of contributors.
Aff~fions listed are .tnerruc
REHPOTSIRHC ANTHONY (33, 34, 42, 44), ,smsinagroorciM ts1 Dorozhni proezd ,1
Department of Biochemistry, The -revinU Moscow ,545311 U.S.S.R.
sity of Southampton, Southampton S09
HOWARD DALTON (30), Department of -oiB
5TU, England
lacigol Sciences, University of ,kciwraW
DAVID M. ARCIERO (l 5), Department of -eG yrtnevoC CV4 7AL, England
netics and Cell ,ygoloiB University of Min-
ROTCIV L. DAVIDSON (38), Department of
,atosen St. Paul, Minnesota 80155
Biochemistry, University of Mississippi
N. ARFMAN (35, 60), Department of Micro- Medical Center, Jackson, Mississippi
,ygoloib University of ,negninorG 9751 NN 61293
Haren, The sdnalrehteN
DARREN J. DAY (33, 44), Department of
YGGEP J. ARPS (59), Institute of Arctic -loiB
Biochemistry, The University of South-
ogy, University of Alaska at Fairbanks,
,notpma Southampton, S09 5TU, England
,sknabriaF Alaska 57799
WIM ED KONING (67, 68), Department of
W. ASHRAF (5, 6), Department of lacigoloiB
Biochemistry, B.C.P. Jansen Institute,
,secneicS University of Warwick, Coventry
ytisrevinU of Amsterdam, 1018 TV Am-
CV4 7AL, England
sterdam, The sdnalrehteN
MARGARET M. ATTWOOD (47), Department
of Molecular Biology and ,ygolonhcetoiB L. DIJKHUIZEN (35, 60, 63), Department of
ytisrevinU of ,dleiffehS Sheffield SIO 2TN, ,ygoloiborciM University of ,negninorG
England 1579 NN Haren, The sdnalrehteN
WOLFGANG BABEL (52, 53, 62, 69), Institut ALAN A. DISPIRITO (43), Department of Bi-
f~r eigolonhcetoiB der Akademie der Wis- ,ygolo University of Texas at ,notgnilrA
,neifahcsnes Leipzig ,0507 German Demo- ,notgnilrA Texas 91067
citarc Republic
J. A. DUINE (7, 32, 37, 41, 45, 48), Kluyver
DAVID P. BALLOU (11), Department of -oiB yrotarobaL of ,ygolonhcetoiB Delft -revinU
lacigol Chemistry, University of ,nagihciM sity of ,ygolonhceT 2628 BC Delft, The
Ann Arbor, Michigan 48109 sdnalrehteN
CHRISTOPHER J. BATIE (11), Department of TRUB D. ENSLEY (9), Envirogen Inc.,
Biochemistry and Molecular Biology,
,ellivecnerwaL New Jersey 84680
Louisiana State University Medical
,retneC New ,snaelrO Louisiana 21007 BARRIE ENTSCH (24), Department of Bio-
chemistry, Microbiology and Nutrition,
DINOEL V. HKYRTSYB (60, 65, 67, 68), Insti-
University of New England, Armidale,
tute of Biochemistry and Physiology of
N.S. .W 2351, Australia
Microorganisms, U.S.S.R. Academy of
Sciences, Pushchino, Moscow ,292241 ATREBOR L. FARRELL (27), Repligen San-
.R.S.S.U doz Research Corporation, Lexington,
Massachusetts 37120
CARL E. CERNIGLIA (25), Microbiology Di-
,noisiv National Center for Toxicology Re- W. R. FINNERTY (2, 3, 4), Finnerty Enter-
,hcraes Food and Drug Administration, prises Inc., Athens, aigroeG 50603
,nosreffeJ Arkansas 97027 BRIAN G. FOX (31), Department of -mehcoiB
ANDREY Y. VODRESOTSIHC (39, 50), Insti- ,yrtsi Medical School, University of Min-
tute of sciteneG and Selection of Industrial ,atosen Minneapolis, Minnesota 55455
xi
xii CONTRIBUTORS TO VOLUME 188
J. FRANK (32, 37, 41, 45), Kluyver Labora- Microbiology, University of Iowa, Iowa
tory of Biotechnology, Delft University of City, Iowa 52242
Technology, 2628 BC Delft, The Nether- YERFFOEG W. DOOWYAH (66), County Ana-
lands lyst's Laboratory Fillingate, Wanlip, Lei-
WAYNE A. FROLAND (31), tnemtrapeD Jo cester LE7 8PF, England
Biochemistry, Medical School, University LEAHCIM A. PMAKTIEH (25), Environmen-
of Minnesota, Minneapolis, Minnesota tal Sciences Center, Monsanto Company,
55455
St. Louis, Missouri 63167
PHILIP J. GEARY (10, 23), Shell Research KLAUS H. HOFMANN (69), Institutfi~rAnge-
Ltd., Sittingbourne Research Centre, Sit- wandte und Technische Mikrobiologie,
tingbourne, Kent ME9 8AG, England Ernst-Moritz-Arndt-Universitdt, Greifs-
ANNAHOJ F. RELSSIEG (26), Pharmacology wald, 2200, German Democratic Republic
Division, CIBA-GEIGY AG, CH-4056 RAHZAM HUSAIN (46), Department of Ap-
Basel, Switzerland plied Immunochemistry, Diagnostics Divi-
ENAJ NOSBIG (26), Section of Biochemistry, sion, Abbott Laboratories, Abbott Park, Il-
Molecular and Cell Biology, Cornell Uni- linois 60064
versity, Ithaca, New York 14853
SIRHC L. JOANNOU (10), Department of Mi-
L. A. AVELVOLOG (19), Institute of Bio- crobiology, Kings College London, London
chemistry and Physiology of Microorga- W8 7AH, England
nisms, U.S.S.R. Academy of Sciences, DIVAD R. JOLLIE (31, 49), Department of
Pushchino, Moscow 142292, U.S.S.R.
Biochemistry, Medical School, University
PATRICIAM. NlWDOOG (55), School of Cell of Minnesota, Minneapolis, Minnesota
and Molecular Biology, North East Surrey 55455
College of Technology, Ewell, Epson, Sur- J. A. NAJEGNOJ (41), Kluyver Laboratory of
rey KT17 3DS, England Biotechnology, Delft University of Tech-
N. I. ANIHKUROVOG (60), Institute of Bio- nology, 2628 BC Delft, The Netherlands
chemistry and Physiology of Microorga- B. NAMARANAYLAK (27), Department of
nisms, U.S.S.R. Academy of Sciences, Radiology, Medical College of Wisconsin,
Pushchino, Moscow 142292, U.S.S.R. Milwaukee, Wisconsin 63226
B. W. GROEN (7, 41), Kluyver Laboratory of I. A. KATAEVA (19), Institute of Biochemis-
Biotechnology, Delft University of Tech- try and Physiology of Microorganisms,
nology, 2628 BC Delft, The Netherlands U.S.S.R. Academy of Sciences, Pushchino,
A. J. HACKING (58), Dextra Laboratories Moscow 142292, U.S.S.R.
Ltd., Reading, Berks RG6 2BX, England Noauo KATO (61, 70, 71), Department of
YLLIB E. RELGIAH (9), HQ AFESC/RDVW, Biotechnology, Faculty of Engineering,
Tyndall Air Force Base, Panama City, Tottori University, Koyama-Cho, Tottori
Florida 32403 680, Japan
KENNETH E. HAMMEL (27), Department of SAERDNA G. SIDOPOTAK (1), tnemtrapeD of
Chemistry, S.U.N.Y. College of Environ- Chemistry and Biochemistry, Georgia In-
mental Science and Forestry, Syracuse, stitute of Technology, Atlanta, Georgia
New York 13210 30332
WIN HARDER (65, 67, 68), MT-TNO Delft, PHiLiP J. KERSTEN (27), Biotechnology
2600 AE Delft, The Netherlands Center, University of Wisconsin--
MARK R. HARPEL (17, 18), Department of Madison, Madison, Wisconsin 53705
Biochemistry, Medical School, University LEAHCIM Y. N1HKUIRIK (39, 50), Institute of
of Minnesota, Minneapolis, Minnesota Genetics and Selection of Industrial Micro-
55455 organisms, 1st Dorozhni proezd ,1 Mos-
ENILORAC S. DOOWRAH (26), Department of cow 113545, U.S.S.R.
CONTRIBUTORS TO VOLUME 188 xiii
T. KENT KIRK (27), Institute for Microbial DRAHCIR B. REHGAEM (21, 22), C/P -eG
and Biochemical ,ygolonhceT .U S. Forest netics Department, University of ,aigroeG
stcudorP ,yrotarobaL Madison, Wisconsin Athens, aigroeG 20603
50735
RAMTEID MIETHE (52), Institutf~r -hcetoiB
ALIMDUL V. AVOSTELK (50), Institute of -eG eigolon der Akademie der ,netfahcsnessiW
netics and Selection of Industrial -roorciM Leipzig ,0507 German Democratic Repub-
ganisms, ts1 Dorozhni proezd ,1 Moscow lic
,545311 U.S.S.R.
AHIBAS MOZAFFAR (72), Laboratory ofln-
DORIS BUATS-RELHOK (54), Mikrobiolo- dustrial Biochemistry, Department of In-
gisches Institut, EidgenOssische Tech- dustrial Chemistry, Faculty of -reenignE
,ehcsin Hochschule, ETH-Zentrum, LFV, ,gni Kyoto ,ytisrevinU Yoshida, Sakyo-ku,
CH-8092 Zftrich, Switzerland Kyoto 606, Japan
CINDER KREMA (57), Department of -loiB DNALOR H. RELLDIM (62), Institute of -DiB
ogy, University of Texas at ,notgnilrA Ar- ,ygolonhcet Academy of Sciences of the
,notgnil Texas 91067 German Democratic Republic, Leipzig
RETEP J. LARGE (66), Department of Applied ,0507 German Democratic Republic
,ygoloiB The University of Hull, Hull HU6 ELEIRBAG TFARK-RELLOM (53), Institutfi~r
7RX, England eigolonhcetoiB der Akademie der Wissen-
SAMOHT LEISlNGER (54), sehcsigoloiborkiM ,netfahcs Leipzig 7050, German Demo-
Institut, Eidgen6ssische Technische, citarc Republic
Hochschule, ETH-Zentrum, CH-8092 J. C. LLERRUM (5, 6), Department of -goloiB
,hcirtfZ Switzerland ical ,secneicS University of Warwick, -voC
P. R. GNIREVEL (63), lacigoloiborciM R & D entry CV4 7AL, England
Labs, Organon International B. ,.V 5340
NELLE L. NEIDLE (20), Department of Mi-
BH Oss, The sdnalrehteN
,ygoloiborc University of Texas Medical
YRAM E. MORTSDIL (56, 57), Environmental ,loohcS Houston, Texas 52277
Engineering Science, California Institute
KA-LEUNG NGAI (20, 2 l, 22), Department of
of Technology, Pasadena, California
,yrtsimehcoiB Molecular Biology and Cell
52119
,ygoloiB Northwestern University, Evans-
JOHN D. BMOCSPIL (14, ,51 16, 17, 18, 31, ,not Illinois 80206
49), Department of ,yrtsimehcoiB Medical
School, University of Minnesota, Minne- L. SALOHCIN NOTSNRO (20, 2 I, 22), -trapeD
,silopa Minnesota 55455 ment of Biology, Yale University, New
,nevaH tucitcennoC 11560
YNOTNA R. LONG (34), Department of -DiB
chemistry, The University of Southamp- ALLEN M. ORVILLE (14, 15, 18), -trapeD
,not Southampton S09 5 ,UT England ment of Biochemistry, Medical ,loohcS
University of Minnesota, Minneapolis,
YMEREJ R. MASON (10, 23), Department of
Minnesota 55455
Microbiology, Kings College London,
London W8 7AH, England NOMIS J. PILKINGTON (30), Department of
lacigoloiB Sciences, University of War-
NODLEHS W. MAY (1), Department of
,kciw Coventry CV4 7AL, England
Chemistry and Biochemistry, aigroeG In-
stitute of ,ygolonhceT Atlanta, Georgia J. R. ELYAUQ (58), University of Bath, Bath
23303 BA2 4.7 ,Y England
MAILLIW S. MCINTIRE (36, 40), Department A. P. VOLOKOS (51), Institute of -simehcoiB
of Veterans Affairs, Medical Center, Mo- try and Physiology of ,smsinagroorciM
lecular Biology Division, San ,ocsicnarF .R.S.S.U Academy of ,secneicS ,onihchsuP
ainrofilaC 12149 Moscow ,292241 U.S.S.R.
xiv CONTRIBUTORS TO VOLUME 188
OUSTA TANAKA (28, 29, 72), Laboratory of R. A. NAV RED MEER (41), -arobaLrevyulK
Industrial Biochemistry, Department of tory of ,ygolonhcetoiB Delft ytisrevinU of
Industrial ,yrtsimehC Faculty of -reenignE ,ygolonhceT 2628 BC Delft, The -rehteN
,gni Kyoto ,ytisrevinU Yoshida, Sakyo-ku, sdnal
Kyoto 606, Japan
M. A. G. NAV KLEEF (41), Kluyver -arobaL
MING TIEN (27), Department of raluceloM tory of ,ygolonhcetoiB Delft ytisrevinU of
and Cell ,ygoloiB ainavlysnneP State -inU
,ygolonhceT 2628 BC Delft, The -rehteN
,ytisrev University Park, ainavlysnneP
sdnal
20861
Y. A. OKNESTORT (51), Institute of Bio- NHOJ E. NAV WmLINK (37), Kluyver Labo-
chemistry and Physiology of -agroorciM yrotar of ,ygolonhcetoiB Delft ytisrevinU of
nisms, U.S.S.R. Academy of ,secneicS ,ygolonhceT 2628 BC Delft, The -rehteN
,onihchsuP Moscow ,292241 U.S.S.R. sdnal
RETEP W. TRUDGILL (12, 13), tnemtrapeD
MARTEN VEENHUIS (64), Laboratory for
of Biochemistry, University College of
nortcelE ,ypocsorciM Biological ,retneC
,selaW Aberystwyth, Dyfed SY23 3DD,
ytisrevinU of ,negninorG 9751 AA ,neraH
selaW
The sdnalrehteN
1RUY D. VOKNAGYST (39, 50), Institute of
sciteneG and noitceleS of lairtsudnI -orciM ECNERWAL P. TTEKCAW (8), Department of
,smsinagro ts1 Dorozhni proezd ,1 Mos- ,yrtsimehcoiB Gray retawhserF lacigoloiB
woc ,545311 U.S.S.R. Institute, University of Minnesota, -aN
IHSOYUSTIM UEDA (28, 29, 72), yrotarobaL ,errav Minnesota 29355
of Industrial ,yrtsimehcoiB Department of
SEMAJ W. REKATTIHW (14), Department of
Industrial ,yrtsimehC Faculty of -reenignE
,yrtsimehC nolleM-eigenraC ,ytisrevinU
,gni Kyoto ,ytisrevinU Yoshida, Sakyo-ku,
,hgrubsttiP ainavlysnneP 31251
otoyK 606, Japan
ADI J. NAV RED KLEI (64, 65), tnemtrapeD DROFNAS A. WOLGEL (16), Department of
of ,ygoloiborciM Biological ,retneC Uni- ,yrtsimehcoiB Medical School, ytisrevinU
ytisrev of ,negninorG 9751 NN ,neraH The of Minnesota, Minneapolis, Minnesota
sdnalrehteN 55455
Preface
In the past decade there has been an explosion of interest in organisms
that grow on one-carbon compounds and in those that grow on higher
hydrocarbons. This is a result of the commercial interest in the unique
enzymes involved in these specialty metabolic pathways and to a growing
understanding of the important role these organisms play in carbon cycling
in nature. More recent interest in the use of these organisms and their
enzymes for detoxification of hazardous waste has once again put the
spotlight on these unique microorganisms. It seems clear that the study of
bacteria involved in the utilization of one-carbon compounds and hydro-
carbons is only beginning to realize its potential.
As in all biological systems, the ultimate understanding and exploita-
tion of properties are highly dependent on information concerning the
enzymes involved. This volume is the first comprehensive compilation of
methods for the assay and purification of the enzymes involved in the
utilization of reduced one-carbon compounds and higher hydrocarbons. In
many cases, improved assay and purification methods are presented, while
in others purification is reported for the first time.
In organizing and editing this volume, I have attempted to produce a
source that will have value for researchers in academia, government, and
industry alike. The information presented should provide a quick and
handy reference guide for anyone with interest in these enzymes.
YRAM E. MORTSDIL
XV
METHODS IN ENZYMOLOGY
VOLUME .I Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME II. Preparation and Assay of Enzymes
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME III. Preparation and Assay of Substrates
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME IV. Special Techniques for the Enzymologist
Edited by SIDNEY P. KCIWOLOC DNA NATHAN O. KAPLAN
VOLUME V. Preparation and Assay of Enzymes
Edited by SIDNEY P. COLOWlCK DNA NATHAN O. KAPLAN
VOLUME VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME VII. Cumulative Subject Index
Edited by SIDNEY P. COLOWICK DNA NATHAN O. KAPLAN
VOLUME VIII. Complex Carbohydrates
Edited by HTEBAZILE F. NEUFELD DNA GINSBURG VICTOR
VOLUME IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA MAYNARD E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
VOLUME XII. Nucleic Acids A (Parts and B)
Edited by LAWRENCE GROSSMAN AND KIVIE MOLDAVE
iivx
xviii METHODS IN ENZYMOLOGY
EMULOV XIII. Citric Acid Cycle
Edited by J. M. NIETSNEWOL
EMULOV XIV. Lipids
Edited by J. M. NIETSNEWOL
EMULOV XV. Steroids and Terpenoids
Edited by DNOMYAR .B NOTYALC
EMULOV XVI. Fast Reactions
Edited by HTENNEK NITSUK
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and )B
Edited by TREBREH ROBAT DNA AILEC ETIHW ROBAT
EMULOV XVIII. Vitamins and Coenzymes (Parts A, ,B and C)
Edited by DLANOD .B KCIMROCcM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited by EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by EIVOI EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA EIVIK EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW .B YBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part )B
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part )B
Edited by C. H. W. SRIH DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by .C H. W. SRIH DNA EORES N. FFEHSAMIT
METHODS IN ENZYMOLOGY xix
EMULOV XXVII. Enzyme Structure (Part D)
detidE yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part )B
detidE yb ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
detidE yb ECNERWAL NAMSSORG DNA EIVIK EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
detidE yb ElVIK EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
detidE yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXII. Biomembranes (Part )B
detidE yb YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
detidE yb AHTRAM G. SINNED DNA DRAWDE A. SINNED
EMULOV XXXIV. Affinity Techniques (Enzyme Purification: Part )B
detidE yb MAILLIW .B YBOKAJ DNA MEIR KEHCLIW
EMULOV XXXV. Lipids (Part )B
detidE yb NHOJ M. NIETSNEWOL
EMULOV XXXVI. Hormone Action (Part A: Steroid Hormones)
detidE yb TREB W. YELLAM'O DNA LEOJ G. NAMDRAH
EMULOV XXXVII. Hormone Action (Part :B Peptide Hormones)
detidE yb TREB W. YELLAM'O DNA LEOJ G. NAMDRAH
EMULOV XXXVIII. Hormone Action (Part :C Cyclic Nucleotides)
detidE yb LEOJ G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
detidE yb LEOJ G. NAMDRAH DNA TREB W. YELLAM'O
EMULOV XL. Hormone Action (Part :E Nuclear Structure and Function)
detidE yb TREB W. YELLAM'O DNA LEOJ G. NAMDRAH
XX METHODS IN YGOLOMYZNE
EMULOV XLI. Carbohydrate Metabolism (Part B)
Edited by W. A. DOOW
EMULOV XLII. Carbohydrate Metabolism (Part C)
Edited by W. A. DOOW
EMULOV XLIII. Antibiotics
Edited by JOHN H. HSAH
EMULOV XLIV. Immobilized Enzymes
Edited by SUALK HCABSOM
EMULOV XLV. Proteolytic Enzymes (Part B)
by Edited OLZSAL DNAROL
EMULOV XLVI. Affinity Labeling
Edited by MAILLIW B. YBOKAJ DNA MEIR KEHCLIW
EMULOV XLVII. Enzyme Structure (Part E)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLVIII. Enzyme Structure (Part F)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XLIX. Enzyme Structure (Part G)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV L. Complex Carbohydrates (Part C)
Edited by ROTCIV ~RUBSNIG
EMULOV LI. Purine and Pyrimidine NucleotideM etabolism
Edited by PATRICIA A. HOFFEE AND MARY ELLEN JONES
EMULOV LiI. Biomembranes (Part C: Biological Oxidations)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
EMULOV LIII. Biomembranes (Part D: Biological Oxidations)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LIV. Biomembranes (Part E: Biological Oxidations)
Edited by SIDNEY FLEISCHER AND LESTER PACKER
METHODS IN ENZYMOLOGY xxi
EMULOV LV. Biomembranes (Part F: Bioenergetics)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LVI. Biomembranes (Part G: Bioenergetics)
Edited by YENDIS REHCSIELF DNA RETSEL REKCAP
EMULOV LVII. Bioluminescence and Chemiluminescence
Edited by ENELRAM A. ACULED
EMULOV LVIII. Cell Culture
Edited by MAILLIW .B YBOKAJ DNA ARI NATSAP
EMULOV LIX. Nucleic Acids and Protein Synthesis (Part G)
Edited by ElVIK EVADLOM DNA ECNERWAL NAMSSORG
EMULOV LX. Nucleic Acids and Protein Synthesis (Part H)
Edited by ElVIK EVADLOM DNA ECNERWAL NAMSSORG
EMULOV .16 Enzyme Structure (Part H)
Edited by C. H. .W SRIH DNA EGRES N. FFEHSAMIT
EMULOV .26 Vitamins and Coenzymes (Part D)
Edited by DLANOD .B KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV .36 Enzyme Kinetics and Mechanism (Part :A Initial Rate and
Inhibitor Methods)
Edited by LEINAD L. HCIRUP
EMULOV .46 Enzyme Kinetics and Mechanism (Part :B Isotopic Probes
and Complex Enzyme Systems)
Edited by LEINAD L. HCIRUP
EMULOV 65. Nucleic Acids (Part I)
Edited by ECNERWAL NAMSSORG DNA EIVIK EVADLOM
EMULOV .66 Vitamins and Coenzymes (Part E)
Edited by DLANOD .B KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV .76 Vitamins and Coenzymes (Part F)
Edited by DLANOD .B KCIMROCCM DNA LEUMEL D. THGIRW