Table Of ContentContributors to Volume 138
Article numbers are in parentheses following the names of contributors.
Affiliations listed are current.
JUNKO AMANO (64), Department of Bio- CrtERYL A. BORGMAN (9), Department of
chemistry, The Institute of Medical Sci- Pathology, University of Virginia Medical
ence, The University of Tokyo, 4-6-1 Center, Charlottesville, Virginia 80922
Shirokanedai, Minato-ku, Tokyo 108, OCIRNE CABIB (55, 56), National Institute of
Japan Diabetes and Digestive and Kidney Dis-
NAYLAK R. ANUMULA (67), Department of eases, National Institutes of Health, Be-
Biological Sciences, State University of thesda, Maryland 29802
New York at Buffalo, Buffalo, New York ROTCIV J. CHEN (33), Lilly Research Labo-
06241 ratories, Indianapolis, Indiana 58264
JANICE Au-YOUNG (56), Center for Cancer DAVID T. CHEUNG (31), Bone and Connec-
Research, Massachusetts Institute of tive Tissue Research Laboratory, Ortho-
Technology, Cambridge, Massachusetts pedic Hospital of Los Angeles, Los
93120 Angeles, California 7962-70009
OM P. BAHL (27, 28, 67), Department of Bi- HUNG-CHE CHON (51), Department of Pa-
ological Sciences, State University of thology, St. Louis University Medical
Center, St. Louis, Missouri 40136
New York at Buffalo, Buffalo, New York
06241 ERNST NNAMLEZNOC (66), lnstitut fiir -rO
ganische Chemie und Biochemie, Univer-
KAREN J. BAME (52), Department of Bio-
sitiit Bonn, D-5300 Bonn ,1 Federal Re-
chemistry, The University of Alabama at
public of Germany
Birmingham, Birmingham, Alabama
49253 DRAHCIR D. CUMMINGS (18), Department
of Biochemistry, School of Chemical Sci-
SAMUEL H. BARONDES (42, 43), Depart-
ences, University of Georgia, Athens,
ment of Psychiatry, University of -rofilaC
Georgia 20603
ain at San Francisco, San Francisco, Cal-
J. DAKOUR (24), Department of Clinical
ifornia 34149
Chemistry, University Hospital, S-221 58
MANJU BASU (51), Department of Chemis- Lund, Sweden
try, Biochemistry, Biophysics, and Molec-
PETER F. DANIEL (7), Department of Bio-
ular Biology Program, University of No-
chemistry, E. K. Shriver Center, Wal-
ert Dame, Notre Dame, Indiana 65564
tham, Massachusetts 45220
HSAHBUS BASU (5 I ,1 Department of Chem-
KAMAL K. SAD (51), Department of Chem-
istry, University of Notre Dame. Notre
istry, University of Notre Dame, Notre
Dame, Indiana 65564
Dame, Indiana 65564
EDWARD A. BAYER (35), Department of TRPTI DE (51), Indian Institute of Chemical
Biophysics, The Weizmann Institute of Biology, Calcutta ,230007 India
Science, Rehovot ,00167 Israel
KCAJ J. DlSTLER (41), Department of Inter-
PAUL D. BISHOP (60), Program ni Biochem- nal Medicine, ehT University of Michigan
istry and Biophysics, Washington State Medical School, Ann Arbor, Michigan
University, Pullman, Washington 46199 90184
xi
xii CONTRIBUTORS TO VOLUME 138
ALAN D. ELBEIN (58), Department of Bio- search, National Institutes of Health, Be-
chemistry, The University of Texas thesda, Maryland 29802
Health Science Center, San Antonio, RALPH HEIMER (19), Department of Bio-
Texas 48287 chemistry, Thomas Jefferson University,
AKKUJ FINNE (22), Department of Biochem- Philadelphia, Pennsylvania 70191
istry, University of Kuopio, SF-70211 SOLRAC B. GRESHCSRIH (59), E. A. Doisy
Kuopio, Finland Department of Biochemistry, St. Louis
ALFRED P. FISHMAN (19), Cardiovascular- University School of Medicine, St. Louis,
Pulmonary Division, Department of Med- Missouri 40136
icine, Hospital of the University of Penn- C. A. HOPPE (30), Department of Biology,
sylvania, Philadelphia, Pennsylvania ehT Johns Hopkins University, Balti-
40191 more, Maryland 81212
DLORAH M. FLOWERS (29), Department of SAMOHT C.-Y. HSlEH (13), Department of
Biophysics, ehT Weizmann Institute of Food Science and Department of Bio-
Science, Rehovot ,00167 Israel chemistry, Louisiana State University
BAIBA GILLARD (23), Department of Medi- and A & M College and ehT Louisiana
cine, Baylor College of Medicine, Hous- State University Agricultural Center,
ton, Texas 03077 Baton Rouge, Louisiana 30807
VICTOR G1NSBURG (14), Laboratory of EGROEG W. NAIDRUOJ (41), Departments of
Structural Biology, National Institute of Biological Chemistry and Internal Medi-
Diabetes and Digestive and Kidney Dis- cine, ehT University of Michigan Medical
eases, National Institutes of Health, Be- School, Ann Arbor, Michigan 90184
thesda, Maryland 29802 REDNIHOM S. KANG (55, 56), National Can-
R. C. GOLDMAN (20), Anti-infective Re- cer Institute, National Institutes of
search Division, Abbott Laboratories, Health, Bethesda, Maryland 29802
Abbott Park, Illinois 46006
REIJI KANNAGI (1), Department of Labora-
I. J. GOLDSTEIN (48, 49), Department of -iB tory Medicine, Kyoto University School
ological Chemistry, University of Michi- of Medicine, Kyoto, Japan
gan, Ann Arbor, Michigan 90184
SREDNA-LRAK NOSSLRAK (16, 17), Depart-
YRAG R. GRAy (3), Department of Chemis- ment of Medical Biochemistry, University
try, University of Minnesota, Minneapo- of ,grobetOG S-400 33 GOteborg, Sweden
lis, Minnesota 55455
K. KAWAGUCHI (30), Department t~JBiol-
HORITI-NES HAKOMORi (1, 2), Program of ogy, ehT Johns Hopkins University, Balti-
Biochemical Oncology, Fred Hutchinson more, Maryland 81212
Cancer Research Center, and Depart-
AKIRA ATABOK (6, 64), Department of Bio-
ment of Pathobiology, Microbiology, and
chemistry, ehT Institute of Medical Sci-
Immunology, University of Washington,
ence, ehT University of Tokyo, 4-6-1
Seattle, Washington 40189
Shirokanedai, Minato-ku, Tokyo 108,
KCIRTAP C. KCEBNELLAH (12), Department Japan
of Food Science and Technology, Univer-
ERODOEHT A. W. KOERNER (4), Depart-
sity of California at Davis, Davis, Califor-
ment of Pathology, College of Medicine,
ain 61659
University of lowa, Iowa City, Iowa 24225
G. HANSSON (24), Department of Medical
DNILASOR KORNFELD (46), Departments of
Chemistry, University of ,grobetOG S-400
Internal Medicine and Biological Chemis-
33 G6teborg, Sweden
try, Division of Hematology-Oncology,
VINCENT C. HASCALL (21), Bone Research Washington University School of Medi-
Branch, National Institute of Dental Re- cine, St. Louis, Missouri 01136
CONTRIBUTORS TO VOLUME 138 xiii
BART/~OMIEJ KWIATKOWSKI (65), Depart- A. LUNDBLAD (24), Department of lacinilC
ment of Physics and Biophysics, Institute Chemistry, University Hospital, S-221 58
of Medical Biology, Medical Academy, Lund, Sweden
112-08-LP Gd6nsk, Poland JOHN L. MA~NANI (14, 15, 39), Laboratory
JOHN W. KYLE (51), Department of Bio- of Structural Biology, National Institute
chemistry, St. Louis University Medical of Diabetes and Digestive and Kidney
Center, St. Louis, Missouri 40136 Diseases, National Institutes of Health,
STEPHAN LADISCH (23), Department of He- Bethesda, Maryland 29802
matology/Oncology, Department of Pedi- FRANK MALEY (62), Wadsworth Center for
atrics, School of Medicine, University of Laboratories and Research, New York
California at Los Angeles, Los Angeles, State Department of Health, Albany,
California 42009 New York 10221
ROGER A. LAINE (13), Department of Bio- M. MALIARIK (49), Department of Biologi-
chemistry and Department of Chemistry, cal Chemistry, University of Michigan,
Louisiana State University and A & M Ann Arbor, Michigan 90184
College and ehT Louisiana State Univer- ROBERT M. MAYER (57), Department of
sity Agricultural Center, Baton Rouge, Chemistry, ehT Ohio State University,
Louisiana 30807 Columbus, Ohio 01234
REIKO T. LEE (34), Department of Biology R. H. McCLUER (8), Department of Bio-
and McCollum-Pratt Institnte, ehT Johns chemistry, E. K. Shriver Center, Wal-
Hopkins University, Baltimore, Muryhmd tham, Massachusetts 45220
81212
RONALD D. McCoY (12, 54), Department of
Y. C. LEE (30, 34), Department of Biology Biological Chemistry, School of Medi-
and McColhon-Prutt Institute, ehT Johns cine, University of California at Davis,
Hopkins University, Baltimore. Maryland Davis, California 61659
81212
ARLENE J. MENCKE (31), Central Research
HAKON LEFFLER (42), Department of Psy-
Laboratories, 3M Company, St. Paul,
chiatry, University of California at San
Minnesota 33155
Francisco, San Francisco, California
34149 ROBERT K. MERKEE (18), Department of
Biochemistry, School of Chemical Sci-
L. LEIVE 1 (20), Laboratory of Structural -iB
ences, University of Georgia, Athens,
ology, National Institute of Diabetes and
Georgia 20603
Digestive and Kidney Diseases, National
Institutes of Health, Bethesda, Maryland RONALD J. MIDURA (21), Bone Research
29802 Branch, National Institute of Dental Re-
search, National Institutes of Health, Be-
STEVEN B. LEVERY (2), Program of Bio-
thesda, Maryland 29802
chemical Ontology, Fred Hutchinson
Cancer Research Center, University of RONALD L. MILLER (45), Department of
Washington, Seattle, Washington 40189 Biochemistry, Medical University of
P. LIN (30), Department of Biology, ehT South Carolina, Charleston, South Caro-
Johns Hopkins University. Baltimore. lina 52492
Maryland 81212 JAAKKO PARKKINEN (22), Department of
HALmA LIS (47), Department of Biophys- Medical Chemistry, University of Hel-
ics, The Weizmann Institute of Science, sinki, SF-00170 Helsinki, Finland
Rehovot ,00167 Israel JAMES C. PAULSON (11, 44), Department of
Biological Chemistry, School of Medi-
cine, University of California at Los
*Deceased. Angeles, Los Angeles, California 42009
XiV CONTRIBUTORS TO VOLUME 138
YRAM PEREZ (59), Department oflmmunol- SILLYHP M. NOSPMAS (19), Cardiovascular-
ogy, Scripps Clinic and Research Foun- Pulmonary Division, Department of Med-
dation, La Jolla, California 92037 icine, Hospital of the University of Penn-
YZREJ KAINYRTEP (48), Department of Bio- sylvania, Philadelphia, Pennsylvania
logical Chemistry, University of Michi- 40191
gan, Ann Arbor, Michigan 48109 Bo E. NOSSLEUMAS (50), Department of
SAMOtrT H. PLUMMER, JR. (63), Wadsworth Medical Biochemistry, University of
Center for Laboratories and Research, GOteborg, S-400 33 GOteborg, Sweden
New York State Department of Health, DARNOK SANDHOEF (26, 66), Institute fiir
Albany, New York 10221 Organische Chemie und Biochemie, Un-
SEMAJ H. DRAGETSERP (4), Department of iversitfit Bonn, D-5300 Bonn ,1 Federal
Chemistry, Yale University, New Haven, Republic of Germany
Connecticut 11560 TREBOR J. SCHAEPER (51), Department of
M. H. HTANARDNIVAR (44), Department of Chemistry, University of Notre Dame,
Surgery/Oncology, University of Califor- Notre Dame, Indiana 46556
nia at Los Angeles, Los Angeles, Califor-
ROLAND SCHAUER (10, 53), Biochemisches
nia 90024
Institiit, Christian-Albrechts-Universitdt
NONREV N. REINHOLD (5), Department of zu Kiel, D-2300 Kiel, Federal Republic of
Nutrition, Harvard School of Public Germany
Health, Boston, Massachusetts 51120
K. REORHCS (24), Laboratory of Pathology,
APRIL R. ROBBINS (37), Genetics and Bio- National Cancer Institute, National Insti-
chemistry Branch, National Institute of tutes of Health, Bethesda, Maryland
Diabetes and Digestive and Kidney Dis- 29802
eases, National Institutes of Health, Be-
RETN0fG NNAMZRAWHCS (26), Institut fiir
thesda, Maryland 20892
Organische Chemie und Biochemie, Un-
MARIE M. NOSREBOR (43), Department of iversitat Bonn, D-5300 Bonn I, Federal
Psychiatry, University of California at
Republic of Germany
San Diego, La Jolla, California 39029
NATHAN NORAHS (47), Department of Bio-
D. STREBOR (38, 49), Laboratory of Struc-
physics, The Weizmann Institute of Sci-
tural Biology, National Institute of Diabe-
ence, Rehovot 76100, Israel
tes and Digestive and Kidney Diseases,
National Institutes of Health, Bethesda, NIDDUMIKAH T. SOJAR (27), Department of
Maryland 29802 Biological Sciences, State University of
New York at Buffalo, Buffalo, New York
CALVIN F. ROLE (37), Genetics and Bio-
06241
chemistry Branch, National Institute of
Diabetes and Digestive and Kidney Dis- HARAS SPIEGEL (25), Membrane Biochemis-
eases, National Institutes of Health, Be- try Section, Developmental and Meta-
thesda, Maryland 29802 bolic Neurology Branch, National Insti-
tute of Neurological and Communicative
YRAG N. SREGOR (11), Department of Phar-
Disorders and Stroke, National Institutes
macology, College of Medicine, Univer-
sity of lowa, Iowa City, Iowa 52242 of Health, Bethesda, Maryland 29802
DRANOEL H. ROME (52), Department of Bi- NEVETS L. SPITALNIK (14, 40), Department
ological Chemistry, School of Medicine, of Pathology and Laboratory Medicine,
University of California at Los Angeles, University of Pennsylvania, Philadelphia,
Los Angeles, California 42009 Pennsylvania 40191
ECNERALC A. RYAN (60), Institute of Bio- ALEMAP YELNATS (36), Department of Cell
logical Chemistry, Washington State Uni- Biology, Albert Einstein College of Medi-
versity, Pullman, Washington 0436-46199 cine, Bronx, New York 16401
CONTRIBUTORS TO VOLUME 138 XV
NAHPETS STIRM (65), Biochemisches lnsti- ERIC R. VIMR (12), Department of Veteri-
tut am Klinikum der Universitgit, D-6300 nary Pathobiology, College of Veterinary
Giessen, Federal Republic of Germany Medicine, University of Illinois, Urbana,
SALKCIN GREBMORTS (17), Department of Illinois 10816
Medical Biochemistry, University of DNANAMERP V. WAGH (67), Department of
,grobetOG S-400 33 GOteborg, Sweden Biological Sciences, State University of
KUNIHIKO SUZUKI (61), Departments of New York at Buffalo, Buffalo, New York
Neurology and Psychiatry, Biological 06241
Sciences Research Center, School of ORIHOYIK EBANATAW (I), Depurlmt, nl ")~
Medicine, University of North Carolina, Biochemistry. Shigei Medical Research
Chapel Hill, North Carolina 41572 Institute, Okayuma, Japan
SEIICHI IKASAKAT (6), Department of Bio- MEIR WILCHEK (35), Department of Bio-
chemistry, The Institute of Medical Sci- physics, ehT Weizmann Institute of Sci-
ence, The University of Tokyo, 4-6-I ence, Rehovot ,00167 Israel
Shirokanedai, Minato-ku, Tokyo 108,
FINN WOLD (31), Department of Biochemis-
Japan
try and Molecular Biology, University of
YNOHTNA L. TARENTINO (63), Wadsworth Texas Medical School, Houston, Texas
Center for Laboratories and Research, 72277
New York State Department of Health,
OKUSTAK AT1HSAMAY (6), Department of
Albany, New York 10221
Biochemistry, Kobe University School of
ARAWSEGAN R. ARUKATOHT (28), Depart-
Medicine, 7 Kusunoki-cho, Chuo-ku,
ment of Biological Sciences, State Uni-
Kobe 650, Japan
versity of New York at Buffalo, Buffalo,
New York 06241 SAU-CHI BETTY NAY (32), Biochemical Re-
search Division, Lilly Research Laborato-
SHERIDA TOLLEFSEN (46), Department of
ries, Eli Lilly & Company, Indianapolis,
Pediatrics, Washington University School
Indiana 58264
of Medicine, St. Louis, Missouri 01136
IKASAM ATIHSIGANAY (21), Bone Research
TREBOR B, TRIMBLE (62), Wadsworth Cen-
Branch, National Institute of Dental Re-
ter for Laboratories and Research, New
search, National Institutes of Health, Be-
York State Department of Health, Al-
thesda, Maryland 29802
bany, New York 10221
CIREDERF A. TROY (12, 54), Department of WILLIAM W. YOUNG, JR. (9), Department
Biological Chemistry, School of Medi- of Pathology, University of Virginia Med-
cine, University of California at Davis, ical Center, Charlottesville, Virginia 80922
Davis, California 61659 TREBOR K. YU (4), Departments of Neurol-
TREBOR J. TRUMBLY (62), Department of ogy and Molecular Biophysics and Bio-
Biochemistry, Medical College of Ohio, chemistry, School of Medicine, Yale Uni-
Toledo, Ohio 99634 versity, New Haven, Connecticut 01560
M. D. ULLMAN (8), Research Service and D. ZOPF (24), Laboratory of Pathology, Na-
Geriatric Research, Education and -inilC tional Cancer Institute, National Insti-
cal Center, ENRM Veterans Hospital, tutes of Health, Bethesda, Maryland
Bedford, Massachusetts 03710 29802
Preface
New methods for studying the composition, synthesis, and degrada-
tion of complex carbohydrates are rapidly being developed. This volume
contains some of the advances that have appeared in the literature since
the publication in 2891 of Volume .38 As were the previous volumes in
this series that deal with complex carbohydrates, this volume is divided
into five sections: Analytical Methods, Preparations, Carbohydrate-Bind-
ing Proteins, Biosynthesis, and Degradation. I would welcome sugges-
tions for articles on important areas of research for future supplements in
this series.
VICTOR GINSBURG
iivx
METHODS IN ENZYMOLOGY
EDITED YB
Sidney P. Colowick and Nathan O. Kaplan
VANDERBILT UNIVERSITY DEPARTMENT OF CHEMISTRY
SCHOOL OF MEDICINE UNIVERSITY OF CALIFORNIA
NASHVILLE, TENNESSEE AT SAN DIEGO
LA JOLLA, CALIFORNIA
.I Preparation and Assay of Enzymes
II. Preparation and Assay of Enzymes
III. Preparation and Assay of Substrates
IV. Special Techniques for the Enzymologist
.V Preparation and Assay of Enzymes
.IV Preparation and Assay of Enzymes )deunitnoC(
Preparation and Assay of Substrates
Special Techniques
VII. Cumulative Subject Index
xix
METHODS IN ENZYMOLOGY
EDITORS-IN-CHIEF
Sidney P. Colowick and Nathan .O Kaplan
VOLUME VllI. Complex Carbohydrates
Edited by ELIZABETH F. NEUFELD AND VICTOR GINSBURG
VOLUME IX. Carbohydrate Metabolism
Edited by WILLIS A. WOOD
VOLUME X. Oxidation and Phosphorylation
Edited by RONALD W. ESTABROOK AND MAYNARD E. PULLMAN
VOLUME XI. Enzyme Structure
Edited by C. H. W. HIRS
EMULOV XII. Nucleic Acids (Parts A and B)
Edited by LAWRENCE GROSSMAN AND KIVlE MOLDAVE
VOLUME XIII. Citric Acid Cycle
Edited by J. M. LOWENSTEIN
EMULOV XIV. Lipids
Edited by J. M. LOWENSTEIN
VOLUME XV. Steroids and Terpenoids
Edited by RAYMOND B. CLAYTON
VOLUME XVI. Fast Reactions
Edited by KENNETH KUSTIN
VOLUME XVII. Metabolism of Amino Acids and Amines (Parts A and B)
Edited by HERBERT TABOR DNA CELIA WHITE ROBAT
ixx
xxii
METHODS IN ENZYMOLOGY
EMULOV XVIII. Vitamins and Coenzymes (Parts A, B, and C)
Edited yb DLANOD B. KCIMROCCM DNA LEUMEL D. THGIRW
EMULOV XIX. Proteolytic Enzymes
Edited yb EDURTREG E. NNAMLREP DNA OLZSAL DNAROL
EMULOV XX. Nucleic Acids and Protein Synthesis (Part C)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXI. Nucleic Acids (Part D)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXII. Enzyme Purification and Related Techniques
Edited by MAILLIW B. VBOKAJ
EMULOV XXIII. Photosynthesis (Part A)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXlV. Photosynthesis and Nitrogen Fixation (Part B)
Edited by YNOHTNA NAS ORTEIP
EMULOV XXV. Enzyme Structure (Part B)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVI. Enzyme Structure (Part C)
Edited by C. H. W. SR1H DNA EGRES N. FFEHSAMIT
EMULOV XXVII. Enzyme Structure (Part D)
Edited by C. H. W. HIRS DNA EGRES N. FFEHSAMIT
EMULOV XXVIII. Complex Carbohydrates (Part B)
Edited by ROTCIV GRUBSNIG
EMULOV XXIX. Nucleic Acids and Protein Synthesis (Part E)
Edited by ECNERWAL NAMSSORG DNA KIVIE EVADLOM
EMULOV XXX. Nucleic Acids and Protein Synthesis (Part F)
Edited by KIVIE EVADLOM DNA ECNERWAL NAMSSORG
EMULOV XXXI. Biomembranes (Part A)
Edited yb YENDIS REHCSIELF DNA RETSEL REKCAP
xxiii
METHODS IN ENZYMOLOGY
VOLUME XXXII. Biomembranes (Part B)
Edited by SIDNEY FLEISCHER DNA LESTER REKCAP
VOLUME XXXIII. Cumulative Subject Index Volumes I-XXX
Edited by MARTHA G. DENNIS DNA EDWARD A. DENNIS
VOLUME XXXIV. Affinity Techniques (Enzyme Purification: Part B)
Edited by WILLIAM B. YBOKAJ DNA MEIR WILCHEK
VOLUME XXXV. Lipids (Part )B
Edited by JOHN M. LOWENSTEIN
VOLUME XXXVI. Hormone Action (Part A: Steroid Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
VOLUME XXXVII. Hormone Action (Part B: Peptide Hormones)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
VOLUME XXXVIII. Hormone Action (Part C: Cyclic Nucleotides)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
VOLUME XXXIX. Hormone Action (Part D: Isolated Cells, Tissues, and
Organ Systems)
Edited by JOEL G. HARDMAN DNA BERT W. O'MALLEY
VOLUME XL. Hormone Action (Part E: Nuclear Structure and Function)
Edited by BERT W. O'MALLEY DNA JOEL G. NAMDRAH
VOLUME XLI. Carbohydrate Metabolism (Part )B
Edited by W. A. WOOD
VOLUME XLII. Carbohydrate Metabolism (Part )C
Edited by W. A. WOOD
VOLUME XLIII. Antibiotics
Edited by JOHN H. HASH
VOLUME XLIV. Immobilized Enzymes
Edited by KLAUS HCABSOM
VOLUME XLV. Proteolytic Enzymes (Part B)
Edited by LASZLO LORAND
