Gel Electrophoresis - Advanced Techniques

511 Pages·2016·28.04 MB·English

by  Magdeldin S. (Ed.)

GEL ELECTROPHORESIS
ADVANCED TECHNIQUES

Edited by Sameh Magdeldin
GEL ELECTROPHORESIS –
ADVANCED TECHNIQUES

Edited by Sameh Magdeldin
Gel Electrophoresis – Advanced Techniques
Edited by Sameh Magdeldin

Published by InTech
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First published March, 2012
Printed in Croatia

A free online edition of this book is available at www.intechopen.com
Additional hard copies can be obtained from [email protected]

Gel Electrophoresis – Advanced Techniques, Edited by Sameh Magdeldin
p. cm.
ISBN 978-953-51-0457-5

Contents

Preface XI
Part 1 Electrophoresis Application in
Ecological and Biotechnological Aspects 1
Chapter 1 Application of Gel Electrophoresis Techniques to
the Study of Wine Yeast and to Improve Winemaking 3
María Esther Rodríguez, Laureana Rebordinos,
Eugenia Muñoz-Bernal, Francisco Javier Fernández-Acero and
Jesús Manuel Cantoral
Chapter 2 Proteomics in Seaweeds: Ecological Interpretations 21
Loretto Contreras-Porcia and Camilo López-Cristoffanini
Chapter 3 Gel Electrophoresis Based Genetic Fingerprinting
Techniques on Environmental Ecology 51
Zeynep Cetecioglu, Orhan Ince and Bahar Ince
Chapter 4 Gel Electrophoresis of Grapevine
(Vitis vinifera L.) Isozymes – A Review 67
Gizella Jahnke, János Májer and János Remete
Chapter 5 Molecular Electrophoretic Technique for
Authentication of the Fish Genetic Diversity 83
Tsai-Hsin Chiu, Yi-Cheng Su, Hui-Chiu Lin and Chung-Kang Hsu
Chapter 6 Gel Electrophoresis for Investigating
Enzymes with Biotechnological Application 97
Maria de Lourdes T. M. Polizeli, Simone C. Peixoto-Nogueira,
Tony M. da Silva, Alexandre Maller and Hamilton Cabral
Part 2 Electrophoresis Application in Bacteriology, Parasitology,
Mycology and Public Health 111
Chapter 7 Application of Molecular Typing Methods to the
Study of Medically Relevant Gram-Positive Cocci 113
Laura Bonofiglio, Noella Gardella and Marta Mollerach
VI Contents

Chapter 8 Molecular Microbiology Applied to
the Study of Phytopathogenic Fungi 139
Carlos Garrido, Francisco J. Fernández-Acero, María Carbú,
Victoria E. González-Rodríguez, Eva Liñeiro and
Jesús M. Cantoral
Chapter 9 Molecular and Proteolytic Profiles of Trypanosoma cruzi
Sylvatic Isolates from Rio de Janeiro-Brazil 157
Suzete A. O. Gomes, Danielle Misael, Cristina S. Silva,
Denise Feder, Alice H. Ricardo-Silva, André L. S. Santos,
Jacenir R. Santos-Mallet and Teresa Cristina M. Gonçalves
Chapter 10 Pulsed Field Gel Electrophoresis in Molecular
Typing and Epidemiological Detection of Methicillin
Resistant Staphylococcus aureus (MRSA) 179
Velazquez-Meza Maria Elena, Vázquez-Larios Rosario,
Hernández Dueñas Ana Maria and Rivera Martínez Eduardo
Chapter 11 Usefulness of Pulsed Field Gel Electrophoresis
Assay in the Molecular Epidemiological Study of
Extended Spectrum Beta Lactamase Producers 193
Patrick Eberechi Akpaka and Padman Jayaratne
Part 3 Electrophoresis Application in the
Analysis of Protein-Nucleic Acid Interaction
and Chromosomal Replication 203
Chapter 12 Electrophoretic Mobility Shift Assay:
Analyzing Protein – Nucleic Acid Interactions 205
Carolina Alves and Celso Cunha
Chapter 13 Analysis of Chromosomal Replication
Progression by Gel Electrophoresis 229
Elena C. Guzmán and Enrique Viguera
Part 4 Electrophoresis Application in Enzymology 245
Chapter 14 Polyacrylamide Gel Electrophoresis an
Important Tool for the Detection and Analysis
of Enzymatic Activities by Electrophoretic Zymograms 247
Reyna Lucero Camacho Morales, Vanesa Zazueta-Novoa,
Carlos A. Leal-Morales, Alberto Flores Martínez,
Patricia Ponce Noyola and Roberto Zazueta-Sandoval
Chapter 15 Applications of Zymography (Substrate-SDS-PAGE)
for Peptidase Screening in a Post-Genomic Era 265
Claudia M. d’Avila-Levy, André L. S. Santos, Patrícia Cuervo,
José Batista de Jesus and Marta H. Branquinha
Contents VII

Part 5 Temporal Temperature Gel Electrophoresis 289
Chapter 16 Temporal Temperature Gel Electrophoresis
to Survey Pathogenic Bacterial Communities:
The Case of Surgical Site Infections 291
Romano-Bertrand Sara, Parer Sylvie, Lotthé Anne,
Colson Pascal, Albat Bernard and Jumas-Bilak Estelle
Part 6 Two-Dimensional Gel Electrophoresis (2-DE) 313
Chapter 17 Two-Dimensional Gel Electrophoresis Reveals Differential
Protein Expression Between Individual Daphnia 315
Darren J. Bauer, Gary B. Smejkal and W. Kelley Thomas
Chapter 18 Two-Dimensional Gel Electrophoresis
and Mass Spectrometry in Studies of
Nanoparticle-Protein Interactions 327
Helen Karlsson, Stefan Ljunggren, Maria Ahrén,
Bijar Ghafouri, Kajsa Uvdal, Mats Lindahl
and Anders Ljungman
Chapter 19 Two Dimensional Gel
Electrophoresis in Cancer Proteomics 359
Soundarapandian Kannan, Mohanan V. Sujitha,
Shenbagamoorthy Sundarraj and
Ramasamy Thirumurugan
Part 7 Other Applications of Gel Electrophoresis Technique 391
Chapter 20 Enzymatic Staining for Detection of
Phenol-Oxidizing Isozymes Involved in Lignin-
Degradation by Lentinula edodes on Native-PAGE 393
Eiji Tanesaka, Naomi Saeki, Akinori Kochi and
Motonobu Yoshida
Chapter 21 Protection Studies by Antioxidants Using Single Cell Gel
Electrophoresis (Comet Assay) 413
Pınar Erkekoglu
Chapter 22 Gel Electrophoresis as Quality Control Method
of the Radiolabeled Monoclonal Antibodies 447
Veronika Kocurová
Chapter 23 Gel Electrophoresis as a Tool to Study
Polymorphism and Nutritive Value of the
Seed Storage Proteins in the Grain Sorghum 463
Lev Elkonin, Julia Italianskaya and Irina Fadeeva
VIII Contents

Chapter 24 Extraction and Electrophoresis of DNA from
the Remains of Mexican Ancient Populations 479
Maria de Lourdes Muñoz, Mauro Lopez-Armenta,
Miguel Moreno-Galeana, Alvaro Díaz-Badillo,
Gerardo Pérez-Ramirez, Alma Herrera-Salazar,
Elizabeth Mejia-Pérez-Campos, Sergio Gómez-Chávez
and Adrián Martínez-Meza

Preface

“Gel electrophoresis is finding a wide application because its unique separatory power”
Oliver Smithies
Since the first report about gel electrophoresis, released in 1930, and despite the
alternative fractionation technologies that have recently emerged, gel electrophoresis
remains the most popular and most powerful technique that provides a perfect
fractionation of macromolecules (DNA, RNA and Proteins). Hundreds of techniques
could be set according to experimental atmosphere, including various substrates
(agarose or polyacrylamide), gel conditions (native or denaturating), buffers
(TAE, TBE, etc) and others. This broad variability enabled further progression
and development of gel-based fractionation such as isoelectric focusing, zymograms
and 2D-PAGE. With wide application ranging from restriction mapping and
genetic fingerprinting, to forensic application and co-application with blotting system,
there is no doubt that gel electrophoresis made an overwhelming progress in the
“OMICS” era.
It is my pleasure to introduce the book “Gel Electrophoresis - Advanced Techniques”.
This book presents a wide research application of this sophisticated technique with
wider scope to better cover this popular topic.
Sameh Magdeldin, MVSc, PhD (Physiology), PhD (Proteomics)
Senior post doc researcher and Proteomics team leader
Medical School, Niigata University, Japan
Assistant Professor (Lecturer), Physiology Department
Suez Canal University
Egypt