Table Of ContentFluorescence Imaging and
Biological Quantification
Fluorescence Imaging and
Biological Quantification
Edited by
Raquel Seruca
Jasjit S. Suri
João M. Sanches
CRC Press
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Library of Congress Cataloging‑in‑Publication Data
Names: Seruca, Raquel, editor. | Suri, Jasjit S., editor. | Sanches,
João M., editor.
Title: Fluorescence imaging and biological quantification / [edited by]
Raquel Seruca, Jasjit S. Suri, and João M. Sanches.
Description: Boca Raton : Taylor & Francis, 2017. | Includes bibliographical
references.
Identifiers: LCCN 2017013420 | ISBN 9781498737043 (hardback : alk. paper)
Subjects: | MESH: Optical Imaging | Cytological Techniques | Molecular
Imaging | Image Interpretation, Computer-Assisted
Classification: LCC R857.O6 | NLM WN 195 | DDC 616.07/54--dc23
LC record available at https://lccn.loc.gov/2017013420
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Contents
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . .xiii
Editors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxvii
Section i ADVAnceD MetHoDS oF AnALYSiS
1 Confocal microscopy in the life sciences . . . . . .3
Miguel Aroso and M. Gomez-Lazaro
2 Imaging flow cytometry for quantification of
cellular parameters . . . . . . . . . . . . . . . . . . . .25
Catarina R. Almeida
3 Live-cell imaging: Seeing is believing . . . . . . .39
Patrícia Castro and Patrícia Carneiro
4 Atomic force microscopy: A tool for imaging,
manipulating biomolecules, and quantifying
single-molecule interactions in biological
systems . . . . . . . . . . . . . . . . . . . . . . . . . . .49
Catarina S. Lopes, Filomena A. Carvalho,
and Nuno C. Santos
vii
Contents
5 Confocal Raman microscopy: Imaging the
chemistry . . . . . . . . . . . . . . . . . . . . . . . . . .65
M. Gomez-Lazaro, A. Freitas, and C.C. Ribeiro
6 Tracking cancer: In vivo imaging techniques . . . 85
Patrícia Carvalho, André Roma, and Sérgia Velho
7 Quantum dots: Concepts, imaging, and
therapeutic applications in cancer . . . . . . . . 105
Maria Sofia Fernandes, Anabela Ferro,
Patrícia Carneiro, Raquel Seruca,
and João M. Sanches
8 Selecting the appropriate in situ proximity
ligation assay protocol . . . . . . . . . . . . . . . . 119
Doroteya Raykova, Linda Arngården,
Axel Klaesson, Johan Heldin, and Ola Söderberg
9 Cell detection and joint shape tracking using
local image filters . . . . . . . . . . . . . . . . . . . . 133
Tiago Esteves and Pedro Quelhas
Section ii MoLecULAR AnD
ceLLULAR APPLicAtionS
10 Studying subcellular signaling events
in living microglial cells using
fluorescence resonance energy
transfer-based nanosensors . . . . . . . . . . . . .165
Renato Socodato, Camila C. Portugal,
Paula Sampaio, and João B. Relvas
11 Membrane trafficking under the microscope,
what new imaging technologies have brought
to light . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Julia Fernandez-Rodriguez and Tommy Nilsson
viii
Contents
12 Illuminating the cycle of life . . . . . . . . . . . . 201
Anabela Ferro, Patrícia Carneiro,
Maria Sofia Fernandes, Tânia Mestre,
Ivan Sahumbaiev, João M. Sanches,
and Raquel Seruca
13 Methods for the visualization of circadian
rhythms: From molecules to organisms . . . . . 231
Rukeia El-Athman, Jeannine Mazuch, Luise Fuhr,
Mónica Abreu, Nikolai Genov, and Angela Relógio
14 Measurement of lysosomal ion homeostasis
by fluorescence microscopy . . . . . . . . . . . . . 259
Benjamin König, Lisa von Kleist, and Tobias Stauber
15 Capturing quantitative features of protein
expression from in situ fluorescence microscopic
images of cancer cell populations . . . . . . . . . . . 279
Joana Figueiredo, Ana Sofia Ribeiro,
Tânia Mestre, Sofia Esménio, Martina Fonseca,
Joana Paredes, Raquel Seruca, and João M. Sanches
16 Cancer cell invadopodia: Visualization and
quantification tools . . . . . . . . . . . . . . . . . . .299
Angela Margarida Costa and Maria José Oliveira
index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .317
ix
