Table Of Content1.M. Pajares A.S. Peiia P. Malfertheiner (Eds.)
Helicobacter pylori
and
Gastroduodenal Pathology
With 71 Figures
Springer-Verlag
Berlin Heidelberg New York
London Paris Tokyo
Hong Kong Barcelona
Budapest
Pajares, Jose M., M.D., Ph.D.
Professor of Medicine
Head of Department of Gastroenterology
Hospital de la Princesa
Autonoma University of Madrid
Diego de Leon, 62
28006 Madrid, Spain
Pena, A. Salvador, M.D., Ph.D.
Professor of Gastrointestinal Immunology
Free University of Amsterdam
De Boelelaam 1117
1081 HV Amsterdam, The Netherlands
Malfertheiner, Peter, Prof. Dr. med.
Abt. Innere Medizin II, UniversiHits Klinik Ulm
Robert-Koch-Str. 8
W- 7900 VIm, Germany
ISBN-13:978-3-642-77488-1 e-ISBN-13:978-3-642-77486-7
DOl: 10.1007/978-3-642-77486-7
Library of Congress Cataloging-in-Publication Data. Helicobacter pylori and gastroduodenal
pathology/J.M. Pajares, A.S. Pefia, P. Malfertheiner (eds). p. cm. Includes bibliographical
references and index. 1.
Helicobacter pylori infections-Congresses. I. Pajares, J.M. (Jose M.), 1934-. II. Pefia, A.S. III.
Malfertheiner, P. (Peter), 1950-. QR201.H44H45 1992 616.9'3I--dc20 92-30270
This work is subject to copyright. All rights are reserved, whether the whole or part ofthe material
is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation,
broadcasting, reproduction on microfilm or in other way and storage in data banks. Duplication
of this publication or parts thereof is permitted only under the provisions of theG erman Copyright
Law of September 9, 1965, in its current version, and permission for use must always be obtained
from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law.
© Springer-Verlag Berlin Heidelberg 1993
Softcover reprint of the hardcover 1s t edition 1993
The use of general descriptive names, registered names, trademarks, etc. in this publication does
not imply, even in the absence of a specific statement, that such names are exempt from the
relevant protective laws and regulations and therefore free for general use.
Product liability: The publishers cannot guarantee the accuracy of any information about dosage
and application contained in this book. In every individual case the user must check such
information by consulting the relevant literature.
Typesetting: Macmillan India Ltd., Bangalore-25
19/3130/SPS-5432I O-Printed on acid-free paper
Preface
In 1987, a group of researchers interested in the field of Campylobac
ter pylori and gastroduodenal pathology organized a meeting in
Copenhagen to present and to discuss the current research and
findings on the subject.
This informal meeting was the start of the European Campylo
bacter Study Group (ECSG). When 3 years later,the name Campylo
bacter was changed to Helicobacter the name of the group changed
to EHSG. From its very start this working party has been a
multidisciplinary group with the aim of stimulating research on the
role of H elicobacter in animal and human pathology. One of the
group's commitments is an annual International Workshop: the first
official workshop was held in 1988 in Bordeaux, France, and the next
two followed in 1989 and 1990 in Ulm, Germany, and Toledo, Spain.
The EHSG has published the proceedings of these workshops,
collecting the new data which are generated each year. This third
proceedings book has been named "Helicobacter pylori and Gastro
duodenal Pathology".
We have introduced new sections in this volume dealing with
malignancies and disease in children. Other sections have main
tained their titles, but they contain new data and also give informa
tion on original methods or approaches in basic research and
practical clinical applications. It is not unusual for proceedings
volumes to present data and hypotheses which have not yet been
validated at the time of publication. The editors support this idea,
and feel that the publication of preliminary data will help to
stimulate the interchange of ideas between the scientists involved.
The workshops and the proceeding are the result of the scientific
contributions, the suggestions, and continuous cooperation of the
European Group. The financial support of pharmaceutical and
commercial companies is gratefully acknowledged. We also wish to
express our most sincere appreciation and thanks to the authors,
who have contributed their manuscripts to create this book.
July 1992 The Editors
Contents
I. Microbiology
Helicobacter pylori Detection by Polymerase Chain Reaction
of the Gene Encoding 16S Ribosomal RNA in Fresh
and Paraffin-Embedded Material
S.A. Ho, F.A. Lewis, D.A. Seeker, D.S. Tompkins,
G.R. Taylor, J.I. Wyatt, M.F. Dixon, and P. Quirke
(With 1 Figure). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Polymerase Chain Reaction for Helicobacter pylori.
B. Gobert, J.D. Korwin, M.e. Conroy, M.e. Bene,
and G. e. Faure (With 2 Figures) . . . . . . . . . . . . . . . . . . . . .. 12
Helicobacter pylori Strains from Duodenal Ulcer Patients
Differ at the Genomic Level from Those Patients
with Simple Gastritis
H.H. Yoshimura and D.Y. Graham (With 1 Figure) . . . . . . .. 19
Perspectives on the Microbiology of Helicobacter pylori
F. Megraud . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 22
II. Epidemiology
A Descriptive Study of the Characteristics of People
with H elicobacter pylori Infection
L. Basso, J. Clune, S. Beattie, S. Lawlor,
and C. O'Morain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 27
High Prevalence of Helicobacter pylori-Induced Gastritis
in China: Results of a Screening Study
in 194 Adolescents
R. Raedsch, M. Crespi, R. Waldherr, J. Chang-Claude,
Y.G. Rei, N. Muiioz, and P. Correa (With 2 Figures) . . . . . .. 33
Seroepidemiology of Helicobacter pylori Infection
in Vegans and Meat Eaters
MJ. Webberley, J.M. Webberley, P. Lowe, V. Melikian,
and D. Newell (With 3 Figures). . . . . . . . . . . . . . . . . . . . . . .. 37
VIII Contents
Seroepidemiology of Helicobacter pylori Infection
in the Armed Forces
I. Hammermeister, G. Janus, F. Schamarowski,
M. Rudolf, E. Jacobs, and M. Kist. . . . . . . . . . . . . . . . . . . . .. 41
Helicobacter pylori Infection Clusters in Families
of Healthy Individuals
H.M. Malaty and D.Y. Graham. . . . . . . . . . . . . . . . . . . . . . .. 49
Reinfection with Helicobacter pylori Due to Intrafamilial
Clustering of the Organism
R. Collins, S. Patchett, S. Beattie, C. Keane,
and C. O'Morain (With 2 Figures). . . . . . . . . . . . . . . . . . . . .. 53
Epidemiology: Summary of Workshop and Future Prospects
R.A. Feldman. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 57
III. Pathophysiology
Superficial Components from Helicobacter pylori
That Are Adherent to Epithelial Cells
J.L. Fauchere, M. Boulot Tolle, J.L. Beretti,
and M.J. Blaser (With 4 Figures) . . . . . . . . . . . . . . . . . . . . . .. 65
Bismuth Bioavailability from 205Bi-Labelled Bismuth
Compounds Used in Suppression of Helicobacter pylori
and Treatment of Peptic Ulcer
B. Dresow, R. Fischer, E.E. Gabbe, and H.c. Heinrich
(With 4 Figures). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 73
Optical and Electronic Findings in Helicobacter pylori
Infection of Antral Mucosa
J.D. Delgado Bellido, F. Rivera Hueto, J.J. Rios Martin,
and J.M. Herrerias Gutierrez (With 5 Figures). . . . . . . . . . . .. 82
Bacteria and Gastrin Release
J. Calam, R.A. Goodlad, c.Y. Lee, B. Ratcliffe, M.E. Coates,
G.W.H. Stamp, and N.A. Wright. . . . . . . . . . . . . . . . . . . . . .. 90
Helicobacter pylori, Gastritis, Duodenal Ulcer,
and Gastric Histamine Content
D.M.M. Queiroz, E.N. Mendes, G.A. Rocha,
J.R. Cunha-Melo, A.J.A. Barbosa, G.F. Lima, Jr,
and C.A. Oliveira. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 98
Detection of Phospholipases and Cytotoxic Activities
of Helicobacter pylori
M.A. Daw, R. O'Moore, and C. O'Morain ............... 101
Contents IX
IV. Pathology
Antral Follicular Gastritis Is Characteristic
of H elicobacter pylori Infection
F.J. Sancho, S. Sainz, J. Mones, B. Mirelis, E. Oliva,
and F. Vilardell (With 2 Figures) ....................... 109
Defining the Role of H elicobacter pylori in Relationship
to Relapse of Duodenal Ulcer
R. Logan, R. Polson, M. Walker, P. Gummett, H. Baron,
and G. Misiewicz (With 1 Figure) ...................... 114
Gastritis and Helicobacter pylori Prevalence in Patients
with Heterotopic Gastric Mucosa or Gastric Metaplasia
in the Duodenum
H.K. Koch and S. Boemke ............................ 118
Evolution of Gastritis After Helicobacter pylori Eradication
P. Vincent, H. Lamouliatte, I. Darchis, M. Lecomte,
F. Megraud, and A. Mascarel . . . . . . . . . . . . . . . . . . . . . . . .. 124
Histological Improvements in Gastritis After Eradication
of H elicobacter pylori Infection
R. Petrino, D. Bellis, M. Boero, A. Ponzetto, L. Chiandussi,
and A. Di Napoli (With 1 Figure) . . . . . . . . . . . . . . . . . . . . .. 128
Helicobacter pylori in Subtotal Gastrectomies
D. Boixeda, A.L. San Roman, C. Martin de Argila,
R. Canton, C. Redondo, F. Hernandez Ranz, and L. de Rafael.. 133
Helicobacter pylori and Duodenal Ulcer
C. O'Morain and R. Collins ........................... 136
Helicobacter pylori and the Oesophagus
N. Ectors, K. Geboes, J. Janssens, V. Desmet,
and G. Vantrappen (With 2 Figures) .................... 142
Gastritis: A Short Appraisal of Classification
and the Sydney System
A.B. Price (With 1 Figure) ............................ 151
V. Immunology
Stress Proteins and Local Immune Response
in Helicobacter pylori-Associated Chronic Gastritis
L. Engstrand (With 2 Figures) ........................ , 159
Local and Systemic Antibody Responses During
H elicobacter pylori Infections
A.R. Stacey, P.R. Hawtin,
and D.G. Newell (With 3 Figures) ...................... 165
x
Contents
Local Immunoglobulin A Subclass Alteration
in the Gastric Mucosa of Helicobacter pylori-Infected Patients
M.M.C. van der Est, R.A. Veenendaal, A.S. Pena,
I. Kuiper, and c.B.H.W. Lamers (With 2 Figures) .......... 170
Antibodies Against Helicobacter pylori as an Indicator
of Antrum Gastritis
S.-G. Xu, R.B. Zotz, G. von Recklinghausen, K. Donhuijsen,
and H. Goebell. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 177
Antibodies to Vacuolating Toxin of Helicobacter pylori
in Dyspeptic Patients
N. Figura, M. Bugnoli, P. Guglielmetti, R.A. Musmanno,
M. Russi, S. Quaranta, A. Barberi, A. Rossolini,
and P.F. Bayeli (With 1 Figure) ........................ 181
Cytokines and Mucosal Immune Responses
to H elicobacter pylori
J.E. Crabtree, T.M. Shallcross, R.V. Heatley,
and J.I. Wyatt (With 1 Figure) ......................... 187
Immunological Aspects of Helicobacter pylori Infection
A.S. Pena . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 194
VI. Diagnostic Progress
Usefulness of Two Enzyme-Linked Immunosorbent
Assay Systems for the Diagnosis of Helicobacter pylori-Associated
Gastritis
R.J.L.F. Loffeld, H.L.A. van den Oever,
and E. Stobberingh ................................. 201
VII. Helicobacter pylori and Non-ulcer Dyspepsia
Helicobacter pylori Infection in Gastroesophageal
Reflux Disease
O. Pieramico and P. Malfertheiner ..................... 207
Computerised History Taking for the Assessment
of Helicobacter pylori in Non-ulcer Dyspepsia:
The Quest for the Clinical Syndrome
R.J.L.F. Loffeld and R. Adang (With 1 Figure) ............ 211
VIII. Helicobacter pylori and Malignant Growth
Prevalence of Helicobacter pylori in Patients
with Malignant Growth of Gastric Mucosa
J.M. Pajares, M. Crespo, J. Fraga, P. Sanchez Risco,
A. Lancho, and M. Blanco ............................ 219
Contents XI
A Role for H elicobacter pylori in Gastric Carcinoma?
R.J.L.F. Loffeld, I. Willems,
and J.W. Arends (With 1 Figure) ....................... 224
Increased Incidence of Helicobacter pylori
in Gastric Cancer, as Shown by the Rapid Urease Test
D. Boixeda, A.L. San Roman, C. Martin de Argila,
R. Canton, C. Redondo, L.A. Gil Grande,
and L. de Rafael. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 229
Evaluation of the Presence and Amount
of H elicobacter pylori in Early Gastric Cancer
C. Avellini, V. Cocchi, G.M. Paganelli,
and G. Biasco ...................................... 232
H elicobacter pylori prevalence in Patients
with Severe Dysplasia of Gastric Epithelium
and Associated Pathology
H.K. Koch and K. Grillmeier. . . . . . . . . . . . . . . . . . . . . . . . . . 235
Gastric Cancer and H elicobacter pylori
P. Correa and J. Fox ................................ 239
Role of H elicobacter pylori in Gastric Carcinogenesis:
Future Prospects
P. Correa ......................................... 244
IX. Helicobacter pylori in Children
Epidemiology of Helicobacter pylori Infection in Pediatrics:
A Serologic Study
C. De Giacomo .................................... 247
Prevalence of Antibody of Helicobacter pylori
in Children in Northern Nigeria
C. Holcombe, S. Tsimiri, J. Eldridge, and D.M. Jones
(With 1 Figure) ..................................... 251
Prevalence of Immunoglobulin G Antibodies
to Helicobacter pylori in Mothers of Newborns
with Upper Gastrointestinal Bleeding
O. Sangaletti, R. Tornaghi, M. Petrillo, M. Fontana,
and G. Bianchi Porro (With 1 Figure) ................... 256
Helicobacter pylori Infection in Children
U. Blecker, A. Franckx-Goossens, S. Lauwers,
and Y. Vandenplas .................................. 259
XII Contents
Histological and Serological Examination of H elicobacter pylori
in Estonian Children with Abdominal Complaints
H.-I. Maaroos, I. Nilsson, T. Vorobjova, T. Rago,
and T. Wadstrom (With 2 Figures) ..................... 263
Helicobacter pylori in Children with Recurrent Abdominal Pain
and Other Conditions
C. Camarero, I. Alvarez, L. de Rafael, M. Rodriguez,
H. Escobar, and L. Suarez. . . . . . . . . . . . . . . . . . . . . . . . . . . . 270
Helicobacter pylori-Associated Gastritis in Children
F. Gottrand, K. Francillette, D. Turck, P. Vincent,
M. Lecomte, and J.P. Farriaux ........................ 275
Helicobacter pylori Gastritis in Children: Wide Spectrum
of Symptoms
G. Oderda, E. Poli, P. Lerro, D. Dell'Olio, and N. Ansaldi
(With 1 Figure). .................................... 278
Childhood Peptic Ulcer Can Be Cured by Eradicating
H elicobacter pylori
G. Oderda, M. Forni, I. Morra, F. Altare, D. Dell'Olio,
and N. Ansaldi ..................................... 285
Helicobacter pylori Infection and Gastroduodenal
Pathology in Children
G.Oderda ........................................ 289
X. Treatment
Solubility Characteristics of Different Bismuth Salts
in Gastric Juice
G. Bode, P. Malfertheiner, S. Harle, V. Krivan ,
and H. Ditschuneit (With 3 Figures) .................... 295
Assessment of Susceptibility of Helicobacter pylori
to Tinidazole, Amoxicillin and Tetracycline
Among 555 Patients
D. Fameree, B. Ramdani, and V. Lamy. . . . . . . . . . . . . . . . . . 302
In Vivo Acquired Resistance to Fluorquinolones
in H elicobacter pylori During Therapy with Ciprofloxacin
Alone or Ciprofloxacin plus Amoxicillin Clavulanate
R. Canton, L. de Rafael, D. Boixeda, M.T. Serrano,
M. Garcia-Gonzalez, and F. Baquero (With 1 Figure) ....... 305
Treatment of Helicobacter pylori-Positive Lesions:
13C-Urea Breath Test Monitoring and Morphology
F.E. Bauer, G. Lepsien, I. Meineke, J.V. Geletneky,
A. Schauer, and F.E. Liidtke (With 5 Figures) ............. 312
