Table Of ContentUniversity of Granada
Faculty of Medicine
Department of Microbiology
Epidemiological Studies on Zoonotic Leishmaniasis
and New Trials for Studying the Effect of Melatonin
on the Parasite
Ehab Kotb Abd Elghany Elmahallawy
A thesis submitted for the fulfillment of the degree of
International Doctor of Philosophy (PhD) in Clinical Medicine
and Public Health
Granada, 2015
Editor: Universidad de Granada. Tesis Doctorales
Autor: Ehab Kotb Abd Elghany Elmahallawy
ISBN: 978-84-9125-544-4
URI: http://hdl.handle.net/10481/42676
Epidemiological Studies on Zoonotic Leishmaniasis
and New Trials for Studying the Effect of Melatonin
on the Parasite
Instituto de
Neurociencias Facultad de Medicina Universidad de Granada
Hospital virgen de las Nieves Departamento de Departamento de
- Granada Farmacología Microbiología
D. Ahmad Mhmad Agil Abdalla, Profesor Titular de farmacología de la Facultad de medicina
Universidad de Granada, D. Jose Gutiérrez-Fernández, Catedrático de Microbiología de la
Facultad de medicina Universidad de Granada y D. Javier Rodriguez-Granger, doctor del
servicio de Microbiología y Parasitología, Hospital Universitario Virgen de las Nieves Granada
certifica que: Don. Ehab Kotb Abd Elghany Elmahallawy, que ha realizado bajo mi dirección
en el Departamento de Farmacología y Departamento de Microbiología de la Facultad de
medicina Universidad de Granada y Servicio de Microbiología y Parasitología, Hospital
Universitario Virgen de las Nieves, Granada, el presente trabajo de investigación, titulado:
‘’Epidemiological Studies on Zoonotic Leishmaniasis and New Trials for Studying the
Effect of Melatonin on the Parasite‘’, que ha sido objeto de su tesis doctoral, reuniendo las
condiciones necesarias para optar al grado de doctor.
Granada, 11 Septiembre de 2015
Dirigida por los Directores:
Fdo.: Ahmad Agil Abdalla
Fdo.: Jose Gutiérrez-Fernández
Fdo.: Javier Rodriguez-Granger
El interesado: Fdo.: Ehab Kotb Abd Elghany Elmahallawy
Dr. Ahmad Mhmad Agil Abdalla, Professor of Pharmacology, Faculty of Medicine University
of Granada, Dr. Jose Gutierrez-Fernandez, Professor of Microbiology, Faculty of Medicine
University of Granada and Dr. Javier Rodriguez-Granger, doctor service Microbiology and
Parasitology, University Hospital Virgen de las Nieves Granada certify that: Mr. Ehab Kotb Abd
Elghany Elmahallawy, who has done under my direction in the Department of Pharmacology
and Department of Microbiology, Faculty of Medicine and University of Granada Department of
Microbiology and Parasitology, University Hospital Virgen de las Nieves, Granada, the present
research entitled: '' Epidemiological Studies on Zoonotic leishmaniasis and New Trials for
Studying the Effect of Melatonin on the Parasite '' which has been the subject of his doctoral
thesis, gathering the necessary conditions for the degree of doctor.
Granada, 11 Septiembre de 2015
Directed by Directors:
Signed: Ahmad Agil Abdalla
Signed: Jose Gutiérrez-Fernández
Signed: Javier Rodriguez-Granger
Interested author: Ehab Kotb Abd Elghany Elmahallawy
El doctorando Ehab Kotb Abd Elghany Elmahallawy y los directores de la tesis Ahmad
Mhmad Agil Abdalla, Jose Gutiérrez-Fernández, y Javier Rodriguez-Granger,
garantizamos, al firmar esta tesis doctoral, que el trabajo ha sido realizado por el doctorando bajo
la dirección de los directores de la tesis y hasta donde nuestro conocimiento alcanza, en la
realización del trabajo, se han respetado los derechos de otros autores a ser citados, cuando se
han utilizado sus resultados o publicaciones.
Granada, 11 Septiembre de 2015
Dirigida por los Doctores:
Fdo.: Ahmad Agil Abdalla
Fdo.: Jose Gutiérrez-Fernández
Fdo.: Javier Rodriguez-Granger
El interesado:
Fdo.: Ehab Kotb Abd Elghany Elmahallawy
The doctoral candidate Ehab Kotb Abd Elghany Elmahallawy and directors of the thesis
Ahmad Mhmad Agil Abdalla, Jose Gutierrez-Fernandez and Javier Rodriguez-Granger,
guarantee, by signing this document that the work has been performed by the doctoral student
under the direction of thesis directors and as far as our knowledge reaches, in the performance of
work, have respected the rights of others to be mentioned when the results have been used or
publications.
Granada, 11 Septiembre de 2015
Directed by Directors:
Signed: Ahmad Agil Abdalla
Signed: Jose Gutiérrez-Fernández
Signed: Javier Rodriguez-Granger
Interested author:
Signed: Ehab Kotb Abd Elghany Elmahallawy
Declaration by author
This thesis is composed of my original work, and contains no material previously published or
written by another person except where due reference has been made in the text. I have clearly
stated the contribution by others to jointly-authored works that I have included in my thesis.
I have clearly stated the contribution of others to my thesis as a whole, including statistical
assistance, survey design, data analysis, significant technical procedures, professional editorial
advice, and any other original research work used or reported in my thesis. The content of my
thesis is the result of work I have carried out since the commencement of my research higher
degree candidature and does not include a substantial part of work that has been submitted to
qualify for the award of any other degree or diploma in any university or other tertiary
institution. I have clearly stated which parts of my thesis, if any, have been submitted to qualify
for another award.
I acknowledge that copyright of all material contained in my thesis resides with the copyright
holder(s) of that material. Where appropriate I have obtained copyright permission from the
copyright holder to reproduce material in this thesis.
Granada, 11 Septiembre de 2015
Directed by Directors:
Signed: Ahmad Agil Abdalla
Signed: Jose Gutiérrez-Fernández
Signed: Javier Rodriguez-Granger
Interested author:
Signed: Ehab Kotb Abd Elghany Elmahallawy
Dedication
This work is dedicated to Soul of my father, my
mother and my sisters and all family, and to the
people who dedicate their lives to seek for the
truth…
Acknowledgement
Description:Cats were examined clinically for any lesion together with routine hematochemical examination. For the complete blood count (CBC), Pentra60 Horiba