COMBINING ABILITY ANALYSIS FOR YIELD, ITS COMPONENTS AND PROTEIN CONTENT IN ...

111 Pages·2015·2.78 MB·English

“COMBINING ABILITY ANALYSIS FOR YIELD, ITS
COMPONENTS AND PROTEIN CONTENT IN
LENTIL, (Lens culinaris Medik.L.).”

M. Sc. (Ag.) Thesis

by

Umakant Banjare

DEPARTMENT OF GENETICS AND PLANT BREEDING
COLLEGE OF AGRICULTURE, RAIPUR
FACULTY OF AGRICULTURE

INDIRA GANDHI KRISHI VISHWAVIDYALAYA
RAIPUR (C.G.)
2015
“COMBINING ABILITY ANALYSIS FOR YIELD, ITS
COMPONENTS AND PROTEIN CONTENT IN
LENTIL, (Lens culinaris Medik. L.).”
Thesis

Submitted to the
Indira Gandhi Krishi Vishwavidyalaya, Raipur (C.G.)

by

Umakant Banjare

IN PARTIAL FULFILMENT OF THE REQUIREMENTS
FOR THE DEGREE OF

Master of Science

in
Genetics and Plant Breeding

U.E.ID. 20131418429 ID No. 120113067
JULY, 2015

ACKNOWLEDGEMENT

“Education plays a vital role in personal and social development and teacher plays a fundamental
role in imparting education. Teachers have crucial role in shaping young manforce not only to face the
challenges with confidence but also to build up it with aim and responsibility. There is no substitute for
teacher pupil relationship”. I take this golden opportunity to express my heartful humble and deepest sense
of gratitude to those who helped me to complete my research possible. These words are small
acknowledgement but never fully recompensed for their great help and co-operation.

Above all my humble and wholehearted prostration before Almighty and Omnipresent “God” for
sprinkling his unprecedented favoured upon me to reach the acme of success and always with me for
improving the confidence and determination in my whole life. How can I express my thanks to “God” because
there is no any word to express it? So, I can only precept to almighty “God”.

From the core of my heart I owe to my Hon’ble guide and Chairman of my Advisory Committee

Dr. H.C. Nanda, Principal Scientist, MULLaRP, Department of Genetics and Plant Breeding, IGKV,
Raipur (C.G.). I express my cordial thanks to him for invaluable inspiring guidance, unfailing
encouragement, suggestions, scholarly advice, sympathetic attitude, and sustained support throughout the
investigation and preparation of this manuscript. The parental affection, which I enjoyed from him during
past years, will have a life-long impression in my heart.

I would like to express my sincere regard and heartfelt gratitude to respected teachers and members
of my Advisory Committee Dr. S.K...Nair Scientist (Department of Genetics and Plant Breeding), Dr. N.
Khare, Associate professor Pl. Pathology and Dr. R.R. Saxena, Associate Professor (Department of
Agriculture Statistics, and Social Science (L.), Dr. A.K..Geda Scientist (Deptt. Of plant physiology,
Agriculture biochemistry, Medicinal and aromatic plants) for their critical suggestions and regular
encouragement during the course of this investigation.

I have immense pleasure in expressing my whole heartful thanks to Dr. A.K.Sarawagi, Professor
and Head, Department of Genetics and Plant Breeding, for providing all the facilities and help as well as of
his valuable supervision in all the academic and departmental issues during study.

I am highly obliged to Dr. S.K.Patil, Hon’ble Vice-Chancellor, IGKV, Raipur, Dr. S.S. Shaw,
Director of Instructions, Dr. J.S.Urkurkar, Director, Research Services, Dr. M.P. Thakur, Director
Extension Services, Dr. S.S. Sengar, Dean Student Welfare, Dr. S.S. Rao, Dean, College of Agriculture,
Raipur, and Sh. K.C Painkra, Registrar, Raipur, IGKV, Raipur for providing all necessary facilities during
the course of this study.

I am deeply indebted with deep sense of gratitude for the highly becknable exceptional guidance of
Dr. Rajeev Shrivastava, Scientist, Dr. N.K.Rastogi, Dr. R.N. Sharma, Dr. D. Sharma, Dr. N. Mehta, Dr.
N.K. Motiramani, Dr. R. Verma, Shri P.L. Johnson, Shri S.K. Nag, Dr. Ritu R. Saxena, Mrs. P.
Chaudhary, Dr.A.Tirkey, Mrs. Mangla Parikh, Mrs.Bhawna Sharma, Mrs. Mayuri Sahu for their
encouragement and constant help throughout my studies. I extent my thanks to Jagdish, Surendra, Balla and
Karan who helped me a lot during office and field work.

CONTENTS

Chapter Title PageNo.
ACKNOWLEDGEMENT iii
LIST OF TABLES viii
LIST OF FIGURES ix
LIST OF NOTATIONS x
LIST OF ABBREVIATIONS xi
ABSTRACT xii
I. INTRODUCTION 1
II. REVIEW OF LITERATURE 5
2.1 Genetic Variability 5
2.2 Heritability and genetic advance 9
2.3 Combining ability 11
2.4 Gene action 15
2.5 Heterosis 16
III MATERIALS AND METHODS 20
3.1 Experimental materials 20
3.2 Experimental design 20
3.3Observations and collection of data 22
3.3.1 Days to flower initiation 22
3.3.2 Days to 50 per cent flowering 22
3.3.3 Days to maturity 22
3.3.4 Plant height (cm) 22
-1
3.3.5 Number of primary branches plant 22
-1
3.3.6 Number of secondary branches plant 22
-1
3.3.7 Number of pods plant 23
-1
3.3.8 Number of seeds pods 23
-1
3.3.9 Number of seeds plant 23
3.3.10 100-seed weight (g) 23
3.3.11 Seed yield per plant (g) 23
3.3.12 Protein content (%) 23
3.3.13 Biological yield per plant (g) 23
3.3.14 Harvest index (%) 24
3.3.15 Wilt Incidence score (%) 24
3.3.16 Dal recovery (%) 24
3.4 Statistical analysis 24
3.4.1 Analysis of variance 24
3.4.2 Parameters of variation 26
3.4.2.1 Mean 26
3.4.2.2 Range 27
3.4.3 Genetic Analysis 27
3.4.3.1 Variability 27
3.4.3.1.1 Phenotypic coefficient of variation 27
3.4.3.1.2 Genotypic coefficient of variation 27
3.4.3.2 Heritability 28
3.4.3.3 Genetic advance 29
3.4.3.3.1 Genetic advance as % of mean 30

v
3.4.3.4 Heterosis 30
3.4.3.5 Combining Ability Analysis 31
3.5 Quality analysis 36
3.5.1 Protein content 36
IV RESULTS AND DISCUSSION 37
4.1 Genetic Variability 37
4.1.1 Analysis of variance 38
4.1.2 Mean, Range and Coefficient of variance 38
4.1.2.1 Mean and Range 38
4.1.2.1.1 Days to flower initiation 39
4.1.2.1.2 Days to 50% flowering 39
4.1.2.1.3 Days to maturity 39
4.1.2.1.4 Plant height(cm) 39
4.1.2.1.5 Number of primary branches 39
-1
plant
4.1.2.1.6 Number of secondary branches 39
-1
plant
1
4.1.2.1.7 Number of pods plant- 39
1
4.1.2.1.8 Number of seeds pod- 39
1
4.1.2.1.9 Number of seeds plant- 39
4.1.2.1.10 100-seed weight 39
4.1.2.1.11 Protein content(%) 40
4.1.2.1.12 Biological yield(g) 40
-1
4.1.2.1.13 Seed yield plant 40
4.1.2.1.14 Harvest index(%) 40
4.1.2.1.15 Wilt incidence score(%) 40
4.1.2.1.16 Dal Recovery(%) 40
4.1.2.2 Genotypic and Phenotypic coefficients of 40
variation
4.1.3 Heritability and genetic advance 43
4.2Combining Ability and Gene Action 46
4.2.1 Analysis of variance for combining ability 46
4.2.2 Estimates of GCA & SCA effects and Gene action 46
4.2.2.1 Days to flower initiation 47
4.2.2.2 Days to 50% flowering 50
4.2.2.3 Days to maturity 51
4.2.2.4 Plant height (cm) 51
-1
4.2.2.5 Number of primary branches plant 52
-1
4.2.2.6 Number of secondary branches plant 53
-1
4.2.2.7 Number of pods plant 53
-1
4.2.2.8 Number of seeds pod 53
-1
4.2.2.9 Number of seeds plant 54
4.2.2.10 100-seed weight (g) 54
4.2.2.11 Protein content (%) 55
-1
4.2.2.12 Biological yield plant (g) 55
-1
4.2.2.13 Seed yield plant 56
4.2.2.14 Harvest Index (%) 57
4.2.2.15 Wilt Incidence score (%) 58
vi
4.2.2.16Dal Recovery (%) 58
4.3 Heterosis 59
4.3.1 Days to flower initiation 59
4.3.2 Days to 50% flowering 60
4.3.3 Days to maturity 60
4.3.4 Plant height(cm) 60
-1
4.3.5 Number of primary branches plant 67
-1
4.3.6 Number of secondary branches plant 67
-1
4.3.7 Number of pods plant 68
-1
4.3.8 Number of seeds pod 69
-1
4.3.9 Number of seeds plant 69
4.3.10 100-seed weight (g) 70
4.3.11 Protein content (%) 70
-1
4.3.12 Biological yield plant (g) 71
-1
4.3.13 Seed yield plant (g) 71
4.3.14 Harvest Index (%) 72
4.3.15 Wilt Incidence score (%) 72
4.3.16 Dal Recovery (%) 73
V SUMMARY AND CONCLUSION 74
REFERENCES 82
APPENDICES 90
Appendix A 90
Appendix B 91
VITA 92

vii
LIST OF TABLES

TABLE NO. PARTICULARS PAGE
NO.
3.1 Skeleton of analysis of variance for Randomized Block 26
Design (RBD)
3.2 Different Categories of Heritability 29
3.3 Different Categories of Genetic advance 30
3.4 ANOVA for combining ability analysis 33
4.1.a Mean sum squares due to genotypes for seed yield, its 38
components and protein content in lentil
4.1.b Genetic parameters of variation for yield and its components 42
in lentil
4.2.a ANOVA for combining ability of seed yield, its components 47
and seed protein content of 6-parent half diallel cross in
lentil
4.2.b Estimates of General Combing ability effects (gi) in 6-parent 48
half diallel cross for seed yield, its components and seed
protein content in lentil
4.2.c Estimates of Specific Combining ability effects(SCA) in 6- 49
parent half diallel cross for seed yield, its components and
seed protein content in lentil
4.2.d Components of GCA, SCA and Predictability ratios for seed 50
yield, its components and protein content of 6-parent half
diallel cross in lentil
4.3.1 Anova for 6 parents in estimation of heterosis 61
4.3.2 Anova for 15 F Crosses in estimation of heterosis 62
1
4.3.3.a Heterosis over mid parent, better parent and standard variety 63
for seed yield, its components and seed protein content in 6-
parent half diallel cross combinations in lentil
4.3.3.b Heterosis over mid parent, better parent and standard variety 64
for seed yield, its components and seed protein content in 6-
parent half diallel cross combinations in lentil
4.3.3.c Heterosis over mid parent, better parent and standard variety 65
for seed yield, its components and seed protein content in 6-
parent half diallel cross combinations in lentil
4.3.3.d Heterosis over mid parent, better parent and standard variety 66
for seed yield, its components and seed protein content in 6-
parent half diallel cross combinations in lentil

viii