FluidFM technology

26 Pages·2013·5.28 MB·English

by  Tomaso Zambelli

FluidFM technology:
from single-cell manipulation to nanoparticle lithography
Tomaso Zambelli
Laboratory of Biosensors and Bioelectronics
Institute for Biomedical Engineering D-ITET
www.lbb.ethz.ch
1
force-controlled patch clamp?
PhD M. Gabi (Oct 2006)
inspiration
Laboratory of Biosensors and
Bioelectronics → patch-clamp technique*
→ only optical control → hit-and-miss
objective
development of a microinjector with force feedback screening cascade
to be operated in liquid environment (GABA receptors)
challenge
combining nanofluidics and atomic force microscopy (AFM)
* E. Neher and B. Sakmann, 1991 Nobel Laureates in Medicine
Neher et al, Nature 260 (1976) 799 Hamill et al, Pflügers Arch 391 (1981) 85 2
hollow AFM cantilevers
Nanoscale dispensing of liquids
Fountain pen nanochemistry:
Atomic force control of chrome etching through cantilevered probes
Meister et al, Microel Eng 67–68 (2003) 644 → NADIS
Lewis et al, Appl Phys Lett 75 (1999) 2689
Meister et al, Appl Phys Lett 85 (2004) 6260
Meister et al, NSTI-Nanotech 2008 v3, p273
[Hug et al, Dig Tech Pap Transducers ′05 2 (2005) 1191]
in air!
Neuchâtel (CH)
capillarity
Micromachined fountain pen for
AFM-based nanopatterning
A Nanofountain Probe with Sub-100 nm
Deladi et al, Appl Phys Lett 85 (2004) 5361 Molecular Writing Resolution
Loh et al, Small 1 (2005) 632
volcano tips
Loh et al, Small 5 (2009) 1667
with cells but removing the buffer
3
FluidFM: concept
PhDs M. Gabi, P. Behr
JPK AFM Nanowizard I together with
Neuchâtel (CH)
buffer
glass slide
Nano Lett 9 (2009) 2501 4
micro- nanoinjectors
nanotube endoscope
glass micropipette
Singhal et al,
Barber,
Nat Nanotech 6 (2011) 57
J Kansas Med Sot 4 (1904) 489
FluidFM
pyramidal tip
Nano Lett 9 (2009) 2501
1 µm
5
perforation vs. gentle contact
4
*
perforation

)
N
n
(2

e
c
r gentle contact
o
F
0
*
200 nm
FIB → J. Bitterli
200 nm
(PhD, CSEM)
Nano Lett 9 (2009) 2501
6
spatial manipulation
PhDs P. Dörig, P. Stiefel
z z
Prof. Vorholt (IMB ETH D-BIOL)
budding yeasts
1 2
x
z
y
3 4
[pN, µN]
spread neurons
30 µm
Appl Phys Lett 97 (2010) 023701
7
FluidFM
Commercialization
Research
www.cytosurge.com
(Dr. M.Gabi and P.Behr)
Electrophysiology Biology
Biosensing Engineering
coll. Prof. H.Abriel coll. Prof. J.A.Vorholt
Lithography Skeleton
SICM Infection
coll. Dr. J.Mercer
Chemistry SU-8
Patch clamp
Adhesion
Electrochemistry
Self-Sensing
Ion mapping (ISE) coll. Dr. Polesel Maris
Injection
Nano-optics
Neuron stimulation (MEA) Omics analysis MultiProbe
(extraction)
 beyond the proof-of-concept!
 try to answer or stimulate an original question…
8
FluidFM probes
Dr. Edin Sarajlic
SmartTip & Cytosurge
Deladi et al, Appl Phys Lett 85 (2004) 5361
Berenschot et al, Small 8 (2012) 3823
down to 50 nm
down to 2 µm
10 µm
9
the wrath
Μῆνιν ἄειδε θεὰ Πηληϊάδεω Ἀχιλῆος
οὐλομένην… (Homer, Iliad I:1-2)
AFM probes
 F(z)  k
 stability  aperture
 electrodes  Au layer
results
complementary
scientific
instrumentation
questions
 pressure controller
 optical microscope
 patch clamp electronics
 …
10