Table Of ContentCURRENT TOPICS IN
Cellular Regulation
Volume 24 — 1984
Enzyme Catalysis
and Control
Editors
Marlene DeLuca · Henry Lardy
Department of Chemistry Institute for Enzyme Research
University of California, San Diego University of Wisconsin
La Jolla, California Madison, Wisconsin
Richard L. Cross
Department of Biochemistry
Upstate Medical Center
State University of New York at Syracuse
Syracuse, New York
ACADEMIC PRESS, INC.
(Harcourt Brace Jovanovich, Publishers)
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C O P Y R I G H T © 1984 , BY A C A D E M I C P R E S S , I N C .
ALL RIGHTS RESERVED.
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PRINTED IN THE UNITED STATES OF AMERICA
84 85 86 87 9 8 7 6 5 4 3 2 1
To Paul, with admiration, respect, love, and thanks,
from the past and present members of your laboratory group
Contributors
Numbers in parentheses indicate the pages on which the authors' contributions begin.
CLAUDE AFLALO (435), Department of Biology, Ben-Gurion University of
the Negev, 84105 Beer-Sheva, Israel
ANTOINE AMORY (471), Laboratoire d'Enzymologie, Université de Lou-
vain, 1348 Louvain-la-Neuve, Belgium
SEELOCHAN BEHARRY (365), Department of Chemistry, Simon Fraser Uni-
versity, Burnaby, British Columbia V5A 1S6, Canada
IRAJ BEHBAHANI-NEJAD (219), Department of Chemistry, Michigan State
University, East Lansing, Michigan 48824
ROBERT W. BERNLOHR (145), Department of Biochemistry, Microbiology,
Molecular and Cell Biology, The Pennsylvania State University, Uni-
versity Park, Pennsylvania 16802
L. L. BIEBER (111), Department of Biochemistry, Michigan State Univer-
sity, East Lansing, Michigan 48824
PAUL BOYER (471), Department of Chemistry and Molecular Biology Insti-
tute, University of California, Los Angeles, Los Angeles, California
90024
WILLIAM A. BRIDGER (345), Department of Biochemistry, University of
Alberta, Edmonton, Alberta T6G 2H7, Canada
D. CARROLL (21), Department of Biochemistry, University of Massachu-
setts, Amherst, Massachusetts 01003
PHILIPPE CHAMPEIL (397), Service de Biophysique, Centre d'Etudes Nu-
cléaires de Saclay, 91191 Gif sur Yvette, France
STEPHEN G. CHANEY (251), Department of Biochemistry, University of
North Carolina School of Medicine, Chapel Hill, North Carolina
27514
1
YEN CHUNG CHANG (181), Department of Biochemistry and Biophysics,
Iowa State University, Ames, Iowa 50011
MILDRED COHN (1), Institute for Cancer Research, Fox Chase Cancer
Center, Philadelphia, Pennsylvania 19111
1
Present address: Department of Biochemistry, University of Illinois Medical School,
Chicago, Illinois 60612.
xi
Xll CONTRIBUTORS
R. TOBIAS CONSIDINE (251), Department of Biochemistry, University of
North Carolina School of Medicine, Chapel Hill, North Carolina
27514
RICHARD L. CROSS (335), Department of Biochemistry, State University of
New York Upstate Medical Center, Syracuse, New York 13210
DAVID CUNNINGHAM (335), Department of Biochemistry, State University
of New York Upstate Medical Center, Syracuse, New York 13210
A. STEPHEN DAHMS (447), Department of Chemistry and Molecular Biology
Institute, San Diego State University, San Diego, California 92182
MARLENE DELUCA (189), Department of Chemistry, University of Califor-
nia, San Diego, La Jolla, California 92093
LEOPOLDO DEMEIS (15), Departamento de Bioqutmica, Instituto de Ciên-
cias Biomédicas, Universidade Federal do Rio de Janeiro, Cidade
Universitaria, Rio de Janeiro, RJ Brazil
MARY E. DEMPSEY (63), Department of Biochemistry, University of Minne-
sota Medical School, Minneapolis, Minnesota 55455
ROBERT B. DENMAN (153), Biochemistry Division, College of Science, The
Pennsylvania State University, University Park, Pennsylvania 16802
2
TIMOTHY J. DONOHUE (145), Department of Biochemistry, Microbiology,
Molecular and Cell Biology, The Pennsylvania State University, Uni-
versity Park, Pennsylvania 16802
JAMES L. DYE (219), Department of Chemistry, Michigan State University,
East Lansing, Michigan 48824
K. E. EBNER (51), Department of Biochemistry, University of Kansas Medi-
cal Center, Kansas City, Kansas 66103
GAYLE R. ERDHEIM (301), Department of Chemistry, University of Califor-
nia, Berkeley, Berkeley, California 94720
LARS ERNSTER (313), Department of Biochemistry, Arrhenius Laboratory,
University of Stockholm, S-106 91 Stockholm, Sweden
C. J. FIOL (111), Department of Biochemistry, Michigan State University,
East Lansing, Michigan 48824
HERBERT J. FROMM (197), Department of Biochemistry and Biophysics,
Iowa State University, Ames, Iowa 50011
3
ARI GAFNI (273), Department of Chemical Physics, The Weizmann Insti-
tute of Science, Rehovot 76100, Israel
4
J. E. GANDER (99), Department of Biochemistry, College of Biological
Sciences, University of Minnesota, St. Paul, Minnesota 55108
2
Present address: Department of Microbiology, University of Illinois, Urbana, Illinois
61801.
3
Present address: Institute of Gerontology and Department of Biological Chemistry,
The University of Michigan, Ann Arbor, Michigan 48109.
4
Present address: Department of Microbiology and Cell Science, Institute of Food and
Agricultural Sciences, University of Florida, Gainesville, Florida 32611.
CONTRIBUTORS xiii
NANCY J. GANSON (197), Department of Biochemistry and Biophysics, Iowa
State University, Ames, Iowa 50011
J. GIKNER (21), Department of Biochemistry, University of Massachusetts,
Amherst, Massachusetts 01003
MARCEL-PIERRE GINGOLD (397), Service de Biophysique, Centre d'Etudes
Nucléaires de Saclay, 91191 Gif sur Yvette, France
ANDRÉ GOFFEAU (471), Laboratoire d'Enzymologie, Université de Lou-
vain, 1348 Louvain-la-Neuve, Belgium
KENNETH GOLDMAN (301), Department of Chemistry, University of Cali-
fornia, Berkeley, Berkeley, California 94720
YALEE. GOLDMAN (357), Department of Physiology, University of Pennsyl-
vania School of Medicine, Philadelphia, Pennsylvania 19104
DONALD J. GRAVES (181), Department of Biochemistry and Biophysics,
Iowa State University, Ames, Iowa 50011
MICHAEL J. GRESSER (365), Department of Chemistry, Simon Fraser Uni-
versity, Burnaby, British Columbia V5A 1S6, Canada
FLORENT GUILLAIN (397), Service de Biophysique, Centre d'Etudes Nu-
cléaires de Saclay, 91191 Gif sur Yvette, France
DAVID D. HACKNEY (379), Department of Biological Sciences, Carnegie-
Mellon University, Pittsburgh, Pennsylvania 15213
IRIS H. HALL (251), Department of Medicinal Chemistry, University of
North Carolina School of Pharmacy, Chapel Hill, North Carolina
27514
MARK G. HIBBERD (357), Department of Physiology, University of Pennsyl-
vania School of Medicine, Philadelphia, Pennsylvania 19104
PETER E. HUGHES (171), Institute for Enzyme Research and Department of
Biochemistry, University of Wisconsin, Madison, Wisconsin 53705
DONALD E. HULTQUIST (287), Department of Biological Chemistry, The
University of Michigan, Ann Arbor, Michigan 48109
5
MICHAEL O. HURST (181), Department of Biochemistry and Biophysics,
Iowa State University, Ames, Iowa 50011
6
STEVE JANOVEC (99), Department of Biochemistry, College of Biological
Sciences, University of Minnesota, St. Paul, Minnesota 55108
7
DAVID A. JUCKETT (287), Department of Biological Chemistry, The Uni-
versity of Michigan, Ann Arbor, Michigan 48109
TOHRU KANAZAWA (423), Department of Biochemistry, Asahikawa Medical
College, Asahikawa 078-11, Japan
5
Present address: Department of Chemistry, University of Colorado, Boulder, Colorado
80309.
6
Present address: Plant Biology Program, Department of Biology, Washington Univer-
sity, St. Louis, Missouri 63130.
7
Present address: Department of Biochemistry, St. Jude Children's Research Hospital,
Memphis, Tennessee 38101.
XIV CONTRIBUTORS
JAW-JOU KANG (447), Department of Chemistry and Molecular Biology
Institute, San Diego State University, San Diego, California 92182
CELIK KAYALAR (301), Department of Chemistry, University of California,
Berkeley, Berkeley, California 94720
P. A. KIENER (21), Pharmaceutical Research and Development, Bristol
Meyers Company, 315 Thompson Road N., Syracuse, New York 13206
WILLIAM L. KLEIN (129), Department of Neurobiology and Physiology,
Northwestern University, Evanston, Illinois 60201
GÜNTHER KREIL (261), Institute of Molecular Biology, Austrian Academy of
Sciences, A-5020 Salzburg, Austria
JEAN-JACQUES LACAPERE (397), Service de Biophysique, Centre d'Etudes
Nucléaires de Saclay, 91191 Gif sur Yvette, France
HENRY LARDY (171), Institute for Enzyme Research and Department of
Biochemistry, University of Wisconsin, Madison, Wisconsin 53705
KUO-HSIUNG LEE (251), Department ofMedicinal Chemistry, University of
North Carolina School of Pharmacy, Chapel Hill, North Carolina
27514
DAVID B. MCINTOSH (409, 471), MRC Biomembrane Research Unit/De-
partment of Chemical Pathology, University of Cape Town Medical
School, Cape Town 7925, South Africa
JOËLLE E. MIARA (447), Department of Chemistry and Molecular Biology
Institute, San Diego State University, San Diego, California 92182
ROBERT A. MITCHELL (387), Department of Biochemistry, Wayne State
University, Detroit, Michigan 48202
DENISE M. C. MOENNICH (365), Department of Chemistry, Simon Fraser
University, Burnaby, British Columbia V5A 1S6, Canada
GERALD G. NESLUND (447), Department of Chemistry and Molecular Biol-
ogy Institute, San Diego State University, San Diego, California 92182
ANTHONY W. NORMAN (35), Department of Biochemistry, University of
California, Riverside, Riverside, California 92521
8
LUCY JEAN SANNES (287), Department of Biological Chemistry, The Uni-
versity of Michigan, Ann Arbor, Michigan 48109
9
HAROLD J. SCHREIER (145), Department of Biochemistry, Microbiology,
Molecular and Cell Biology, The Pennsylvania State University, Uni-
versity Park, Pennsylvania 16802
HAROLD L. SEGAL (229), Division of Cell and Molecular Biology, State
University of New York at Buffalo, Buffalo, New York 14260
8
Present address: Abbott Laboratories, 14th Street and Sheridan Road, North Chicago,
Illinois 60064.
9
Present address: Department of Molecular Biology and Microbiology, Tufts University
Medical School, Boston, Massachusetts 02111.
CONTRIBUTORS XV
ARMEN SHANAFELT (301), Department of Chemistry, University of Califor-
nia, Berkeley, Berkeley, California 94720
NOUN SHAVIT (435), Department of Biology, Ben-Gurion University of the
Negev, 84105 Beer-Sheva, Israel
EMANUEL SILVERSTEIN (209), Laboratory of Molecular Biology, Depart-
ments of Medicine and Biochemistry, Downstate Medical Center,
State University of New York at Brooklyn, Brooklyn, New York 11203
GOPALAN SOMAN (181), Department of Biochemistry and Biophysics, Iowa
State University, Ames, Iowa 50011
CLARENCE H. SUELTER (219), Department of Biochemistry, Michigan State
University, East Lansing, Michigan 48824
YUICHI TAKAKUWA (423), Department of Biochemistry, Asahikawa Medical
College, Asahikawa 078-11, Japan
K. TAKASE (51), Department of Biochemistry, University of Kansas Medical
Center, Kansas City, Kansas 66103
JAMES K. TAMURA (335), Department of Biochemistry, State University of
New York Upstate Medical Center, Syracuse, New York 13210
AL L. TAPPEL (87), Department of Food Science and Technology, Univer-
sity of California, Davis, Davis, California 95616
DAVID R. TRENTHAM (357), Department of Biochemistry and Biophysics,
University of Pennsylvania School of Medicine, Philadelphia, Pennsyl-
vania 19104
FREDERICK C. WEDLER (153), Biochemistry Division, College of Science,
The Pennsylvania State University, University Park, Pennsylvania
16802
E. W. WESTHEAD (21), Department of Biochemistry, University of Massa-
chusetts, Amherst, Massachusetts 01003
W. L. WILLIAMS, JR. (251), Department of Medicinal Chemistry, Univer-
sity of North Carolina School of Pharmacy, Chapel Hill, North Caro-
lina 27514
WALKER WILLINGHAM, III (251), Department of Biochemistry, University
of North Carolina School of Medicine, Chapel Hill, North Carolina
27514
JAMES R. WINKLER (229), Division of Cell and Molecular Biology, State
University of New York at Buffalo, Buffalo, New York 14260
JANG-YEN W U (119), Department of Physiology, The Pennsylvania State
University College of Medicine, Hershey, Pennsylvania 17033
Introduction
Paul Boyer, in his career in science, has touched on many areas of
biochemistry. His diversity is one of his strengths. I won't detail these.
They are numerous, and one cannot be an investigator in biochemistry
today without being aware of at least some of them. The papers pre-
sented in this volume, all related to Paul's interests, bear evidence of this.
But I can mention two facets of Paul's abilities which may not come as
readily to mind as his work in biochemistry. The first is his administrative
abilities, as witness the Molecular Biology Institute of UCLA, an institute
which he organized and built to such strength and importance. The
second, which to me is as important as his concrete accomplishments in
science, is his role in the training of younger scientists, nurturing them so
to speak, at the beginning of their careers, and his influence on their
growth and development. As one who passed through his laboratory I
can attest to his rigorous thinking, his imaginative use of the tools at his
disposal, both of which he tried to impress upon his students, and his
fairness and honesty, his concern and generosity in dealing with his
co-workers, those with whom he collaborated and, just as importantly,
those whom he supervised.
I was a postdoctoral fellow in Paul's laboratory from 1956 to 1962
working, among other problems, on the nuclear magnetic resonance
spectra of proteins. The latter project may be a little surprising, but it was
Paul Boyer, then at Minnesota, who was a prime mover in obtaining an
NMR spectrometer for the school. At that time, NMR was in its infancy,
and the state-of-the-art frequency was only 56 MHz. And it was Paul who
allowed me, while I was still working in his laboratory, to "play around"
to my heart's content in the basement of the chemistry building with
proteins and NMR. So, in a very real sense, it is Paul Boyer who is
responsible for the very earliest studies on the NMR spectroscopy of
proteins.
I won't say any more, I think the fact that we have all contributed to this
work, Paul's students, past and present, his co-workers, and his friends, is
statement enough of our respect, admiration, and aifection for him.
Arthur Kowalsky
National Science Foundation
Washington, D.C.
XVll
Acknowledgment
This book is derived in part from the Proceedings of the Conference on
Enzyme Catalysis and Control, held at the University of California, Los
Angeles, on June 3 - 5 , 1983, to honor Dr. Paul Boyer on the occasion of
his sixty-fifth birthday.
This conference was made possible by financial support from Aca-
demic Press, the Albert Parvin Foundation, and the University of Califor-
nia, Los Angeles.
XIX
