Galectins: Methods and Protocols (Methods in Molecular Biology, 2442)

730 Pages·2022·21.57 MB·English

by  Sean R. Stowell (editor), Connie M. Arthur (editor), Richard D. Cummings (editor)

Methods in
Molecular Biology 2442
Sean R. Stowell
Connie M. Arthur
Richard D. Cummings Editors
Galectins
Methods and Protocols
Second Edition
M M B
ETHODS IN OLECULAR IO LO GY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University ofHertfordshire
Hatfield, Hertfordshire, UK
Forfurther volumes:
http://www.springer.com/series/7651
For over 35 years, biological scientists have come to rely on the research protocols and
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neededtocompletetheexperiment,andfollowedbyadetailedprocedurethatissupported
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Galectins
Methods and Protocols
Second Edition
Edited by
Sean R. Stowell and Connie M. Arthur
Joint Program in Transfusion Medicine Brigham and Women’s Hospital, Harvard Medical School,
Boston, MA, USA
Richard D. Cummings
Department of Surgery Beth Israel Deaconess Medical Center National Center for Functional Glycomics,
Harvard Medical School, Boston, MA, USA
Editors
SeanR.Stowell ConnieM.Arthur
JointPrograminTransfusionMedicine JointPrograminTransfusionMedicine
BrighamandWomen’sHospital BrighamandWomen’sHospital
HarvardMedicalSchool HarvardMedicalSchool
Boston,MA,USA Boston,MA,USA
RichardD.Cummings
DepartmentofSurgeryBethIsrael
DeaconessMedicalCenterNational
CenterforFunctionalGlycomics
HarvardMedicalSchool
Boston,MA,USA
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-0716-2054-0 ISBN978-1-0716-2055-7 (eBook)
https://doi.org/10.1007/978-1-0716-2055-7
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Preface
Galectins:MethodsandProtocols,secondedition,isanupdateofthefirstbooksolelydedicated
to methodological approaches designed to study galectin function. The galectin family
represents one of the most pleiotropic families, with individual members having been
implicatedinvariousaspectsofnearlyeverybiologicalprocessdescribed,fromRNAsplicing
tocomplexregulatorycircuitsthatorchestrateadaptiveimmunity.Giventhediverserolesof
galectins in a variety of biological systems, studying these glycan-binding proteins often
requires the assimilation of diverse technical skills to fully appreciate their biological func-
tion.Theirnearlyubiquitousexpressionandabilitytobindhighlymodifiablecarbohydrate
ligands, in addition to a variety of other regulatory proteins, allows these glycan-binding
proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes.
Individualchaptersarededicatedtoexaminingsalientfeaturesofgalectinfunctions.Written
inthesuccessfulMethodsinMolecularBiologyseriesformat,chaptersincludeintroductions
to their respective topics, lists of the necessary materials and reagents, step-by-step, readily
reproducibleprotocols,andnotesontroubleshootingandavoidingknownpitfalls.
Authoritative and easily accessible,Galectins: MethodsandProtocols seeksto serve both
professionals and novices with a useful framework when examining galectin function for
manyyearstocome.
Boston,MA,USA SeanR.Stowell
ConnieM.Arthur
RichardD.Cummings
v
Acknowledgments
SeanR.Stowell,ConnieM.Arthur,RichardD.Cummings
WewouldliketothankShang-ChuenWu,AnuPaul,KashyapPatel,HansVerkerke,and
AlexD.HoforcriticalreadingofchaptersandfeedbackontheentireGalectins:Methodsand
Protocols,secondedition.
vii
Contents
Preface ..................................................................... v
Acknowledgments............................................................. vii
Contributors................................................................. xiii
1 Galectins:AnAncientFamilyofCarbohydrateBindingProteins
withModernFunctions.................................................. 1
HansVerkerke,MarceloDias-Baruffi,RichardD.Cummings,
ConnieM.Arthur,andSeanR.Stowell
2 Cloning,Expression,andPurificationofGalectins
forInVitroStudies ..................................................... 41
PaulA.Poland,CarolL.Kinlough,andRebeccaP.Hughey
3 PurificationofRecombinantGalectinsfromDifferentSpecies
UsingDistinctAffinityChromatographyMethods.......................... 55
AnuPaul,Shang-ChuenWu,KashyapR.Patel,
AlexD.Ho,JerryWilliamLynnAllen,HansVerkerke,
ConnieM.Arthur,andSeanR.Stowell
4 AlkylationofGalectin-1withIodoacetamideandMassSpectrometric
MappingoftheSitesofIncorporation..................................... 75
Shang-ChuenWu,AnuPaul,RichardD.Cummings,
ChristaL.Feasley,ConnieM.Arthur,andSeanR.Stowell
5 RapidDetectionandPurificationofGalectin-3bytheCapture
andRelease(CaRe)Method.............................................. 89
PriyankaD.Kadav,Jared L.Edwards,JessicaKrycia,
PurnimaBandyopadhyay,andTarunK.Dam
6 Introducing77SeNMRSpectroscopytoAnalyzing
Galectin–LigandInteraction.............................................. 105
Ma´riaRaics,Istva´nTima´ri,La´szl(cid:1)o Szila´gyi,
Hans-JoachimGabius,andKatalinE.K¨ove´r
7 EvaluationofGalectinBindingbySurfacePlasmonResonance............... 125
PadmajaMehta-D’souza
8 RevealingtheIdentityofHumanGalectin-3
asaGlycosaminoglycan-BindingProtein................................... 137
JaredL.Edwards,PriyankaD.Kadav,
PurnimaBandyopadhyay,andTarunK.Dam
9 ExaminingGalectinBindingSpecificityUsingGlycanMicroarrays ............ 151
SeanR.Stowell,LilianC.Rodrigues,MarceloDias-Baruffi,
RichardD.Cummings,andConnieM.Arthur
10 MechanismofMucinRecognitionbyLectins:
AThermodynamicStudy ................................................ 169
TarunK.Dam,Jared L.Edwards,
PriyankaD.Kadav,andC.FredBrewer
ix
x Contents
11 ExaminationofWhole-CellGalectinBindingbySolidPhase
andFlowCytometricAnalysis ............................................ 187
AnneLeppa€nen,ConnieM.Arthur,
SeanR.Stowell,andRichardD.Cummings
12 DissectingContext-SpecificGalectinBinding
UsingGlycoengineeredCellLibraries ..................................... 205
MathiasIngemannNielsenandHansH.Wandall
13 MethodforIdentifyingGalectinLigandsonLymphocyte
MembraneGlycoproteins................................................ 215
KashyapR.Patel,AdamW.Barb,andSeanR.Stowell
14 TransformationofAgrocybecylindraceaGalectin
intoαGalNAc-SpecificLectin............................................. 233
DanHuandJunHirabayashi
15 WhatHappensIfaHumanGalectinEnters
theEndoplasmicReticulum? ............................................. 247
TanjaJ.Kutzner,AlonsoM.Higuero,MartinaSu¨ßmair,
MichaelHingar,HerbertKaltner,IngoLindner,Ju¨rgenKopitz,
Jose´Abad-Rodrı´guez,DietmarReusch,andHans-JoachimGabius
16 InvestigationofGalectinsinFrozenTissueandMammalian
CellCultureUsingConfocalMiccroscopy ................................. 289
DanielGiulianoCerri,LilianCataldiRodrigues,
MariseLopesFermino,MarceloPapoti,RichardD.Cummings,
SeanR.Stowell,andMarceloDias-Baruffi
17 ExploringtheGalectinNetworkbyLight
andFluorescenceMicroscopy ............................................ 307
GabrielGarcı´aCaballero,JoachimC.Manning,AdeleGabba,
DonellaBeckwith,ForrestG.FitzGerald,TanjaJ.Kutzner,
Anna-KristinLudwig,HerbertKaltner,PaulV.Murphy,
MareCudic,andHans-JoachimGabius
18 MolecularImagingforInVivoTrackingandDetection
ofGalectinBindingPartners ............................................. 339
ThaisCanassaDeLeo,SofiaNascimentodosSantos,
EmersonSoaresBernardes,RichardD.Cummings,
SeanR.Stowell,andMarceloDias-Baruffi
19 VisualizationofCytosolicGalectinAccumulationAround
DamagedVesiclesandOrganelles......................................... 353
Ming-HsiangHong,I-ChunWeng,Fang-YenLi,andFu-TongLiu
20 TranscytosisofGalectin-3inMouseIntestine .............................. 367
AlenaIvashenka,ChristianWunder,ValerieChambon,
EstelleDransart,LudgerJohannes,andMassiullahShafaq-Zadah
21 EvaluatingtheRoleofGalectinsinClathrin-Independent
Endocytosis............................................................ 391
MohitP.Mathew,JulieG.Donaldson,andJohnA.Hanover
22 ExaminationofGalectin-3RecruitmentintoMultivesicularBodies
forExosomalSecretion.................................................. 413
SebastianBa€nfer,SophieKutscher,andRalfJacob
Contents xi
23 ManipulatingGalectinExpressioninZebrafish(Daniorerio)................. 425
ChiguangFeng,MihaiNita-Lazar,NuriaGonza´lez-Montalba´n,
JingyuWang,JustinMancini,ShengWang,ChinnarajanRavindran,
HafizAhmed,andGerardoR.Vasta
24 ExaminingGalectinGeneRegulationbyReporterAssays.................... 445
SebastianSchmidt,HerbertKaltner,andHans-JoachimGabius
25 ExaminingtheImpactofGalectin-9onLatentHIVTranscription............ 463
OpeyemiS.Adeniji,LeilaB.Giron,andMohamedAbdel-Mohsen
26 EvaluationoftheRoleofGalectinsinParasiteImmunity .................... 475
JaclynSwan,DhanasekaranSakthivel,TravisBeddoe,
MichaelStear,DavidPiedrafita,andSarahPreston
27 EvaluationoftheBactericidalActivityofGalectins.......................... 517
NourineA.Kamili,AnuPaul,Shang-ChuenWu,
MarceloDias-Baruffi,RichardD.Cummings,
ConnieM.Arthur,andSeanR.Stowell
28 DetectionofPhosphatidylserineExposureonLeukocytes
FollowingTreatmentwithHumanGalectins............................... 533
SeanR.Stowell,MarceloDias-Baruffi,
RichardD.Cummings,andConnieM.Arthur
29 DetectionofReactiveOxygenSpeciesinHumanNeutrophils
UnderVariousConditionsofExposuretoGalectin ......................... 549
LilianCataldiRodrigues,DanielGiulianoCerri,
CleniM.Marzocchi-Machado,RichardD.Cummings,
SeanR.Stowell,andMarceloDias-Baruffi
30 AnalysisofGalectin-BindingReceptorsonBCells.......................... 565
AsmiChakraborty,NorhanB.B.Mohammed,
AngelaE.Bernasconi,andCharlesJ.Dimitroff
31 MethodsforAssessingtheEffectsofGalectinsonLeukocyte
TraffickingandClearance................................................ 581
HannahL.LawandDianneCooper
32 ExaminationoftheContributionsofMaternal/Placental-Derived
Galectin-1toPregnancyOutcome........................................ 603
SophiaBorowski,NancyFreitag,IrisUrban,
GeertMichel,GabrielaBarrientos,andSandraM.Blois
33 MethodtoStudytheRoleofGalectinsinAngiogenesis
InVivoUsingtheChickChorioallantoicMembraneAssay................... 621
KittyC.M.CastricumandVictorL.J.L.Thijssen
34 UntanglingGalectin-MediatedCircuitsthatControl
Hypoxia-DrivenAngiogenesis............................................ 635
NadiaBannoud,P.AlfredoGarcı´a,JulianGambarte-Tudela,
VictoriaSundblad,Alejandro J.Cagnoni,CamilaA.Bach,
JuanM.Pe´rezSaez,AdaG.Blidner,Sebastia´nM.Maller,
KarinaV.Marin˜o,MarianaSalatino,JuanP.Cerliani,
GabrielA.Rabinovich,andDiegoO.Croci