High affinity ssDNA ligands of HIV-1 reverse transcriptase

54 Pages·2013·2.34 MB·English

‘ USOO5786462A
United States Patent [19] [11] Patent Number: 5,786,462
Schneider et a]. [45] Date of Patent: Jul. 28, 1998
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Attorney, Agent, or Firm—Swanson & Bratschun LLC
UT HER PUBLICATIONS
[57] ABSTRACT
Arnold et a1. (1992) Nature 357:85.
Methods are described for the identi?cation and preparation
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Carey et a1. (1983) Biochemistry 22:2601. of DNA ligands to the HIV-1 reverse transcriptase protein.
Included in the invention are speci?c ssDNA ligands to
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HIV-1 reverse transcriptase identi?ed by the SELEX
Gilboa et al. (1979) Cell 18:93.
method. Also included are ssDNA ligands that inhibit HIV-1
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Irvine et a1. (1991) J. M01. Biol. 2222739. reverse lranscriptase.
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US. Patent Jul. 28, 1998 Sheet 1 of 26 5,786,462
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US. Patent Jul. 28, 1998 Sheet 2 of 26 5,786,462
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US. Patent Jul. 28, 1993 Sheet 3 0f 26 5,786,462
100;
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FIG. 2
US. Patent Jul. 28, 1998 Sheet 4 of 26 5,786,462
WMIGA‘I‘I‘CI‘ - [3533] W WW
L SEQID NO:
24
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66, 83
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US. Patent Jul. 28, 1993 Sheet 5 of 26 5,786,462
31 28
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113 38
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FIG. 3B
US. Patent Jul. 28, 1998 Sheet 6 of 26 5,786,462
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FIG. 4A