Table Of ContentBiological ScienceS/Biomedical engineering SerieS in Cellular and CliniCal imaging
ammasi Periasamy, series editor
Coherent Raman
Scattering
Microscopy
Coherent Raman
Scattering
Microscopy
Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows
label-free imaging of living cells and tissues at video rate by enhancing the weak Raman
signal through nonlinear excitation. Edited by pioneers in the field and with contributions
from a distinguished team of experts, Coherent Raman Scattering Microscopy explains
how CRS can be used to obtain a point-by-point chemical map of live cells and tissues.
In color throughout, the book starts by establishing the foundation of CRS microscopy. It
discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman
spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS
microscopy. The text then provides important technical aspects of CRS microscopy,
including microscope construction, detection schemes, and data analyses. It concludes
with a survey of applications that demonstrate how CRS microscopy has become a
valuable tool in biomedicine.
Due to its label-free, noninvasive examinations of living cells and organisms, CRS
microscopy has opened up exciting prospects in biology and medicine—from the
mapping of 3D distributions of small drug molecules to identifying tumors in tissues.
An in-depth exploration of the theories, technology, and applications, this book shows Edited by
how CRS microscopy has impacted human health and will deepen our understanding of
Ji-Xin Cheng
life processes in the future.
K13060 Xiaoliang Sunney Xie
ISBN: 978-1-4398-6765-5
90000
9 781439 867655
K13060_Cover_mech.indd 1 8/28/12 9:46 AM
Coherent Raman
Scattering
Microscopy
Series in Cellular and Clinical Imaging
Series Editor
Ammasi Periasamy
PUBLISHED
Coherent Raman Scattering Microscopy
edited by Ji-Xin Cheng and Xiaoliang Sunney Xie
FORTHCOMING
The Fluorescent Protein Revolution
edited by Richard N. Day and Michael W. Davidson
Second Harmonic Generation Imaging
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Imaging in Cellular & Tissue Engineering
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Natural Biomarkers for Cellular Metabolism:
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Optical Probes in Biology
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SerieS in Cellular and CliniCal imaging
ammasi Periasamy, series editor
Coherent Raman
Scattering
Microscopy
Edited by
Ji-Xin Cheng
Xiaoliang Sunney Xie
Boca Raton London New York
CRC Press is an imprint of the
Taylor & Francis Group, an informa business
Front Cover: Multimodal Imaging of the central nervous system, courtesy of Ji-Xin Cheng. Back Cover: The stimulated Raman
scattering image of a mouse brain cross-section, showing the distributions of protein (blue) and lipid (green), from X. Sunney
Xie’s group. Imaging courtesy of Minbiao Ji of Harvard University and Daniel Orringer of Brigham and Women’s Hospital.
MATLAB® and Simulink® are trademarks of The MathWorks, Inc. and are used with permission. The MathWorks does not war-
rant the accuracy of the text or exercises in this book. This book’s use or discussion of MATLAB® and Simulink® software or
related products does not constitute endorsement or sponsorship by The MathWorks of a particular pedagogical approach or
particular use of the MATLAB® and Simulink® software.
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Version Date: 20120725
International Standard Book Number-13: 978-1-4398-6766-2 (eBook - PDF)
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v
Contents
Series Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi
Editors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvii
Part I theory
1 theory of Coherent raman Scattering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Eric Olaf Potma and Shaul Mukamel
2 Coherent raman Scattering under tightly Focused Conditions . . . . . . . . . . . .43
Eric Olaf Potma, Xiaoliang Sunney Xie, andreas Volkmer, and Ji-Xin Cheng
Part II Platforms
3 Construction of a Coherent raman Microscope . . . . . . . . . . . . . . . . . . . . . . . . .79
Brian G . Saar and Xiaoliang Sunney Xie
4 Stimulated raman Scattering Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .99
Christian Freudiger and Xiaoliang Sunney Xie
5 Femtosecond versus Picosecond Pulses for Coherent raman Microscopy . . . . . .121
Mikhail N . Slipchenko, Delong Zhang, and Ji-Xin Cheng
6 Miniature Coherent raman Probes for In Vivo Biomedical Imaging . . . . . . .137
Gangjun Liu, Mihaela Balu, Zhongping Chen, and Eric Olaf Potma
7 Wide-Field CarS-Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .161
alexander Jesacher, Gregor thalhammer, Stefan Bernet,
and Monika ritsch-Marte
8 Vibrational Spectromicroscopy by Coupling Coherent raman Imaging
with Spontaneous raman Spectral analysis . . . . . . . . . . . . . . . . . . . . . . . . . . 189
Mikhail N . Slipchenko and Ji-Xin Cheng
vi
9 Coherent Control in CarS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .207
Jonathan M . Levitt, Ori Katz, and Yaron Silberberg
10 Fourier transform CarS Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .237
Jennifer P . Ogilvie
11 CrS with alternative Beam Profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253
Varun raghunathan, Hyunmin Kim, Stephan Stranick, and Eric Olaf Potma
12 Vibrational Phase Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .277
Martin Jurna, Cees Otto, and Herman L . Offerhaus
13 Multiplex CarS Microscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .291
James P .r . Day, Katrin F . Domke, Gianluca rago, Erik M . Vartiainen,
and Mischa Bonn
14 Interferometric Multiplex CarS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .309
Sang-Hyun Lim
15 Photonic Crystal Fiber–Based Broadband CarS Microscopy . . . . . . . . . . . . .329
Marcus t . Cicerone, Young Jong Lee, Sapun H . Parekh, and Khaled a . aamer
16 Multiplex Stimulated raman Scattering Microscopy . . . . . . . . . . . . . . . . . . . .353
Dan Fu and Xiaoliang Sunney Xie
Part III applications
17 Imaging Myelin Sheath Ex Vivo and In Vivo by CarS Microscopy . . . . . . . . .389
Yan Fu, Yunzhou (Sophia) Shi, and Ji-Xin Cheng
18 Imaging Lipid Metabolism in Caenorhabditis elegans and Other
Model Organisms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .405
Helen Fink, Christian Brackmann, and annika Enejder
19 Lipid-Droplet Biology and Obesity-related Health risks . . . . . . . . . . . . . . . . .421
thuc t . Le
20 White Matter Injury: Cellular-Level Myelin Damage Quantification
in Live animals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .439
Erik Bélanger, F .P . Henry, r . Vallée, M .a . randolph, I .E . Kochevar,
J .M . Winograd, Charles P . Lin, and Daniel Côté
vii
21 CarS Microscopy Study of Liquid Crystals . . . . . . . . . . . . . . . . . . . . . . . . . . . .457
Heung-Shik Park and Oleg D . Lavrentovich
22 Live Cell Imaging by Multiplex CarS Microspectroscopy . . . . . . . . . . . . . . . .479
Hideaki Kano
23 Coherent raman Scattering Imaging of Drug Delivery Systems . . . . . . . . . .505
Ling tong and Ji-Xin Cheng
24 applications of Stimulated raman Scattering Microscopy . . . . . . . . . . . . . . .519
Christian Freudiger, Daniel a . Orringer, and Xiaoliang Sunney Xie
25 applications of Coherent anti-Stokes raman Spectroscopy Imaging
to Cardiovascular Diseases . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .537
Han-Wei Wang, Michael Sturek, and Ji-Xin Cheng
26 applications of CarS Microscopy to tissue Engineering . . . . . . . . . . . . . . . .547
annika Enejder and Christian Brackmann
27 Dietary Fat absorption Visualized by CarS Microscopy . . . . . . . . . . . . . . . . .569
Kimberly K . Buhman
