Full Text - Utrecht University Repository - Universiteit Utrecht

154 Pages·2004·3.59 MB·English

by  A. Kidson

IN VITRO EMBRYO DEVELOPMENT IN THE PIG
impact of oocyte maturation milieu on blastocyst morphology and viability

Annadie
KIDSON
In vitro embryo development in the pig

impact of oocyte maturation milieu on blastocyst morphology and viability

Annadie Kidson

2004
Kidson, Annadie
In vitro embryo development in the pig: impact of oocyte maturation milieu on blastocyst
morphology and viability
2004

Dissertation Utrecht University, Faculty of Veterinary Medicine
- with a summary in Dutch -

ISBN 90-393-3730-6

Printing: DPP-Utrecht, Houten
In vitro embryo development in the pig

impact of oocyte maturation milieu on blastocyst morphology and viability

In vitro embryo-ontwikkeling in het varken:

effect van het milieu van eicelmaturatie op de morfologie en levensvatbaarheid
van de blastocyst

(met een samenvatting in het Nederlands)

Proefschrift

Ter verkrijgen van de graad van doctor aan de Universiteit Utrecht op gezag van de
Rector Magnificus, Prof. Dr. W.H. Gispen, ingevolge het besluit van het College voor
Promoties in het openbaar te verdedigen op donderdag 27 mei 2004 des ochtends te
10.30 uur

door

Annadie Kidson

geboren op 10 April 1971 te Johannesburg, Zuid-Afrika
Promotor: Prof. Ben Colenbrander
Department of Farm Animal Health
Faculty of Veterinary Medicine, Utrecht University

Co-promotoren: Dr. Steph J. Dieleman
Department of Farm Animal Health
Faculty of Veterinary Medicine, Utrecht University

Dr. Mart M. Bevers
Department of Farm Animal Health
Faculty of Veterinary Medicine, Utrecht University

The investigations described in this thesis were financially supported by the Graduate
School for Animal Health, the Department of Farm Animal Health of the Faculty of
Veterinary Medicine of Utrecht University and Grant sponsor EU Commission Grant
number QLK3-CT1999-00104

Printing of this thesis was financially supported by:

Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht
University

Dierenarts coöperatie AUV

Graduate School for Animal Health, Faculty of Veterinary Medicine, Utrecht
University

Institute for Pig Genetics, Beuningen, The Netherlands

Intervet Nederland BV

TABLE OF CONTENTS

Chapter 1 General introduction 1

Chapter 2 The influence of sow or gilt follicular fluid in vitro maturation 15
microenvironment, on fertilization, blastocyst morphology and
apoptosis in prepubertal gilt oocytes

Chapter 3 Effects of sperm dose and cumulus cells on the incidence and 37
pattern of polyspermy in prepubertal gilt oocytes, as modulated by
sow or gilt follicular fluid during in vitro oocytes maturation

Chapter 4 Effect of follicle-stimulating hormone on nuclear and cytoplasmic 53
maturation of sow oocytes in vitro

Chapter 5 The effect of oviductal epithelial cell co-culture during in vitro 65
maturation on sow oocyte morphology, fertilization and embryo
development

Chapter 6 Quality of porcine blastocysts produced in vitro in the presence or 79
absence of growth hormone

Chapter 7 Morphology-related cytoskeleton dynamics in porcine blastocysts 99
produced in vitro or in vivo, and its consequences on embryo
viability

Chapter 8 Summarizing discussion 119

Samenvatting 133
Acknowledgments 139
Curriculum Vitae 143

“Life is full of cactus, but we don’t have to sit on it.”
Unknown

Vir my familie,

Vir Stephan,

En vir Mart,

Deo Gloria!