Table Of ContentEpithelial Transport
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Epithelial Transport
A guide to methods and
experimental analysis
Edited by
Nancy K. Wills, Luis Reuss
and Simon A. Lewis
Department of Physiology and Biophysics,
University of Texas Medical Branch, Galveston, Texas, USA
CHAPMAN &. HAll
London· Weinheim . New York· Tokyo· Melbourne· Madras
Published by Chapman & Hall, 2-6 Boundary Row, London SEl 8HN, UK
Chapman & Hall, 2-6 Boundary Row, London SEI8HN, UK
Chapman & Hall GmbH, Pappelallee 3, 69469 Wemhelm, Germany
Chapman & Hall USA, 115 Fifth Avenue, New York, NY 10003, USA
Chapman & Hall japan, lTP-japan, Kyowa BUlldmg, 3F, 2-2-1 Hirakawacho,
Chlyoda-ku, Tokyo 102, japan
Chapman & Hall Austraha, 102 Dodds Street, South Melbourne,
Victona 3205, Austraha
Chapman & Hall India, R. Seshadri, 32 Second Mam Road, CIT East,
Madras 600 035, India
Fust edition 1996
© 1996 Chapman & Hall
Softcover reprint of the hardcover 1st edition 1996
Typeset m 1O/12pt Palatmo by AFS Image Setters Ltd, Glasgow
ISBN-13: 978-94-010-7168-0 e-ISBN-13: 978-94-009-1495-7
001:10.1007/978-94-009-1495-7
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Contents
List of contributors Vll
Preface xi
1 Epithelial structure and function 1
Simon A. Lewis
2 Epithelial transport proteins 21
Luis Reuss, Nancy K. Wills and Simon A. Lewis
3 Epithelial polarity 49
M. Cereijido, R.G. Contreras, M.R. Garcia-Villegas,
L. Gonzales-Mariscal and J. Valdes
4 Characterization of epithelial ion transport 70
Calvin U. Cotton and Luis Reuss
5 Epithelial electrophysiology 93
Simon A. Lewis
6 Impedance analysis of epithelia 118
Simon A. Lewis, Chris Clausen and Nancy K. Wills
7 Measurements and interpretation of cytoplasmic
ion activities 146
Guillermo A. Altenberg and Luis Reuss
8 Measurements of epithelial cell volume 167
Luis Reuss, Calvin U. Cotton and Guillermo A. Altenberg
VI Contents
9 Methods and experimental analysis of isolated
epithelial cell membranes 190
Austin K. Mircheff
10 Methods and experimental analysis of single ion channels 212
Simon A. Lewis
11 Epithelial cell culture 236
Nancy K. Wills
12 Signaling pathways regulating ion transport in polarized cells 256
Norman J. Karin, Min LN. Zhang, E. Radford Decker
and Roger O'Neil
13 The cytoskeleton and epithelial function 275
John W. Mills
14 Future perspectives: molecular biology and pathophysiology 306
Alicia McDonough
Appendix A: Instrumentation 323
Appendix B: Solving simple equivalent circuits 325
Appendix C: A spread-sheet for marker distribution data 329
Index 335
Contributors
Guillermo A. Altenberg MD
Department of Physiology and Biophysics
University of Texas Medical Branch
Galveston
Texas 77555-0641
USA
M. Cereijido MD, PhD
Centro de Investigation y de Studios Avanzados
Fuselage, Biofisica y Neurociencias
Apartheid Postal 14-740
07000 Mexico DF
Mexico
Chris Clausen PhD
Department of Physiology and Biophysics
State University of New York at Stony Brook
Stony Brook
New York 11704-8861
USA
R. G. Contreras PhD
Centro de Investigacion y de Estudios A vanzados
Fisologia, Biofisica y Neurociencias
Apartado Postal 14-740
07000 Mexico DF
Mexico
Calvin U. Cotton PhD
Department of Physiology
Case Western Reserve
Cleveland
Ohio 44106-4948
USA
viii Contributors
E. Radford Decker PhD
Department of Pharmacology
Texas Biotechnology Corporation
Houston 77030
Texas
USA
M. R. Garda-Villegas MD
Centro de Investigacion y de Estudios A vanzados
Fisologia, Biofisica y Neurociencias
Apartado Postal 14-740
07000 Mexico DF
Mexico
L. Gonzalez-Mariscal MD
Centro de Investigacion y de Estudios Avanzados
Fisologia, Biofisica y Neurociencias
Apartado Postal 14-740
07000 Mexico DF
Mexico
Norman J. Karin PhD
Department of Integrative Biology
University of Texas, Houston Health Science Center
Houston
Texas 77030
USA
Simon A. Lewis PhD
Department of Physiology and Biophysics
University of Texas Medical Branch
Galveston
Texas 77555-0641
USA
Alicia McDonough PhD
Department of Physiology and Biophysics
University of Southern California, School of Medicine
1333 San Pablo
Los Angeles
CA 90033-1026
USA
Contributors IX
John W. Mills PhD
Department of Biology
Clarkson University
Potsdam
NY 13676
USA
Austin K. Mircheff PhD
Department of Physiology and Biophysics
University of Southern California, School of Medicine
1333 San Pablo
Los Angeles
CA 90033-1026
USA
Roger O'Neil PhD
Department of Integrative Biology
University of Texas, Houston Health Science Center
Houston
Texas 77030
USA
Luis Reuss MD
Department of Physiology and Biophysics
University of Texas Medical Branch
Galveston
Texas 77555-0641
USA
J. Valdes PhD
Centro de Investigacion y de Estudios A vanzados
Fisologia, Biofisica y Neurociencias
Apartado Postal 14-740
07000 Mexico DF
Mexico
Nancy K. Wills PhD
Department of Physiology and Biophysics
University of Texas Medical Branch
Galveston
Texas 77555-0641
USA
x Contributors
Min LN. Zhang MD
Department of Integrative Biology
University of Texas, Houston Health Science Center
Houston
Texas 77030
USA
