Table Of ContentHee-Jong Koh · Suk-Yoon Kwon
Michael Thomson Editors
Current
Technologies in
Plant Molecular
Breeding
A Guide Book of Plant Molecular
Breeding for Researchers
C urrent Technologies in Plant Molecular Breeding
H ee-Jong K oh • S uk-Yoon K won
M ichael T homson
E ditors
C urrent Technologies in
Plant Molecular Breeding
A Guide Book of Plant Molecular
Breeding for Researchers
E ditors
H ee-Jong K oh S uk-Yoon K won
C ollege of Agriculture and Life Sciences P lant Systems Engineering
Crop Science and Biotechnology Research Center
S eoul National University K RIBB, D aejeon, K orea, Republic of
S eoul, K orea, Republic of (South Korea) (South Korea)
M ichael T homson
P lant Breeding, Genetics,
and Biotechnology
I nternational Rice Research Institute
L aguna, P hilippines
I SBN 978-94-017-9995-9 ISBN 978-94-017-9996-6 (eBook)
D OI 10.1007/978-94-017-9996-6
L ibrary of Congress Control Number: 2015946612
S pringer Dordrecht Heidelberg New York London
© Springer Science+Business Media Dordrecht 2 015
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P refa ce
P lant breeding, the science for plant genetic improvement, made great progress in
the twentieth century with the rediscovery of Mendelian genetic principles in 1900.
Most of the traditional breeding methods were established before the 1960s leading
to the development of high-yielding varieties in cereal crops which brought the
“Green Revolution” during the 1960s–1980s. Recent progress in biotechnology and
genomics has expanded the breeders’ horizon providing a molecular platform on the
traditional plant breeding, which is now known as “plant molecular breeding.”
Under a new paradigm of plant breeding in the twenty-fi rst century, breeders try to
create new variation through the direct manipulation of target genes instead of
phenotype-b ased trait selection. Genetic resources are extended to the unrelated
species because transgenic technologies break through the sexual limit for gene
transfer. In addition, selection and genetic fi xation in the progeny can be performed
by monitoring of genes and genomics information by which breeders can develop
new varieties precisely and quickly.
Although diverse technologies for molecular breeding have been developed and
applied individually for plant genetic improvement, the common use in routine
breeding programs seems to be limited probably due to the complexity and incom-
plete understanding of the technologies. This book is intended to provide a guide for
researchers or graduate students involved in plant molecular breeding by describing
principles and application of recently developed technologies with actual case stud-
ies for practical use.
T his book is organized in nine chapters. In Chap. 1 , a brief history and perspec-
tives of plant breeding are presented, including the directions of future development
of breeding methods. In Chap. 2 , the basics on genetic analysis of agronomic traits
are described, including how to construct molecular maps and how to develop DNA
markers. In Chap. 3 , methods of detecting QTLs are illustrated, while in Chap. 4 ,
the application of molecular markers in actual plant breeding is described in detail
with case studies. In Chap. 5 , genome sequencing and how to analyze the associa-
tion between sequencing data and phenotype are introduced, including the epig-
enome and its possible application to plant breeding. In Chap. 6 , genome-wide
association studies are explained so that researchers can analyze the data following
v
vi Preface
the manual including the introduction of software for analysis of population
structure. In Chap. 7 , methods for mutation screening and targeted mutagenesis are
described. In Chap. 8 , how to isolate the genes of interest and how to analyze the
gene function are presented with case studies. In Chap. 9 , the basics of gene transfer
in major crops and the procedures for commercialization of GM crops are explained.
We attempted to cover most of the molecular tools applicable in plant breeding;
however, due to the limitation of the book volume, we had to skip some skills that
are still under development. Therefore, in this book, only key technologies which
are currently used in plant breeding are mentioned. Since technologies per se are
being advanced, we may add newly emerging ones with a chance given later. We
hope this book would be a valuable reference for plant molecular breeders and, in
addition, will become a cornerstone for the development of new technologies in
plant molecular breeding for the future.
W e are indebted to all the authors for their dedicated efforts and their time in
writing the chapters despite the busy schedule. We are greatly thankful to Springer
Publishing Co., Editorial Team, and particularly to Ms. Sophie Lim of Springer
Korea for her support during the process of preparation and editing of the manu-
scripts. Our thanks extend to Dr. Mi-ok Woo for her clerical assistance.
S eoul, R epublic of Korea H ee-Jong K oh
D aejeon, R epublic of Korea S uk-Yoon K won
M etro Manila, P hilippines M ichael T homson
C ontents
1 B rief History and Perspectives on Plant Breeding ................................. 1
J oohyun L ee, J oong H youn C hin, S ang N ag A hn, and H ee-Jong K oh
2 M ethods for Developing Molecular Markers ......................................... 15
H ee-Bum Y ang, W on-Hee K ang, S eok-Hyeon N ahm,
and B young-Cheorl K ang
3 Q TL Identification .................................................................................... 51
H yun S ook L ee, S un-Goo H wang, C heol S eong J ang,
and S ang N ag A hn
4 M arker-Assisted Breeding ........................................................................ 95
J ae B ok Y oon, S oon-Wook K won, T ae-Ho H am, S unggil K im,
M ichael T homson, S herry L ou H echanova, K shirod K . J ena,
and Y ounghoon P ark
5 G enomics-Assisted Breeding .................................................................... 145
I k-Young C hoi, H o-Jun J oh, G ibum Y i, J in H oe H uh, and T ae-Jin Y ang
6 C oncept of Genome-Wide Association Studies ....................................... 175
C hang-Yong L ee, T ae-Sung K im, S anghyeob L ee, and Y ong-Jin P ark
7 I dentification of Mutagenized Plant Populations ................................... 205
G eung-Joo L ee, D ong-Gwan K im, S oon-Jae K won,
H ong-Il C hoi, and D ong S ub K im
8 I solation and Functional Studies of Genes .............................................. 241
M i-Ok W oo, K esavan M arkkandan, N am-Chon P aek,
S oon-Chun J eong, S ang-Bong C hoi, and H ak S oo S eo
9 P lant Transformation Methods and Applications .................................. 297
Y oung H ee J oung, P il-Son C hoi, S uk-Yoon K won, and C hee H ark H arn
I ndex ................................................................................................................. 345
vii
C ontributors
S ang N ag A hn D epartment of Crop Science, C hungnam National University,
D aejeon, R epublic of Korea
J oong H youn C hin I nternational Rice Research Institute (IRRI), M anila,
P hilippines
H o-Jun J oh D epartment of Plant Science, S eoul National University, S eoul,
R epublic of Korea
H ong-Il C hoi Advanced Radiation Technology Institute, Korea Atomic Energy
Research Institute, J eongeup, R epublic of Korea
I k-Young C hoi G reen-Bio Institute of Science and Technology, S eoul National
University, S eoul, R epublic of Korea
P il-Son C hoi O riental Pharmaceutical Development, N ambu University, G wangju,
R epublic of Korea
S ang-Bong C hoi D ivision of Bioscience and Bioinformatics, M yongji University,
Y ongin, R epublic of Korea
T ae-Ho H am D epartment of Agricultural Sciences, K orea National Open
University, S eoul, R epublic of Korea
C hee Hark H arn N ongwoo Bio Co., Y eoju, R epublic of Korea
S herry L ou H echanova Plant Breeding, Genetics and Biotechnology Division ,
I nternational Rice Research Institute, M etro Manila, P hilippines
J in H oe H uh D epartment of Plant Science and Plant Genomics and Breeding
Institute, S eoul National University, S eoul, R epublic of Korea
S un-Goo H wang D epartment of Applied Plant Sciences, K angwon National
University, C huncheon, R epublic of Korea
All of the co-authors equally contributed to this chapter.
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