DIY FLUORESCENCE EFFECT PART ONE. Alejandro Ariel García Arriaga, Coacalco de ...

13 Pages·2015·2.95 MB·English

DIY FLUORESCENCE EFFECT PART ONE.
Alejandro Ariel García Arriaga, Coacalco de Berriozábal, Estado de México, México.
 INTRODUCTION:  That an object emits or seems to
emit light against a dark background
 For people who love microscopy
takes our mind to fluorescence,
having a microscope of every kind
which is a kind of illumination form
would be a dream, and if one of
that is used within the macroscopic
those microscopes is an electron one
world but also the microscopic one.
the dream would come true, since it
In the latter case there are two
would be possible to magnify things
microscopes that do that function as
up to about a million times. Imagine
both fluorescence and ultraviolet
that power at hand, but most of the
microscopes. For that purpose they
times it is impossible because the
use ultraviolet light or also called
majority of us cannot afford such
black light and fluorescent stains are
things, not even for cheaper
used most of the times as
applications such as confocal,
biomarkers.
ultraviolet or fluorescence
microscopy, they are expensive.  Today I am going to present an
observation that I did with my
 But one of the features that
smartphone’s camera and with my
characterizes the enthusiast
microscope camera and its
microscopist is that we create things
application to microscopy which I
that resemble at least partially those
have called DIY-FLUORESCENCE. I am
applications that we lack. We love
going to divide this topic into several
observation, since we love science,
parts that I am going to expose in
and we try to give application of
several articles because there are
what we observe to this beautiful
several ways of doing DIY-
activity of microscopy.
FLUORESCENCE with the camera
effects, not only with the negative
 DEVELOPMENT:
one and also by using some stains
 Several digital cameras or the ones that are easy to find everywhere.
from smartphones have a marvelous
 NEGATIVE EFFECT OF CAMERAS.
function that is called “negative
effect” which is a marvelous
 I start by presenting what are the
application that allows you to change
changes with the negative effect if a
the image into a kind of darkfield in
camera takes one photo of my office,
which colors also change and some
and is turned into the negative effect
of them change in a beautiful way
using the smartphone’s features, so
that resembles that they are emitting
that an appreciation of what the
light.
negative effect of a camera does can
be seen.



 As it is appreciated in the photos colors turn to a negative look like
above some objects seem to emit this:
light against a darker background so

 ↔ , ↔ , ↔ , ↔ , ↔ , ↔

 As it is evident, purple, blue, red, changing the illumination with the
violet are the most important colors negative effect of the camera,
that gives this effect for that reason I obviously for the ones that change
consider they are the most important with such an effect. In case the
to create DIY-FLUORESCENCE. camera does feature a negative
effect, just take the photo with the
 CREATING THE DIY –FLUORESCENCE
brightfield condenser without filters
and by using the computer program
 The simplest way of creating this
Paint you can select the image and
effect is by placing a sample on the
then turn into a negative for doing
stage of the microscope and
DIY- FLUORESCENCE.

 RESULTS: others converted into negative effect
by using Paint. As you can see there
 Here are some examples of pictures
is no difference with the fluorescent
both taken directly with the negative
effect.
effect of my microscope camera and



 MOSQUITO 4x 20% zoom 10mp

 Mosquito 10X zoom 5mp

 Mosquito´s wing 10x 50% zoom

 MOSQUITO´S ANTENNA 10x 50% zoom

 SPIDER 4x 10% ZOOM


 erythrocytes 40x 33% zoom


 Poinsettia 40x 33% zoom


 Pollen of a yellow lily upon a petal 10x 20%zoom

 Spiderweb and dust found on a wall 10x 25%


 Spider legs 4x 20% zoom

 Salt crystal 10x 20% zoom



 Sugar crystals 10x 20% zoom
 NOTE : photos were processed with the auto
correction of Microsoft Office Picture
The pictures presented below were
Manager. When applied it seem as if
taken in exactly the same way as the
you “turn on the light” in the picture
previous ones, the only difference
and it seemed to me that these
was that in order to get more
photos needed that kind of help to
contrast and the DIY-FLUORESCENCE
get better.
effect could be seen better, these


 Diatoms and green algae 40x 100% zoom

 Mouth epithelial cells 40x 33% zoom
