Dendrobium suzukii (Orchidaceae) -A New Species from Vietnam

6 Pages·2002·1.7 MB·English

by  Yukawa, Tomohisa

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ISSN1346 7i6.fi'. ,XctiL Ph)totax .Gcobot. 53{l), 11-16 c2002)
Dendrobium suz"kii(Orchidaceae) - ･A New Species frorn
Vietnam

TOMOHISA YUKAWA
11s'tikuhBaf)tanicaTGaiTl('n,･N'atioiSrcailt"nce,VILsct71tM,.･;mciTAsu'lt)ttAJ'.t3t0h.ai,-OOOS.,1}v)an

A new orchid spccics. Denchvhiit msitJukiiii'ukawa, is dcscribc dfrom Victnarn, I tis easily dis-
tinguishcd froi nlhe closcly rclated Dendtvhittnn'ruenr uRtcnhb. f .by lhe fo11o-'i ncogrnbina-
tion ofeharaetcrsi 1 ) much Iarge prcriant Ihobes ;; ) a semlglobosc callus on the basa lpar tof the
label]Lim ;3) fiv ekee] son the [ubel]un 4i); undul"ie margins on the mid lobe eflhe labellur Sn);
a wider callus wiTh cristute protuherance osn lhe mid lobe of' the Iabcllum, Nlolccular phy]o-
gcncti eanalyscs of' the ITS Cintci ntrnnntscribcd hpacer) region et' thc 1gS-26S nuclear ribosoinu]
DN.,X aiso support a separate taxonemic status efthc ne", spccics und it ssister greLip rclaliens]iip
to D. c'i'uentuni.
Key words/ Dentb'ohiu nsiu:itkii, ITS, molecular phylogcny, ncw species, Orchidaceac ,rux-
onomy. Vietnam
1)enchvhiu mS", .section i:})rmos a(eBcnth. & Materials and Methods
Hook. fl )IIook .f .is a herticultura lilmypor-
tant group to Nvhich about 40 spccies are cur- Materials used in the phylogenct iaciial.vses arc
'l'able
rently assigried, Speciet ibclongi ntog this scetion shosvn in 1 , I)entb'ohiu Jeni'iic'ntuin Rchb.
are tbund from the Himalaya in the west to thc f ,and I) ,tohaen,s'c 'J. J. N}v'o o&d J. B. Comber
PhiIippines and Bernee in the east. Section Nvere included in the study bccause these t"'o
fi?)i'nro sias eusually d¢fined by thc black hairs species show close relatienships to the new
on the leafblad easnd leafsheath Ns{.ost specics species on the basi sof synapomorphic mor-
from the Philippin easnd Borneo. howcvcr ,de phologica lcharacters such as fiowe rcoleur,
not exhibit this featur cIn. fac to,nl.v the hard-tcx- shapc of the sidc Iobes of the label]um, and
tured fio"'e rssi,'it hwhitc, greenish or ycllow shapc of the mentum. A singl ¢ morc clistantly
pcrianth lobes characterize the section. related species in section fl)i'nros ac1) ..formo-
Ph>']ogcnc tainaclyses efmacromolecular char- sttm, was chosen as thc outgroup tuxon. XTeuchcr
acters hnx'c indicate dthat this section does not specimens are dcposited at TrxiS.
represent a monophyletic group ("rongsa"rad Fxperimental methods va'cre described in
et aL 2001. St'ukawa 200 1, unptiblished). .t {ne"' Ef'ukawa ut al. U993, l996). Scqucnces were
spccics froT Tiiv'ietnam, helonging to section detennine dby amp]ifying the ITS (intern tarlan-
RJrniosae ,is describe btcllo" 'I.n this stud>' mol- scribcd spacer) region ofthe 18S-26S nuclear
ccular phylogcnctic analyses were uiso used te ribosomal DNA N'ia the polytneras cchain reac-
clarify thc phylogenetic status of the ncw tion (PCR )from a total DNA cxtract. AII ofthe
species, prirncr swcrc from Douzcry et aL ( 1999) .DNtX
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T,"31.F I. TaNa uscd ibrITg sequencins,.
}'otLfhEI
'SDpe.ne.cgiireos h.i..u.i n .. .. '' .
1'
Rchb. TBC;IS4.,-12*
(v'ttL'ntttm
D('tftlr'vb libutrntntj RNotxtb,n tex I.incfl. TR(;liJO:･4J
I)('n[1ivbium,vuJ"kiiN'ukuxva T .S(i tl. tJ])
Penc/robiui rn"haE,nke J, J. .Y.'o oSd! .J. B.CL)mhcr TB(TtttJ6SJ
+'T'nG1's usekruicbsa hidicn tncecegsion nu]nh ¢rg e f t he l lL iLn gcol[ection datubuh eal
Botanica ]Garden.
sequences were a]igncd manua]ly. Gaps wcre Plunt to 27 cin tal] .Stenis canc-like. clus-
treate das missing characters. The aligned data tcred, erect, narrowly fiisiform ,slightly Hat-
fi] cis ayailable freni the author upon request. tencd, weakly sulcatc in age. Ieafy throughout,
Parsimony and distanc eanalyses wcre con- tightl> 'coycred by persistet iltea fsheaths, te 23
ducted with PAUP' version 4.0b6 (Swofford cm long ,O.8 cm in diameter ,Leaves patent,
2001 ) .The branch and bound scarch option was coriaceous, glossy ,oblong-lanceolate, sessile,
used to pcrform Fitch parsimony ana]yses (Fitchapex uncqually bilobed ,dark green, both sur-
1971). Branch length sfor trccs vv'ere calculated face s"ith dense blackis hhuir s2,. 1-7, 3cm long,
using the ACCTR.ALN optimization {.gwofYl )&rd O.g-2.2 cTn "'idei sheaths clasping, thin, dull
ry4addiso n19g7). Distance trees were obtained grccn "'ith dengc blackis hhairs ,O.3-3, 2cm
using the neighbor-joining mcthod (Saito u& long .Inflorescen csuebercct-putent, axillary on
Nci I987) with a Kimura t",o-paratneter cor- apical halfof fl)Iia tsteenis. bearing 1 or 2 flow-
rectien {Kimura 1980) .To assess thc relative ers successivel>'; pedune]e inconspicuo uesnt,ire-
robustness ft) brrancheg ,the boetstrap Tncthod ]y enclosed by bract s4, mm long; rachis 4 mm
(Fclsenstc i1n985) was used "ith 10000 repli- ]ong; tlora lbraet sobliquely lanccolat aecu.mi-
cates. nate, eoncave, brownish, abaxial surface wiTh
dense brown hairs a,daxial surfuce glabreus, to
Resultsand Discussion 14.5 mm Iong, 4.L tmsmF wide. Flowers widely
opening, gloss> ;thick, rigid; sepals and pctals
l)escJ'ipt qifo'nth e}tat' speeies paic ycllowish green ,veins darkcr: labellum
Dendrobium suz"kii YtLka-'a ,sp.nos,. pale yellowish grcen ,sidc lobes and thickened
arcas sanguine; column and anthcr cap pale
AMne 1)endJ'ohi ocruento Rchb, tl .scd grccn; column foo tpale green stained sanguine;
fleribu svaldc ma.joribus, disco labcl lqiuinque- pellini aorangish yellow ;pedieellat ocvary grass
carinato et cristato, callo Iabel lgcimiglobosus, ct grccn, Dorsal sepal lanceolat cac,utc, mucro-
marginibus labcll iundulates distinguendu ,rn nate, 35-40 rnm long ,14,)'- 1r6nm wide. LateraL
Ti7 )VeIETTN',rXM. Ninh Thuin Provinc ci,n sepals obliquellr triangular-lanccolate, acute.
the vicinity ofNinh Son. Flo"'erin gin cultivation mucronale, abaxial surface costate, 39-40 mm
,ALugust 2001, Itort .71 Sttt og. n. (IIolotypIeon:g along antcrior margin, 19-2l mm wide at
TNS). Figs ,1, 2. base; mentum obtuse, making a right tiliglc -'ith
Pttraov)e :VIETNAM. Lfim Dong Prov- ovary, 16-17,5 mm long .Petal slinear-lanceolate,
ince. alt. ca. 1000 m. S. Krh,'ttef tKe!i''-dOOII ncutc, 31-33 mni long. 7.5-9 mm widc.
(.TNS}, I.abellum threc-lobed, shortly clawed, five-
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Fl(},] ,Flo"'er s"fDetitbvhiuJn yu:ukii Yuka-'a (tX- aJru){ I)eJttirohi cuii'intentttnT Rchb, f .CK, L). ,A LF,Iv",cr f,ron tNic"'; B.
Po[linium C;. ,SLnth cenpr; D. Column and column foet f,rom be]ow ;E. I.ubc]]u mand column, side vic"･; E Dorsa] sepal:
G. Peta] ;I{ .I.nler nslcpul; [ .Labe[luni j.. Closcup ofcallus on [niLl iebe efIabellum; K. I.'abelluT "1.; ,Closeup efcul-
lus on mid lobe uf' labcllum .Drawn from holol} ,cp7e T,SIi st. tti,.) by lvt N.aknjima. Sca[e bar 1 cm (A) ).' mm CD-I ,K).
3. CB.C}.
or Tnm
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FT{,2 F]oivenng indi! idLial ofPeitcbT)htttn! yrt:ttktt }'ukazvaPhoto.graph from holot}pe CT Sat(, y n )
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June ]OO! YUKAW,NlNc", D{･tith'"hiun tfromVietnam ]).
kccled betwcen sidc lobcs ,with a semigloboge instea dofvernicosc, protuberances on the mid
ca]lus at base ,33 mrn long. 28 mm wide; side lobe ofthe labellum.
lobes erect, falca¢t, obtusc, IO-12 mm Iong, D('ndivbitt m,rti:tt k"Jiais ft)un dniore than
7,5-9 mm wide; mid lobe obovate, obtLLse, undu- 1000 km from the central part of the Malay
late ,apical part of adaxial surface papillose, Peninsula "'here D. ertientutn Nvas collcctcd
(SeidenfadIe9n85).The
rugose, with a cnstatc callus at centre ofadaxi- other closely rclated
a] surface, abaxia] surface shallowly sulcate, spccies, D, tobaense is known only ft'o Ttnhe
21.5-2 2mm long ,li-18 mm wide. Colllmn northern Par otfSumatra, Dentbvhitit nsu:ttAii is
dilate dat base ,5 mm long ,6,5 mm wide at gcographicall yisolal efdrom those closely relat-
base; stelidia short, triangular, obtusc; colunin ed spccies,
foo tslightly cun'ed, basall ygroovcd ,1 1 mm Nine spccies of Dendiobium section 1･br-
long ;anther cap cucullate. quadratc, hirsutc mosae haN, beeen recorded fiDm Xtrictn a(mSeiden-
belo" '3,.8 rnm ]ong ,3.8 mm ",ide; pollini a4, in faden 1992), but D. su"tkii ig easily discrinii-
2 pairs ,orangish ycllow ,3 mm long .Pedicellatenated froT nthem b] ff'lowe rcolour and strueturc
ovar}' narrowly clavate, weakl>' sulcate, g]a- ofthe cal]us on the ]abelluni.
breus, 2 ･3 mm long.
Eu'tnologiT'hie honours Piu'logenetic
spccifie cpithet anabts'as
Kam]hiro Suzuki ofT'sukuba Botanical Gardcn, Ninc nucleotide positio nwesre potentia lilnyfor-
who noticcd the distinctiven eefsthsis species. mative out uf9I7 bp ofthe l8S (i npart)-I T1S-
5.8S-IT S2-26S (i npart )sequcnces deteimiined.
Dendtvhium sti:ukii is most siniilar to D, The parsimony ana[ysis resulted in a singlc most
cruentttm but can be distinguishe dby the fo1- parslmonious tree of 78 steps (Fig .3) that
lowing charactersi 1) much ]arge rperian tlhobes; show'cd a sister group relationship between
1)a serniglobose ca]lus on thc basa lpart ofthc Dendtvbitm Tsu7tikii and D, ('ruenttun. The trec
labcllum; 3J five ,instca dofthree, keel son the had a consistency index {CI )ofO,9359 (O.6 ,9i2･
labellu m4;) Lmdulate margins on the mid lobe of excluding unin formati vceharactcrs) and a reten-
the labeHum; 5) a widcr callus "ith cristate, tion index (RI )ofO.4sl･44. The neighborjoining
P,suzuk"
nturn
ib.tobaense
D.formosurn

F[c, 3.. Singl emo]t parsimoniouh Fitch tree bnsed upon ITS hequences/ length 7g, ¢onsisrenc>･ index O.935g (O.(Yl e2xc9lud-
ing uninf'orn)ative charucters}, retenCion index ofO,4444. NunihcrK abnvc internode sinJicnt neuc[eetide liubstilulions
supporting ench monephyletic group ( ,liLCCTR,hLN uptimizati L}n).
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topologlr '"ras identica l(rcsu lntots sho"'n). The evo]utionar>' ratcs ot' basc substitutiuns Through
bootstra panalyses rnedcrate]y supported t]ic comparatiyc htudies ot' nuc]cvtide scquenccs, J,
sister group rclationship bctween D, ,yu:itk ianid N{o] flvol .16, [11-120.
D. eJ'iientitin {58e' osupport ft) rthe parsimony S a i tmoctu h,oId/I. & aN n{c. "'Nc imc.t he[d9S7 . foTrh cre connesitgnhtbcotri-njgoimng ph>'-
aannaa]]yyssiiss,). 4TCh)c[ } sn'uubpmpboerrt fofor tbhacs ne cdigihfbofrjborineinngc eansd Sc i d elnofgucdnccnt,i c Glr, ee[s9. gN5･.I o0] rB.ciholi. d EgKcon]c, r4a/ 4i0n 6'-V4h25a.i]and
scqucncc divcrgencc s"ithin thc ]TS region XII, Dcndrobiun ]Sw. ()pera Botanic ag3/ 1-
",ere as ft)Ilo" 'sDi. yu:tikii-I), fbrniost i3gn,n 2 9.5..
O.081O ;l) .su:ukii-D, (･tutc,ntum: 2g, O.0592: I), 1992. The orchids ot' Indechlna O.pcra Botanica
II4i]-502,
stun:oulkeiciu-lDa.r tchoalr)aaccte'r]sn,, s3 'D8e.i, Ost,i0ru8A0'i3i .Bias dsicsd teinn tcfhtrcosern S s v ouisTinogr d Dp,.a rIs.i .m2o0n0y1 . cP*iaXnId' Po't h/eprh} 'mcltche,dgs)c,ne¢t i avncarsliyosnis
by its base
close]y rclated specics uniquc sub- 4.()b6 .ginaucr ,Sundertand, Nlassachusctts L,'SA,
stitutions in the ITS region and appears to have & "". P. TvTnddison .19S7. Reconstrueting anccstra]
a sister group relationship ",ith l) ,c'ru(Jntuni, character states under "･'abTn eprarsimony. TL'Iath,
Biosei. R7/ 199-229,
NVengsawad, P.,T. IIanda & T, Er'ukawa, [IO{)l,
1 would Iike te thank Takee Suie and SLibhkon
Khruekcrd for prox'idin gthc p]an tniaterial, Osatnu l.･Teleeulur phylogcn} 'of DE,mli'"hi itsemction
Dendrohium. In/Nagata,II,& S,Ichihashi,
)v{iikc dPah.cravu tXiv･'ongsawa dan,d IIideak iShimi7u
R}r lechnicul LLssistttncc. itnd rvTutsuk Noakajima for teds.) Proc. 7 .,SL sPi,aLcif iOcrchid ('on[ l)p .209-
preparin gthe illustratiens. 2 1O. Secretari osfl sXPOC7, ('hiry uJ,apan,
1'ukawa. T..S. KL]rita,Nl.Nishidu & }･T.IIuscbc.

Rererences 19C)3 .Ph.vlogencti eimplicatio nest' chleroplast
DN,X restrietion sitc variation in subtribc
Dendrobiina ecOrchidaceae). I ind[uyan aR/ 2 1 I-
Douzery, E' .J. P., ,X. M. Pridgeon. P. Kores. H. P, 22l.
I.inde rl,l .Kur7xyci [& M. "'. ('hasc, 1999, 2001. },ffolecLt lpahr>,logeny ot' Dendt'ohium. bi/
),Io]ecul aprhylogcnetics of Discac (Orchida- Nagata, H. & g. Ichihnshi .(eds. )Proc. 7 ,Xsia
ceac)/ tX contribution fron ]nue]ear mbosoni"[ Pucific Orchid Cont' .pp, 69-71. Sccretari osft
ITS scqucnccs, ,Xmen J ,Bot. g6/ S87-SL)[), ,XPOC'7, ('hirl rJ,aup,an.
J'e]scnstc iJn .,1 C)S5. Confidcnc climit sen phy]ogc- .rm ,II ,()hba ,K. X･ T(,':n]ero &n Nl. "', C'hase .1 L)`)6.
nies/ an approncli using the beetstra pI."x'elutien Chloroplas[ I)ts' Aph)logcny of subtribc
3() I7S3-79 ] , 'I'o",ard Dendrobiinae COrchidnccae )i/nsights from a
Filch. VN' ZM. 197 '1. def}ning rhe course ol' combined analysis based on i'1]{'1. sequcnces and
cyolution/ minimum ehangc ror a speciilc tree restriction site var{{Ltion. J. PIan tRes, 109/ 169-
topelogy, Sygl, Zoel. 2e: 406-416. I76.
Kitnura ,Nl ,i980. ,X siniple inethod t'e restin];Lting
Re('eii'eti,lan7t.i2a0i0)2'uJc'('c7)Atftt'"['tc2'Sh,20t)2

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