Table Of ContentG. Berghaus . B. Brinkmann
C. Rittner· M. Staak (Eds.)
DNA-Technology
and Its Forensic Application
With 91 Figures
Springer-Verlag
Berlin Heidelberg New York
London Paris Tokyo
Hong Kong Barcelona
Budapest
Prof. Dr. rer. bioI. hum . G. B ERGHAUS
Universitat Koln
Institut fur Rechtsmedizin , Melatengiirtel60
W -5000 Koln 30, FRG
Prof. Dr. med. B. BRINKMANN
W estfalisch e Wilhelms-Unive rsitat
lnstitut fur R echtsmedizin , von Esmarch-StraBe 86
W-4400 Munster, FRG
Prof. Dr. med . C. RITINER
loha nnes-Gute nberg-Universitat
Institut fur Rechtsmedizin, Am Pulverturm 3
W -65.DO Mainz, FRG
Prof. Dr . med . M . STAAK
Universitat Koln
Institut fur Rechtsmedizin, Melatengurte l60
W-5000 Koln 30, FRG
Proceedings of an International Symposium. Cologne, September 13-14, 1990
ISBN- I 3:9 78-3-540-54035-9 e-ISBN- 13: 978-3-642-76632-9
001: 10.10071978-3-642-76632-9
Li braI")' of Congress Cataloging-in-Publication Data. DNA-Technology and its forensic application I
edited by G. Berghaus ... let ai. ]. p. em. Based on a symposium held during the 69th Congress of the
German Society of Legal Medicine, on Sept. 13-14, 1990, Cologne, Germany. Includes b bi liographical
references and index. ,1SBN 3-540-5403~ (alk. paper). - - ISBN 0-387-5403S-O (alk. paper). I. DNA
fingerprints-Congresses. 2. Forensic genetics -- Technique-Congresses. I. Bcrghaus. G. II . German
Society of Legal Medicine. Congress (69th: 1990: Cologne, Germany) (DNLM : I. DNA- analysis--con-
gresses. 2. Forensic Medicine-Congresses. W 750 D7SS 19901 RA IOS7.SS.D67 1991 614'. 1--dc20
DNLM/DLe for Library of Congress.
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C Springer-VerIag Berlin Heidelberg 1991
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Preface
This volume contains the scientific contributions presented at an International
Symposium on "DNA-technology and its forensic application", held within the
69th Congress of the German Society of Legal Medicine on September 13-14,
1990 in Cologne, Germany.
The contributions to the symposium covered all aspects of the application of
DNA-polymorphisms to paternity and to biological stain analysis. This included
review lectures and basic lectures on biostatistical approaches and legal questions
as well as many new technical approaches.
We would like to thank the authors of the articles contained in this book and to
Springer-Verlag for having made such a quick publication possible.
We hope, that these contributions will give the reader a survey of the most re-
cent developments in the field of forensic DNA-technology.
Summer 1991 The Editors
Contents
Review Lectures
Principles and Recent Advances in DNA Fingerprinting
AJ. Jeffreys, N.J. Royle, I. Patel, J.AL. Armour, A. MacLeod, -
A Collick, I.C. Gray, R. Neumann, M. Gibbs, M. Crosier, M. Hill,
E. Signer, and D. Monckton . . . . . . . . . . . 3
DNA Fingerprinting as a Tool of Paternity Testing in Germany
C. Rittner, U. Schacker, and P.M. Schneider . . . . . . 20
Genetic1YPing of Biological Evidence
Using the Polymerase Chain Reaction
G.F. Sensabaugh. . . . . . . 33
A Critical Review of the Forensic Application of DNA Technology
B. Brinkmann, P. Wiegand, and S. Rand . . . . . . . . 41
DNA Methylation: A Defense Mechanism
Against the Expression of Foreign DNA?
W. Doerfler . . . . . . . . . . 50
Juristische Aspekte der forensischen DNA-Analyse
G. Kohlmann. . . . . . . . . . . . . 59
peR-Technology
Research Regarding the Polymerase Chain Reaction
for Forensic Utility - HLA-DQa Locus and AMP-FLPs
B. Budowle, C.T. Comey, J.M. Jung, and A.M. Giusti. 71
DNA Typing Using PCRAmplified Fragments
in the HLA Class I Region
J. Holtz, K. SchOnbom-Sobolewski, and S. Urmetz . 79
PCR-Typing of the Human HLA-DQa Locus:
Population Genetics and Application in Forensic Casework
P.M. Schneider, A. Veit, and C. Rittner . . . . . . 85
VITI Contents
DNA Amplification (PCR) of Several Hypervariable Loci
in Disputed Paternity Cases
E. Trabetti, D. De Leo, P. Gasparini, P.P' Pignatti, and M. Marigo 92
Amplification of a Highly Polymorphic VNTR Segment,
Localized on the Ha-Ras Gene by PCR
H. Haas, M. Prinz, M. Staak, and G. Berghaus . . . . . . . . . 96
Methodology, Data Bases
Reliability of Restriction Enzyme Digestions of Genomic DNA
for the Generation of DNA Fingerprints
U. Schacker, T. Kaufmann, P.M. Schneider, and C. Rittner . . 103
Concept for a More Precise Definition of the Polymorphism YNH24
C. Puers, S. Rand, and B. Brinkmann . . . . . . . . . . . . 109
Evaluation of Variable Number of Tandem Repeat (VNTR) Alleles
in Mother-Child Combinations
G. Holmlund, and B. Lindblom . . . . . . . . . . . . . . 116
A Proposed Combination of Single Locus Probes
for Multiple Rehybridization
P. Wiegand, S. Rand, andB. Brinkmann . . . . 121
DNA-ProfIles with HVR-Probes - Allele Frequencies of HinfI-RFLPs
Using pHINS310, pMUC7 and pMR24/1 in a Population Sample
from Hannover and Lower Saxony
T. Rothamel, W. Keil, and H.D. Troger. . . . . . . . . 128
Analysis of Allele Frequencies of Tho Polymorphic Loci
in a North-East Italian Population
P. Fattorini, A.P. Callegaro, P. Florian, D. Fabbro, T. Frisan,
B.M. Altamura, and G. Graziosi. . . . . . . . . . 134
Digoxigenin-DNA Probes for Detecting Human VNTR Polymorphism
N. Dimo-Simonin, C. Brandt-Casadevall, andH.-R. Gujer . . .. 141
Practical Use: Paternity
The Application of Four Single Locus Probes
in Cases of Disputed Paternity
J.A. Thompson, P.J. Lincoln, C.P. Phillips, D. Syndercombe Court,
and P.H. Watts . . . . . . . . . . . . . . . . . . . 147
Contents IX
The Practical Significance of Human Genetic RFLP-Systems
in Paternity Testing
D. Krause, R. Szibor, W. Kuchheuser, and R. Bruckner 153
Individual Diagnostics by DNA Fingerprinting
M. Rose, L. Roewer, O. Prokop, and G. Geserick . . 157
Paternity Analysis in a Deficient Family by DNA Profiling
(Single-Locus and Multi-Locus Probes)
E. Iten and R. Pflugshaupt ....... . . . . . . . . 160
Paternity Tests in 10-12 Weeks Old Fetuses
by Mean of Single-Locus-DNA-Polymorphisms
L. Henke, G. Hummelsheim, and J. Henke . 165
DNA Fingerprinting for the Protection of Endangered Species
J. Mathe, R. Wolfes, and A. Seitz . . . . . . . . . 170
Practical Use: Stain Analysis
Treatment of Bloodstains for Simultaneous Application of Serological
and DNA 'TYping Methods
M. Prinz, T. Ohshima, M. Staak, and G. Berghaus . . . . . . . . 175
DNA Extraction and RFLP Analysis
of Bloodstains on a Variety of Textiles -
Investigation of Various Extraction Procedures
R. Scheithauer, and H.-J. Weisser . . . . 181
Stability of DNA in Brain Cortex After Long Post Mortem Periods
B. Ludes, P. Mangin, andA.J. Chaumont. . . . . . . .. 187
The Identification of a Charred Body by Oligonucleotide Fingerprinting
with the (GTG)s Probe
H. Poche, G. Wrobel, V. Schneider, and J.T. Epplen . . . . . . . 192
Biostatistics
Biostatistical Evaluation of Evidence
from Single Locus Hypervariable DNA Probes:
Tests of Independence of Loci
J.w. Morris and C.H. Brenner . . . . . 199
MLP and SLP Band Patterns and Probabilities ofIdentity
K. Hummel and N. Fukshansky . . . . . . . . . 203
x Contents
Efficiency of Multilocus Probes in Mother-Child-Putative Father Cases
N. Fukshansky and K. Hummel . . . . . . . . . . . . . . 208
The Proposal of Evett et al. for Paternity Calculations
in Normal Trio Cases Using MLP Profiles and the Freiburg Model
of a Multiple Diallelism: A Comparison
K. Hummel and N. Fukshansky . . . . . . . . . . . . . . 212
A Contribution to the Computer-Aided Analysis
of DNA Fingerprint Patterns
A. Kelch, H.-P. Kinzl, K. Trommler, and U. Ziegler . . . . . . . 221
List of Contributors
Altamura, B.M. 134 Hill,M. 3 Rand,S. 41,109,121
Annour, J.A.L. 3 Holmlund, G. 116 Rittner, C. 20,85,103
Berghaus, G. 96, 175 Holtz,J. 79 Roewer,L. 157
Brandt-CasadevaIl, C. Hummel, K. 203,208, Rose, M. ~157
141 212 Rothamel, T. 128
Brenner, C.H. 199 Hummelsheim, G. 165 Royle,NJ. 3
Brinkmann, B. 41, 109, Iten, E. 160 Schacker, U. 20, 103
121 Jeffreys, AJ. 3 Scheithauer, R. 181
Bruckner, R. 153 Jung, J.M. 71 Schneider, P.M. 20,85,
Budowle, B. 71 Kaufmann, T. 103 103
Callegaro, A.P. 134 Keil, W. 128 Schneider, V. 192
Chaumont, A.J. 187 Kelch, A. 221 SchOnbom-Sobolewski,
Collick, A. 3 Kinzl, H.P. 221 K. 79
Corney, C.T. 71 Kohlmann, G. 59 Seitz, A. 170
Crosier, M. 3 Krause, D. 153 Sensabaugh, G.F. 33
DeLeo,D. 92 Kuchheuser, W. 153 Signer,E. 3
Dimo-Simonin, N. 141 Lincoln, P J. 147 Staak, M. 96, 175
Doerfler, W. 50 Lindblom, B. 116 Syndercombe Court, D.
Epplen, J.T. 192 Ludes, B. 187 147
Fabbro, D. 134 MacLeod, A. 3 Szibor, R. 153
Fattorini, P. 134 Mangin, P. 187 Thompson, I.A. 147
Florian, F. 134 Marigo, M. 92 Trabetti, E. 92
Frisan, T. 134 MatM,J. 170 Trommler, K. 221
Fukshansky, N. 203, Monckton, D. 3 TrOger, H.D. 128
208,212 Morris, I.W. 199 Urmetz, S. 79
Gasparini, P. 92 Neumann, R. 3 Veit,A. 85
Geserick, G. 157 Ohshima, T. 175 Watts, P.H. 147
Gibbs,M. 3 Patel, I. 3 Weisser, R.J. 181
Giusti, A.M. 71 Pflugshaupt, R. 160 Wiegand, P. 41,121
Gray,I.C. 3 Phillips, C.P. 147 Wolfes, R. 170
Graziosi, G. 134 Pignatti, P.F. 92 Wrobel, G. 192
Gujer, H.-R. 141 Poche, H. 192 Ziegler, U. 221
Haas, R. 96 Prinz, M. 96,175
Henke, J. 165 Prokop, O. 157
Henke, L. 165 Poers, C. 109
Review Lectures
