Table Of ContentContributors to Volume 109
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
GRETI AGUILERA (10), Endocrinology and JOHN J. M. NOREGREB (18), Department of
Reproduction Research Branch, National Anatomy, McGill University Medical
Institute of Child Health and Human De- School, Montreal, Quebec H3A 2B2,
velopment, National Institutes of Health, Canada
Bethesda, Maryland 50202
L. REMUABNRIB (1, 38, 45), Department of
G. AILHAUD (33), Centre de Biochimie ud Cell Biology, Baylor College of Medicine,
CNRS, Faculte des Sciences, Parc Houston, Texas 03077
Valrose, 06034 Nice C~dex, France
P. F. EROMKCALB (43), Howard Hughes
CLAUDE D. ARNAUD (4, 5), Department of Medical Institute, and the Department of
Medicine, Veterans Administration Medi- Physiology, Vanderbilt University School
cal Center, and Departments of Medicine of Medicine, Nashville, Tennessee 23273
and Physiology, University of ,ainrofilaC DANIEL F. BOWEN-PoPE (8), Department of
San Francisco, California 12149 Pathology, School of Medicine, Univer-
sity of Washington, Seattle, Washington
GILBERT ASHWELL (19), National Institute
59189
of Arthritis, Diabetes, and Digestive and
Kidney Diseases, National Institutes of YRRAB L. BROWN (24), Department of Hu-
Health, Bethesda, Maryland 50202 man Metabolism and Clinical Biochemis-
try., University of Sheffield Medical
ALBERT BAUKAL (10), Endocrinology and
School, Sheffield OIS 2RX, England
Reproduction Research Branch, National
Institute of Child Health and Human -eD MICHAEL S. BROWN (36), Department of
velopment, National Institutes of Health, Molecular Genetics, University of Texas
Bethesda, Maryland 50202 Health Science Center at Dallas, Dallas,
Texas 53257
ERNST BAYER (26), Physiologisch-Chem-
isches Institut der Universitiit, D-8700 NASUS A. BUHROW (62), Department of -iB
Wiirzburg, Federal Republic of Germany ological Chemistry, ehT John Hopkins
University School of Medicine, Balti-
SUZANNE K. RENKCEB (30, 31), Laboratory
more, Maryland 50212
of Cellular and Developmental Biology,
National Institute of Arthritis, Diabetes, TREBOR E. CANFIELD (50), Department of
Digestive and Kidney Diseases, National Medicine, Columbia University College
Institutes of Health, Bethesda, Maryland of Physicians and Surgeons, New York,
50202 New York 23001
HAROLD R. BEHRMAN (25), Reproductive MAHARG CARPENTER (9), Department of
Biology Section, Department of Obstet- Biochemistry and Division of Dermatol-
rics and Gynecology and Pharmacology, ogy, Vanderbilt University School of
Yale University School of Medicine, New Medicine, Nashville, Tennessee 23273
Haven, Connecticut 01560
KEVIN J. CATT (10), Endocrinology and Re-
YHTOMIT P. BENDER (55), National Cancer production Research Branch, National
Institute~Navy Medical Oncology Institute of Child Health and Human De-
Branch, Naval Hospital, Bethesda, Mary- velopment, National Institutes of Health,
land 41802 Bethesda, Maryland 50202
xi
xii CONTRIBUTORS TO VOLUME 109
JANICE YANG CHOU (34), Human Genetics ELLEBASI TRUOF-RETNASUD (52), Labora-
Branch, National Institute of Child Physiologie de toire al ed Lactation, Insti-
Health and Human Development, Na- tut National ed al Recherche Agrono-
tional Institutes of Health, Bethesda, , mique CNRZ, 78350 Jouy-en-Josas,
Maryland 50202 France
ALEX N. (11), EBERLE Laboratory of Endo-
JUAN CODINA (38, 45), Department of Cell
crinology, Department of Research, Uni-
Biology, Baylor College of Medicine,
versity Hospital and University Chil-
Houston, Texas 03077
dren s" Hospital, CH-4031 Basel,
ORDEP SASACERTAUC (35), Department of Switzerland
Molecular Biology, ehT Wellcome Re-
PAUL H. EHRLICH (50), Sandoz Research
search Laboratories, Research Triangle
Institute, Sandoz Inc., East Hanover,
Park, North Carolina 90772
New Jersey 63970
MICHAEL P. CZECH (14), Department of HPESOJ GREBHCIE (41), Department of Bio-
Biochemistry, University of Massachu- chemical and Biophysical Sciences, Uni-
setts Medical School, Worcester, Massa- versity of Houston, Houston, Texas 40077
chusetts 50610 W. n. EVANS (20), National Institute for
KCIREDERF J. RELERAD (30, 31), Laboratory Medical Research, Mill Hill, London
of Cellular and Developmental Biology, NW7 IAA, England
National Institute of Arthritis, Diabetes, J. H. (43), EXTON Howard Hughes Medical
Digestive and Kidney Diseases, National Institute, and the Department of Physiol-
Institutes of Health, Bethesda, Maryland ogy, Vanderbilt University School of
5O2O2 Medicine, Nashville, Tennessee 23273
WILLIAM n. YADAHGUAD (59), Metabolism J. N. FAIN (39), Section of Biochemistry,
Division, Department of Medicine, Wash- Division of Biology and Medicine, Brown
ington University School of Medicine, St. University, Providence, Rhode Island
Louis, Missouri 01136 29120
ERREIP N. E. ED GRAAN (ll), Division of DAVID R. FERRY (42), RudolfBuchheim-ln-
stitut fiir Pharmakologie, Justus Liebig
Neurobiology, Rudolf Magnus Institute
Universitiit, Giessen, D-63 Giessen, Fed-
for Pharmacology, and Institute of Mo-
eral Republic of Germany
Biology, lecular State University Utrecht,
NL-3508 BT Utrecht, ehT Netherlands JAMES B. FIELD (45), Division of -ircodnE
nology, Department of Medicine, Baylor
TREBOR B. NOSKCID (21), Medical Breast
College of Medicine, Houston, Texas
Cancer Section, Medicine Branch, Divi-
03077
sion of Cancer Treatment, National Can-
cer Institute, National Institutes of SECNARF M. FINN (37), Protein Research
Health, Bethesda, Maryland 50202 Laboratory, University of Pittsburgh,
School of Medicine, Pittsburgh, Pennsyl-
JEAN (12, DJIANE 52), Laboratoire ed Phys-
vania 16251
iologie ed al Lactation, lnstitut National
de al Recherche Agronomiqae, CNZR, C. FOREST (33), Centre de Biochimie du
05387 Jouy-en-Josas, France CNRS, Faculte des Sciences, Parc
Valrose, 06034 Nice Cgdex, France
PAULINE R. M. NOSBOD (24, 63), Depart-
ment of Human Metabolism and Clinical H. G. (54), FRIESEN Department of Physiol-
Biochemistry, University of Sheffield ogy, Faculty of Medicine, University of
Medical School, Sheffield OIS 2RX, En- Manitoba, Winnipeg, Manitoba R3E
gland 0W3, Canada
CONTRIBUTORS TO VOLUME 109 xiii
YRREJ D. GARDNER (7, 23), Digestive Dis- KLAUS H. HOFMANN (37), Protein Re-
eases Branch, National Institute of Ar- search Laboratory, University of Pitts-
thritis, Diabetes, and Digestive and Kid- burgh, School of Medicine, Pittsburgh,
ney Diseases, National Institutes of Pennsylvania 16251
Health, Bethesda, Maryland 50202
LOUIS-MARIE HOUDEBINE (52), Labora-
SAMOHT GLASER (26), Troponwerke, Neuro- toire de Physiologie de al Lactation, lnsti-
biology Department, D-5000 K61n, Fed- tut National de al Recherche Agrono-
eral Republic of Germany mique, CNRZ, 78350 Jouy-en-Josas,
TUMTRAH NNAMSSOLG (10, 42), lnstitutfiir France
Biochemische Pharmakologie, A-6020 ~INER E. HUMBEL (60), Biochemisches ln-
Innsbruck, Austria
stitut, University of Zarich, CH-8057 Za-
HPESOJ L. GOLDSTEIN (36), Department of rich, Switzerland
Molecular Genetics, University of Texas
RAVl RAGNEYI (2, 17), Department of Cell
Health Science Center at Dallas, Dallas,
Biology, Baylor College of Medicine,
Texas 53257
Houston, Texas 03077
P. GRIMALDI (33), Centre de Biochimie du NEVETS SBOCAJ (35), Department of Molec-
CNRS, Faculte des Sciences, Parc raM Biology, The Wellcome Research
Valrose, 06034 Nice Cddex, France Laboratories, Research Triangle Park,
BERND HAMPRECHT (26, 27), Physio- North Carolina 90772
logisch-Chemisches lnstitut der Universi- DRANOEL JARETT (15), Department of Pa-
tdt, D-8700 Wiirzburg, Federal Republic thology and Laboratory Medicine, Uni-
of Germany versity of Pennsylvania, Philadelphia,
JOHN A. REVONAH (21), Laboratory of Mo- Pennsylvania 40191
lecular Biology, National Cancer Insti- ELORAC L. JELSEMA (40), Laboratory of
tute, National Institutes of Health, Be- Cell Biology, National Institutes of Men-
thesda, Maryland 50202 tal Health, National Institutes of Health,
JOE DROFRAH (19), National Institute of Ar- Bethesda, Maryland 50202
thritis, Diabetes, and Digestive and Kid- TREBOR T. JENSEN (7, 23), Digestive Dis-
ney Diseases, National Institutes of eases Branch, National Institute of Ar-
Health, Bethesda, Maryland 50202 thritis, Diabetes, and Digestive and Kid-
SELRAHC A. NOTGNIRRAH (41), Analytical ney Diseases, National Institutes of
Neurochemistry Laboratory, Texas Re- Health, Bethesda, Maryland 50202
search Institute of Mental Sciences, EAHNI 1J (16), Department of Biochemistry,
Houston, Texas 03077 University of Wyoming, Laramie, Wyo-
Jose A. HEDO (47), Diabetes Branch, Na- ming 17028
tional Institute of Arthritis, Diabetes, -iD TAE H. JI (16), Department of Biochemistry,
gestive and Kidney Diseases, National University of Wyoming, Laramie, Wyo-
Institutes of Health, Bethesda, Maryland ming 17028
5O2O2
DRANOEL R. NOSNHOJ (6), Department of
JOHN T. HERBERG (2, 17), Department of Physiology and Cell Biology, University
Cell Biology, Baylor College of Medicine, of Texas Medical School, Houston, Texas
Houston, Texas 03077 52077
JOHN D. HIEDEBRANDT (38, 45), Worcester KRAM L. NOSNHOJ (46), Department of Bio-
Foundation for Experimental Biology, chemistry, St. Jude Children's Research
Schrewsbury, Massachusetts 54510 Hospital, Memphis, Tennessee 10183
xiv CONTRIBUTORS TO VOLUME 109
C. RONALD KAHN (47, 48), Research Divi- ECINREB SLEUMAS-SUCRAM (51), Diabetes
sion, Joslin Diabetes Center, and Depart- Branch, National Institute of Arthritis,
ment of Medicine, Brigham and Wom- Diabetes, Digestive and Kidney Diseases,
en's Hospital, and Harvard Medical National Institutes of Health, Bethesda,
School, Boston, Massachusetts 51220 Maryland 50202
OTASAM AGUSAK (48), Department Third of IDA K. MARIZ (59), Metabolism Division,
Internal Medicine, School of Medicine, Department of Medicine, Washington
University of Tokyo, Tokyo, Japan University School of Medicine, St. Louis,
OASAM KATOH (12, 52), Laboratory of Mo- Missouri 01136
lecular Endocrinology, Royal Victoria
JOAN ~IUGASSAM (14), Department of Bio-
Hospital, Montreal H3A IA1 Quebec,
chemistry, University of Massachusetts
Canada
Medical School, Worcester, Massachu-
PaUL A. KELLY (12, 52), Laboratory of Mo- setts 50610
lecular Endocrinology, Royal Victoria
Hospital, Montreal H3A IAl Quebec, ARETTAM LEAEAR (45), Department of Cell
adanaC Biology, Baylor College of Medicine,
Houston, Texas 03077
DOOSAM N. KHAN (18), Department of
Medicine, McGill University Medical J. MAXWELL MCKENZIE (53), Department
School, Montreal, Quebec H3A IAl, of Medicine, University of Miami School
Canada of Medicine, Miami, Florida 10133
C. YELRIHS LIN (49), Department of Immu-
R. MOLENAAR (22), Department of Bio-
nology and Inflammation Research,
chemistry H, Medical Faculty, Erasmus
Merck Sharp Dohme and Research Labo-
University, 3000 DR Rotterdam, ehT
ratories, Rahway, New Jersey 56070
Netherlands
MICHAEL C. LIN (30, 31), Laboratory of
Cellular and Developmental Biology, Na- JOEL Moss (40), Laboratory of Me- Cellular
tional Institute of Arthritis, Diabetes, -iD tabolism, National Heart, Lung, and
gestive and Kidney Diseases, National Blood Institute, National Institutes of
Institutes of Health, Bethesda, Maryland Health, Bethesda, Maryland 50202
50202 WILLIAM R. MOYLE (50), Department of
FRIDOLIN LOFFLER (27), Physiologisch- Obstetrics and Gynecology, University of
Chemisches Institut der Universitiit, -D Medicine and Dentistry of New Jersey,
0078 Federal Republic Wiirzburg, of -reG Piscataway, New Jersey 45880
many
R. NEGREL (33), Centre de Biochimie du
JUDITH L. LUBORSgY (25), Reproductive Bi- CNRS, Faculte des Sciences, Parc
ology Section, Department of Obstetrics Valrose, 06034 Nice Cddex, France
and Gynecology, Yale University School
of Medicine, New Haven, Connecticut TREBOR A. NOSNESSIN (4, 5), Department
01560 of Medicine, Veterans Administration
Medical Center, and University of -ilaC
VINCENT C. OLLEINAGNAM (40), Labora-
fornia, San Francisco, California 12149
tory of Cellular Metabolism, National
Heart, Lung, and Blood Institute, Na- SAMUEL R. NUSSBAUM (49), Endocrine
tional Institutes of Health, Bethesda, Unit, Massachusetts General Hospital,
Maryland 50202 and Harvard Medical School, Boston,
Massachusetts 41120
P. MANJUNATH (56), Reproduction Re-
search Laboratory, Research Clinical In- SAMOHT F. SNOSRAP (57), International -eG
stitute of Montreal, Montreal, Quebec netic Engineering, Inc., Santa Monica,
H2W IR7, Canada California 40409
CONTRIBUTORS TO VOLUME 109 XV
ARI PASTAN (21), Laboratory of Molecular ALAN S. LAHTNESOR (49), Department of
Biology, National Cancer Institute, Na- Immunology Inflammation and Research,
tional Institutes of Health, Bethesda, Merck Sharp and Dohme Research Labo-
Maryland 50202 ratories, Rahway, New Jersey 56070
JOHN G. PIERCE (57), Department of Biolog- WALTER LAHTNESOR (38), Department of
ical Chemistry, UCLA School of Medi- Biology, Baylor Cell College of Medicine,
cine, Los Angeles, California 42009 Houston, Texas 03077
LLESSUR Ross (8, 58), Departments of Pa-
YRRAB I. RENSOP (18), Department of Medi-
thology and Biochemistry, School of
cine, McGiU University Medical School,
Medicine, University of Washington, Se-
Montreal, Quebec H3A 1AI, Canada
attle, Washington 59189
JOHN T. POTTS, JR. (49), Massachusetts M. R. (56), SAIRAM Reproduction Research
General Hospital, and Harvard Medical Laboratory, Research Institute Clinical of
School and Medical Services, Boston, Montreal, Montreal, Quebec H2W IR7,
Massachusetts 41120 Canada
HCIRDEIRF PROPST (26), Frederick Cancer IT1KNES SAKAI (12), Department of Animal
Research Facility, Frederick, Maryland Breeding, Faculty of Agriculture, Univer-
10712 sity of Tokyo, Tokyo Japan 113,
ELAINE W. RAINES (58), Department of Pa- O. M. SALMON (32), Hormones Division,
thology, University of Washington, Seat- National Institute for Biological Stan-
tle, Washington 59189 dards and Control, Holly Hill, Hamp-
stead, London NW3 6RB, England
GEORG REISER (26), Physiologisch-Chem-
DRANREB P. (29), SCHIMMER Banting and
isches lnstitut der Universit6t, D-7400
Best Department of Medical Research,
Tiibingen, Federal Republic of Germany
University of Toronto, Toronto, Ontario
ODNANREF A. P. RmEIRO-NETO (45), Divi- MSG IL6, Canada
sion of Endocrinology, Department of
GNAGELOW J. REDIENHCS (36), Department
Medicine, Baylor College of Medicine,
of Molecular Genetics, University of
Houston, Texas 03077
Texas Health Science Center at Dallas,
JOHN R. RODGERS (46), Howard Hughes Dallas, Texas 53257
Medical Institute, Baylor College of Med- LEAHCIM MMARHCS (28), Department of -iB
icine, Houston, Texas 03077 ological Chemistry, ehT Hebrew Univer-
OCSICNARF J. ROJAS (1), Department of -bO sity of Jerusalem, 40919 Jerusalem, Israel
stetrics and Gynecology, University of ECYOJ A. REORHCS (55), Laboratory oflm-
Texas Health Sciences Center at San An- munogenetics, National Institute of Al-
tonio, San Antonio, Texas 48287 lergy and Infectious Diseases, National
Institutes of Health, Bethesda, Maryland
F. F. G. STREMMOR (22), Department of
502O2
Biochemistry H, Medical Faculty, Eras-
mus University, 3000 DR Rotterdam, ehT D. SCHULSTER (32), Hormones Division,
Netherlands National Institute for Biological Stan-
dards and Control, Holly Hill, Hamp-
YERFFEJ M. NESOR (46), Department of UeC stead, London NW3 6RB, England
Biology, Baylor College of Medicine,
DLANOR D. (38, SEKURA 44, 45), Labora-
Houston, Texas 03077
tory of Developmental and Molecular Im-
MICHAEL TTALBNESOR (49), Biological Re- munity, National Institute of Health Child
search, Merck Sharp and Dohme Re- and Human Development, National Insti-
search Laboratories, West Point, Penn- tutes of Health, Bethesda, Maryland
sylvania 68491 50202
xvi CONTRIBUTORS TO VOLUME 109
ERIC M. SHOOTER (3), Department of Neu- RONALD D. VALE (3), Department of Neu-
robiology, Stanford University School of robiology, Stanford University School of
Medicine, Stanford, California 50349 Medicine, Stanford, California 50349
J. S. A. SIMPSON (54), Diagnostic Assays
H. J. NAV RED MOLEN (22), Department of
Department, Hazleton Biotechnologies
Biochemistry H, Medical Faculty, Eras-
Corporation, Vienna, Virginia 08122
mus University, 3000 DR Rotterdam, ehT
ROBERT M. SMITH (15), Department of Pa- Netherlands
thology and Laboratory Medicine, Uni-
versity of Pennsylvania, Philadelphia, JUDSON J. VAN WYK (61), Department of
Pennsylvania 40191 Pediatrics, Division of Pediatric Endocri-
nology, University of North Carolina
JAMES V. STAROS (62), Department of Bio-
School of Medicine, Chapel Hill, North
chemistry, Vanderbilt University, School
Carolina 41572
of Medicine, Nashville, Tennessee 23273
SONIA STEINER (28), Department of -igoloiB M. A. WALLACE (39), Section of Biochemis-
cal Chemistry, ehT Hebrew University of try, Division of Biology and Medicine,
Jerusalem, 40919 Jerusalem, Israel Brown University, Providence, Rhode Is-
PHILIP G. STRANGE (63), Department of land 21920
Biochemistry, ehT Medical School,
MORRIS F. WHITE (48), Research Division,
Queen's Medical Center, Nottingham
Joslin Diabetes Center, Boston, Massa-
NG7 2UH, England
chusetts 51220
THOMAS W. STR1CKLAND (57), AMGen,
Thousand Oaks, California 02319 MARK C. WILLINGHAM (21), Laboratory of
Molecular Biology, National Cancer In-
MARJORIE E. SVOBODA (61), Department of
stitute, National Institutes of Health, Be-
Pediatrics, Division of Pediatric Endocri-
thesda, Maryland 50202
nology, University of North Carolina
School of Medicine, Chapel Hill, North CLEMENT W. T. YEUNG (13), Playfair
Carolina 41572 Neuroscience Unit and Department of
SIMEON .1 TAYLOR (51), Diabetes Branch, Biochemistry, University of Toronto, -oT
National Institute of Arthritis, Diabetes, ronto, Ontario M5T 2S8, Canada
Digestive and Kidney Diseases, National
CECIL C. YIP (13), Banting and Best Depart-
Institutes of Health, Bethesda, Maryland
ment of Medical Research, University of
50202
Toronto, Toronto, Ontario M5G IL6,
ANNE P. TEITELBAUM (4, 5), Department of Canada
Medicine, University of California, San
Francisco, California 12149 MARGARITA ZAKARIJA (53), Department of
Medicine, University of Miami School of
LISA H. UNDERHILL (51), Diabetes Branch,
Medicine, Miami, Florida 10133
National Institutes of Arthritis, Diabetes,
Digestive and Kidney Diseases, National PETER P. ZUMSTEIN (60), Dana-Farber
Institutes of Health, Bethesda, Maryland Cancer Institute, Boston, Massachusetts
50202 51120
Preface
The field of hormone action is undoubtedly one of the fastest growing
areas of biological science. A rough assessment of the rate of growth of
this field, as determined from an evaluation of journal articles and pro-
grams of national meetings, leads us to the surprising conclusion that an
approximate tenfold expansion has occurred over the last decade. Re-
search in hormone action not only has grown into a dominant effort in
endocrinology and reproductive biology, but has also captured a large
share of the more general disciplines of biochemistry, cell biology, and
molecular biology. This development has occurred because of the dy-
namic aspects of the field and the increasing interest inherent in the new
discipline of regulatory biology. None of these advances could have oc-
curred without a widespread concurrent development of new techniques
or adaptation of relevant techniques from other disciplines for studies on
hormones and the mechanisms involved in hormone action.
In this volume of Methods ni Enzymology a series of techniques and
methods of study as they relate to research on peptide hormones and their
mechanisms of action have been compiled. It has been subdivided into
sections on receptor assays, identification of receptor proteins on cell
surfaces, methods for the identification of internalized hormones and hor-
mone receptors, preparation of hormonally responsive cells and cell hy-
brids, purification of membrane receptors, assays for hormonal effects
and related functions, the use of antibodies in the study of hormone
action, and, finally, into a section on general methods which includes a
variety of methods for the modification of protein hormones, purification,
some of the newer growth factors, as well as other methods of interest.
As always, the techniques gathered are not all-inclusive, some of the
research areas are presented in a fragmentary way, and, undoubtedly,
important methods have escaped our attention. We hope, however, that
the approaches and methods that we have collected in this volume will be,
as they have already been, of very general applicability and an aid both to
researchers and to the more rapid advancement of the field of hormones
and hormone action.
LUTZ BIRNBAUMER
BERT W. O'MALLEY
xvii
METHODS IN ENZYMOLOGY
EDITED BY
Sidney P. Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
I. Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
V. Preparation and Assay of Enzymes
VI. Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
xix
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick and Nathan O. Kaplan
VOLUME VIII. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD DNA VICTOR GRUBSNIG
VOLUME IX. Carbohydrate Metabolism
Edited yb WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited by RONALD W. KOORBATSE DNA DRANYAM E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRs
VOLUME XII. Nucleic Acids (Parts A and B)
Edited yb LAWRENCE NAMSSORG DNA KIVIE EVADLOM
VOLUME XIII. Citric Acid Cycle
Edited by J. M. LOWENSTEIN
VOLUME XIV. Lipids
Edited by J. M. LOWENSTEIN
VOLUME XV. Steroids and Terpenoids
Edited by RAYMOND B. NOTYALC
VOLUME XVI. Fast Reactions
Edited by KENNETH KUSTIN
EMULOV XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR AND CELIA WHITE TABOR
VOLUME XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited by DONALD B. MCCORMICK DNA LEMUEL D. WRIGHT
VOLUME X1X. Proteolytic Enzymes
Edited by GERTRUDE E. PERLMANN DNA LORAND LASZLO
xxi
xxii
METHODS lN ENZYMOLOGY
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited yb WILLIAM B. YBOKAJ
EMULOV XXIII. Photosynthesis (Part )A
Edited yb YNOHTNA NAS ORTEIP
EMULOV XXIV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited yb C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSN1G
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADEOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited yb KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited yb YENDIS REHCSIELF DNA LESTER REKCAP
EMULOV XXXII. Biomembranes (Part B)
Edited yb REHCSIELF YENDIS DNA LESTER REKCAP
EMULOV XXXIII. Cumulative Subject Index Volumes I-XXX
Edited yb AHTRAM G. SINNED DNA DRAWDE A. SINNED
Description:The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much materia