Table Of Content•
GROWTH FACTORS
IN REPRODUCTION
SERONO SYMPOSIA, USA
FERTlLlZA TlON IN MAMMALS
Edited by Barry D. Bavister, Jim Cummins, and Eduardo R. S. Roldan
GAMETE PHYSIOLOGY
Edited by Ricardo H. Asch, Jose P. Balmaceda, and Ian Johnston
GL YCOPROTEIN HORMONES: Structure, Synthesis, and
Biologic Function
Edited by William W. Chin and Irving Boime
GROWTH FACTORS IN REPRODUCTION
Edited by David W. Schomberg
THE MENOPAUSE: Biological and Clinical Consequences of
Ovarian Failure: Evaluation and Management
Edited by Stanley G. Korenman
NEUROENDOCRINE REGULA TION OF REPRODUCTION
Edited by Samuel S. C. Yen and Wylie W. Vale
UTERINE CONTRACTILITY: Mechanisms of Control
Edited by Robert E. Garfield
Serono Symposia, USA
Norwell, Massachusetts
GROWTH FACTORS
IN REPRODUCTION
Edited by
DAVID W. SCHOMBERG
Duke University Medical Center
Durham, North Carolina
Springer-Verlag
New York Berlin Heidelberg London
Paris Tokyo Hong Kong Barcelona
David W. Schomberg, PhD
Duke University Medical Center
Durham, NC 27710
USA
Proceedings of the Symposium on Growth Factors in Reproduction, sponsored by Serono Sympo
sia, USA, held April 1 to 4, 1990, in Savannah, Georgia.
For information on previous volumes, please contact Serono Symposia, USA.
Printed on acid-free paper.
© 1991 Springer-Verlag New York, Inc.
Softcover reprint of the hardcover 1st edition 1991
All rights reserved. This work may not be translated or copied in whole or in part without the
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former are not especially identified, is not to be taken as a sign that such names, as understood by
the Trade Marks and Merchandise Marks Act, may accordingly be used freely by anyone.
While the advice and information in this book is believed to be true and accurate at the date of
going to press, neither the authors, nor the editors, nor the publisher, nor Serono Symposia, USA,
nor Serono Laboratories, Inc., can accept any legal responsibility for any errors or omissions that
may be made. The publisher makes no warranty, express or implied, with respect to the material
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CR copy provided by Technical Texts, Inc., Scituate, Massachusetts.
9 8 7 6 543 2 1
ISBN-13: 978-1-4612-7819-1 e-ISBN-13: 978-1-4612-3162-2
DOl: 10.1007/978-1-4612-3162-2
SYMPOSIUM ON GROWTH FACTORS IN REPRODUCTION
Scientific Committee
David W. Schomberg, Ph.D., Chairman
Durham, North Carolina
Eli Y. Adashi, M.D.
Baltimore, Maryland
Robert B. Dickson, Ph.D.
Washington, D.C.
David C. Lee, Ph.D.
Chapel Hill, North Carolina
John A. McLachlan, Ph.D.
Research Triangle Park, North Carolina
R. Michael Roberts, Ph.D.
Columbia, Missouri
Organizing Secretary
L. Lisa Kern, Ph.D.
Serono Symposia, USA
100 Longwater Circle
Norwell, Massachusetts
Preface
The critical role of growth factors in normal as well as abnormal cellular growth and
function has become overwhelmingly apparent in recent years. The significant
advances in reproductive biology within this rapidly expanding field were highlighted
at the Serono Symposia, USA, symposium entitled Growth Factors in Reproduc
tion. The conference focused on growth factors as polyfunctional regulators of
growth and development in the reproductive system.
The program was organized into five areas: (1) growth factors as polyfunctional
regulators of growth and development, (2) growth factors and gonadal function, (3)
regulation of normal and neoplastic mammary growth, (4) regulatory peptides in
reproductive tract development and function, and (5) embryo-maternal signaling.
This volume constitutes the contributions ofthe invited symposium speakers and is
organized into sections representing the five topic areas of the sessions.
Drs. Eli Y. Adashi, Robert B. Dickson, David C. Lee, James Hammond, John A.
McLachlan, and R. Michael Roberts, who served as chairmen ofthese sessions, not
only provided invaluable advice and assistance in organizing the symposium, but
contributed significantly to its content. The enthusiasm and esprit de corps of the
poster presenters and discussion participants also helped make this symposium
succeed as the first of its kind.
Finally, special thanks are due Drs. James Posillico and Lisa Kern ofSerono
Symposia, USA, for their guidance and support in the conception and planning of
the symposium and in the publication of this volume.
David W. Schomberg
vii
Contents
I. Growth Factors: Polyfunctional Regulators of Growth and
Development 1
1 The Epidermal Growth Factor Receptor: Control of Synthesis and Signaling
Function 3
Shelton Earp, William Huckle, Victoria Raymond, Leslie Petch, Sherry Marts,
Warren Bishop, and Bryan McCune
2 Transforming Growth Factor ~: A Multifunctional Regulatory Peptide with
Actions in the Reproductive System 23
Kathleen C. Flanders, Belinda A. Marascalco, Anita B. Roberts, and
Michael B. Sporn
3 Transforming Growth Factor a: Expression and Biological Activities of the
Secreted and Integral Membrane Forms 39
David C. Lee
II. Growth Factors and Gonadal Function 53
4 Growth Factor Regulation of Testicular Function 55
Brian P. Mullaney and Michael K. Skinner
5 Tumor Necrosis Factor a: Localization and Actions Within the Preovulatory
Follicle 63
P. F. Terranova, K. F. Roby, M. Sancho-Tello, J. Weed, and R. Lyles
6 Transforming Growth Factors and Ovarian Function 79
David W. Schomberg and George W. Mulheron
7 Intraovarian IGF-I system 91
Eli Y Adashi, Carol E. Resnick, Eleuterio R. Hernandez, Arye Hunvitz,
Charles T. Roberts, Derek Leroith, and Ron Rosenfeld
III. Regulation of Normal and Neoplastic Mammary Growth 103
8 EGF-Mediated Growth Control and Signal Transduction in the
MDA-MB-468 Human Breast Cancer Cell Line 105
Ronald N. Buick
ix
x Contents
9 Mammary Growth Regulation by Transforming Growth Factor ~ 115
Charles W. Daniel and Gary B. Silberstein
10 Growth Factors as Local Regulators of Normal and Malignant Human
Mammary Epithelium 129
Robert B. Dickson
IV. Regulatory Peptides in Reproductive Tract Development
and Function 149
11 Mullerian Inhibiting Substance Activity in the Development of the
Reproductive Tract and Lung in the Fetus and Control of Oocyte Meiosis
in the Adult 151
David T. MacLaughlin, Tatsuo Kuroda, Elizabeth A. Catlin, and
Patricia K Donahoe
12 Growth Factors Affecting Normal and Malignant Prostatic Cells 167
John T. Isaacs, Ronald A. Morton, Paula Martikainen, and William B. Isaacs
13 Estrogen Regulation of Uterine Epidermal Growth Factor Receptor and
Nuclear Proto-Oncogenes 185
D. S. Loose-Mitchell, R. M. Gardner, S. M. Hyder, J. L. Kirkland, T.-H. Lin,
R. B. Lingham, V. R. Mukku, C. A. Orengo, U. R. Tipnis, and G. M. Stancel
14 Estrogens and Growth Factors in the Development, Growth, and Function of
the Female Reproductive Tract 197
J. A. McLachlan, K G. Nelson, T. Takahash~ N. L. Bossert, R. R. Newbold,
and K S. Korach
V. Embryo-Maternal Signaling 205
15 Expression and Function of Growth Factor Ligands and Receptors in
Preimplantation MouE'-e Embryos 207
Daniel A. Rappolee, Karin S. Sturm, Gilbert A. Schultz, Claudio A. Basilico,
Daniel Bowen-Pope, Roger A. Pedersen, and Zena Werb
16 Colony-Stimulating Factor I in the Mouse and Human Uteroplacental
Unit 219
Jeffrey W. Pollard, Serge Pampfer, Eric Daiter, David Barad, and
Robert J. Arceci
17 Autocrine-Paracrine Role ofLymphohematopoietic Cytokines at the
Maternal-Fetal Interface 231
Thomas G. Wegmann and Larry Guilbert
Contents xi
18 Trophoblast Interferons 235
R. Michael Roberts, James C. Cross, Charlotte E. Farin, Peter W. Farin,
Kyle K. Kramer, Harriet Francis, Clifford Librach, and Susan J. Fisher
Author Index 247
Subject Index 249
PART
I
GROWTH FACTORS:
POLYFUNCTIONAL
REGULATORS OF GROWTH
AND DEVELOPMENT
