energy metabolism and antibody production based on flux analysis in hybridoma

467 Pages·2008·7.72 MB·English

ANIMAL CELL TECHNOLOGY:
CHALLENGES FOR THE 21ST CENTURY
JAACT/ESACT’98 Organizing Committee
Meeting Chairman: R. Sasaki (Japan)
Meeting Secretaries: K. Ikura (Japan)
M. Nagao (Japan)
S . Masuda (Japan)
Organizing Boards:
T.Amachi(Japan) A.Ametani(Japan) K.Aramaki(Japan)
M.Carrondo(Portugal) S. Dosako(Japan) A.Enomoto(Japan)
T.Fujimori(Japan) Y.Fuke(Japan) T. Furudera(Japan)
T.Fushiki(Japan) B. Griffiths(UK) S. Hashizume(Japan)
H.Hoshi(Japan) S. Iijima(Japan) K. Ikura(Japan)
K.Inouye(Japan) K. Issiki (Japan) Y.Ikeda(Japan)
Y.Ito (Japan) S. Kaminogawa(Japan) T. Kawada(Japan)
K.Kawamura(Japan) Y.Kitagawa(Japan) J. Lehmann(Germany)
C.MacDonald(UK) S .Masuda(Japan) T.Matsuda(Japan)
R. Matsuno(Japan) O.Merten(France) K. Nagai(Japan)
K. Nagaike (Japan) M. Nagao (Japan) H . Ohigashi (Japan)
R. Sasaki (Japan) T. Sato (Japan) H . Sawada (Japan)
K. Shin (Japan) K. Shinohara (Japan) S. Shirahata (Japan)
Y. Shirai (Japan) T. Suzuki (Japan) E. Suzuki (Japan)
M.Tanokura(Japan) K.Ueda(Japan) J. Wérenne(Belgium)
K. Yagasaki (Japan) M. Yoshikawa (Japan)
Animal Cell Technology:
Challenges for
the 21st Century
Proceedings of the joint international meeting of the Japanese
Association for Animal Cell Technology (JAACT) and the European
Society for Animal Cell Technology (ESACT) 1998, Kyoto, Japan
edited by
KoujiIkura
Masaya Nagao
SeijiMasuda
and
Ryuzo Sasaki
KLUWER ACADEMIC PUBLISHERS
New York, Boston, Dordrecht, London, Moscow
eBookISBN: 0-306-46869-7
Print ISBN: 0-792-35805-8
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Contents
Preface xiii
I. Asian Biotechnology in Future
The role of Hong Kong SAR in the development of biotechnology
industry in China
A. Y. Chang 1
II.Cell Culture and Engineering
Increase in intracellular calcium influx enhances monoclonal antibody
productivity
S.-H.Park,J.-S. Park,M.-H.Nam 5
Energy metabolism and antibody production based on flux analysis in
hybridoma
T. Omasa, T. Iemara, K, Furuichi, Y. Katakura, M. Kishimoto, K.
Suga 11
Transient transfection induces early cytosolic calcium signaling in CHO-
K1 cells
A. K. Preuss, H. M. Pick, F. Wurm, H. Vogel 17
An on-line feeding strategy for fed-batch and dialysis cultures of
hybridoma cells
J. O. Schwabe, R. Pörtner, H. Märkl 23
Effects of serum and other medium components on suspension culture of
CHO cells producing tissue plasminogen activator
M.Takagi,H.C.Hia,J.H.Jang,T.Yoshida 29
Effects of osmotic pressure on metabolism of CHO cells producing tissue
plasminogen activator in adhesion culture compared with that in
suspension culture
M.Takagi,H.Hayashi,T.Yoshida 33
Cultivation of CHO cells on novel microcarriers
A.Y. Hu, Z. Zhang, M. AI-Rubeai 39
Bleeding strategy for the long-term perfusion culture of hybridoma
J. W. Yun, S. Y. Lee, B. W. Choi, H. K Oh, J. S. Lee, B. H.
Chun,W.G.Bang,T.H.Byun,S.Y.Park 45
Metabolic flux distributions in hybridoma cells at different metabolic states
P-C.Fu,A.Europa,A.Gambhir,W-S.Hu 51
On-line detection of metabolic demand and changes to it: heat flux in
continuous cultures of animal cells
Y. H. Guan, K. B. Kemp 57
Growth stimulation of CHO cells by phospholipids in serum-free culture
K. Sakai, T. Matsunaga, H. Yamaji, H. Fukuda 63
vi
A kinetic model of macromolecular monoclonal in hatch and fcii-hutch
cultures of hybridoma cells producing monoclonal antibody
J. D.Jang,J.P.Barford 69
Enhancement of antibody productivity in the near-zero specific growth rate
during glucose and glutamine-limited fed-batch culture of hybridoma cells
J.D.Jang,J.P.Barford 75
Long-term culture of CHO cells on porous microcarriers in a stirred tank
equipped with a modified cell retention system
X.Hu, C.Xiao,ZHuang, Z.Guo 8l
Continuous production and recovery of recombinant Ca++ binding
receptor from HEK 293 cells using perfusion through a packed bed
bioreactor
J.B.Kaufman,G.Wang,W.Zhang,M.A.ValleJ.Shiloach 87
111. Production ofBiologicals
Characterization of recombinant bovine lactoperoxidase produced by CHO
cells
S. Watanabe, K. Shimazaki, A. Bollen. N. Moguilevsky 93
Expression of a solubleaß heterodimeric T cell receptor in animal cells
M.Amano,M.Totsuka,K.Fujine,S.Yamada,S.Kaminogawa 99
Posttranslational modifications of recombinant von Willebrand factor:
Limitations and experimental improvement at high yield expression
B. Plaimauer, U. Schlokat, M. Himmelspach, P. L. Turecek, H. P.
Schwarz, F. G. Falkner, F. Dorner 105
Incubation with heparin as an improved harvest method for high titre
Disabled Infectious Single Cycle (DISC) HSV-2 from microcarrier
cultures
P. A. Wright, T. A. Zecchini, R. J. Smith, R. S. O’Keefe, N. K.
Slater 111
Passaging of microcarrier cultures as an alternative method for the seeding
of large scale bioreactors for the production of high titre Disabled
Infectious Single Cycle HSV-2 vaccine (DISC HSV)
T.A.Zecchini,P.A.Wright,R.J.Smith 117
The hybrid system using both promoter activation and gene amplification
for establishment of exogenous protein hyper-producing cell lines
K. Teruya, X.-Y. Dong, Y. Daimon, Y. Katakura, Y.-P. Zhang,
P.Seto,T.Miura,H.Ohashi,S.Shirahata 123
System for large scale protein expression in Bombyx mori
T. Arakawa, M. Miyazawa, S. Tomita, N. Yonemura, S. Hayasaka 129
IV. Functional Cell Lines
Protein-free culture of ras-amplified recombinant
BHK-21 cells
Y. Inoue, S. Kawamoto, M. Shoji, S. Hashizume, K. Teruya, Y.
Katakura, S. Shirahata 133
VII
V . Glycoengineering
Effect of sugar composition on the heterogeneity of antibody in hybridoma
cultivation
T.Omasa,Y.Kitamoto.J.Tanaka,Y.Katakura,M.Kishimoto.K.
Suga 139
Modulation of antigen binding of human antibody via a glycosylation by
hybridoma culture with various carbon sources
J-Y.Kim,Y.Katakura,K.Teruya,S.Shirahata 145
VI. Immunologicals, Monoclonal Antibodies andVaccines
Manipulation of cytokine production from CD8+T cells by means of amino
acid-substitutedanalogsofapeptide antigen
M. Totsuka, M. Kohyama, M. Kakchi, S. Hachimura, T. Hisatsune,
S.Kaminogawa 149
A novel effect of nivalenol (NIV) on the immune response
NIV, a trichothecene mycotoxin selectively inhibits antigen-specific Th2
responses
C-Y. Choi, H. Nakajima-Adachi, S. Kaminogawa, S. Kumagai, Y.
Sugila-Konishi 155
Human-human hybridomas producing monoclonal antibodies reactive to
house dust mites antigen
H. Kawahara, M. Maeda-Yamamoto, M. Suzuki, K. Osada, K. Tsuji 161
Establishment of human-mouse hybridomas secreting antibodies to rice-
allergens
H.Shinmoto,K.Nakahara,R.Ichikawa,M.Kobori,T.Tsushida 167
VII. Transplantation and Artificial Organs
Cell culture engineering using intelligent biomaterials
Y.Ito 171
Spatial development of mouse bone marrow cells cultivated in porous
carriers
Y.Tomimori,M.Takagi,T.Sasaki,T.Yoshida 177
Tumor promoting mechanism of biomaterials:
No involvement of mutation in cx43 gene in the tumorigenesis induced
by polyurethanes in vitro
T.Tsuchiya,M.A.Sayed,A.Nakamura 181
Xenotransplantation of cultured newborn pig thyroid tissue for the
treatment of post-radioiodine hypothyroidism in rats
I. P. Pasteur, M. D. Tronko, I. I. Drozdovich, L. M. Voitenko, S.
F. Donich 187
VIII. Gene Therapy
Improved production of retroviral vectors under serum-free conditions for
the application of gene therapy
P. A. Gerin, P. F. Searle, M. AI-Rubeai 193
viii
IX. Transgenic Animals
Large scale production of rh-albumin expressed in the milk of transgenic
cattle - An economic and technical challenge
W.Eichner,K.Scmmermeyer 199
Transgenic rabbits: A novel model for the study of atherosclerosis
J. Fan, M. Araki, L. Wu, M. Challah, H. Shimoyamada, R. M.
Lawn, H. Kakuta, H. Shikama, T. Watanabe 203
In vivo gene transfer to chicken via blastodermal cells of early
developmental embryos
S.Inada,M.Hattori,N.Fujihara 209
X. Safety and Regulations
Overview of the international endeavor toward harmonization of technical
requirements for the control of new medicines from biotechnology
T. Hayakawa 215
Reducingrisksof animal origincontaminants in cell culture
D. W. Jayme, S. R. Smith, M. Z. Plavsic 221
Cytotoxicity testing for evaluating food safety
S.Yamashoji,K.Isshiki 227
XI. Cell Regulatory Factors and Signal Transduction
Factors specifically expressed in osteoblasts
J. Kawaguchi, K. Horiuchi, H. Kudo, S. Takeshita, A. Kudo 231
Osteoclast differentiation factor is a ligand for osteoprotegerin /
osteoclastogenesis-inhibitory factor
N. Shima, H. Yasuda, N. Nakagawa, K. Yamaguchi, E. Tsuda, T.
Morinaga, T. Suda, K. Higashio 243
Erythropoietin protects neurons from ischemic damage
S.Masuda,M.Nagao,R.Sasaki 249
Monovalent antigen activates antibody/cytokine receptor chimera and
controls hematopoietic cell growth
M. Kawahara, H, Ueda, K,Tsumoto, K. Todokoro, W. Mahoney,
I. Kumagai, T. Nagamune 255
Molecular cloning of the genes that determine the invasive activities of rat
ascites hepatoma cells
Y.Miura,S.Totsuka,Q.Lee,K.Yagasaki 261
Antitumor protein (AP) from a mushroom induced apoptosis to
transformed human keratinocyte by controlling the status of pRB, c-myc,
cyclin E-CDK2, and p21WAF1 in the GI/S transition
Y.Kawamura,M.Manabe,K.Kitta 265
Transformation of NIH/3T3 and C3H/10T1/2 cells by citrinin and
ochratoxin A
A. Mehta, N. Kitabatake 273
ix
Senescence induction in cancer cells
Y. Katakura, T. Miura. N. Uehara, E. Nakata, S. Shirahata 279
Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I
and/or IGF-II
D. Li, S. Hettle, J. McLean, C. MacDonald 283
Targeted disruption of a mitochondrial transcription factor Ain the chicken
DT40 cell lines
Y. Matsushima, K. Malsumura, S. Isaii, Y. Kitagawa 289
Expression and characterization of mouse epidermal transglutaminase in
baculovirus-infected insect cells
K.Hitomi,K.Ikura,M.Maki 295
Effect of tea polyphenols on degranulation in human mature basophils
differentiated with IL-4
H. Tachibana, Y. Sunada, T. Hara, K. Yamada 301
Counteraction of the active form of vitamins A and D on up-regulation of
adipocyte differentiation with PPARγligand, A thiazolidinedione, in 3T3-
L1c ells
Y. Hida, T. Kawada, S. Kayahashi, T. Ishihara, T. Fushiki 307
CCK regulation of monitor peptide gene expression in pancreatic acinar
AR42J cells
T. Kinouchi, S. Tsuzuki, C. Minami, Y. Hayashi, E. Sugimoto, T.
Fushiki 313
Detection of mitochondrial regulatory region RNA in cultured cells and
differentiated tissue cells: Theimplications for cellular growth control
N. Nakamichi, M. Ito, T. Matsumura 319
Effect of ovarian steroids and oxytocin on local production of
prostaglandin E, prostaglandin F and endothelin-1 in cow oviductal
2 2α
epithelial cell monolayers
M.P.B.Wijayagunawardane,Y.H.Choi,A.Miyamoto,H.
Kamishita,AY.Kojima,M.Takagi,K.Sato 325
TGF-βinduced cellular senescence in cancer cells is reversible and
operates via two separate and independent pathways
T.Miura, Y.Katakura,E.Nakata,N.Uehara,S.Shirahata 331
Molecular cloning and characterization of specific genes involved in
cellular senescence
N.Uehara,Y.Katakura,T.Miura, S.Shirahata 337
Regulatory mechanisms of granulosa cell apoptosis in ovarian follicle
atresia
N. Manabe, Y. Kimura, K. Uchio, C. Tajima. H. Matsusnita, M.
Nakayama, M. Sugimoto, H. Miyamoto 343
Enhancement of FcεRI expression by hydrocortisone induces the
upregulation of FcεRIγ mRNA in the human leukemia cell line KU812
T. Hara, H.Tachibana,K.Yamada 349