Table Of ContentProgress in Molecular and Subcellular Biology
Editors
Christophe Lamaze · Ian Prior
Endocytosis
and
Signaling
Progress in Molecular and Subcellular Biology
Volume 57
Editor-in-chief
Werner E. G. Müller, Mainz, Germany
Series editors
Heinz C. Schröder, Mainz, Germany
Ðurðica Ugarković, Zagreb, Croatia
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Christophe Lamaze Ian Prior
(cid:129)
Editors
Endocytosis and Signaling
123
Editors
Christophe Lamaze IanPrior
MembraneDynamics andMechanicsof Department ofCellular andMolecular
Intracellular SignalingLaboratory, Physiology,Institute of Translational
CNRSUMR3666, INSERMU1143, Medicine
Institut Curie—Centre deRecherche University of Liverpool
PSLResearch University Liverpool, UK
Paris, France
ISSN 0079-6484 ISSN 2197-8484 (electronic)
Progressin Molecular andSubcellular Biology
ISBN978-3-319-96703-5 ISBN978-3-319-96704-2 (eBook)
https://doi.org/10.1007/978-3-319-96704-2
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Preface
It is now widely appreciated that cell signalling is highly context dependent. The
location and concentration of signalling nodes regulate their activation cycles and
engagement with distinct effector pathways. Whilst many cell signalling pathways
are initiated from the cell surface, endocytosis provides an opportunity for modu-
lation oftheoutputofsignalling networks.Inthisbook,wewillhighlighthowthe
endosomalsystemhelpstoorganiseandregulatesignallingpathways.Inaseriesof
reviews,wewillfirstlyfocusontheendocyticandendosomalsystemanddescribe
how these subcellular platforms sort and regulate a wide range of signalling
pathway components and phenotypic outputs. We will then review the latest sci-
entific insights into how endocytic trafficking and subcellular location modulate a
set of major pathways essential for normal cellular function and organism
development.
Paris, France Christophe Lamaze
Liverpool, UK Ian Prior
v
Contents
1 The Endosomal Network: Mediators and Regulators
of Endosome Maturation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Maria Podinovskaia and Anne Spang
2 Integration of the Endocytic System into the Network
of Cellular Functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Noga Budick-Harmelin and Marta Miaczynska
3 GTPases Rac1 and Ras Signaling from Endosomes . . . . . . . . . . . . 65
Francesc Tebar, Carlos Enrich, Carles Rentero and Thomas Grewal
4 ESCRT and Membrane Protein Ubiquitination . . . . . . . . . . . . . . . 107
Simona M. Migliano and David Teis
5 Retromer and Its Role in Regulating Signaling
at Endosomes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Matthew N. J. Seaman
6 The Lysosome and Intracellular Signalling. . . . . . . . . . . . . . . . . . . 151
Geoffrey G. Hesketh, Lena Wartosch, Luther J. Davis,
Nicholas A. Bright and J. Paul Luzio
7 Interplay of Endocytosis and Growth Factor
Receptor Signalling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 181
Rachel Moore, Marta Giralt Pujol, Zhou Zhu and Elizabeth Smythe
8 Role of the Endocytosis of Caveolae in Intracellular Signaling
and Metabolism . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
Olivia Muriel, Miguel Sánchez-Álvarez, Raffaele Strippoli
and Miguel Angel del Pozo
9 EGFR Trafficking in Physiology and Cancer . . . . . . . . . . . . . . . . . 235
Giusi Caldieri, Maria Grazia Malabarba, Pier Paolo Di Fiore
and Sara Sigismund
vii
viii Contents
10 Evolving View of Membrane Trafficking and Signaling Systems
for G Protein-Coupled Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . 273
Silvia Sposini and Aylin C. Hanyaloglu
11 Endosomal Trafficking During Mitosis and Notch-Dependent
Asymmetric Division. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 301
Alicia Daeden and Marcos Gonzalez-Gaitan
Chapter 1
The Endosomal Network: Mediators
and Regulators of Endosome Maturation
MariaPodinovskaiaandAnneSpang
Contents
1.1 EndosomeMaturationOverview .............................................. 2
1.2 EndosomeFunctions ....................................................... 5
1.3 MediatorsofEndosomeMaturation ........................................... 7
1.3.1 Phosphoinositides ................................................... 7
1.3.2 RabGTPases ....................................................... 8
1.3.3 Tethers ............................................................. 9
1.3.4 ESCRTs ............................................................ 10
1.3.5 V-ATPase .......................................................... 10
1.3.6 AdditionalMediatorsofVacuolarpH ................................... 11
1.3.7 Cytoskeleton ........................................................ 12
1.4 CoordinationofEndosomeMaturation ........................................ 13
1.4.1 SortingCompartments ................................................ 14
1.4.2 RabConversion ..................................................... 15
1.4.3 Rab5Regulation ..................................................... 16
1.4.4 Rab7Regulation ..................................................... 16
1.4.5 PIPConversion ...................................................... 17
1.4.6 PIPRegulation ...................................................... 17
1.4.7 V-ATPaseRegulation ................................................. 18
1.5 ContributionsofOtherOrganellestoEndosomeMaturation ....................... 20
1.5.1 ER ................................................................ 20
1.5.2 LipidDroplets ...................................................... 21
1.5.3 CrosstalkwiththeAutophagyPathway ................................. 22
1.6 PerturbationsofEndosomeMaturationinDisease ............................... 22
1.7 Conclusions ............................................................... 24
References ..................................................................... 24
Abstract Endocytosisisameansforthecelltosampleitsenvironmentfornutrients
and regulate plasma membrane (PM) composition and area. Whereas the majority
ofinternalizedcargoisrecycledbacktothecellsurface,selectmaterialissenttothe
B
M.Podinovskaia·A.Spang( )
Biozentrum,UniversityofBasel,Klingelbergstrasse70,4056Basel,Switzerland
e-mail:[email protected]
M.Podinovskaia
e-mail:[email protected]
©SpringerInternationalPublishingAG,partofSpringerNature2018 1
C.LamazeandI.Prior(eds.),EndocytosisandSignaling,ProgressinMolecular
andSubcellularBiology57,https://doi.org/10.1007/978-3-319-96704-2_1
2 M.PodinovskaiaandA.Spang
lysosomefordegradation.Endosomesfurtherplaymajorrolesincentralcellactiv-
ities as diverse as establishment of cell polarity and signaling, lysosomal storage
andimmunity.Thecomplexityofendosomalfunctionsisreflectedbytheextensive
changestoendosomepropertiesastheymature.Theidentityofindividualendosomes
isinfluencedbythepresenceofspecificRabGTPasesandphosphoinositides(PIPs),
whichcoordinatemembranetrafficandfacilitateendosomalfunctions.Motorsand
tethersdirecttheendosomestotherequiredlocationsandmoderatefusionwithother
organelles.Themaintenanceoftheelaborateendosomalnetworkissupportedbythe
ERandthetrans-Golginetwork(TGN),whichpromotetheexchangeofmembrane
components, provide enzymes, and assist with signaling. Additionally, V-ATPase
is emerging as an underappreciated coordinator of endosome maturation and cell
signaling. The inputs of the various mediators of endosome maturation are tightly
regulated and coordinated to ensure appropriate maintenance and functioning of
endosomesateachstageofthematurationprocess.Perturbationsinendosomemat-
urationareimplicatedindevastatingdiseases,suchasneurodegenerationandcancer,
andtheendosomematurationprocessesaremanipulatedandexploitedbyintracellu-
larpathogenstomeettheirownneeds.Agreaterunderstandingofcoordinationand
fine-tuningofendosomematurationwillhelpusaddressvariouspathologiesmore
effectively.
Abbreviations
EE Earlyendosome
CI-M6PR Cation-independentmannose-6-phosphatereceptor
ILV Intraluminalvesicle
LE Lateendosome
MVB Multivesicularbody
PIP Phosphoinositidephosphate
PM Plasmamembrane
TfR Transferrinreceptor
TGN Trans-Golginetwork
1.1 EndosomeMaturation Overview
Plasma membrane (PM) turnover and ingestion of extracellular material relies on
a variety of endocytic pathways. The best studied pathway is clathrin-dependent
endocytosis,whichentailsrecognitionofcargoviaspecificreceptors,development
ofclathrin-coatedpit,andformationofsubsequentclathrin-coatedvesicle(Doherty
and McMahon 2009). Indiscriminate fluid sampling is concerted by macropinocy-
tosis (Buckley and King 2017). A number of alternative endocytic pathways have
beendescribedandmaybecategorized bytheirPM-associatedmediators,suchas
