Table Of ContentEsterases, Lipases, and
Phospholipases
From Structure to Clinical Significance
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Series A: Life Sciences
Esterases, Lipases, and
Phospholipases
From Structure to Clinical Significance
Edited by
M. I. Mackness
University of Manchester
Manchester, United Kingdom
and
M. Clerc
University of Bordeaux II
Bordeaux, France
Springer Science+Business Media, LLC
Proceedings 01 a NATO Advanced Research Workshop on
Esterases, Lipases, and Phospholipases,
held September 22-24, 1993,
in Bordeaux, France
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Llbrary of Congress Cataloging-ln-Publication Data
Esterases. l1pases. and phasphol1pases from structure ta cllnlcal
slgnlfleanee I edlted by M.I. Mackness and M. Clerc.
p. em. -- (NATO ASI serles. Series A, Llfe selenees ; v.
266)
"Proeeedlngs of a NATO Advaneed Researeh Workshop on Esterases,
Lipases, and Phosphol ipases, held Septeober 22-24, 1993. in
Bordeaux. Franee"--T.p. verso.
"Published in eooperation with NATO Selentific Affalrs Divlsion."
Includes b1bliographical references and index.
ISBN 978-1-4899-0995-4 ISBN 978-1-4899-0993-0 (eBook)
DOI 10.1007/978-1- 4899-0993-0
1. Esterases--Congresses. 2. Llpase--Congresses.
3. Phosphol ipases--Congresses. 1. Maekness, M. 1. (Mlchael 1.)
II. Clerc. M. (Mlc~el) III_ NATO Advanced Research Warkshop on
Esterases, Lipases. and Phospholipases (1993 Bordeaux. France)
IV. Series.
[DNLM: 1. Esterases--congresses. 2. L 1p ase--congresses.
3. Phospholipases--congresses. OU 136 E79 1994l
OP609.E8EBB 1994
612' .0151--dc20
DNLM/DLC
for Library of Congress 94-36095
CIP
ISBN 978-1-4899-0995-4
©1994 Springer Science+Business Media New York
Originally published by Plenum Press, New York in 1994
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PREFACE
The NATO-Advanced Research Workshop "Esterases, Lipases
and Phospholipases: From Structure to Clinical Significance"
was held at the University of Bordeaux II, France from 22nd-
24th September 1993 under the Directorship of Professor
Michel Clerc of the University of Bordeaux II. The meeting
was organised by Hugues Chap (INSERM U 326, Toulouse,
France), Georges Ferard (University of Strasbourg, France),
Wolfgang Junge (University of Kiel, Germany) and Michael
Mackness (University of Manchester, UK).
In recent years it has become increasingly apparent
that hydrolytic enzymes of the esterase, lipase and
phospholipase type play central roles in the pathophysiology
of many human diseases. The purpose of this NATO-ARW was to
bring together experts (both clinical and scientific) in all
three interrelated fields to review the current basic and
clinical position and discuss future developments
particularly with respect to future research aimed at
determining the basic biochemical lesion involving
hydrolytic enzymes involved in human disease and the use of
these enzymes in diagnosis.
As well as formal lectures from established
researchers, the meeting also involved a number of lively
round-table discussions on future developments and
presentations from younger research workers, all of which
are recorded in this Proceedings and which contribute to the
success of the meeting.
As well as the substantial financial assistance which
was received from NATO, the Organising Committee would also
like to express their appreciation to the following, without
whose assistance (financial and otherwise) the meeting would
not have been as successful:-
INSERM, Society of Biochemistry and Molecular Biology
(France), Society of Clinical Biology (France), Association
of Clinical Biochemists (UK), Abbot, Baxter, Beaufour,
Behring, Bioxytech, Boehringer-Mannheim, corata, French
Foreign Office, Fournier-Dijon, Helena France, Iris-Servier,
Jonan, Kodak, Lipha Oberval, Pierre Fabre, Radiometer,
Sanofi, Sebia, and the Regional and local councils and
University of Bordeaux II and the Hospitals of Bordeaux.
v
CONTENTS
overview - Lipases and Esterases: Reassessment of their
Classification in the Light of their Three
Dimensional Structure............................. 1
R. Verger, F. Ferrato, M. Aoubala, A. de Caro, M.
Ivanova, L. de la Fourniere, F. Carriere, c.
Cudrey, c. Riviere, N. Rugani, Y. Gargouri, A.
Hjorth, H. Woldike, E. Boel, L. Thim. D.M.
Lawson, G.G. Dodson, H. van Tilbeurgh, M.P.
Egloff and c. Cambillau
ESTERASES
Serum Cholinesterase: Genetics, Enzymology, Diagnostic
Use and the Association with Clinical Disorders •• 13
E. Schmidt and F.W. Schmidt
Serum Cholinesterase in Diabetes 27
K.M. Kutty
Lipid Feeding and Serum Esterases ..........•........... 35
H.A. Van Lith, B.F.M. Van Zutphen and A.C. Beynen
Liver Carboxylesterases: Topogenesis of Intracellular
and secreted Forms . . . . . . • . • . . . . . . . • . . . . . . . . . . . . . . 4 7 ·
M. Robbi and H. Beaufay
Catalytic Properties and Classification of Phosphoric
Triester Hydrolases: EDTA-Insensitive and EDTA
Sensitive Paraoxonases in Sera of Non-Diseased
and Diseased Population Groups................... 57
E. Reiner and v. Simeon
The Human Serum Paraoxonase Polymorphism and
Atherosclerosis . . . . . . . . . . . . . . . . . . . . . • . . . . . • . • . . . . 65
M.I. Mackness
Hydrolysis of Xenobiotic Fatty Acid Esters by
Carboxylesterases of Human Skin.................. 75
E. Heymann, F. Noetzel, R. Retzlaff, G. Schnetgoke
and s. Westie
vii
Human Monocyte/Macrophage Serine Esterase-1: Expression
in Haematopoiesis and Transcript Deficiency in
Alveolar Macrophages Derived from Patients
Affected by Pulmonary Fibrosis................... 85
F. Zschunke, J. Barth, H. Kreipe and H.J. Radzun
Interactions between Pesticides and Esterases in
Humans . . . . . . . . . . . . . . . . • • . . . . . . . . . . . . . • . . . . . . . . . . . 91
C.H. Walker
The Role of Esterases in the Pathogenesis of Disease
and their use as Diagnostic Tools .....•....•..... 99
W. Junge
Cross-Reaction of a Human Anti-Paraoxonase Monoclonal
Antibody with a Rat High Density Lipoprotein
Peptide . • . . . • . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . • . . . . 103
M-C. Blatter Garin, s. Messmer, D. Pometa and R.W.
James
Carboxyl Ester Lipase in Acinar Cell Carcinoma of
Pancreas • . . . • . . . . • • . . . . . . . . . . . . . . . . . . . . . . . . . • . . . . 107
M.N. Reddy
BIOCHEMISTRY OF LIPASES AND RELATED LIPOLYTIC ENZYMES
Hepatic Triglyceride Lipase and Lipoprotein Lipase
action in vitro and in vivo ...................... 115
L. Chan, J. Wolle, J-Y. Lo, E. Zsigmond and L.C.
Smith
Lipoprotein Lipase and Lysosomal Acid Lipase: Two
Key Enzymes of Lipid Metabolism ..•..........••••• 121
D. Ameis and H. Greten
Lipoprotein Lipase Activity in the Pathological
Metabolism of Lipoproteins ....................... 129
P.N. Durrington
Gastric Lipase: Characteristics and Biological Function
of Preduodenal Digestive Lipases ................. 139
M. Hamosh and P. Hamosh
Pancreatic Cholesteryl Esterase in Health and Disease .. 149
E. Mas and D. Lombardo
Pancreatic Lipase, Colipase and Enterostatin - A
Lipolytic Triad • . . . . . . • . . . . . . . . . . . . . . . . . . . . . • . . . 159
c. Erlanson-Albertsson
LCAT: From Structure to Clinical Significance 169
M.F. Dumon, A. Berard and M. Clerc
Development of a Specific Assay for Pancreatic Lipase
Activity for Diagnostic Purposes •.....•.......... 179
G. Ferard, J.M. Lessinger, P. Arzoglou, A. Visvikis
and W. Junge
viii
Post-heparin Lipolytic Activities in Chronic Renal
Failure Patients in Relation to Plasma
Triglycerides • . • . . . • . . . . . . . . • . . . . . . . . . . . . . . . . . . . . 183
A. Abdallah, M.P. de Zulueta, P. Richard, D.
Higueret, A. Cassaigne and A. Iron
Rabbit Pancreatic Lipase: Purification and Some
Novel Catalytic Properties •.•••.••..•••••••.•••.. 187
A. Lykidis, A. Avranas, v. Mougios and P. Arzoglou
PHOSPHOLIPASES A2
Phospholipase A2 and Prostaglandin Formation in Rat
Glomerular Mesangial Cells...................... 193
H. Van den Bosch, M.J.B.M. Vervoordeldonk, R.M.
Sanchez, J. Pfeilschifter and e.G. Schalkwijk
Phospholipase A2 and the Pathogenesis of Multisystem
Organ Failure in Experimental and Clinical
Endotoxin Shock • . . . • . • . . . . . . . . . . . . . . • • . . • • . . • • . . 203
P. Vadas, J. Edelson and w. Pruzanski
Transcriptional Regulation of Secreted Phospholipase A2
in Human HepG2 Cells, Rat Astrocytes and Rabbit
Articular Chondrocytes ..•......................• 213
M. Ziari, F. Berenbaum, Q. Fan, c. Salvat, M.T.,
Corvol, J.L. Olivier and G. Bereziat
Phospholipase A2 in Intensive-Care Patients: The German
Multicentre Trial • . • . . . . . . . . . . . . • . . . . . . . . . . . . . . . 223
W.H. Uhl and M.W. Buchler
PHOSPHOLIPASES
Study of Some Mechanisms that May Contribute to the
Presence of High Levels of Phospholipase A2
Activity in Plasma of Patients with Septicemia 235
M.A. Gij6n, c. Garcia, c. Perez, F. Lopez-Diez and
M. Sanchez-Craspo
Phospholipase Action in the Generation of Modified
LDL . . . • . . . . . . . . . . . . . . • . . . . . . . . . . . . . . . . . . . . . . . . . . 245
s. Parthasarathy and J. Barnett
Detection of Phospholipases A2 in Serum in Acute
Pancreatitis . . . • . . . • . • . . . . . • . . . . . . . . . . . . . . . . • . . . 253
T.J. Nevalainen and J.M. Gronroos
Methods for the Determination of the Activity of
Phospholipases and Their Clinical Application 263
J. Aufenanger, M. samman and M. Quintel
Phospholipases as Clinical Tools: Measurement 273
H. Chap
ix
Contributors 275
Index •.....•....................•...•..•....•.•.•...•.• 277
X
