Development and Validation of Analytical Methodologies for the Determination of Antibiotics in ...

281 Pages·2015·3.14 MB·English

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Andreia Alexandra Ribeiro Freitas

Development and Validation of Analytical
Methodologies for the Determination of Antibiotics
in Food of Animal Origin for Human Consumption

Tese de Doutoramento em Ciências Farmacêuticas, na especialidade de Bromatologia e Hidrologia, orientada pelo Professor
Doutor Fernando Jorge dos Ramos apresentada à Faculdade de Farmácia da Universidade de Coimbra

Maio de 2015

Development and Validation of Analytical
Methodologies for the Determination of Antibiotics
in Food of Animal Origin for Human Consumption

Dissertação do Doutoramento em Ciências
Farmacêuticas, na especialidade de Bromatologia e
Hidrologia, orientada pelo Professor Doutor
Fernando Jorge dos Ramos apresentada à
Universidade de Coimbra

Andreia Alexandra Ribeiro Freitas
Universidade de Coimbra
2015

This work was funded by FEDER through the Operational Program for
Competitiveness Factors – COMPETE, through FCT – Portuguese Foundation for
Science and Technology under the project PTDC/AGR-ALI/122119/2010.

This thesis is based on the following manuscripts:
Freitas, A.; Leston, S.; Barbosa, J.; Ramos, F. Liquid-Chromatography:
Review on the last developments on the detection of antibiotics in food-producing
animals, Liquid Chromatography – Principles, Technology and applications (pp. 99-
139), ISBN 9781626187399, 1ª ed. (2013) New York: Nova Science Publishers Inc.,
USA.
Freitas, A.; Barbosa, J.; Ramos, F. Development and validation of a multi-
residue and multiclass ultra-high-pressure liquid chromatography-tandem mass
spectrometry screening of antibiotics in milk, International Dairy Journal, 33:1
(2013) 38–43.
Freitas, A.; Leston, S.; Rosa, J.; Castilho, M. C.; Barbosa, J.; Rema, P.; Pardal,
M. A.; Ramos, F. Multi-residue and multi-class determination of antibiotics in
gilthead sea bream (Sparus aurata) by ultra high-performance liquid
chromatography-tandem mass spectrometry, Food Additives & Contaminants:
Part A, 31:5 (2014) 817–826.
Freitas, A.; Barbosa, J.; Ramos, F. Multi-residue and multi-class method for
the determination of antibiotics in bovine muscle by ultra-high-performance liquid
chromatography tandem mass spectrometry, Meat Science 98 (2014) 58–64.
Freitas, A.; Barbosa, J.; Ramos, F. Multidetection of antibiotics in liver
tissue by Ultra-High-Pressure-Liquid-Chromatography tandem Mass
Spectrometry, Journal of Chromatography B, 976-977 (2015) 49-54.
Freitas, A.; Barbosa, J.; Ramos, F. Matrix effects in UHPLC-MS/MS
antibiotics multi-detection methods in food products from animal origin, Food
Analytical Methods, accepted for publication on 2015, April, 6th.

CONTENTS
LIST OF FIGURES 13
LIST OF TABLES 15
ABBREVIATIONS 17
ABSTRACT 19
RESUMO 23
INTRODUCTION 27
Antibiotics in Food-Producing animals 27
European Regulation 31
Analytical strategies 35
Validation of analytical methods 41
THESIS OUTLINE 47
REFERENCES 48
CHAPTER I – LIQUID-CHROMATOGRAPHY: REVIEW ON THE LAST
DEVELOPMENTS ON THE DETECTION OF ANTIBIOTICS IN FOOD-PRODUCING
ANIMALS 55
Abstract 56
Introduction 58
Antibiotics and Analytical Strategies 60
Aminoglycosides 65
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