ELx808 Absorbance Microplate Reader Operator's Manual

222 Pages·2006·2.13 MB·English

Absorbance Microplate Reader
ELx808™
Operator’s Manual
.
ELx808™
Absorbance Microplate Reader
Operator’s Manual

April 2006
© 2006
Part Number 7341000
Revision L
BioTek® Instruments, Inc.
Notices

BioTek Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
All Rights Reserved
© 2006, BioTek Instruments, Incorporated. No part of this publication may be reproduced,
transcribed, or transmitted in any form, or by any means electronic or mechanical, including
photocopying and recording, for any purpose other than the purchaser’s use without written
permission of BioTek Instruments, Inc.
Trademarks
BioTek® is a registered trademark, and Extensions® Define Protocol, ELx808™, Gen5™,
KCjunior™, and KC4™ are registered trademarks or trademarks of BioTek Instruments, Inc. All
other trademarks are the property of their respective holders.
Restrictions and Liabilities
Information in this document is subject to change, and does not represent a commitment by
BioTek Instruments, Inc. Changes made to the information in this document will be incorporated
in new editions of the publication. No responsibility is assumed by BioTek for the use or
reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.
ii Preface
Table of Contents

Preface..................................................................................................................i
Notices........................................................................................................................................ii
All Rights Reserved.............................................................................................................ii
Trademarks.........................................................................................................................ii
Restrictions and Liabilities...................................................................................................ii
Contact Information.................................................................................................................viii
Document Conventions.............................................................................................................ix
Revision History..........................................................................................................................x
Intended Use Statement..........................................................................................................xiii
Specimen Preparation......................................................................................................xiii
Repackaging and Shipping...............................................................................................xiii
Warnings and Precautions.......................................................................................................xiv
CE Mark Information................................................................................................................xvi
Electromagnetic Interference and Susceptibility.....................................................................xviii
Safety Symbols........................................................................................................................xix
Chapter 1: Introduction ...................................................................................1-1
Introducing the ELx808 Absorbance Microplate Reader.........................................................1-2
Quality Control........................................................................................................................1-2
Hardware Features.................................................................................................................1-3
Software Features..................................................................................................................1-3
Specifications..........................................................................................................................1-4
Standard Model...............................................................................................................1-4
Ultraviolet/Incubator Model..............................................................................................1-5
Package Contents..................................................................................................................1-7
Optional Accessories..............................................................................................................1-8
Product Support & Service.....................................................................................................1-9
Contacting the Technical Assistance Center...................................................................1-9
Returning the Instruments for Service/Repair..................................................................1-9
Chapter 2: Installation.....................................................................................2-1
Unpack and Inspect the Instrument........................................................................................2-2
Select the Operating Environment..........................................................................................2-3
Install the Filter Wheel............................................................................................................2-4
ELx808 Operator’s Manual iii
Check/Adjust the Power Input Voltage Setting.......................................................................2-6
Connect Power.......................................................................................................................2-6
Install the Printer.....................................................................................................................2-7
Printers............................................................................................................................2-8
Cable...............................................................................................................................2-8
Parallel Port Pin Definition...............................................................................................2-8
Turn on the Reader and Run a System Test..........................................................................2-9
Check/Adjust the Reader’s Filter Table................................................................................2-10
Configure Utility Options.......................................................................................................2-11
SETUP Options.............................................................................................................2-11
OUTPUT Options..........................................................................................................2-12
REPORT Type...............................................................................................................2-13
READ Options...............................................................................................................2-14
Read Speed...................................................................................................................2-14
(Optional) Connect the Host PC...........................................................................................2-15
Attach the Serial Cable..................................................................................................2-15
Install Software on the Host PC.....................................................................................2-15
Set Communication Parameters....................................................................................2-15
Change the Baud Rate on the ELx808..........................................................................2-16
Serial Port Pinout Description........................................................................................2-17
After Installation and Setup, Verify Performance..................................................................2-18
Before Repackaging the Instrument.....................................................................................2-18
Chapter 3: Operation .......................................................................................3-1
Introduction.............................................................................................................................3-2
The Keypad.....................................................................................................................3-2
The Startup Screen.........................................................................................................3-3
The Main Menu Screen...................................................................................................3-3
Defining Assays......................................................................................................................3-5
Selecting an Assay..........................................................................................................3-5
Assay Name....................................................................................................................3-6
Defining the Method, Map, Formula, and Curve..............................................................3-7
METHOD.........................................................................................................................3-7
Read Type................................................................................................................3-8
Delay in First Read...................................................................................................3-8
Incubation Temperature...........................................................................................3-9
Single or Dual Wavelength.......................................................................................3-9
iv Preface
MEAS Selection.....................................................................................................3-10
Number of Kinetic Reads/Kinetic Duration Selection..............................................3-10
Kinetic Interval .......................................................................................................3-11
Kinetic Number of Reads........................................................................................3-11
Kinetic Duration......................................................................................................3-12
Shake Mode Selection............................................................................................3-12
Shake Time............................................................................................................3-13
Shake Speed..........................................................................................................3-13
Kinetic Data Analysis Selection..............................................................................3-14
Number of Kinetic Points Selection........................................................................3-14
Onset OD Selection................................................................................................3-15
Linear Scanning......................................................................................................3-15
MAP...............................................................................................................................3-16
Map Generation......................................................................................................3-17
Mapping Direction...................................................................................................3-18
Replication Direction...............................................................................................3-18
Examples of Mapping Directions............................................................................3-19
Start Mapping at Well Location...............................................................................3-20
Selecting a Blank Map............................................................................................3-20
Blank Map Definitions.............................................................................................3-21
Constant Blank Value Entry....................................................................................3-22
Number of Blanks...................................................................................................3-22
Selecting a Blank Location.....................................................................................3-23
Number of Standards.............................................................................................3-23
Number of Standard Replicates..............................................................................3-24
Average Standards.................................................................................................3-24
Standard Concentration..........................................................................................3-25
Reuse of Standard Curves.....................................................................................3-26
Number of Controls................................................................................................3-27
Control Type...........................................................................................................3-28
Number of Control Replicates................................................................................3-28
Location of Controls................................................................................................3-29
Valid Well Locations...............................................................................................3-29
Number of Samples................................................................................................3-29
Number of Sample Replicates................................................................................3-30
Sample Location.....................................................................................................3-30
ELx808 Operator’s Manual v
FORMULA.....................................................................................................................3-31
Validation Formula Examples.................................................................................3-31
Formula Type.........................................................................................................3-32
Validation Type Selection.......................................................................................3-33
Formula Entry.........................................................................................................3-34
Number of Required Controls/Blanks.....................................................................3-36
Cutoff Formulas......................................................................................................3-37
Greyzone Entry.......................................................................................................3-38
Positive/Negative Calls for Cutoff...........................................................................3-38
Examples................................................................................................................3-39
Transformation Formulas.......................................................................................3-40
Transformation Formula Definition.........................................................................3-40
Transformation Scope Variable..............................................................................3-41
CURVE..........................................................................................................................3-43
Curve Fit.................................................................................................................3-43
Edit Standard Outliers............................................................................................3-45
Axis Selection.........................................................................................................3-46
Extrapolation of Unknowns.....................................................................................3-46
Panel Assays.................................................................................................................3-47
Reading a Microplate............................................................................................................3-50
Select Assay..................................................................................................................3-50
Run-Time Prompts........................................................................................................3-50
Enter Number of Samples.............................................................................................3-52
Enter Plate ID................................................................................................................3-52
Enter Sample ID............................................................................................................3-53
Prompts for Well Location..............................................................................................3-53
Beginning the Plate Read..............................................................................................3-53
Printing Reports and Assay Lists..........................................................................................3-54
Result............................................................................................................................3-55
Editing Standard Outliers...............................................................................................3-55
Printing Results.............................................................................................................3-56
Map...............................................................................................................................3-57
Assay.............................................................................................................................3-57
List.................................................................................................................................3-57
Chapter 4: Instrument Qualification...............................................................4-1
Recommendations for Achieving Optimum Performance.......................................................4-2
vi Preface
Installation Qualification (IQ)...................................................................................................4-3
Recommended Qualification Schedule (OQ/PQ)....................................................................4-4
Qualification Procedures.........................................................................................................4-5
Initiating Tests Via the Utility Option.......................................................................................4-6
“SYSTEM” (System Test)................................................................................................4-7
“CHKSUM” (Checksum Test)........................................................................................4-10
“CALPLTE” (Absorbance Plate Test).............................................................................4-10
Absorbance Plate Test.........................................................................................................4-11
Description.....................................................................................................................4-11
Requirements................................................................................................................4-12
Defining the Test Plate Parameters...............................................................................4-13
Running the Absorbance Plate Test..............................................................................4-14
Results & Troubleshooting Tips.....................................................................................4-16
Empty Carrier Test................................................................................................................4-18
Liquid Testing.......................................................................................................................4-19
Stock Solution Formulation............................................................................................4-20
Liquid Test 1..................................................................................................................4-22
Liquid Test 2..................................................................................................................4-24
Liquid Test 3..................................................................................................................4-27
Chapter 5: Preventive Maintenance................................................................5-1
Recommended Maintenance Schedule..................................................................................5-2
Overview..........................................................................................................................5-2
Warnings and Precautions......................................................................................................5-3
Clean Exposed Surfaces........................................................................................................5-4
Inspect and Clean the Wavelength Filters..............................................................................5-5
(Optional) Lubricate Robotic Components..............................................................................5-6
Replace the Lamp and Clean the Contacts............................................................................5-8
Appendix A: Decontamination.......................................................................A-1
Appendix B: Computer Control.....................................................................B-1
Appendix C: Error Codes...............................................................................C-1
Appendix D: Report Format...........................................................................D-1
Appendix E: Programming a New Assay.......................................................E-1
Appendix F: Adjusting the Power Input Voltage Setting..............................F-1
ELx808 Operator’s Manual vii
Contact Information

BioTek Instruments, Inc.
Highland Park, P.O. Box 998
Winooski, Vermont 05404-0998 USA
Customer Service and Sales
Internet: www.biotek.com
Phone: [phone] (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: [phone]
E-Mail: [email protected]
Service/TAC
Phone: [phone] (toll free in the U.S.)
802-655-4740 (outside the U.S.)
Fax: [phone]
E-Mail: [email protected]
European Coordination Center
BioTek® Instruments GmbH
Kocherwaldstrasse 34
D-74177 Bad Friedrichshall
Germany
Internet: www.biotek.de
Phone: [phone]
Fax: [phone]
E-Mail: [email protected]
viii Preface