Table Of ContentCONTENTS
Group photograph of participant ....0......0..0..ccccsecseeeeee Viii—ix Calvin\ Koons, M.D. and Blumenthal, R.M. ................. 73-79
Characterization of pPvul, the autonomous plasmid from
Preface Proteus vulgaris that carries the genes of the Pvull restriction-
Bestor, T.H., Bhagwat, A.S., Blumenthal, R.M., Brooks, J.E., modification system
Marinus, M.G., Raleigh, E.A. and Szybalski, W. Vaisvila, R., Vilkaitis, G. and Janulaitis, A. ................. 81-84
( Eds.) xi Identification of a gene encoding a DNA invertase-like
Proceedings of the Third New England Biolabs Worksho enzyme adjacent to the PaeR7I restriction-modification
on Biological DNA modification system
Proteins
Genes
(a) Purification and characterization
(a) Distribution and diversity Tucholski, J.. Skowron, P.M. and Podhajska, A.J. .... 87-92
Timinskas, A., Butkus, V. and Janulaitis, A. ............... 3-11 Mmel, a class-IIS restriction endonuclease: purification and
Sequence motifs characteristics for DNA [cytosine-N4] and characterization
DNA [adenine-N6] methyltransferases. Classification of all Karyagina, A.S., Lunin, V.G., Levtchenko, LY., Labbé, D.,
DNA methyltransferases Brousseau, R., Lau, P.C.K. and Nikolskaya, LI. .. 93-96
Nyengaard, N., Vogensen, F.K. and Josephsen, J. ..... 13-18 The Ssoll and NlaX DNA methyltransferases: overproduc-
Restriction-modification systems in Lactococcus lactis tion and functional analysis
Stein, D.C., Gunn, J.S., Radlinska, M. and Kupper, D., Reuter, M. and Kriiger, D.H. .................... 97-98
III I ninthc icniinstsctscnesenecrnnencsntincocpapsiitianetis 19-22 Overproduction of His-tagged EcoRII restriction endonucle-
Restriction and modification systems of Neisseria gonorrhoeae ase and terminally deleted mutant proteins
Dedkov, V.S., Rechkunova, N.1., Prihod’ko, E.A., Kileva, E.V.,
(b) Cloning and organization Kusner, Y.S., Verchozina, V.A. and
Lubys, A. and Janulaitis, Ac 0.0.0... ccccccssesseesseeeseennee 25--29 Nl MINI tcstseisnssncibioesiibssiatesttalessnsebbiiidanite 99-100
Cloning and analysis of the plasmid-borne genes encoding CciNI, an isoschizomer of Notl from Curtobacterium citreum
the Bsp6I restriction and modification enzymes recognizes 5’'-GC|GGCCGC-3’
NI MITE: snssssisiitniensicviiiinchianiossinpniiabitesenitentenignsemmaainilis 31-35 Piekarowiecz, A. amd Steien, DOC. .......cccccccosscosccvsccvsssosees 101-102
Cloning of a pair of genes encoding isoschizomeric restriction Purification and characterization of a new DNA methyl-
endonucleases from Bacillus species: the BspEI and BspMII transferase from Neisseria gonorrhoeae
restriction and modification systems Schumann, J., Willert, J., Wild, C., Walter, J. and
Skowron, P.M., Swaminathan, N., McMaster, K., George, D., es IN icc ecicatscascediasiccicninetieeinniniaiiegtiiaaiianes 103-104
Van Etten, J.L. and Mead, D.A. 00.0.0... eeeeeeeenee 37-41 M-BssHII: a new multispecific CS5S-DNA-methyltransferase
Cloning and applications of the two/three-base restriction Szilak, L., Finta, C., Patthy, A., Venetianer, P. and
endonuclease R-CviJI from IL-3A virus-infected Chlorella IAs Aa sca sistcstptaseniesecseisechaiececiniaahaleiaeeiaasbiae 105
Kréger, M., Blum, E., Deppe, E., Diisterhéft, A., Erdmann, D., Self-methylation of the M-BspRI methyltransferase
Kilz, S., Meyer-Rogge, S. and Méstl, D. ................. 43-47 Blum, E., Horst, G. and Kroger, Me ....0..0..0...0.0.000000. 107-108
Organization and gene expression within restriction- PCR-directed preparation and single-step purification of
modification systems of Herpetosiphon giganteus highly active histidine-tagged restriction endonuclease HgiBl
Stankevitius, K., Povilionis, P., Lubys, A., Menkevitius, S. and (GGWCC)
Vitor, J.M.B. and Morgan, R.uD. o0......00...c.cccccecceseeseeeee 109-110
Two novel restriction endonucleases from Campylobacter
modification system comprising two restriction endonucle- jejuni
ases and one methyltransferase
Vaisvila, R., Sliesaravitiite, Z., Kulakauskas, S. and (6) Mutational analysis
Janulaitis, A. . 55-57 Grabowski, G., Jeltsch, A., Wolfes, H., Maass, G. and
Cloning of the ppu211M gene using a in vivo selection method IN acectoric cahartareseidiniatiinsihniaaiaicnasntesticealaaniaiaieiies 113-118
Xu, G., Willert, J., Kapfer, W. and Trautner, T.A. ... 59 Site-directed mutagenesis in the catalytic center of the restric-
BsuCl, a type-I restriction-modification system in Bacillus tion endonuclease EcoRI
subtilis Fisher, E.W., Yang, M.-T., Jeng, S.-t., Gardner, J.F. and
Gopal, J., Yebra, M.J. and Bhagwat, A.S. .................... 61-63 NN cI sipniseessiiinscnitiptecinaldcalihiicasdeepeapmcnias 119-121
Cloning and characterization of the gene encoding the DsaV Selection of mutations altering specificity in restriction-
methyltransferase modification enzymes using the bacteriophage P22 challenge-
Degtyarev, S.K., Prikhod’ko, E.A., Rechkunova, N.L, phage system
Prikhod’ko, G.G. and Krasnykh, V.N. ..................... 65-66 Dryden, D.T.F., Willcock, D.F. and Murray, N.E... 123-124
Biochemical characterization of VspI methyltransferase Mutational analysis of conserved amino-acid motifs in EcoKI
adenine methyltransferase
(c) Mobilization potential Kossykh, V.G., Schlagman, S.L. and Hattman, S....... 125-126
Brassard, S., Paquet, H. and Roy, P.H. .....................0... 69-72 Studies on the function of conserved sequence motifs in the
A transposon-like sequence adjacent to the Accl restriction- T4 Dam-[N®-adenine] and EcoRII [CS5-cytosine] DNA
modification operon methyltransferases
340
Lange, C., Wild, C. and Trautner, T.A. ........................ 127-128 Shivapriya, R., Prasad, R., Narayanan, I.L., Krishnaswamy, S.
Altered sequence recognition specificity of a CS-DNA methyl- and Dharmalingam, K. 201-207
transferase carrying a chimeric ‘target recognizing domain’ Expression of the mcrA gene of Escherichia coli is regulated
post-transcriptionally, possibly by sequestration of the Shine-
(c) Crystal structures Dalgarno region
Schluckebier, G., Labahn, J., Granzin, J., Schildkraut, I. and Lacks, S.A., Greenberg, B. and Sabelnikov, A.G. ....... 209-212
Saenger, W. 131-134 Possible regulation of DNA methyltransferase expression by
A model for DNA binding and enzyme action derived from RNA processing in Streptococcus pneumoniae
crystallographic studies of the Tagl N°-adenine-methyl- Rasmussen, L.J., Lebner-Olesen, A. and
transferase Marinus, M.G. 213-215
O’Gara, M., McCloy, K., Malone, T. and Cheng, X. 135-138 Growth-rate-dependent transcription initiation from the dam
Structure-based sequence alignment of three AdoMet- P2 promoter
dependent DNA methyltransferases RimSeliené, R., VaiSvila, R. and Janulaitis, A. ............. 217-219
Cheng, X., Balendiran, K., Schildkraut, I. and The eco72IC gene specifies a trans-acting factor which influ-
Anderson, J.E. 139-140 ences expression of both DNA methyltransferase and endo-
Crystal structure of the Pvull restriction endonuclease nuclease from the Eco72I restriction-modification system
Fernandez, M., Soliveri, J., Novella, LS., Yebra, M.J.,
DNA-protein interactions Barbés, C. and Sanchez, J. 221-223
Ahmad, I., Krishnamurthy, V. and Rao, D.N. ............. 143-147 Effect of 5-azacytidine and sinefungin on Streptomyces
DNA recognition by the EcoP15I and EcoPI modification development
methyltransferases King, G. and Murray, N.E. 225
Brevnov, M.G., Kubareva, E.A., Romanova, E.A., Volkov, E.M., Modification enhancement and restriction alleviation by bac-
Karyagina, A.S., Nikolskaya, I.1. and teriophage A
Gromova, E.S. 149-152 Sohail, A., Ives, C.L. and Brooks, J.B. 1.0.0... 227-228
Interaction of the Mval and Ssoll methyltransferases with Purification and characterization of C-BamHLI, a regulator of
DNAs altered at the central base pair of the recognition the BamHI restriction-modification system
sequence Kelleher, J.E. and Raleigh, E.A. 229-230
Erskine, S.G. and Halford, S.E. 153-156 On the regulation and diversity of restriction in Escherichia
Interactions of the EcoRV restriction endonuclease with fluo- coli
rescent oligodeoxynucleotides Alvarez, M.A., Gomez, A., Gomez, P. and
Jeltsch, A., Pleckaityte, M., Selent, U., Wolfes, H., Siksnys, V. Rodicio, M.R. 231-232
and Pingoud, A. 157-162 Expression of the Sall restriction-modification system of
Evidence for substrate-assisted catalysis in the DNA cleavage Streptomyces albus G in Escherichia coli
of several restriction endonucleases
Eukaryotic methylation
KlimaSauskas, S. and Roberts, RoJ. ..................ccc:cseee0e- 163-164
(a) Protists
Disruption of the target G-C base-pair by the Hhal
methyltransferase Van Nuland, T.A., Capowski, E.E. and Karrer, K.M. 235-237
Position effect for adenine methylation in the macronuclear
Kriiger, D.H., Kupper, D., Meisel, A., Tierlich, M., Reuter, M.
DNA of Tetrahymena
and Schroeder, C. 165
Restriction endonucleases functionally interacting with two (b) Animals and Man
DNA sites Doerfler, W., Orend, G., Schubbert, R., Fechteler, K.,
Kubareva, E.A., Volkov, E.M., Vinogradova, N.L., Heller, H., Wilgenbus, P. and Schréer, J. ............... 241-245
Kanevsky, 1.A., Oretskaya, T.S., Kuznetsova, S.A., On the insertion of foreign DNA into mammalian genomes:
Brevnov, M.G., Gromova, E.S., Nevinsky, G.A. and mechanism and consequences
Shabarova, Z.A. 167-171 Caiafa, P., Reale, A., Santoro, R., D’Erme, M., Marenzi, S.,
Modified substrates as probes for studying uracil-DNA Zardo, G. and Strom, R. . 247-251
glycosylase Does hypomethylation of linker DNA play a role in chroma-
Petrauskene, O.V., Gromova, E.S., Romanova, E.A., tin condensation?
Volkov, E.M., Oretskaya, T.S. and Strom, R., Santoro, R., D’Erme, M., Mastrantonio, S.,
Shabarova, Z.A. 173-176 Reale, A., Marenzi, S., Zardo, G. and Caiafa, P. 253-256
DNA duplexes containing methylated bases or non- Specific variants of H1 histone regulate CpG methylation in
nucleotide inserts in the recognition site are cleaved by restric- eukaryotic DNA
tion endonuclease R-EcoRII in presence of canonical Gaston, K. and Fried, M. 257-259
substrate CpG methylation and the binding of YY1 and ETS proteins
Renbaum, P. and Razin, A. 177-179
to the Surf-1/Surf-2 bidirectional promoter
Interaction of M-Sss] and M-Hhal with single-base mis- Martin, V., Ribieras, S., Song-Wang, X., Rio, M.-C. and
matched oligodeoxynucleotide duplexes Dante, R. 261-264
Sektas, M., Kaczorowski, T. and Podhajska, A.J. ...... 181-185
Genomic sequencing indicates a correlation between DNA
Interaction of the Mboll restriction endonuclease with DNA hypomethylation in the 5’ region of the pS2 gene and its
Sheflyan, G.Y., Kubareva, E.A., Volkov, E.M., Oretskaya, T.S., expression in human breast cancer cell lines
Gromova, E.S. and Shabarova, Z.A. ......................... 187-190 Jost, J.-P. and Jost, Y.-C. 265-266
Chemical cross-linking of Mval and EcoRI enzymes to DNA Mechanism of active DNA demethylation during embryonic
duplexes containing monosubstituted pyrophosphate inter- development and cellular differentiation in vertebrates
nucleotide bond
(c) Plants
Regulation Nebendahl, A. and Baumlein, H. 269-272
Adams, G.M. and Blumenthal, R.M. ...................... 193-199 Analysis of overlappii.g cDNA clones specific for a putative
Gene pvullW: a possible modulator of Pvull endonuclease second DNA _ methyltransferase-encoding gene in
subunit association Arabidopsis thaliana
341
Ashapkin, V.V., Antoniv, T.T. and Vanyushin, B.F..... 273-277 Piechula, S., Kur, J., Woszezyk, J. and
Methylation-dependent binding of wheat nuclear proteins to NE II dics aseismic 315-316
the promoter region of ribosomal RNA genes Purification and characterization of two restriction endo-
Vlasova, T.I., Demidenko, Z.N., Kirnos, M.D. and nucleases isolated from Phormidium inundatum
Vanyushin, B.F. 279-281 Rutkowska, S.M., Skowron, P.M. and
In vitro DNA methylation by wheat nuclear cytosine DNA BNNs INNIINE dt oiiseeincnsnucnsntetectnsesinensiiactcienisiiaimetonann 317-318
methyltransferase: effect of phytohormones Purification and characterization of the restriction endonucle-
Buryanov, Y.I., Zakharchenko, N.S., Shevchuk, T.V. and ase Avcl from Actinomyces cristalomycini
Bogdarina, I.G. 283-287 Rutkowska, S.M., Skowron, P.M., Bielawski, K. and
Effect of the M-EcoRII methyltransferase-encoding gene on PN I Sastctctssiccctrscasnctlenniethatenannehinitinaisiapaliies 319-320
the phenotype of Nicotiana tabacum transgenic cells SacNI, an isoschizomer of BanII isolated from Streptomyces
Pradhan, S., Houlston, C., Cummings, M. and achromogenes recognizes the 5'-GRGCY/C sequence
Adams, R.L.P. 289-291 Repin, V.E., Lebedev, L.R., Puchkova, L., Serov, G.D.,
CG and CNG methyltransferases in plants Tereschenko, T., Chizikov, V.E. and Andreeva, I.. 321—322
New restriction endonucleases from thermophilic soil bacteria
Other related papers Nomura, Y., Ishino, Y., Kimizuka, F. and Kato, L..... 323-324
Flores, H., Osuna, J., Heitman, J. and Sober6n, X... 295-301 A novel Streptomyces restriction endonuclease, Sse18251,
Saturation mutagenesis of His''* of EcoRI reveals relaxed- cleaving at 5'-GG/GWCCC-3’
specificity mutants Reeben, M., Myéhinen, S., Saarma, M. and
Fomenkov, A. and Xu, S.-ye o.........ccssesssecssesseesseesnessneenneennes 303-310 BPTI SIA sss hcstnsrceseosees ted eeiiinbcina cathepsin 325-329
Isolation of temperature-sensitive mutants of the BamHI Sequencing of the rat light neurofilament promoter reveals
restriction endonuclease differences in methylation between expressing and non-
Siksnys, V., Timinskas, A., KlimaSauskas, S., Butkus, V. and expressing cell lines, but not tissues
Janulaitis, A. 311-314
Sequence similarity among type-II restriction endonucleases, Cartoon
related by their recognized 6-bp target and tetranucleotide-
overhang cleavage
