Table Of ContentCYTOKERATINS –
TOOLS IN ONCOLOGY
Edited by Gerhard Hamilton
Cytokeratins – Tools in Oncology
Edited by Gerhard Hamilton
Published by InTech
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Copyright © 2012 InTech
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First published February, 2012
Printed in Croatia
A free online edition of this book is available at www.intechopen.com
Additional hard copies can be obtained from [email protected]
Cytokeratins – Tools in Oncology, Edited by Gerhard Hamilton
p. cm.
ISBN 978-953-51-0047-8
Contents
Preface IX
Part 1 Expression of Cytokeratins in Nonmalignant Tissue 1
Chapter 1 The Expression of Cytokeratins in Bovine
Intestinal Microfold (M) Cells 3
Takashi Kanaya, Tetsuya Hondo, Kohtaro Miyazawa,
Michael T. Rose and Hisashi Aso
Chapter 2 Cytokeratins of the Liver and Intestine Epithelial
Cells During Development and Disease 15
Priti Chougule and Suchitra Sumitran-Holgersson
Part 2 Expression of Cytokeratins in Malignant Tissues 33
Chapter 3 Epithelial to Mesenchymal Transition in
Microbial Pathogenesis 35
Abderrahman Chargui, Mimouna Sanda,
Patrick Brest, Paul Hofman and Vouret-Craviari Valérie
Chapter 4 Cytokeratin 7 and 20 55
Agnieszka Jasik
Chapter 5 Cytokeratin 8: The Dominant Type II
Intermediate Filament Protein in Lung Cancer 67
Nobuhiro Kanaji, Akihito Kubo, Shuji Bandoh, Tomoya Ishii,
Jiro Fujita, Takuya Matsunaga and Etsuro Yamaguchi
Part 3 Cytokeratins as Markers of Tumor
Dissemination and Response 97
Chapter 6 Cytokeratin 18 (CK18) and CK18
Fragments for Detection of Minimal
Residual Disease in Colon Cancer Patients 99
Ulrike Olszewski-Hamilton, Veronika Buxhofer-Ausch,
Christoph Ausch and Gerhard Hamilton
VI Contents
Chapter 7 FISH Probe Counting in Circulating Tumor Cells 119
Sjoerd T. Ligthart, Joost F. Swennenhuis,
Jan Greve and Leon W.M.M. Terstappen
Chapter 8 Cytokeratin 18 (CK18) and Caspase-Cleaved CK18 (ccCK18)
as Response Markers in Anticancer Therapy 135
Hamilton Gerhard
Preface
The present volume “Cytokeratins – Tools in Oncology” is a new addition to the
Intech collection of books that aims at providing scientists and clinicians with a
comprehensive overview of specific aspects of the latest research and current
knowledge related to the application of cytokeratins in histology, cancer research and
clinical diagnosis of tumors. For this purpose a series of research articles, clinical
investigations and reviews that deal with the role of the most abundant cytokeratin
types in normal and malignant tissues were included. This volume aptly adds to the
other Intech titles in the field of oncology, particularly describing advances in cancer
therapy, diagnosis and treatment of individual tumor entities as well as the important
topic of cancer stem cells.
The participating authors shared their expertise about the multiple functions of
cytokeratins in organization of the intermediary filaments in normal intestine and liver
as well as microfold L cells and, furthermore, the usability of cytokeratins 7, 8, and 20
in tumor diagnosis. Epithelial to mesenchymal transition as a mechanism important in
pathogenesis is touched in another chapter, followed by several articles discussing the
assessment of cytokeratins in disseminated tumor cells and as response markers
during chemotherapy. Our group published reports on the use of cytokeratin
fragments in prostate cancer patients undergoing intermittent androgen suppression
in the late nineties and it was fascinating to observe the continuative development of
anti-cytokeratin reagents as diagnostic and possible predictive tools available in the
form of refined assays providing highly valuable means to detect the presence of
disseminated tumor cells and minimal residual disease in cancer patients. This book is
therefore destined to all cancer researchers and therapists who want to understand the
diagnostic use of cytokeratins in histology and especially to tackle the challenge of
finding residual tumor cells in patients, either circulating in the blood or residing
hidden in niches, that may lead to tumor recurrence and qualify patients for a more
aggressive anticancer therapy.
As editor of this book, I would like to acknowledge the significant efforts made by all
of the contributing authors and the entire editorial team in publishing of this work. I
would like to dedicate this book to the “Ludwig Boltzmann Society” and, in particular,
to Prof. Dr. Gerhard Baumgartner whose long-standing support has allowed for the
X Preface
successful realization of many scientific projects. Last but not least, I would like to
thank my wife for her personal support and great patience at all times.
Gerhard Hamilton, PhD
Ludwig Boltzmann Cluster of Translational Oncology, Vienna,
Austria
