Cryopreservation of Mammalian Gametes and Embryos: Methods and Protocols

360 Pages·2017·9.9 MB·English

by  Zsolt Peter Nagy

Methods in
Molecular Biology 1568
Zsolt Peter Nagy
Alex C. Varghese
Ashok Agarwal Editors
Cryopreservation of
Mammalian Gametes
and Embryos
Methods and Protocols
M M B
ETHODS IN OLECULAR IOLOGY
SeriesEditor
JohnM.Walker
School of Lifeand MedicalSciences
University of Hertfordshire Hatfield,
Hertfordshire, AL109AB,UK
Forfurther volumes:
http://www.springer.com/series/7651
Cryopreservation of Mammalian
Gametes and Embryos
Methods and Protocols
Edited by
Zsolt Peter Nagy
Reproductive Biology Associates
Atlanta, GA, USA
Alex C. Varghese
ASTRA Fertility Group
Mississauga, ON, Canada
Ashok Agarwal
Cleveland Clinic Foundation
Cleveland, OH, USA
Editors
ZsoltPeterNagy AlexC.Varghese
ReproductiveBiologyAssociates ASTRAFertilityGroup
Atlanta,GA,USA Mississauga,ON,Canada
AshokAgarwal
ClevelandClinicFoundation
Cleveland,OH,USA
ISSN1064-3745 ISSN1940-6029 (electronic)
MethodsinMolecularBiology
ISBN978-1-4939-6826-8 ISBN978-1-4939-6828-2 (eBook)
DOI10.1007/978-1-4939-6828-2
LibraryofCongressControlNumber:2017934237
©SpringerScience+BusinessMediaLLC2017
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Preface
Cryopreservationofoocytes,sperm,andovarianandtesticulartissues,aswellasembryos,is
one ofthemostcritical proceduresto preserve thereproductivecapacityofindividuals. To
grasp its importance it is essential to know that in the United States alone, there are over
forty thousand women younger than 40 years old who develop malignant tumors every
year—many of whom do not have children/families and whose fertility capacity is at risk
becauseofthediseaseor themedicaltherapyappliedagainstthedisease.Theirbestchance
to have children in the future is to cryopreserve their gametes or reproductive tissues/
organs. Medical conditions can also affect adult men and adolescents, where sperm or
testicular tissue cryopreservation servesas thebest optionto maintain their future fertility.
In addition to men and women with medical conditions healthy individuals may also
consider preserving their fertility at a younger age to ensure that when they are ready to
have a family, they would be able to have their biological offspring. It is a well-known fact
thatmostcountriesarefacinganinfertility“epidemic”becausesocialconditionsarechang-
ingandindividualsarepostponingmarriageand/orhavingchildren.Asaresult,over20 %
of women at the age of 45 are childless, mostly involuntarily. In the past, cryopreserving
ovarian tissue and oocytes were a challenge, but today, with recent breakthroughs in
technology, female reproductive cells and tissues can be preserved much more efficiently.
Age-relateddeclineinfemalefertilityiswellestablished;however,thereareindicationsthat
age (ageing) may affect men also. There is an increasing number of studies that show a
correlation between paternal age (above 45) and higher incidence of different disorders,
includingautismandcertainmentalillnesses.For thesereasons,cryopreservationofrepro-
ductivecellsandtissuesisfastbecomingacriticalpartofmodern reproductivemedicine.
Furthermore, it is for these reasons that we aimed to bring a book to readers of the
medical profession that provides a comprehensive and technically detailed presentation on
allaspectsofcryopreservationofreproductivecellsandtissues.Thisbookpresentscurrent,
well-establishedprocedures,aswellasnoveltechniqueswiththelatestinnovationsdescribed
indetail.Thisbookisextremelyvaluablebecauseeachtopiciswrittenbyaworld-renowned
author,whoisaleaderintheirfield.
We want to express our sincere gratitude to all of the authors who helped write these
outstanding chapters for this book. Our special appreciation goes to the development
editor, Mike Koy, for his outstanding support from the beginning to the end. The editors
arealsothankfultotheir familiesfor theirloveandsupportinthisimportantendeavor.
Wearetrulyhonoredtobeabletobringthisbooktoyou.
Atlanta,GA,USA ZsoltPeterNagy
Mississauga,ON,Canada AlexC.Varghese
Cleveland,OH,USA AshokAgarwal
v
Contents
Preface ..................................................................... v
Contributors................................................................. ix
PART I INTRODUCTION
1 HistoricalBackgroundonGameteandEmbryoCryopreservation ............ 3
JaffarAli,NaifH.AlHarbi,andNafisaAli
PART II BASICS AND ADVANCED BIOLOGY
2 UtilityofAnimalModelsforHumanOvarianTissueCryopreservation........ 23
SeulKiKim,JungRyeolLee,andS.SamuelKim
3 CurrentChallengesinImmatureOocyteCryopreservation .................. 33
Ri-ChengChian,YixinXu,andDanaKeilty
4 RoleofAntioxidantsandAntifreezeProteins
inCryopreservation/Vitrification ......................................... 45
SeulKiKim,HyeWonYoum,JungRyeolLee,andChangSukSuh
PART III METHODOLOGY
5 SlowFreezingofHumanSperm.......................................... 67
AshokAgarwalandEvaTvrda
6 TechnologyofAsepticCryoprotectant-FreeVitrification
ofHumanICSISpermatozoa ............................................ 79
VladimirIsachenko,RaulSanchez,PeterMallmann,GoharRahimi,
andEvgeniaIsachenko
7 HumanEpididymalandTesticularSpermCryopreservation.................. 85
PankajTalwarandSarabpreetSingh
8 HumanOocytesSlow-RateFreezing:Methodology......................... 105
C.Zaca` andA.Borini
9 SlowFreezingandThawingofHumanCleavageStageEmbryos ............. 119
DavidH.Edgar,JanellArcher,andDebraA.Gook
10 HumanOocyteVitrification.............................................. 131
LauraRienzi,AnaCobo,andFilippoMariaUbaldi
11 HumanEmbryoVitrification............................................. 141
JuergenLiebermann
12 HumanOvarianTissueSlowFreezing..................................... 161
DebraA.Gook
13 HumanOvarianTissueVitrification....................................... 177
ShermanSilber
vii
viii Contents
14 EstablishinganOocyteCryobankNetwork ................................ 195
JamesGraham,JoshLim,andMichaelTucker
15 DevelopmentofaNationwideNetworkforOvarianTissue
Cryopreservation ....................................................... 205
JanaLiebenthron andMarkusMontag
PART IV ADVANCES IN CRYOTECHNOLOGY, RESEARCH, AND ANIMAL MODELS
16 DirectionalFreezingofOvarianTissueandFreeze-Drying
ofStemCellsforFertilityPreservation .................................... 223
AmirAravandYehuditNatan
17 SterilePlate-BasedVitrificationofAdherentHumanPluripotent
StemCellsandTheirDerivativesUsingtheTWISTMethod................. 231
JuliaC.Neubauer,FrankStracke,andHeikoZimmermann
18 Vitrification:AReliableMethodforCryopreservation
ofAnimalEmbryos ..................................................... 243
B.Singh,G.Mal,andS.K.Singla
19 CryopreservationEffectonGeneticFunction:NeonatalOutcomes........... 251
VanesaRobles,MartaF.Riesco,andDavidG.Valcarce
20 Gavi-AutomatedVitrificationInstrument.................................. 261
TammieK.Roy,SusannaBrandi,andTeijaT.Peura
PART V APPENDICES
®
AppendixA:Cryotech VitrificationThawing .................................. 281
GoralGandhi,MasashigeKuwayama,SakinaKagalwala,
andPriyankaPangerkar
AppendixB:SolidSurfaceVitrification......................................... 297
MohanS.KamathandK.Muthukumar
AppendixC:AutomatedVitrificationofMammalianEmbryos
onaDigitalMicrofluidicDevice............................................... 309
JunLiu,DerekG.Pyne,MohamedAbdelgawad,andYuSun
®
AppendixD:IrvineScientific VitrificationSystem.............................. 317
MatthewVerMilyeaandAmberBrewer
AppendixE:Rapid-iTM:ClosedVitrificationDevicebyVitrolife .................. 335
MarkG.Larman
AppendixF:Quinn’sAdvantageEmbryoFreezeKit............................. 343
KiriBeilbyandPatrickQuinn
AppendixG:VitrificationofBlastocystsUsingVitriBlast™
andThermoBlast™:Nidacon................................................. 355
AnnaNila€ngLaessker,ThorirHardarsson,Ann-SofieForsberg,
TetsunoriMukaida,andPaulV.Holmes
Index ...................................................................... 367
Contributors
MOHAMEDABDELGAWAD (cid:1) DepartmentofMechanicalEngineering,AssiutUniversity,
Assiut,Egypt
ASHOKAGARWAL (cid:1) AmericanCenter forReproductiveMedicine,ClevelandClinic,
Cleveland,OH,USA
NAIFH.ALHARBI (cid:1) REIMDepartment,Women’sSpecializedHospital,KingFahadMedical
City,Riyadh,KingdomofSaudiArabia
NAFISAALI (cid:1) SchoolofSociology,UniversityofNewSouthWales,Sydney,NSW,Australia
JAFFARALI (cid:1) FacultyofMedicine,DepartmentofObstetricsandGynaecology,
UniversityofMalaya,KualaLumpur,Malaysia
AMIRARAV (cid:1) FertilesafeLtd.,NessZiona,Israel
JANELLARCHER (cid:1) ReproductiveServices/MelbourneIVF,RoyalWomen’sHospital,Parkville,
VIC,Australia
KIRIBEILBY (cid:1) MonashUniversity,Melbourne,VIC,Australia
A.BORINI (cid:1) 9.babyFamilyandFertilityCenter,Bologna,Italy
SUSANNABRANDI (cid:1) GeneaBiomedx,Sydney,NSW,Australia
AMBERBREWER (cid:1) ArizonaReproductiveMedicineSpecialists,Phoenix,AZ,USA
RI-CHENGCHIAN (cid:1) DepartmentofObstetricsandGynecology,McGillUniversity,Montreal,
Canada
ANACOBO (cid:1) CryoPreservationLaboratory,IVIValencia,Valencia,Spain
DAVIDH.EDGAR (cid:1) ReproductiveServices/MelbourneIVFandDepartmentofObstetrics
&Gynaecology,RoyalWomen’sHospital,UniversityofMelbourne,Parkville,VIC,
Australia
ANN-SOFIEFORSBERG (cid:1) ProductDevelopment,NidaconInternationalAB,Mo¨lndal,Sweden
GORALGANDHI (cid:1) IVFDepartment,Rotunda—TheCenterForHumanReproduction,
Mumbai,Maharashtra,India
DEBRAA.GOOK (cid:1) ReproductiveServices/MelbourneIVFandDepartmentofObstetrics
&Gynaecology,RoyalWomen’sHospital,UniversityofMelbourne,Parkville,VIC,
Australia
JAMESGRAHAM (cid:1) ShadyGroveFertilityReproductiveScienceCenter,Rockville,MD,USA
THORIR HARDARSSON (cid:1) FertilityCenter,CarlanderskaHospital,Gothenburg,Sweden
PAULV.HOLMES (cid:1) ProductDevelopment,NidaconInternationalAB,Mo¨lndal,Sweden
VLADIMIRISACHENKO (cid:1) DepartmentofObstericsandGynecology,MedicalFaculty,University
MaternalHospital,CologneUniversity,Cologne,Germany
EVGENIAISACHENKO (cid:1) DepartmentofObstericsandGynecology,MedicalFaculty,
UniversityMaternalHospital,CologneUniversity,Cologne,Germany
SAKINAKAGALWALA (cid:1) IVFDepartment,Rotunda—TheCenterForHumanReproduction,
Mumbai,Maharashtra,India
MOHANS.KAMATH (cid:1) ReproductiveMedicineUnit,ChristianMedicalCollege,Vellore,Tamil
Nadu,India
DANAKEILTY (cid:1) DepartmentofObstetricsandGynecology,McGillUniversity,Montreal,
Canada
SEULKIKIM (cid:1) DepartmentofObstetricsandGynecology,SeoulNationalUniversity
BundangHospital,Seongnam,SouthKorea
ix
x Contributors
S.SAMUELKIM (cid:1) Center forFertilityandReproduction,American-SinoWomen’sand
Children’sHospital,Shanghai,China
MASASHIGEKUWAYAMA (cid:1) Repro-SupportMedicalResearchCentre,REPROLIFEInc.,
Tokyo,Japan
ANNANIL€aNGLAESSKER (cid:1) ProductDevelopment,NidaconInternationalAB,Mo¨lndal,
Sweden
MARKG.LARMAN (cid:1) Research,Vitrolife,Englewood,CO,USA
JUNG RYEOLLEE (cid:1) DepartmentofObstetricsandGynecology,SeoulNationalUniversity
BundangHospital,Seongnam,SouthKorea
JANA LIEBENTHRON (cid:1) CryobankattheDepartmentofGynecologicalEndocrinology
andReproductiveMedicine,UniversityClinicofBonn,Bonn,Germany
JUERGENLIEBERMANN (cid:1) FertilityCentersofIllinois,Chicago,IL,USA
JOSH LIM (cid:1) ShadyGroveFertilityCenter,Rockville,MD,USA
JUNLIU (cid:1) DepartmentofMechanicalandIndustrialEngineering,UniversityofToronto,
Toronto,ON,Canada
G.MAL (cid:1) ICAR-IndianVeterinaryResearchInstitute,Palampur,HimachalPradesh,India
PETERMALLMANN (cid:1) DepartmentofObstericsandGynecology,MedicalFaculty,
UniversityMaternalHospital,CologneUniversity,Cologne,Germany
MARKUSMONTAG (cid:1) ilabcommGmbH,St.Augustin,Germany
TETSUNORIMUKAIDA (cid:1) HiroshimaHartClinic,Hiroshima,Japan
K.MUTHUKUMAR (cid:1) ReproductiveMedicine,ChristianMedicalCollege,Vellore,
TamilNadu,India
JULIAC.NEUBAUER (cid:1) FraunhoferInstituteforBiomedicalEngineering,Sulzbach,
Germany
PRIYANKAPANGERKAR (cid:1) IVFDepartment,Rotunda—TheCenterForHumanReproduction,
Mumbai,Maharashtra,India
TEIJAT.PEURA (cid:1) GeneaBiomedx,Sydney,NSW,Australia
DEREKG.PYNE (cid:1) DepartmentofMechanicalandIndustrialEngineering,
UniversityofToronto,Toronto,ON,Canada
PATRICKQUINN (cid:1) Quinn’sIVFConsulting,ApacheJunction,AZ,USA
GOHARRAHIMI (cid:1) DepartmentofObstericsandGynecology,MedicalFaculty,University
MaternalHospital,CologneUniversity,Cologne,Germany
LAURA RIENZI (cid:1) GeneraCentersforReproductiveMedicine,ClinicalValleGiulia,
Rome,Italy
MARTAF.RIESCO (cid:1) DepartmentMolecularBiology,CellBiologyArea,UniversityofLeo´n,
Leo´n,Spain
VANESAROBLES (cid:1) DepartmentMolecularBiology,CellBiologyArea,UniversityofLeo´n,Leo´n,
Spain
TAMMIEK.ROY (cid:1) GeneaBiomedx,Sydney,NSW,Australia
RAULSANCHEZ (cid:1) DepartmentofPreclinicalScience,Center forTraslationMedicine,
FacultaddeMedicina,UniversidaddelaFrontera,Temuco,Chile
SHERMANSILBER (cid:1) InfertilityCenterofSt.Louis,St.Louis,MO,USA
B.SINGH (cid:1) RegionalStation,ICAR-IndianVeterinaryResearchInstitute,Palampur,
HimachalPradesh,India
SARABPREET SINGH (cid:1) ReproductiveMedicine,ArtemisHealthInstitute,Gurgaon,
Haryana,India
S.K.SINGLA (cid:1) AnimalBiotechnologyCentre,ICAR-NationalDairyResearchInstitute,
Karnal,Haryana,India
FRANKSTRACKE (cid:1) FraunhoferInstituteforBiomedicalEngineering,Sulzbach,Germany
Contributors xi
CHANGSUKSUH (cid:1) DepartmentofObstetricsandGynecology,SeoulNationalUniversity
BundangHospital,Seongnam,SouthKorea
YUSUN (cid:1) DepartmentofMechanicalandIndustrialEngineering,UniversityofToronto,
Toronto,ON,Canada
PANKAJTALWAR (cid:1) ARTCentre,ArmyHospital(ResearchandReferral),NewDelhi,India
MICHAELTUCKER (cid:1) ShadyGroveFertilityCenter,Rockville,MD,USA
EVATVRDA (cid:1) AmericanCenter forReproductiveMedicine,ClevelandClinic,Cleveland,
OH,USA;DepartmentofAnimalPhysiology,SlovakUniversityofAgriculture,Nitra,
SlovakRepublic
FILIPPO MARIAUBALDI (cid:1) GeneraCenter forReproductiveMedicine,ClinicalValleGiulia,
Rome,Italy
DAVIDG.VALCARCE (cid:1) DepartmentMolecularBiology,CellBiologyArea,UniversityofLeo´n,
Leo´n,Spain
MATTHEWVERMILYEA (cid:1) OvationFertility,Austin,TX,USA
YIXINXU (cid:1) DepartmentofObstetricsandGynecology,McGillUniversity,Montreal,Canada
YEHUDITNATAN (cid:1) FertilesafeLtd,NessZiona,Israel
HYEWONYOUM (cid:1) DepartmentofObstetricsandGynecology,SeoulNationalUniversity
BundangHospital,Seongnam,SouthKorea
C.ZACA` (cid:1) 9.babyFamilyandFertilityCenter,Bologna,Italy
HEIKOZIMMERMANN (cid:1) FraunhoferInstituteforBiomedicalEngineering,Sulzbach,Germany