Table Of ContentGrowth, Cancer, and the Cell Cycle
The Molecular, Cellular, and Developmental Biology
Experimental Biology and Medicine
Growth, Cancer and the Cell Cycle, edited by Philip
Skehan and Susan J. Friedman, 1984
Ir Genes: Past, Present, and Future, edited by Carl W.
Pierce, Susan E. Cullen, Judith A. Kapp, Benjamin
D. Schwartz, and Donald C. Shreffler, 1983
Methods in Protein Sequence Analysis, edited by
Marshall Elzinga, 1982
Inflammatory Diseases and Copper, edited by John R.
J. Sorenson, 1982
Membrane Fluidity: Biophysical Techniques and Cellular
Regulation, edited by Morris Kates and Amis Kuksis,
1980
GROWTH, CANCER, AND
THE CELL CYCLE
The Molecular, Cellular,
and Developmental Biology
Edited by
PHILIP SKEHAN
and
SUSAN J. FRIEDMAN
The University of Calgary
Calgary, Alberta, Canada
Humana Press . Clifton, New Jersey
DEDICATION
This book is dedicated to the memory of Jacob Duerksen, a friend,
colleague, and fellow member of The International Cell Cycle Society
who contributed much to the organization of this conference. His un
timely death greatly saddened those of us who knew him.
Library of Congress Cataloging in Publication Data
International Cell Cycle Conference (10th: 1984 : Banff
National Park)
Growth, cancer, and the cell cycle.
(Experimental biology and medicine)
Includes index.
1. Cells-Growth-Congresses 2. Cell proliferation
Congresses. 3. Cell cycle-Congresses. 4. Cancer cells
Growth-Congresses. I. Skehan, Philip. II. Friedman,
Susan J. Ill. Title. IV. Series. [DNLM: 1. Cell Cycle.
2. Cell Transformation. Neoplastic. QZ 202 G884]
QH6051525 1984 599'031 84-22468
lSBN-l3: 978-1-4612-9599-0 e-lSBN-l3: 978-1-4612-5178-1
DOl: 10.1007/978-1-4612-5178-1
© 1984 The Humana Press Inc.
Softcover reprint of the hardcover 1st edition 1984
Crescent Manor
PO Box 2148
Clifton, NJ 07015
All rights reserved
No part of this book may be reproduced. stored in a retrieval
system, or transmitted in any form or by any means, electronic,
mechanical, photocopying, microfilming, recording, or otherwise
without written permission from the Publisher.
PREFACE
Cell growth, one of the most fundamental of biological processes,
has long been among the least understood. On April 24-28, 1984 sci
entists convened from around the world in Canada's Banff National
Park for The International Cell Cycle Society's 10th Conference. Their
purpose was to evaluate recent developments in the field of cell prolif
eration and to explore the interrelationship between cell growth, de
velopment, and differentiation, and proliferative diseases such as can
cer. Growth, Cancer, and the Cell Cycle collects those conference
papers that present the most recent advances in this field.
The first section of the book is Gene Expression and Development
During Growth. It examines the structure and function of chromatin,
DNA unwinding proteins, and nonhistone nuclear proteins, then ex
plores transcriptional, translational, and post-translational regulation
during the cell cycle and the interrelationship and coordinate regula
tion of cell growth, differentiation, and gene expression.
The second section, Growth Activation and Dormancy, focuses
upon the events that occur during the transition between active cell
growth and proliferative quiescence. The role of DNA strand breaks,
protein kinase activity, growth regulatory factors, and the cytoskeleton
are examined.
Section three discusses The Topology of the Cell Cycle. It reviews
genetic approaches for determining the sequence of events and cau
sality relationships that comprise and coordinate the many separate
processes involved in cell cycle progression and describes the use of
multipara meter flow cytometry to characterize the mammalian cell cy
cle and intracellular metabolic and transitional growth states.
The final section of the book, Neoplastic Transformation, explores
the role of tumor cell heterogeneity in circulatory metastasis and of ac
tivator RNA in cellular phenotype transformation, presents the latest
advances in the establishment of normal and neoplastic gastrointesti
nal cell lines, describes new methods for cancer diagnosis and of cell
cycle deconvolution by image analysis, and critically evaluates the
concept that cancer is a disease of abnormal cell growth.
Philip Skehan
Susan J. Friedman
v
CONTENTS
Preface ......................................................... v
Acknowledgments .............................................. xiv
List of Contributors ............................................... x
I. GENE EXPRESSION AND DEVELOPMENT DURING
GROWTH
Structures Inherent to Chromatin Active in Transcription and
Replication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. ......... 3
A. T. Annunziato. R. D. Smith. S. K. Hanks. and
R. L. Seale
Chromatin Changes in Skeletal Muscle Cells During the Fusion
to Myotubes ............................................... 27
O. Torok and H. Altmann
A Eucaryotic Protein with Helix Destabilizing Activity and its
Homology to LDH ......................................... 41
Gordhan L. Patel. Sesha Reddigari. Kenneth R.
Williams. Edward Baptist. Peter E. Thompson and
Sangram S. Sisodia
Role of Nonhistone Protein Phosphorylation in the Regulation
of Mitosis in Mammalian Cells. . . . . . . . . . . . . . . . 51
Ramesh C. Adlakha. Chintamin G. Sahasrabuddhe.
David A. Wright. Helene Bigo. and Potu N. Rao
Nonhistone Proteins, Free Radical Defenses and Acceleration of
Spherulation in Physarum.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 71
C. Nations. R. G. Allen. and J. McCarthy
Coordinate and Non-Coordinate Regulation of Histone mRNAs
During Myoblast Growth and Differentiation. . . . . . . . . . . . . . . . . . .. 79
R. Curtis Bird. Fred A. Jacobs. and Bruce H. Sells
The Receptor Binding of Insulin and Poly(ADP-Ribosel
Synthesis During the Cell Cycle . . . . . . . . . . . . . . . . . . . . .. ....... 87
H. Altmann. O. Torok. P. Kovacs. and G. Csaba
A Novel Method of Translation in Fibroin .......................... 99
G. C. Candelas. N. Ortiz. A. Ortiz. T. M. Candelas. and
O. M. Rodnguez
Tubulin and Actin Gene Expression DUring the Cell Cycle ........... 107
S. Zimmerman. A. M. Zimmerman. J. Thomas. and
I. Ginzburg
vii
viii Contents
II. GROWTH ACTIVATION AND DORMANCY
Regulation of Nuclear and Mitochondrial RNA Production After
Serum Stimulation of Quiescent Mouse Cells. . . . . . . . . . . . . . . . .. 117
Dylan R. Edwards and David T. Denhardt
A Decrease in the Steady Level of DNA Strand Breaks as a
Factor in the Regulation of Lymphocyte Proliferation ............ 125
W. L. Greer and J. G. Kaplan
70 K Dalton Protein Synthesis and Heat Sensitivity of
Chromatin Structure: Dependence on Cell Cycle. . . . . . . . . . . . . . . 135
Roeland van Wijk and Wlel Geilenkirchen
Involvement of Protein Kinases in Mitotic-Specific Events ............ 143
Margaret S. Halleck, Katherine Lumley-Sapanskl, Jon
A. Reed, and Robert A. Schlegel
Protein Kinase Activity in Lymphocytes; Effects of Concanavalin
A and A Phorbol Ester ...................................... 151
Anand P. Iyer, Sharon A. Pishak, Marion J. Sniezek, and
Andrea M. Mastro
Effect of Gossypol on DNA Synthesis in Mammalian Cells. . . . . . . . . .. 159
Potu N. Rao, Larry Rosenberg, and Ramesh C. Adlakha
Growth Activation in Adult Rat Hepatocytes ....................... 169
N. L. R. Bucher, W. E. Russell, and J. A. McGowan
Isolation and Characterization of a Growth Regulatory Factor
from 3T3 Cells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 177
John L. Wang and Yen-Mlng Hsu
Changes in AdheSion, Nuclear Anchorage, and Cytoskeleton
During Giant Cell Formation ................................ 187
Susan J. Friedman, Catharine Dewar, James Thomas,
and Philip Skehan
III. TOPOLOGY OF THE CELL CYCLE
The Gl Cell Cycle Interval in Yeasts .............................. 205
R. A. Singer and G. C. Johnston
Coordination of Growth With Cell Division in Yeasts ............... 213
G. C. Johnston and R. A. Singer
Changed Division Response Mutants Function as
Allosuppressors ............................................ 221
Paul G. Young and Peter A. Fantes
Molecular and Cell Biology of Cell Cycle Progression Revealed
by Mammalian Cells Temperature-Sensitive in DNA
Synthesis ................................................. 229
Rose Sheinin
Metabolic and Kinetic Compartments of the Cell Cycle
Distinguished by Multiparameter Flow Cytometry .............. 249
Zbigniew Darzynkiewlcz
Contents ix
IV. NEOPLASTIC TRANSFORMATION
Cancer Cell Heterogeneity in Resistance to Mechanical Trauma
in the Microcirculation as Part of Metastasis. . . . . . . . . . . . . . . . . . . . 281
Helena Gabor
Expression of an "Activator" RNA Hybridizable to the SV40
Promotor Correlates with the Transformed Phenotype of
Mouse and Human Cells .................................... 289
Margarida Krause. Jolanta Kurz. and Uik Sohn
Gastrointestinal Cells: Growth Factors, Transformation, and
Malignancy. . ............................................. 297
M. P. Moyer. P. Dixon. D. Escobar. and J. B. Aust
A New Technique for Diagnosing Cancer by Inspecting Blood
Serum ................................................... 307
A. Kovacs. A. Vertesy. L. Szali. S. Adami.
L. Urbancsek. Z. Simon. G. Nemeth. and
J. M. Takacs
Cytokinetics of Heteroploid Tumor Subpopulations by
Combined Autoradiographic Imaging and Feulgen
Densitometry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 315
Robert J. Sklarew
Cell Growth, Tissue Neogenesis, and Neoplastic
Transformation ............................................ 323
Philip Skehan
Index ........................................................ 347
CONTRIBUTORS
S. ADAMI Cancer Research Group, Pilisborosjeno, Hungary
J. B. AUST . The University of Texas Health Science Center at
San Antonio, San Antonio, Texas
RAMESH C. ADLAKHA Department of Chemotherapy Re-
search, The University of Texas M. D. Anderson Hospital and
Tumor Institute, Houston, Texas
R. G. ALLEN Department of Biology, Southern Methodist Uni-
versity, Dallas, Texas
H. ALTMANN Institute of Biology, Research Center
Seibersdorf, Austria
A. T. ANNUNZIATO Department of Basic and Clinical Re-
search, Scripps Clinic and Research Foundation, La Jolla,
California
EDWARD BAPTIST . Department of Zoology, University of
Georgia, Athens, Georgia
HELENA BIGO Department of Genetics, The University of
Texas M. D. Anderson Hospital and Tumor Institute, Houston,
Texas
R. CURTIS BIRD Department of Molecular Biology and Ge-
netics, College of Biological Science, University of Guelph,
Guelph, Ontario, Canada
N. L. R. BUCHER Department of Pathology, Boston Univer-
sity School of Medicine, Boston, Massachusetts
G. C. CANDELAS Department of Biology, University of
Puerto Rico, Rio Piedras, Puerto Rico
T. M. CANDELAS Department of Biology, University of
Puerto Rico, Rio Piedras, Puerto Rico
G. CSABA Department of Biology, Semmelweis University
School of Medicine, Budapest, Hungary
ZBIGNIEW DARZYNKIEWICZ Memorial Sloan-Kettering Can-
cer Center, New York, New York
DAVID T. DENHARDT . Cancer Research Laboratory, Univer
sity of Western Ontario, London, Ontario, Canada
CATHARINE L. DEWAR . Oncology Research Group and De
partment of Pharmacology, The University of Calgary, Calgary,
Alberta, Canada
x
Contributors xi
P. DIXON The University of Texas Health Science Center at
San Antonio, San Antonio, Texas
DYLAN R. EDWARDS Cancer Research Laboratory, Univer-
sity of Western Ontario. London, Ontario, Canada
D. ESCOBAR . The University of Texas Health Science Center
at San Antonio, San Antonio, Texas
PETER A. FANTES Department of Zoology, University of
Edinburgh, Edinburgh, Scotland
SUSAN J. FRIEDMAN Oncology Research Group and Depart-
ment of Phamacology, The University of Calgary, Calgary,
Alberta, Canada
HELENA GABOR Children's Hospital of Northern California,
Oakland, California.
WIEL GEILENKIRCHEN Department of Zoology, State Uni-
versity, Utrecht, The Netherlands
I. GINZBURG Department of Neurobiology, The Weizmann
Institute of Science, Rehovot, Israel
W. L. GREER Department of Biochemistry, University of
Alberta, Edmonton, Alberta, Canada
MARGARET S. HALLECK . Molecular and Cell Biology Pro
gram, The Pennsylvania State University. University Park.
Pennsylvania
S. K. HANKS Department of Basic and Clinical Research,
Scripps Clinic and Research Foundation. La Jolla, California
YEN-MING HSU Department of Biochemistry. Michigan State
University, East LanSing, Michigan
ANAND P. IYER Microbiology Program, The Pennsylvania
State University, University Park, Pennsylvania.
FRED A. JACOBS Department of Molecular Biology and Ge-
netics. College of Biological Science, University of Guelph.
Guelph, Ontario, Canada
G. C. JOHNSTON Department of Microbiology, Dalhousie
University, Halifax, Nova Scotia, Canada
J. G. KAPLAN Department of Biochemistry, University of
Alberta, Edmonton, Alberta, Canada
A. KovAcs Cancer Research Group, Pilisborosjeno,
Hungary
P. KOVACS Department of Biology, Semmelweis University
School of Medicine, Budapest, Hungary
MARGARIDA KRAUSE Department of Biology, University of
New Brunswick, Fredericton, New Brunswick, Canada
